Scientific evidence classification · Phase 6A preserved + Phase 6B additions

PWWP Compounds
& Bioactivity

Explore experimentally studied preparations separately from deposited research ligands, buffers, ions and unresolved chemical evidence. Every role is source- and target-context specific.

Explore studied compounds Structural & Binding Landscape →

Different denominators · 26 October 2026

38studied preparation profiles · 26 baseline + 12 new

109profiles with PWWP research-ligand geometry

93source assay / association observations · 80 baseline + 13 new

Combined Phase 6A + Phase 6B denominators. These groups overlap; the 109 structurally source-tagged research ligands are unchanged. Deposited components and reagents are not automatically biochemical binders.

Search compounds & identifiersProteinEvidence view

Specific PWWP domainExact PWWP-proximal deposited siteChemical identity

Assay, source & experimental-context filters

Assay method Endpoint Reported relation Exact concentration min (µM)Exact concentration max (µM) Publication / source databasePublication yearAssay organismTested constructVerification status / scope Missing experimental detailAny associated PDB structure Inventory scopeSort

Assay filters must match the same observation. Concentration ranges require one endpoint and exact positive measurements; inequalities and qualitative findings remain searchable separately.

Phase 6B · source-reviewed additions (separate from the Phase 6A snapshot)

13 matching new observations · 12 preparations

Keep source-level IC50, Kd, and whole-protein DC50 separate; these records were not included in the original 80.

Filter all Phase 6B assay contexts

Preparation	Target scope	Original assay	Primary source
UNC10088	NSD2 PWWP1, exact domain	IC50 =248 ± 22 nM TR-FRET competitive displacement of biotinylated UNC7096	10.1038/s41467-026-72235-9 Figure 2h and Supplementary Figure 3D
UNC10088	NSD2 PWWP1, exact domain	IC50 =101 ± 8 nM TR-FRET competitive displacement of biotinylated UNC7096	10.1038/s41467-026-72235-9 Figure 2h, Supplementary Figure 3D and Results
UNC10415667	NSD2 protein, cellular only	DC50 =460 nM Cellular HiBiT protein-degradation dose response	10.1038/s41467-026-72235-9 Figure 3e legend and Results
UNC12149	NSD2 protein, cellular only	DC50 =120 nM Cellular HiBiT protein-degradation dose response	10.1038/s41467-026-72235-9 Figure 4h legend and Results
SYC2	NSD3 PWWP, domain instance unresolved	Kd =0.07 ± 0.01 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher compound table row 28; SPR Kd (µM) column, corroborated by abstract
SYB7	NSD3 PWWP, domain instance unresolved	Kd >20 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 20; SPR Kd (µM) column
SYB10	NSD3 PWWP, domain instance unresolved	Kd =0.11 ± 0.07 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 23; SPR Kd (µM) column
SYB11	NSD3 PWWP, domain instance unresolved	Kd =0.15 ± 0.03 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 24; SPR Kd (µM) column
SYC1	NSD3 PWWP, domain instance unresolved	Kd =0.13 ± 0.04 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 27; SPR Kd (µM) column
SYC3	NSD3 PWWP, domain instance unresolved	Kd =0.32 ± 0.11 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 29; SPR Kd (µM) column
SYC5	NSD3 PWWP, domain instance unresolved	Kd =0.18 ± 0.03 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 31; SPR Kd (µM) column
SYC6	NSD3 PWWP, domain instance unresolved	Kd =0.25 ± 0.06 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 32; SPR Kd (µM) column
SYC7	NSD3 PWWP, domain instance unresolved	Kd =7.69 ± 0.32 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 33; SPR Kd (µM) column

Evidence classification · exact source records

132 matching profiles

80 matching assay / association observations. Counts here are profiles, not unique assay events or deposited ligand instances; views overlap.

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Preparation	Tested target & construct	Method / endpoint	Original observation	Conditions & uncertainty	Source & verification
BPPPREP:BPPNegative / censored	BRPF1 PWWP1 Construct not curatedHomo sapiens	qualitativeBinding selectivity; assay method not verified in Figure S2qualitative binding	Source reports non-detectable affinity in the tested selectivity comparison.	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification No concentration limit, numeric Kd, assay method or construct boundaries assigned without original Supplementary Figure S2. Protein has the named PWWP domain; this qualitative observation is not an unbounded inactivity claim.	PMID:39031937 · 2024Official publisher full HTML independently inspected: Methods, Results and Figures 1–4 captions; original Supplementary Figure S2 was not retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0030", "assay_type": "Binding selectivity; assay method not verified in Figure S2", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "compound_name": "BPP", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:P55201:PWWP1", "endpoint": "qualitative", "evidence_class": "qualitative_binding", "evidence_location": "Results: selectivity paragraph referring to Figure S2", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.14981", "gene": "BRPF1", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": true, "negative_evidence_scope": "Non-detectable binding in the source experimental comparison; assay limits not recovered.", "numeric_transcription_status": "qualitative_or_not_reported", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:BPP", "protein_id": "UP:P55201", "publication_year": 2024, "qualitative_result": "Source reports non-detectable affinity in the tested selectivity comparison.", "reference_id": "PMID:39031937", "remaining_uncertainty": "No concentration limit, numeric Kd, assay method or construct boundaries assigned without original Supplementary Figure S2. Protein has the named PWWP domain; this qualitative observation is not an unbounded inactivity claim.", "replicates": null, "retrieval_date": "2026-10-26", "role": "experimentally_studied_ligand", "source_id": "P5:S:WEI2024", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Official publisher full HTML independently inspected: Methods, Results and Figures 1\u20134 captions; original Supplementary Figure S2 was not retrieved", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
Bis-TrisPREP:BIS-TRIS	DNMT3A PWWP Construct not curatedHomo sapiens	Qualitative / associationNo pharmacological activity assignedstructural occupancy association	No quantitative observation curated	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Crystallization reservoir contains 0.1 M Bis-Tris; not an affinity assay Conditions {} Qualification Pocket occupancy in high-concentration crystallization buffer is not evidence of potency.	PMID:21720545 · 2011local_primary_full_text_XML; Tables 1-2, crystallization methods and ligand Results inspected Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0033", "assay_type": "No pharmacological activity assigned", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "resolve_missing_CCD_to_BTB; retain_buffer_role", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0033\", \"assay_type\": \"No pharmacological activity assigned\", \"ccd_id\": null, \"compound_name\": \"Bis-Tris\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results: Trimethylated lysine histone recognition; crystallization methods\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/\", \"gene\": \"DNMT3A\", \"interpretation\": \"Observed aromatic-cage occupancy by crystallization buffer. Do not classify as a validated pharmacological ligand.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"3LLR\", \"reference_id\": \"PMID:21720545\", \"role\": \"crystallization_buffer\", \"uncertainty\": null, \"units\": null, \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "Crystallization reservoir contains 0.1 M Bis-Tris; not an affinity assay", "ccd_id": "BTB", "ccd_mapping_source": null, "chemical_id": "INCHIKEY:OWMVSZAMULFTJU-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "Bis-Tris", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:Q9Y6K1:PWWP1", "endpoint_label_status": null, "evidence_class": "structural_occupancy_association", "evidence_location": "Results: Trimethylated lysine histone recognition; crystallization methods", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/", "gene": "DNMT3A", "interpretation": "Observed aromatic-cage occupancy by crystallization buffer. Do not classify as a validated pharmacological ligand.", "molecular_entity_type": "buffer_additive", "numeric_transcription_status": "not_a_quantitative_affinity_record", "numeric_value_inferred_from_structure": 0, "pH": 6.0, "pdb_id": "3LLR", "preparation_id": "PREP:BIS-TRIS", "protein_id": "UP:Q9Y6K1", "qualitative_result": null, "reference_id": "PMID:21720545", "remaining_uncertainty": "Pocket occupancy in high-concentration crystallization buffer is not evidence of potency.", "replicates": null, "role": "crystallization_buffer", "source_component_url": null, "source_id": "S07", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML; Tables 1-2, crystallization methods and ligand Results inspected", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_full_text_sections_verified" }
Bis-TrisPREP:BIS-TRIS	DNMT3B PWWP Construct not curatedHomo sapiens	Qualitative / associationNo pharmacological activity assignedstructural occupancy association	No quantitative observation curated	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Crystallization reservoir contains 0.1 M Bis-Tris; not an affinity assay Conditions {} Qualification Pocket occupancy in high-concentration crystallization buffer is not evidence of potency.	PMID:21720545 · 2011local_primary_full_text_XML; Tables 1-2, crystallization methods and ligand Results inspected Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0034", "assay_type": "No pharmacological activity assigned", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "resolve_missing_CCD_to_BTB; retain_buffer_role", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0034\", \"assay_type\": \"No pharmacological activity assigned\", \"ccd_id\": null, \"compound_name\": \"Bis-Tris\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results: Trimethylated lysine histone recognition; crystallization methods\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/\", \"gene\": \"DNMT3B\", \"interpretation\": \"Observed aromatic-cage occupancy by crystallization buffer. Do not classify as a validated pharmacological ligand.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"3QKJ\", \"reference_id\": \"PMID:21720545\", \"role\": \"crystallization_buffer\", \"uncertainty\": null, \"units\": null, \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "Crystallization reservoir contains 0.1 M Bis-Tris; not an affinity assay", "ccd_id": "BTB", "ccd_mapping_source": null, "chemical_id": "INCHIKEY:OWMVSZAMULFTJU-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "Bis-Tris", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:Q9UBC3:PWWP1", "endpoint_label_status": null, "evidence_class": "structural_occupancy_association", "evidence_location": "Results: Trimethylated lysine histone recognition; crystallization methods", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/", "gene": "DNMT3B", "interpretation": "Observed aromatic-cage occupancy by crystallization buffer. Do not classify as a validated pharmacological ligand.", "molecular_entity_type": "buffer_additive", "numeric_transcription_status": "not_a_quantitative_affinity_record", "numeric_value_inferred_from_structure": 0, "pH": 6.5, "pdb_id": "3QKJ", "preparation_id": "PREP:BIS-TRIS", "protein_id": "UP:Q9UBC3", "qualitative_result": null, "reference_id": "PMID:21720545", "remaining_uncertainty": "Pocket occupancy in high-concentration crystallization buffer is not evidence of potency.", "replicates": null, "role": "crystallization_buffer", "source_component_url": null, "source_id": "S07", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML; Tables 1-2, crystallization methods and ligand Results inspected", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_full_text_sections_verified" }
BPPPREP:BPPNegative / censored	DNMT3B PWWP1 Construct not curatedHomo sapiens	qualitativeBinding selectivity; assay method not verified in Figure S2qualitative binding	Source reports non-detectable affinity in the tested selectivity comparison.	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification No concentration limit, numeric Kd, assay method or construct boundaries assigned without original Supplementary Figure S2. Protein has the named PWWP domain; this qualitative observation is not an unbounded inactivity claim.	PMID:39031937 · 2024Official publisher full HTML independently inspected: Methods, Results and Figures 1–4 captions; original Supplementary Figure S2 was not retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0031", "assay_type": "Binding selectivity; assay method not verified in Figure S2", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "compound_name": "BPP", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:Q9UBC3:PWWP1", "endpoint": "qualitative", "evidence_class": "qualitative_binding", "evidence_location": "Results: selectivity paragraph referring to Figure S2", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.14981", "gene": "DNMT3B", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": true, "negative_evidence_scope": "Non-detectable binding in the source experimental comparison; assay limits not recovered.", "numeric_transcription_status": "qualitative_or_not_reported", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:BPP", "protein_id": "UP:Q9UBC3", "publication_year": 2024, "qualitative_result": "Source reports non-detectable affinity in the tested selectivity comparison.", "reference_id": "PMID:39031937", "remaining_uncertainty": "No concentration limit, numeric Kd, assay method or construct boundaries assigned without original Supplementary Figure S2. Protein has the named PWWP domain; this qualitative observation is not an unbounded inactivity claim.", "replicates": null, "retrieval_date": "2026-10-26", "role": "experimentally_studied_ligand", "source_id": "P5:S:WEI2024", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Official publisher full HTML independently inspected: Methods, Results and Figures 1\u20134 captions; original Supplementary Figure S2 was not retrieved", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
BPPPREP:BPPNegative / censored	GLYR1 PWWP1 Construct not curatedHomo sapiens	qualitativeBinding selectivity; assay method not verified in Figure S2qualitative binding	Source reports non-detectable affinity in the tested selectivity comparison.	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification No concentration limit, numeric Kd, assay method or construct boundaries assigned without original Supplementary Figure S2. Protein has the named PWWP domain; this qualitative observation is not an unbounded inactivity claim.	PMID:39031937 · 2024Official publisher full HTML independently inspected: Methods, Results and Figures 1–4 captions; original Supplementary Figure S2 was not retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0029", "assay_type": "Binding selectivity; assay method not verified in Figure S2", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "compound_name": "BPP", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:Q49A26:PWWP1", "endpoint": "qualitative", "evidence_class": "qualitative_binding", "evidence_location": "Results: selectivity paragraph referring to Figure S2", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.14981", "gene": "GLYR1", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": true, "negative_evidence_scope": "Non-detectable binding in the source experimental comparison; assay limits not recovered.", "numeric_transcription_status": "qualitative_or_not_reported", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:BPP", "protein_id": "UP:Q49A26", "publication_year": 2024, "qualitative_result": "Source reports non-detectable affinity in the tested selectivity comparison.", "reference_id": "PMID:39031937", "remaining_uncertainty": "No concentration limit, numeric Kd, assay method or construct boundaries assigned without original Supplementary Figure S2. Protein has the named PWWP domain; this qualitative observation is not an unbounded inactivity claim.", "replicates": null, "retrieval_date": "2026-10-26", "role": "experimentally_studied_ligand", "source_id": "P5:S:WEI2024", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Official publisher full HTML independently inspected: Methods, Results and Figures 1\u20134 captions; original Supplementary Figure S2 was not retrieved", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
BPPPREP:BPP	HDGFL2 PWWP1 1–93Variant: Y18SHomo sapiens	KdNMR chemical-shift perturbation Kddirect binding affinity	= 0.29 ± 0.02 mM290 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting error Temperature °C Buffer Not curated Conditions {'binding_model': '1:1 global fit of perturbed residues', 'buffer': 'HEPES-buffered saline; CSP subsection does not separately give HEPES/NaCl concentrations', 'ligand_protein_molar_ratio_range': [0, 6], 'pH': 7.4, 'protein_concentration_mM': 0.1, 'target_variant': 'Y18S'} Qualification Y18S mutant measurement; must not be pooled with wild-type affinity. Construct is the source HDGFRP2 PWWP expression fragment (1–93) carrying the stated mutation. Replicate number and titration-specific temperature are not independently specified.	PMID:39031937 · 2024Official publisher full HTML independently inspected: Methods, Results and Figures 1–4 captions; original Supplementary Figure S2 was not retrieved Original observation record { "assay_construct_end": 93, "assay_construct_start": 1, "assay_context": "purified_mutant_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0028", "assay_type": "NMR chemical-shift perturbation Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "compound_name": "BPP", "conditions": { "binding_model": "1:1 global fit of perturbed residues", "buffer": "HEPES-buffered saline; CSP subsection does not separately give HEPES/NaCl concentrations", "ligand_protein_molar_ratio_range": [ 0, 6 ], "pH": 7.4, "protein_concentration_mM": 0.1, "target_variant": "Y18S" }, "domain": "PWWP1", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 3D", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.14981", "gene": "HDGFL2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:BPP", "protein_id": "UP:Q7Z4V5", "publication_year": 2024, "reference_id": "PMID:39031937", "remaining_uncertainty": "Y18S mutant measurement; must not be pooled with wild-type affinity. Construct is the source HDGFRP2 PWWP expression fragment (1\u201393) carrying the stated mutation. Replicate number and titration-specific temperature are not independently specified.", "replicates": null, "retrieval_date": "2026-10-26", "role": "experimentally_studied_ligand", "source_id": "P5:S:WEI2024", "source_reported_uncertainty": "0.02", "source_reported_units": "mM", "source_reported_value": "0.29", "source_verification_scope": "Official publisher full HTML independently inspected: Methods, Results and Figures 1\u20134 captions; original Supplementary Figure S2 was not retrieved", "target_variant": "Y18S", "uncertainty": 0.02, "uncertainty_type": "fitting error", "uncertainty_uM": 20.0, "unit_conversion": "mM \u00d7 1000 = \u00b5M", "units": "mM", "value": 0.29, "value_operator": "=", "value_uM": 290.0, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
BPPPREP:BPP	HDGFL2 PWWP 1–93Homo sapiens	KdNMR chemical-shift perturbation Kddirect binding affinity	= 7 ± 5 µM7 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting_error Temperature — Buffer HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection Conditions {"fit_model": "1:1 binding; global fit with shared Kd", "ligand_to_protein_molar_ratio_range": [0, 6], "protein_concentration_uM": 100, "protein_construct": "HDGFL2 1–93"} Qualification Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted.	PMID:39031937 · 2024primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 93, "assay_construct_start": 1, "assay_record_id": "PWWP:ASSAY:0011", "assay_type": "NMR chemical-shift perturbation Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0011\", \"assay_type\": \"NMR chemical-shift perturbation Kd\", \"ccd_id\": \"A1D73\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"BPP\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results; Figure 1E\", \"evidence_url\": \"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981\", \"gene\": \"HDGFL2\", \"interpretation\": \"Authors report intermediate-exchange limitations and additional ITC validation. This record preserves the NMR value and fitting error.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"8Z6C\", \"reference_id\": \"PMID:39031937\", \"role\": \"experimentally_studied_fragment_ligand\", \"uncertainty\": 5, \"units\": \"\u00b5M\", \"value\": 7, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection", "ccd_id": "A1D73", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "BPP", "conditions": "{\"fit_model\": \"1:1 binding; global fit with shared Kd\", \"ligand_to_protein_molar_ratio_range\": [0, 6], \"protein_concentration_uM\": 100, \"protein_construct\": \"HDGFL2 1\u201393\"}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 1E", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981", "gene": "HDGFL2", "interpretation": "Authors report intermediate-exchange limitations and additional ITC validation. This record preserves the NMR value and fitting error.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.4, "pdb_id": "8Z6C", "preparation_id": "PREP:BPP", "protein_id": "UP:Q7Z4V5", "qualitative_result": null, "reference_id": "PMID:39031937", "remaining_uncertainty": "Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted.", "replicates": null, "role": "experimentally_studied_fragment_ligand", "source_component_url": null, "source_id": "S04", "source_reported_uncertainty": "5", "source_reported_units": "\u00b5M", "source_reported_value": "7", "source_verification_scope": "primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved", "temperature_C": null, "uncertainty": 5.0, "uncertainty_type": "fitting_error", "uncertainty_uM": 5.0, "unit_conversion": null, "units": "\u00b5M", "value": 7.0, "value_operator": "=", "value_uM": 7.0, "verification_level": "primary_full_text_sections_verified" }
NbH10PREP:NbH10	HDGFL2 PWWP1 1–93Homo sapiens	KdSPR apparent Kddirect binding affinity	= 81.2 ± 1.8 nM0.0812 µM · exact unit conversion	Experimental context Replicates 3 Uncertainty definition SD Temperature 25 °C Buffer 50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20 Conditions {"protein_construct": "HDGFL2 1–93", "replicates": 3, "uncertainty_type": "SD"} Qualification Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody–PWWP complex.	PMID:42194065 · 2026local_primary_full_text_XML_table_and_methods_verified Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 93, "assay_construct_start": 1, "assay_record_id": "PWWP11:ASSAY:0009", "assay_type": "SPR apparent Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "named_macromolecule_no_full_sequence_identity", "component_context": null, "compound_name": "NbH10", "conditions": "{\"protein_construct\": \"HDGFL2 1\u201393\", \"replicates\": 3, \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1 NbH10 column; SPR methods", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/", "gene": "HDGFL2", "interpretation": "Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody\u2013PWWP complex.", "molecular_entity_type": "nanobody", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:NbH10", "protein_id": "UP:Q7Z4V5", "qualitative_result": null, "reference_id": "PMID:42194065", "remaining_uncertainty": null, "replicates": "3", "role": "macromolecular_binder", "source_component_url": null, "source_id": "S06", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified", "temperature_C": 25, "uncertainty": 1.8, "uncertainty_type": "SD", "uncertainty_uM": 0.0018, "unit_conversion": null, "units": "nM", "value": 81.2, "value_operator": "=", "value_uM": 0.0812, "verification_level": null }
vareniclinePREP:VARENICLINE	HDGFL2 PWWP1 1–93Variant: Y18SHomo sapiens	KdNMR chemical-shift perturbation Kddirect binding affinity	= 0.78 ± 0.05 mM780 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting error Temperature °C Buffer Not curated Conditions {'binding_model': '1:1 global fit of perturbed residues', 'buffer': 'HEPES-buffered saline; CSP subsection does not separately give HEPES/NaCl concentrations', 'ligand_protein_molar_ratio_range': [0, 6], 'pH': 7.4, 'protein_concentration_mM': 0.1, 'target_variant': 'Y18S'} Qualification Y18S mutant measurement; must not be pooled with wild-type affinity. Construct is the source HDGFRP2 PWWP expression fragment (1–93) carrying the stated mutation. Replicate number and titration-specific temperature are not independently specified.	PMID:39031937 · 2024Official publisher full HTML independently inspected: Methods, Results and Figures 1–4 captions; original Supplementary Figure S2 was not retrieved Original observation record { "assay_construct_end": 93, "assay_construct_start": 1, "assay_context": "purified_mutant_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0027", "assay_type": "NMR chemical-shift perturbation Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:JQSHBVHOMNKWFT-DTORHVGOSA-N", "compound_name": "varenicline", "conditions": { "binding_model": "1:1 global fit of perturbed residues", "buffer": "HEPES-buffered saline; CSP subsection does not separately give HEPES/NaCl concentrations", "ligand_protein_molar_ratio_range": [ 0, 6 ], "pH": 7.4, "protein_concentration_mM": 0.1, "target_variant": "Y18S" }, "domain": "PWWP1", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 2E", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.14981", "gene": "HDGFL2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:VARENICLINE", "protein_id": "UP:Q7Z4V5", "publication_year": 2024, "reference_id": "PMID:39031937", "remaining_uncertainty": "Y18S mutant measurement; must not be pooled with wild-type affinity. Construct is the source HDGFRP2 PWWP expression fragment (1\u201393) carrying the stated mutation. Replicate number and titration-specific temperature are not independently specified.", "replicates": null, "retrieval_date": "2026-10-26", "role": "experimentally_studied_ligand", "source_id": "P5:S:WEI2024", "source_reported_uncertainty": "0.05", "source_reported_units": "mM", "source_reported_value": "0.78", "source_verification_scope": "Official publisher full HTML independently inspected: Methods, Results and Figures 1\u20134 captions; original Supplementary Figure S2 was not retrieved", "target_variant": "Y18S", "uncertainty": 0.05, "uncertainty_type": "fitting error", "uncertainty_uM": 50.0, "unit_conversion": "mM \u00d7 1000 = \u00b5M", "units": "mM", "value": 0.78, "value_operator": "=", "value_uM": 780.0, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
vareniclinePREP:VARENICLINE	HDGFL2 PWWP 1–93Homo sapiens	KdNMR chemical-shift perturbation Kddirect binding affinity	= 250 ± 40 µM250 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting_error Temperature — Buffer HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection Conditions {"fit_model": "1:1 binding; global fit with shared Kd", "ligand_to_protein_molar_ratio_range": [0, 6], "protein_concentration_uM": 100, "protein_construct": "HDGFL2 1–93"} Qualification Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.	PMID:39031937 · 2024primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 93, "assay_construct_start": 1, "assay_record_id": "PWWP:ASSAY:0010", "assay_type": "NMR chemical-shift perturbation Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0010\", \"assay_type\": \"NMR chemical-shift perturbation Kd\", \"ccd_id\": \"QMR\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"varenicline\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results; Figure 1E\", \"evidence_url\": \"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981\", \"gene\": \"HDGFL2\", \"interpretation\": \"Converted from 0.25 \u00b1 0.04 mM. Receptor pharmacology in other systems is not evidence of PWWP potency.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"8Z6O\", \"reference_id\": \"PMID:39031937\", \"role\": \"experimentally_studied_fragment_ligand\", \"uncertainty\": 40, \"units\": \"\u00b5M\", \"value\": 250, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection", "ccd_id": "QMR", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:JQSHBVHOMNKWFT-DTORHVGOSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "varenicline", "conditions": "{\"fit_model\": \"1:1 binding; global fit with shared Kd\", \"ligand_to_protein_molar_ratio_range\": [0, 6], \"protein_concentration_uM\": 100, \"protein_construct\": \"HDGFL2 1\u201393\"}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 1E", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981", "gene": "HDGFL2", "interpretation": "Converted from 0.25 \u00b1 0.04 mM. Receptor pharmacology in other systems is not evidence of PWWP potency.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.4, "pdb_id": "8Z6O", "preparation_id": "PREP:VARENICLINE", "protein_id": "UP:Q7Z4V5", "qualitative_result": null, "reference_id": "PMID:39031937", "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.", "replicates": null, "role": "experimentally_studied_fragment_ligand", "source_component_url": null, "source_id": "S04", "source_reported_uncertainty": "0.04", "source_reported_units": "mM", "source_reported_value": "0.25", "source_verification_scope": "primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved", "temperature_C": null, "uncertainty": 40.0, "uncertainty_type": "fitting_error", "uncertainty_uM": 40.0, "unit_conversion": "0.25 mM \u00d7 1000 = 250 \u00b5M; 0.04 mM \u00d7 1000 = 40 \u00b5M", "units": "\u00b5M", "value": 250.0, "value_operator": "=", "value_uM": 250.0, "verification_level": "primary_full_text_sections_verified" }
H4K20me3 peptide / trimethyllysine monomerPREP:H4K20me3_11mer	HDGFL2 PWWP1 Construct not curatedHomo sapiens	Qualitative / associationFluorescence polarization bindingqualitative weak binding	Very weak; too weak to fit reliably	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {"sequence": "AKRHRK(me3)VLRDN"} Qualification Solution binding cannot be quantified from this record; structural occupancy separately linked to 3QBY.	PMID:21720545 · 2011local_primary_full_text_XML_table_verified Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP11:ASSAY:0010", "assay_type": "Fluorescence polarization binding", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "defined_modified_peptide_sequence_not_monomer_identity", "component_context": null, "compound_name": "H4K20me3 peptide / trimethyllysine monomer", "conditions": "{\"sequence\": \"AKRHRK(me3)VLRDN\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "qualitative_weak_binding", "evidence_location": "Table 1 H4K20me3 / HDGF2 cell and footnote", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/", "gene": "HDGFL2", "interpretation": "Solution binding cannot be quantified from this record; structural occupancy separately linked to 3QBY.", "molecular_entity_type": "modified_histone_peptide", "numeric_transcription_status": "null_preserved_for_unfittable_binding", "numeric_value_inferred_from_structure": null, "pH": null, "pdb_id": "3QBY", "preparation_id": "PREP:H4K20me3_11mer", "protein_id": "UP:Q7Z4V5", "qualitative_result": "Very weak; too weak to fit reliably", "reference_id": "PMID:21720545", "remaining_uncertainty": null, "replicates": null, "role": "histone_peptide_assay", "source_component_url": null, "source_id": "S07", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML_table_verified", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": null }
H4K20me3 peptide / trimethyllysine monomerPREP:H4K20me3_11mer	HDGFL2 PWWP Construct not curatedHomo sapiens	Qualitative / associationX-ray structural association; no pharmacological activity assignedstructural occupancy association	No quantitative observation curated	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {} Qualification M3L has a polymer residue identity. 3QBY structural occupancy coexists with very weak unfittable solution affinity in Table 1.	PMID:21720545 · 2011local_primary_full_text_XML; Tables 1-2, crystallization methods and ligand Results inspected Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0036", "assay_type": "X-ray structural association; no pharmacological activity assigned", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "separate_whole_peptide_preparation_from_M3L_monomer", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0036\", \"assay_type\": \"X-ray structural association; no pharmacological activity assigned\", \"ccd_id\": \"M3L\", \"component_context\": \"nonstandard_polymer_monomer\", \"compound_name\": \"H4K20me3 peptide / trimethyllysine monomer\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Table 2; RCSB polymer entity 2\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/\", \"gene\": \"HDGFL2\", \"interpretation\": \"Chain H is an 11-residue H4K20me3 peptide AKRHR(M3L)VLRDN. M3L is a modified polymer monomer, not a free drug. The modeled subset must be distinguished from the full deposited peptide.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"3QBY\", \"reference_id\": \"PMID:21720545\", \"role\": \"histone methyllysine surrogate/partially resolved peptide\", \"source_component_url\": \"https://data.rcsb.org/rest/v1/core/polymer_entity/3QBY/2\", \"uncertainty\": null, \"units\": null, \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "M3L", "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "defined_modified_peptide_sequence_not_monomer_identity", "component_context": "nonstandard_polymer_monomer", "compound_name": "H4K20me3 peptide / trimethyllysine monomer", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "structural_occupancy_association", "evidence_location": "Table 2; RCSB polymer entity 2", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/", "gene": "HDGFL2", "interpretation": "Chain H is an 11-residue H4K20me3 peptide AKRHR(M3L)VLRDN. M3L is a modified polymer monomer, not a free drug. The modeled subset must be distinguished from the full deposited peptide.", "molecular_entity_type": "modified_histone_peptide", "numeric_transcription_status": "not_a_quantitative_affinity_record", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "3QBY", "preparation_id": "PREP:H4K20me3_11mer", "protein_id": "UP:Q7Z4V5", "qualitative_result": null, "reference_id": "PMID:21720545", "remaining_uncertainty": "M3L has a polymer residue identity. 3QBY structural occupancy coexists with very weak unfittable solution affinity in Table 1.", "replicates": null, "role": "histone methyllysine surrogate/partially resolved peptide", "source_component_url": "https://data.rcsb.org/rest/v1/core/polymer_entity/3QBY/2", "source_id": "S07", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML; Tables 1-2, crystallization methods and ligand Results inspected", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_full_text_sections_verified" }
BPPPREP:BPP	HDGFL3 PWWP Construct not curatedHomo sapiens	KdBinding Kd; specific assay in Figure S2 not independently inspecteddirect binding affinity	= 14 ± 2 µM14 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting_error Temperature — Buffer Not curated Conditions {} Qualification 14 ± 2 µM confirmed in primary Results; specific Figure S2 assay method remains unresolved.	PMID:39031937 · 2024primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0013", "assay_type": "Binding Kd; specific assay in Figure S2 not independently inspected", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0013\", \"assay_type\": \"Binding Kd; specific assay in Figure S2 not independently inspected\", \"ccd_id\": \"A1D73\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"BPP\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results: selectivity paragraph\", \"evidence_url\": \"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981\", \"gene\": \"HDGFL3\", \"interpretation\": \"Numerical result verified in main text; precise supporting-assay details remain to be checked.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:39031937\", \"role\": \"experimentally_studied_fragment_ligand\", \"uncertainty\": 2, \"units\": \"\u00b5M\", \"value\": 14, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "A1D73", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "BPP", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:Q9Y3E1:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results: selectivity paragraph", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981", "gene": "HDGFL3", "interpretation": "Numerical result verified in main text; precise supporting-assay details remain to be checked.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:BPP", "protein_id": "UP:Q9Y3E1", "qualitative_result": null, "reference_id": "PMID:39031937", "remaining_uncertainty": "14 \u00b1 2 \u00b5M confirmed in primary Results; specific Figure S2 assay method remains unresolved.", "replicates": null, "role": "experimentally_studied_fragment_ligand", "source_component_url": null, "source_id": "S04", "source_reported_uncertainty": "2", "source_reported_units": "\u00b5M", "source_reported_value": "14", "source_verification_scope": "primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved", "temperature_C": null, "uncertainty": 2.0, "uncertainty_type": "fitting_error", "uncertainty_uM": 2.0, "unit_conversion": null, "units": "\u00b5M", "value": 14.0, "value_operator": "=", "value_uM": 14.0, "verification_level": "primary_full_text_sections_verified" }
LLC0424PREP:LLC0424	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DC50Immunoblot degradationcellular protein degradation	= 20 nM0.02 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'RPMI-8402', 'readout': 'NSD2 protein'} Qualification Cellular whole-protein measurement. PWWP1-directed design is supported by the source, but no direct affinity is inferred and this result is not assigned to a PWWP domain.	PMID:38687638 · 2024NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0025", "assay_type": "Immunoblot degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_name": "LLC0424", "conditions": { "cell_line": "RPMI-8402", "readout": "NSD2 protein" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Abstract and Results", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11094793/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:LLC0424", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38687638", "related_domain_id": "UP:O96028:PWWP1", "remaining_uncertainty": "Cellular whole-protein measurement. PWWP1-directed design is supported by the source, but no direct affinity is inferred and this result is not assigned to a PWWP domain.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:LIU2024", "source_reported_uncertainty": null, "source_reported_units": "nM", "source_reported_value": "20", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "nM", "value": 20, "value_operator": "=", "value_uM": 0.02, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC7753PREP:UNC7753	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DC50In-cell western degradationcellular protein degradation	= 1.2 ± 0.3 µM1.2 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS', 'duration_hours': 24, 'readout': 'total NSD2 including major long/short isoforms'} Qualification Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.	PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1 Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0012", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC7753", "conditions": { "cell_line": "U2OS", "duration_hours": 24, "readout": "total NSD2 including major long/short isoforms" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Results; Figure 2A-D", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC7753", "protein_id": "UP:O96028", "publication_year": 2023, "reference_id": "PMID:36976643", "remaining_uncertainty": "Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:HANLEY2023", "source_reported_uncertainty": "0.3", "source_reported_units": "\u00b5M", "source_reported_value": "1.2", "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1", "uncertainty": 0.3, "uncertainty_type": "SEM", "uncertainty_uM": 0.3, "units": "\u00b5M", "value": 1.2, "value_operator": "=", "value_uM": 1.2, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8153PREP:UNC8153	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DC50In-cell western degradationcellular protein degradation	= 0.35 ± 0.13 µM0.35 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS', 'duration_hours': 24, 'readout': 'total NSD2 including major long/short isoforms'} Qualification Original numerical values and uncertainties are corroborated by the explicitly reused row of Nie 2024 Table 1. Original Figure 3 has multiple statistic/replicate annotations; its numeric panel was not independently opened, so the relevant annotation is left uncurated. No independent 2024 replicate is counted.	PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1 Original observation record { "additional_source_urls": [ "https://www.nature.com/articles/s41589-024-01660-y/tables/1" ], "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0010", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:MDIKJIFCQAJLPD-UHFFFAOYSA-N", "compound_name": "UNC8153", "conditions": { "cell_line": "U2OS", "duration_hours": 24, "readout": "total NSD2 including major long/short isoforms" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Results; Figure 3A; reused-data row Nie 2024 Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8153", "protein_id": "UP:O96028", "publication_year": 2023, "reference_id": "PMID:36976643", "remaining_uncertainty": "Original numerical values and uncertainties are corroborated by the explicitly reused row of Nie 2024 Table 1. Original Figure 3 has multiple statistic/replicate annotations; its numeric panel was not independently opened, so the relevant annotation is left uncurated. No independent 2024 replicate is counted.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:HANLEY2023", "source_reported_uncertainty": "0.13", "source_reported_units": "\u00b5M", "source_reported_value": "0.35", "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1", "uncertainty": 0.13, "uncertainty_type": null, "uncertainty_uM": 0.13, "underlying_measurement_id": "HANLEY2023:UNC8153:ICW:DC50", "units": "\u00b5M", "value": 0.35, "value_operator": "=", "value_uM": 0.35, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8269PREP:UNC8269	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DC50In-cell western degradationcellular protein degradation	= 1.01 ± 0.37 µM1.01 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS'} Qualification Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0017", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8269", "conditions": { "cell_line": "U2OS" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Table 1; Supplementary Figure 2", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8269", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "0.37", "source_reported_units": "\u00b5M", "source_reported_value": "1.01", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 0.37, "uncertainty_type": "SD", "uncertainty_uM": 0.37, "units": "\u00b5M", "value": 1.01, "value_operator": "=", "value_uM": 1.01, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8325PREP:UNC8325	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DC50In-cell western degradationcellular protein degradation	= 0.71 ± 0.25 µM0.71 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS'} Qualification Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0020", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8325", "conditions": { "cell_line": "U2OS" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Table 1; Supplementary Figure 2", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8325", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "0.25", "source_reported_units": "\u00b5M", "source_reported_value": "0.71", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 0.25, "uncertainty_type": "SD", "uncertainty_uM": 0.25, "units": "\u00b5M", "value": 0.71, "value_operator": "=", "value_uM": 0.71, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8732PREP:UNC8732	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DC50In-cell western degradationcellular protein degradation	= 0.06 ± 0.03 µM0.06 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS'} Qualification Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0023", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:CSTHTJLTORVUST-UHFFFAOYSA-N", "compound_name": "UNC8732", "conditions": { "cell_line": "U2OS" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Table 1; Supplementary Figure 2", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8732", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "0.03", "source_reported_units": "\u00b5M", "source_reported_value": "0.06", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 0.03, "uncertainty_type": "SD", "uncertainty_uM": 0.03, "units": "\u00b5M", "value": 0.06, "value_operator": "=", "value_uM": 0.06, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
LLC0424PREP:LLC0424	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DmaxImmunoblot degradationcellular protein degradation	= 96 percent	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'RPMI-8402', 'readout': 'NSD2 protein'} Qualification Cellular whole-protein measurement. No PWWP-domain affinity inferred; replicate/uncertainty/duration not populated without assay-specific verification.	PMID:38687638 · 2024NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0026", "assay_type": "Immunoblot degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_name": "LLC0424", "conditions": { "cell_line": "RPMI-8402", "readout": "NSD2 protein" }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Abstract and Results", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11094793/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:LLC0424", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38687638", "related_domain_id": "UP:O96028:PWWP1", "remaining_uncertainty": "Cellular whole-protein measurement. No PWWP-domain affinity inferred; replicate/uncertainty/duration not populated without assay-specific verification.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:LIU2024", "source_reported_uncertainty": null, "source_reported_units": "percent", "source_reported_value": "96", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "percent", "value": 96, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC7753PREP:UNC7753	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DmaxIn-cell western degradationcellular protein degradation	= 81 percent	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS', 'duration_hours': 24} Qualification Main-text 81% transcribed; original numeric figure uncertainty not separately verified, so no uncertainty filled.	PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1 Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0013", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC7753", "conditions": { "cell_line": "U2OS", "duration_hours": 24 }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Results; Figure 2A-D", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC7753", "protein_id": "UP:O96028", "publication_year": 2023, "reference_id": "PMID:36976643", "remaining_uncertainty": "Main-text 81% transcribed; original numeric figure uncertainty not separately verified, so no uncertainty filled.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:HANLEY2023", "source_reported_uncertainty": null, "source_reported_units": "percent", "source_reported_value": "81", "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "percent", "value": 81, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8153PREP:UNC8153	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DmaxIn-cell western degradationcellular protein degradation	= 79 ± 1 percent	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS', 'duration_hours': 24, 'readout': 'total NSD2 including major long/short isoforms'} Qualification Original numerical values and uncertainties are corroborated by the explicitly reused row of Nie 2024 Table 1. Original Figure 3 has multiple statistic/replicate annotations; its numeric panel was not independently opened, so the relevant annotation is left uncurated. No independent 2024 replicate is counted.	PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1 Original observation record { "additional_source_urls": [ "https://www.nature.com/articles/s41589-024-01660-y/tables/1" ], "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0011", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:MDIKJIFCQAJLPD-UHFFFAOYSA-N", "compound_name": "UNC8153", "conditions": { "cell_line": "U2OS", "duration_hours": 24, "readout": "total NSD2 including major long/short isoforms" }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Figure 3A; reused-data row Nie 2024 Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8153", "protein_id": "UP:O96028", "publication_year": 2023, "reference_id": "PMID:36976643", "remaining_uncertainty": "Original numerical values and uncertainties are corroborated by the explicitly reused row of Nie 2024 Table 1. Original Figure 3 has multiple statistic/replicate annotations; its numeric panel was not independently opened, so the relevant annotation is left uncurated. No independent 2024 replicate is counted.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:HANLEY2023", "source_reported_uncertainty": "1", "source_reported_units": "percent", "source_reported_value": "79", "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1", "uncertainty": 1, "uncertainty_type": null, "uncertainty_uM": null, "underlying_measurement_id": "HANLEY2023:UNC8153:ICW:Dmax", "units": "percent", "value": 79, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8269PREP:UNC8269	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DmaxIn-cell western degradationcellular protein degradation	= 59 ± 6 percent	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS'} Qualification Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0016", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8269", "conditions": { "cell_line": "U2OS" }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Table 1; Supplementary Figure 2", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8269", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "6", "source_reported_units": "percent", "source_reported_value": "59", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 6, "uncertainty_type": "SD", "uncertainty_uM": null, "units": "percent", "value": 59, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8325PREP:UNC8325	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DmaxIn-cell western degradationcellular protein degradation	= 71 ± 3 percent	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS'} Qualification Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0019", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8325", "conditions": { "cell_line": "U2OS" }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Table 1; Supplementary Figure 2", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8325", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "3", "source_reported_units": "percent", "source_reported_value": "71", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 3, "uncertainty_type": "SD", "uncertainty_uM": null, "units": "percent", "value": 71, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8732PREP:UNC8732	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DmaxIn-cell western degradationcellular protein degradation	= 97 ± 2 percent	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS'} Qualification Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0022", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:CSTHTJLTORVUST-UHFFFAOYSA-N", "compound_name": "UNC8732", "conditions": { "cell_line": "U2OS" }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Table 1; Supplementary Figure 2", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8732", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "2", "source_reported_units": "percent", "source_reported_value": "97", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 2, "uncertainty_type": "SD", "uncertainty_uM": null, "units": "percent", "value": 97, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC6934PREP:UNC6934	NSD2 PWWP1 208–368Homo sapiens	IC50AlphaScreen IC50biochemical interaction inhibition	= 104 ± 13 nM0.104 µM · exact unit conversion	Experimental context Replicates 3 independent experiments; each 10-point curve in duplicate Uncertainty definition Not curated; do not assume SD or SEM Temperature 23 °C Buffer 150 mM NaCl; 20 mM Tris; 0.01% BSA; 0.01% NP-40; 1 mM DTT (DTT omitted from bead buffer) Conditions {"phase3_verification": "SV02A", "protein_construct": "NSD2 208–368", "readout": "His-NSD2-PWWP1 binding to H3K36me2 designer nucleosome", "tag": "6His", "temperature_scope": "23°C compound/protein and nucleosome preincubation; bead incubation room temperature"} Qualification 23°C is the antagonist preincubation temperature, not a verified readout temperature. Final DMSO fraction and SD-versus-SEM definition for ±13 nM are not explicit in the inspected assay-specific text.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0005", "assay_type": "AlphaScreen IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0005\", \"assay_type\": \"AlphaScreen IC50\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Discussion; biochemical characterization\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"Binding-interaction assay, not a direct catalytic SET-domain inhibition measurement.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6XCG\", \"reference_id\": \"PMID:34782742\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 13, \"units\": \"nM\", \"value\": 104, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "150 mM NaCl; 20 mM Tris; 0.01% BSA; 0.01% NP-40; 1 mM DTT (DTT omitted from bead buffer)", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"phase3_verification\": \"SV02A\", \"protein_construct\": \"NSD2 208\u2013368\", \"readout\": \"His-NSD2-PWWP1 binding to H3K36me2 designer nucleosome\", \"tag\": \"6His\", \"temperature_scope\": \"23\u00b0C compound/protein and nucleosome preincubation; bead incubation room temperature\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Discussion; biochemical characterization", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": "Binding-interaction assay, not a direct catalytic SET-domain inhibition measurement.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "6XCG", "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "23\u00b0C is the antagonist preincubation temperature, not a verified readout temperature. Final DMSO fraction and SD-versus-SEM definition for \u00b113 nM are not explicit in the inspected assay-specific text.", "replicates": "3 independent experiments; each 10-point curve in duplicate", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": "13", "source_reported_units": "nM", "source_reported_value": "104", "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": 23, "uncertainty": 13.0, "uncertainty_type": null, "uncertainty_uM": 0.013, "unit_conversion": null, "units": "nM", "value": 104.0, "value_operator": "=", "value_uM": 0.104, "verification_level": "primary_full_text_sections_verified" }
compound 12 (Collie 2025)PREP:COLLIE2025_12	NSD2 PWWP1 208–368Homo sapiens	IC50HTRF displacement IC50biochemical interaction inhibition	= 2.49 ± 0.06 µM2.49 µM · exact unit conversion	Experimental context Replicates 2 Uncertainty definition SEM Temperature — Buffer Not curated Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": 2, "uncertainty_type": "SEM"} Qualification HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts. Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0030", "assay_type": "HTRF displacement IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0030\", \"assay_type\": \"HTRF displacement IC50\", \"ccd_id\": null, \"compound_name\": \"compound 12 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": 2, \"uncertainty_type\": \"SEM\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.06, \"units\": \"\u00b5M\", \"value\": 2.49, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 12 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": 2, \"uncertainty_type\": \"SEM\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_12", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.", "replicates": "2", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.06", "source_reported_units": "\u00b5M", "source_reported_value": "2.49", "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.", "temperature_C": null, "uncertainty": 0.06, "uncertainty_type": "SEM", "uncertainty_uM": 0.06, "unit_conversion": null, "units": "\u00b5M", "value": 2.49, "value_operator": "=", "value_uM": 2.49, "verification_level": "primary_full_text_sections_verified" }
compound 13 (Collie 2025)PREP:COLLIE2025_13	NSD2 PWWP1 208–368Homo sapiens	IC50HTRF displacement IC50biochemical interaction inhibition	= 1.31 ± 0.06 µM1.31 µM · exact unit conversion	Experimental context Replicates at least 3 Uncertainty definition SEM Temperature — Buffer Not curated Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SEM"} Qualification HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts. Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0031", "assay_type": "HTRF displacement IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0031\", \"assay_type\": \"HTRF displacement IC50\", \"ccd_id\": null, \"compound_name\": \"compound 13 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SEM\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.06, \"units\": \"\u00b5M\", \"value\": 1.31, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 13 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SEM\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_13", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.", "replicates": "at least 3", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.06", "source_reported_units": "\u00b5M", "source_reported_value": "1.31", "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.", "temperature_C": null, "uncertainty": 0.06, "uncertainty_type": "SEM", "uncertainty_uM": 0.06, "unit_conversion": null, "units": "\u00b5M", "value": 1.31, "value_operator": "=", "value_uM": 1.31, "verification_level": "primary_full_text_sections_verified" }
compound 6 (Collie 2025)PREP:COLLIE2025_6	NSD2 PWWP1 208–368Homo sapiens	IC50HTRF displacement IC50biochemical interaction inhibition	= 1.24 ± 0.08 µM1.24 µM · exact unit conversion	Experimental context Replicates 2 Uncertainty definition SEM Temperature — Buffer Not curated Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": 2, "uncertainty_type": "SEM"} Qualification HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts. Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0028", "assay_type": "HTRF displacement IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0028\", \"assay_type\": \"HTRF displacement IC50\", \"ccd_id\": null, \"compound_name\": \"compound 6 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": 2, \"uncertainty_type\": \"SEM\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.08, \"units\": \"\u00b5M\", \"value\": 1.24, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 6 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": 2, \"uncertainty_type\": \"SEM\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_6", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.", "replicates": "2", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.08", "source_reported_units": "\u00b5M", "source_reported_value": "1.24", "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.", "temperature_C": null, "uncertainty": 0.08, "uncertainty_type": "SEM", "uncertainty_uM": 0.08, "unit_conversion": null, "units": "\u00b5M", "value": 1.24, "value_operator": "=", "value_uM": 1.24, "verification_level": "primary_full_text_sections_verified" }
compound 7 (Collie 2025)PREP:COLLIE2025_7	NSD2 PWWP1 208–368Homo sapiens	IC50HTRF displacement IC50biochemical interaction inhibition	= 0.21 ± 0.01 µM0.21 µM · exact unit conversion	Experimental context Replicates at least 3 Uncertainty definition SEM Temperature — Buffer Not curated Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SEM"} Qualification HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts. Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0029", "assay_type": "HTRF displacement IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0029\", \"assay_type\": \"HTRF displacement IC50\", \"ccd_id\": \"A1IEF\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"compound 7 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SEM\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOE\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.01, \"units\": \"\u00b5M\", \"value\": 0.21, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "A1IEF", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "compound 7 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SEM\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "9FOE", "preparation_id": "PREP:COLLIE2025_7", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.", "replicates": "at least 3", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.01", "source_reported_units": "\u00b5M", "source_reported_value": "0.21", "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.", "temperature_C": null, "uncertainty": 0.01, "uncertainty_type": "SEM", "uncertainty_uM": 0.01, "unit_conversion": null, "units": "\u00b5M", "value": 0.21, "value_operator": "=", "value_uM": 0.21, "verification_level": "primary_full_text_sections_verified" }
MR837 (3f)PREP:MR837	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 7 ± 3 µM7 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {} Qualification Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.	PMID:33522809 · 2021primary_article_selected_indexed_full_text_passages; original figures and supplement not independently opened Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0001", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0001\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"Q5D\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"MR837 (3f)\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results; Figure 2\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/\", \"gene\": \"NSD2\", \"interpretation\": \"Distinct condition from the lower-DMSO SPR measurement; do not average.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6UE6\", \"reference_id\": \"PMID:33522809\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 3, \"units\": \"\u00b5M\", \"value\": 7, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "Q5D", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:WYAGGMZTARRIDX-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "MR837 (3f)", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 2", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "interpretation": "Distinct condition from the lower-DMSO SPR measurement; do not average.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "6UE6", "preparation_id": "PREP:MR837", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:33522809", "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.", "replicates": null, "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S01", "source_reported_uncertainty": "3", "source_reported_units": "\u00b5M", "source_reported_value": "7", "source_verification_scope": "primary_article_selected_indexed_full_text_passages; original figures and supplement not independently opened", "temperature_C": null, "uncertainty": 3.0, "uncertainty_type": null, "uncertainty_uM": 3.0, "unit_conversion": null, "units": "\u00b5M", "value": 7.0, "value_operator": "=", "value_uM": 7.0, "verification_level": "primary_full_text_sections_verified" }
MR837 (3f)PREP:MR837	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 3.4 ± 0.4 µM3.4 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {"DMSO_percent": 0.5} Qualification Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.	PMID:33522809 · 2021primary_article_selected_indexed_full_text_passages; original figures and supplement not independently opened Original observation record { "DMSO_percent": 0.5, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0002", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0002\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"Q5D\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"MR837 (3f)\", \"conditions\": {\"DMSO_percent\": 0.5}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results; Figure S6\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/\", \"gene\": \"NSD2\", \"interpretation\": \"Lower-DMSO measurement; preserve assay conditions.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6UE6\", \"reference_id\": \"PMID:33522809\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.4, \"units\": \"\u00b5M\", \"value\": 3.4, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "Q5D", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:WYAGGMZTARRIDX-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "MR837 (3f)", "conditions": "{\"DMSO_percent\": 0.5}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure S6", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "interpretation": "Lower-DMSO measurement; preserve assay conditions.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "6UE6", "preparation_id": "PREP:MR837", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:33522809", "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.", "replicates": null, "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S01", "source_reported_uncertainty": "0.4", "source_reported_units": "\u00b5M", "source_reported_value": "3.4", "source_verification_scope": "primary_article_selected_indexed_full_text_passages; original figures and supplement not independently opened", "temperature_C": null, "uncertainty": 0.4, "uncertainty_type": null, "uncertainty_uM": 0.4, "unit_conversion": null, "units": "\u00b5M", "value": 3.4, "value_operator": "=", "value_uM": 3.4, "verification_level": "primary_full_text_sections_verified" }
MRT866PREP:MRT866	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 349 ± 19 nM0.349 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {} Qualification 349 ± 19 nM verified in Results; MRT866-specific SPR construct/protocol, replicate definition and uncertainty statistic remain unresolved. Crystallography 211–350 is not assigned as the binding-assay construct.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0003", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0003\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"Y6V\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"MRT866\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results: Discovery of a potent ligand targeting NSD2-PWWP1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"7MDN\", \"reference_id\": \"PMID:34782742\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 19, \"units\": \"nM\", \"value\": 349, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "Y6V", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:XPHOPAPMJBSLFN-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "MRT866", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results: Discovery of a potent ligand targeting NSD2-PWWP1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": null, "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "7MDN", "preparation_id": "PREP:MRT866", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "349 \u00b1 19 nM verified in Results; MRT866-specific SPR construct/protocol, replicate definition and uncertainty statistic remain unresolved. Crystallography 211\u2013350 is not assigned as the binding-assay construct.", "replicates": null, "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": "19", "source_reported_units": "nM", "source_reported_value": "349", "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": null, "uncertainty": 19.0, "uncertainty_type": null, "uncertainty_uM": 0.019, "unit_conversion": null, "units": "nM", "value": 349.0, "value_operator": "=", "value_uM": 0.349, "verification_level": "primary_full_text_sections_verified" }
UNC6934PREP:UNC6934	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 91 ± 8 nM0.091 µM · exact unit conversion	Experimental context Replicates 3 (triplicate experiments; independence level not specified) Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO Conditions {"phase3_verification": "SV02A", "protein_construct": "NSD2 208–368", "purified_tags": "AviTag/NSD2(208–368)/polyhistidine; biotinylated", "source_protocol_highest_concentration_uM": 2} Qualification SPR temperature and exact uncertainty definition are not specified in the inspected assay-specific source. Triplicate experiments reported. Preparation/assay construct differs from crystallization 211–350.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": 0.5, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0004", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0004\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results; Figures 1\u20132\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6XCG\", \"reference_id\": \"PMID:34782742\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 8, \"units\": \"nM\", \"value\": 91, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"phase3_verification\": \"SV02A\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"AviTag/NSD2(208\u2013368)/polyhistidine; biotinylated\", \"source_protocol_highest_concentration_uM\": 2}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figures 1\u20132", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": null, "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.4, "pdb_id": "6XCG", "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "SPR temperature and exact uncertainty definition are not specified in the inspected assay-specific source. Triplicate experiments reported. Preparation/assay construct differs from crystallization 211\u2013350.", "replicates": "3 (triplicate experiments; independence level not specified)", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": "8", "source_reported_units": "nM", "source_reported_value": "91", "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": null, "uncertainty": 8.0, "uncertainty_type": null, "uncertainty_uM": 0.008, "unit_conversion": null, "units": "nM", "value": 91.0, "value_operator": "=", "value_uM": 0.091, "verification_level": "primary_full_text_sections_verified" }
UNC6934PREP:UNC6934	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.24 ± 0.03 µM0.24 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0014", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0014\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 0.03, \"units\": \"\u00b5M\", \"value\": 0.24, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.03", "source_reported_units": "\u00b5M", "source_reported_value": "0.24", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.03, "uncertainty_type": "SD", "uncertainty_uM": 0.03, "unit_conversion": null, "units": "\u00b5M", "value": 0.24, "value_operator": "=", "value_uM": 0.24, "verification_level": "primary_full_text_sections_verified" }
UNC6934PREP:UNC6934	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 1.33 ± 0.19 µM1.33 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0021", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0021\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 0.19, \"units\": \"\u00b5M\", \"value\": 1.33, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.19", "source_reported_units": "\u00b5M", "source_reported_value": "1.33", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.19, "uncertainty_type": "SD", "uncertainty_uM": 0.19, "unit_conversion": null, "units": "\u00b5M", "value": 1.33, "value_operator": "=", "value_uM": 1.33, "verification_level": "primary_full_text_sections_verified" }
UNC7096PREP:UNC7096	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 46 nM0.046 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification UNC7096-specific construct, buffer, uncertainty and replicate definition were not independently verified; the general UNC6934/UNC7145 SPR protocol is not automatically transferred.	PMID:34782742 · 2022NCBI BioC primary full text; UNC7096 Results and UNC7145 chemical transformation; primary Methods/captions inspected Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0007", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:JXTDBIVBSAFNBJ-PLCURSASSA-N", "compound_name": "UNC7096", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results: Development of selective affinity reagents; Extended Data Figure 6", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC7096", "protein_id": "UP:O96028", "publication_year": 2022, "reference_id": "PMID:34782742", "remaining_uncertainty": "UNC7096-specific construct, buffer, uncertainty and replicate definition were not independently verified; the general UNC6934/UNC7145 SPR protocol is not automatically transferred.", "replicates": null, "retrieval_date": "2026-10-26", "role": "affinity_reagent", "source_id": "P5:S:DILWORTH2021", "source_reported_uncertainty": null, "source_reported_units": "nM", "source_reported_value": "46", "source_verification_scope": "NCBI BioC primary full text; UNC7096 Results and UNC7145 chemical transformation; primary Methods/captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "nM", "value": 46, "value_operator": "=", "value_uM": 0.046, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8153PREP:UNC8153	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 24 ± 7 nM0.024 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification Original value and uncertainty preserved from later primary-table reprint; original Figure 3 numeric image not separately opened. Original experiment replicate/statistic assignment and construct remain uncurated; this is not a second 2024 experiment.	PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1 Original observation record { "additional_source_urls": [ "https://www.nature.com/articles/s41589-024-01660-y/tables/1" ], "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0009", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:MDIKJIFCQAJLPD-UHFFFAOYSA-N", "compound_name": "UNC8153", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Figure 3A; numeric value visually verified in asterisk-marked original-data row of Nie 2024 Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8153", "protein_id": "UP:O96028", "publication_year": 2023, "reference_id": "PMID:36976643", "remaining_uncertainty": "Original value and uncertainty preserved from later primary-table reprint; original Figure 3 numeric image not separately opened. Original experiment replicate/statistic assignment and construct remain uncurated; this is not a second 2024 experiment.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:HANLEY2023", "source_reported_uncertainty": "7", "source_reported_units": "nM", "source_reported_value": "24", "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1", "uncertainty": 7, "uncertainty_type": null, "uncertainty_uM": 0.007, "underlying_measurement_id": "HANLEY2023:UNC8153:SPR", "units": "nM", "value": 24, "value_operator": "=", "value_uM": 0.024, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8269PREP:UNC8269	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 36 ± 21 nM0.036 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions Not curated Qualification Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0015", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8269", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1; Supplementary Figure 1", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8269", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "21", "source_reported_units": "nM", "source_reported_value": "36", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 21, "uncertainty_type": "SD", "uncertainty_uM": 0.021, "units": "nM", "value": 36, "value_operator": "=", "value_uM": 0.036, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8325PREP:UNC8325	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 41 ± 19 nM0.041 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions Not curated Qualification Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0018", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8325", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1; Supplementary Figure 1", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8325", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "19", "source_reported_units": "nM", "source_reported_value": "41", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 19, "uncertainty_type": "SD", "uncertainty_uM": 0.019, "units": "nM", "value": 41, "value_operator": "=", "value_uM": 0.041, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8732PREP:UNC8732	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 76 ± 4 nM0.076 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions Not curated Qualification Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0021", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:CSTHTJLTORVUST-UHFFFAOYSA-N", "compound_name": "UNC8732", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1; Supplementary Figure 1", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8732", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "4", "source_reported_units": "nM", "source_reported_value": "76", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 4, "uncertainty_type": "SD", "uncertainty_uM": 0.004, "units": "nM", "value": 76, "value_operator": "=", "value_uM": 0.076, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
compound 1 (de Freitas 2021)PREP:DEFREITAS2021_1	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 41 ± 8 µM41 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification Source text directly reports preparation and Kd ± uncertainty; supplementary figure and compound-1-specific buffer/construct/statistic not independently recovered. The SPR Methods paragraph explicitly names 3f, so its conditions are not copied to compound 1.	PMID:33522809 · 2021NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0033", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "compound 1 (de Freitas 2021)", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure S1a", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:DEFREITAS2021_1", "protein_id": "UP:O96028", "publication_year": 2021, "reference_id": "PMID:33522809", "remaining_uncertainty": "Source text directly reports preparation and Kd \u00b1 uncertainty; supplementary figure and compound-1-specific buffer/construct/statistic not independently recovered. The SPR Methods paragraph explicitly names 3f, so its conditions are not copied to compound 1.", "replicates": null, "retrieval_date": "2026-10-26", "role": "fragment_hit", "source_id": "P5:S:DEFREITAS2021", "source_reported_uncertainty": "8", "source_reported_units": "\u00b5M", "source_reported_value": "41", "source_verification_scope": "NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved", "uncertainty": 8, "uncertainty_type": null, "uncertainty_uM": 8, "units": "\u00b5M", "value": 41, "value_operator": "=", "value_uM": 41, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
compound 1 (de Freitas 2021)PREP:DEFREITAS2021_1	NSD2 PWWP1 Construct not curatedHomo sapiens	KdReverse ITC Kddirect binding affinity	= 8.9 µM8.9 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'compound_cell_concentration_uM': 40, 'protein_syringe_concentration_mM': 1, 'reverse_titration_reason': 'Limited compound solubility', 'titration_direction': 'Protein into compound'} Qualification No uncertainty, replicate count or exact stereochemical assignment inferred from the primary Results value. A distinct reverse-ITC experiment is retained separately from SPR.	PMID:33522809 · 2021NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0034", "assay_type": "Reverse ITC Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "compound 1 (de Freitas 2021)", "conditions": { "compound_cell_concentration_uM": 40, "protein_syringe_concentration_mM": 1, "reverse_titration_reason": "Limited compound solubility", "titration_direction": "Protein into compound" }, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure S1b", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:DEFREITAS2021_1", "protein_id": "UP:O96028", "publication_year": 2021, "reference_id": "PMID:33522809", "remaining_uncertainty": "No uncertainty, replicate count or exact stereochemical assignment inferred from the primary Results value. A distinct reverse-ITC experiment is retained separately from SPR.", "replicates": null, "retrieval_date": "2026-10-26", "role": "fragment_hit", "source_id": "P5:S:DEFREITAS2021", "source_reported_uncertainty": null, "source_reported_units": "\u00b5M", "source_reported_value": "8.9", "source_verification_scope": "NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "\u00b5M", "value": 8.9, "value_operator": "=", "value_uM": 8.9, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
compound 11 (Collie 2025)PREP:COLLIE2025_11	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 1.02 ± 0.19 µM1.02 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0018", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0018\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 11 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"crystallographic_component_ids\": [\"A1IFS\", \"A1IEG\"], \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded. Compound 11 is the assayed preparation; 9FOC models two stereoisomer CCDs. Do not assign the aggregate affinity separately to each pure stereoisomer.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOC\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.19, \"units\": \"\u00b5M\", \"value\": 1.02, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 11 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": "[\"A1IFS\", \"A1IEG\"]", "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded. Compound 11 is the assayed preparation; 9FOC models two stereoisomer CCDs. Do not assign the aggregate affinity separately to each pure stereoisomer.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "9FOC", "preparation_id": "PREP:COLLIE2025_11", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.19", "source_reported_units": "\u00b5M", "source_reported_value": "1.02", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.19, "uncertainty_type": "SD", "uncertainty_uM": 0.19, "unit_conversion": null, "units": "\u00b5M", "value": 1.02, "value_operator": "=", "value_uM": 1.02, "verification_level": "primary_full_text_sections_verified" }
compound 11 (Collie 2025)PREP:COLLIE2025_11	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 2.3 ± 0.31 µM2.3 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0025", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0025\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 11 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"crystallographic_component_ids\": [\"A1IFS\", \"A1IEG\"], \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded. Compound 11 is the assayed preparation; 9FOC models two stereoisomer CCDs. Do not assign the aggregate affinity separately to each pure stereoisomer.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOC\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.31, \"units\": \"\u00b5M\", \"value\": 2.3, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 11 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": "[\"A1IFS\", \"A1IEG\"]", "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded. Compound 11 is the assayed preparation; 9FOC models two stereoisomer CCDs. Do not assign the aggregate affinity separately to each pure stereoisomer.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "9FOC", "preparation_id": "PREP:COLLIE2025_11", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.31", "source_reported_units": "\u00b5M", "source_reported_value": "2.3", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.31, "uncertainty_type": "SD", "uncertainty_uM": 0.31, "unit_conversion": null, "units": "\u00b5M", "value": 2.3, "value_operator": "=", "value_uM": 2.3, "verification_level": "primary_full_text_sections_verified" }
compound 12 (Collie 2025)PREP:COLLIE2025_12	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 1.47 ± 0.13 µM1.47 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0019", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0019\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 12 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.13, \"units\": \"\u00b5M\", \"value\": 1.47, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 12 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_12", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.13", "source_reported_units": "\u00b5M", "source_reported_value": "1.47", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.13, "uncertainty_type": "SD", "uncertainty_uM": 0.13, "unit_conversion": null, "units": "\u00b5M", "value": 1.47, "value_operator": "=", "value_uM": 1.47, "verification_level": "primary_full_text_sections_verified" }
compound 12 (Collie 2025)PREP:COLLIE2025_12	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 2.8 ± 0.31 µM2.8 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0026", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0026\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 12 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.31, \"units\": \"\u00b5M\", \"value\": 2.8, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 12 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_12", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.31", "source_reported_units": "\u00b5M", "source_reported_value": "2.8", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.31, "uncertainty_type": "SD", "uncertainty_uM": 0.31, "unit_conversion": null, "units": "\u00b5M", "value": 2.8, "value_operator": "=", "value_uM": 2.8, "verification_level": "primary_full_text_sections_verified" }
compound 13 (Collie 2025)PREP:COLLIE2025_13	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.75 ± 0.04 µM0.75 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0020", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0020\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 13 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.04, \"units\": \"\u00b5M\", \"value\": 0.75, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 13 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_13", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.04", "source_reported_units": "\u00b5M", "source_reported_value": "0.75", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.04, "uncertainty_type": "SD", "uncertainty_uM": 0.04, "unit_conversion": null, "units": "\u00b5M", "value": 0.75, "value_operator": "=", "value_uM": 0.75, "verification_level": "primary_full_text_sections_verified" }
compound 13 (Collie 2025)PREP:COLLIE2025_13	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 1.52 ± 0.05 µM1.52 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0027", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0027\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 13 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.05, \"units\": \"\u00b5M\", \"value\": 1.52, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 13 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_13", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.05", "source_reported_units": "\u00b5M", "source_reported_value": "1.52", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.05, "uncertainty_type": "SD", "uncertainty_uM": 0.05, "unit_conversion": null, "units": "\u00b5M", "value": 1.52, "value_operator": "=", "value_uM": 1.52, "verification_level": "primary_full_text_sections_verified" }
compound 5 (Collie 2025)PREP:COLLIE2025_5	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.16 ± 0.03 µM0.16 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0015", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0015\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 5 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.03, \"units\": \"\u00b5M\", \"value\": 0.16, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 5 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_5", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.03", "source_reported_units": "\u00b5M", "source_reported_value": "0.16", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.03, "uncertainty_type": "SD", "uncertainty_uM": 0.03, "unit_conversion": null, "units": "\u00b5M", "value": 0.16, "value_operator": "=", "value_uM": 0.16, "verification_level": "primary_full_text_sections_verified" }
compound 5 (Collie 2025)PREP:COLLIE2025_5	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 0.92 ± 0.03 µM0.92 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0022", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0022\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 5 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.03, \"units\": \"\u00b5M\", \"value\": 0.92, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 5 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_5", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.03", "source_reported_units": "\u00b5M", "source_reported_value": "0.92", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.03, "uncertainty_type": "SD", "uncertainty_uM": 0.03, "unit_conversion": null, "units": "\u00b5M", "value": 0.92, "value_operator": "=", "value_uM": 0.92, "verification_level": "primary_full_text_sections_verified" }
compound 6 (Collie 2025)PREP:COLLIE2025_6	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.71 ± 0.09 µM0.71 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0016", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0016\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 6 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.09, \"units\": \"\u00b5M\", \"value\": 0.71, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 6 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_6", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.09", "source_reported_units": "\u00b5M", "source_reported_value": "0.71", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.09, "uncertainty_type": "SD", "uncertainty_uM": 0.09, "unit_conversion": null, "units": "\u00b5M", "value": 0.71, "value_operator": "=", "value_uM": 0.71, "verification_level": "primary_full_text_sections_verified" }
compound 6 (Collie 2025)PREP:COLLIE2025_6	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 5.83 ± 0.64 µM5.83 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0023", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0023\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 6 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.64, \"units\": \"\u00b5M\", \"value\": 5.83, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 6 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_6", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.64", "source_reported_units": "\u00b5M", "source_reported_value": "5.83", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.64, "uncertainty_type": "SD", "uncertainty_uM": 0.64, "unit_conversion": null, "units": "\u00b5M", "value": 5.83, "value_operator": "=", "value_uM": 5.83, "verification_level": "primary_full_text_sections_verified" }
compound 7 (Collie 2025)PREP:COLLIE2025_7	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.15 ± 0.02 µM0.15 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0017", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0017\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"A1IEF\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"compound 7 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOE\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.02, \"units\": \"\u00b5M\", \"value\": 0.15, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "A1IEF", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "compound 7 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "9FOE", "preparation_id": "PREP:COLLIE2025_7", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.02", "source_reported_units": "\u00b5M", "source_reported_value": "0.15", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.02, "uncertainty_type": "SD", "uncertainty_uM": 0.02, "unit_conversion": null, "units": "\u00b5M", "value": 0.15, "value_operator": "=", "value_uM": 0.15, "verification_level": "primary_full_text_sections_verified" }
compound 7 (Collie 2025)PREP:COLLIE2025_7	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 0.76 ± 0.01 µM0.76 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0024", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0024\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"A1IEF\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"compound 7 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOE\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.01, \"units\": \"\u00b5M\", \"value\": 0.76, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "A1IEF", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "compound 7 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "9FOE", "preparation_id": "PREP:COLLIE2025_7", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.01", "source_reported_units": "\u00b5M", "source_reported_value": "0.76", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.01, "uncertainty_type": "SD", "uncertainty_uM": 0.01, "unit_conversion": null, "units": "\u00b5M", "value": 0.76, "value_operator": "=", "value_uM": 0.76, "verification_level": "primary_full_text_sections_verified" }
HS13PREP:HS13	NSD2 PWWP1 Construct not curatedHomo sapiens	Qualitative / associationQuantitative assay verification pendingabstract reported covalent association	No quantitative observation curated	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {} Qualification Primary abstract reports Kd 8.33 µM and C294 covalency; full assay method, construct, uncertainty and exact chemical identity not verified. Main value remains null.	PMID:42442058 · 2026PubMed_primary_article_abstract_only Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": "Kd", "abstract_reported_method": null, "abstract_reported_units": "\u00b5M", "abstract_reported_value": 8.33, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0035", "assay_type": "Quantitative assay verification pending", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0035\", \"assay_type\": \"Quantitative assay verification pending\", \"ccd_id\": null, \"compound_name\": \"HS13\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Abstract\", \"evidence_url\": \"https://doi.org/10.1016/j.bmc.2026.118751\", \"gene\": \"NSD2\", \"interpretation\": \"Abstract describes C294 covalency and an affinity value; full assay details were inaccessible, so numerical pharmacology is intentionally uncurated.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:42442058\", \"role\": \"reported_covalent_ligand\", \"uncertainty\": null, \"units\": null, \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"abstract_only_quantitative_verification_pending\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "HS13", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "abstract_reported_covalent_association", "evidence_location": "Abstract", "evidence_url": "https://doi.org/10.1016/j.bmc.2026.118751", "gene": "NSD2", "interpretation": "Abstract describes C294 covalency and an affinity value; full assay details were inaccessible, so numerical pharmacology is intentionally uncurated.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "not_a_quantitative_affinity_record", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:HS13", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:42442058", "remaining_uncertainty": "Primary abstract reports Kd 8.33 \u00b5M and C294 covalency; full assay method, construct, uncertainty and exact chemical identity not verified. Main value remains null.", "replicates": null, "role": "reported_covalent_ligand", "source_component_url": null, "source_id": "S08", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "PubMed_primary_article_abstract_only", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "abstract_only_quantitative_verification_pending" }
UNC7145PREP:UNC7145Negative / censored	NSD2 PWWP1 208–368Homo sapiens	Qualitative / associationSPR no appreciable bindingnegative binding test	No quantitative observation curated	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO Conditions {"highest_tested_concentration_uM": 20, "phase3_verification": "SV02A", "purified_tags": "AviTag/NSD2(208–368)/polyhistidine; biotinylated", "source_protocol_highest_concentration_uM": 2} Qualification Primary-source concentration discrepancy: Results report no appreciable binding up to 20 uM, but SPR Methods state 2 uM as highest concentration. Neither is converted into a Kd bound; human source review required.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": 0.5, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0007", "assay_type": "SPR no appreciable binding", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0007\", \"assay_type\": \"SPR no appreciable binding\", \"ccd_id\": null, \"compound_name\": \"UNC7145\", \"conditions\": {\"highest_tested_concentration_uM\": 20}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results: Discovery of a potent ligand targeting NSD2-PWWP1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"No appreciable binding up to 20 \u00b5M; this is not a measured infinite Kd.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:34782742\", \"role\": \"negative_control\", \"uncertainty\": null, \"units\": \"\u00b5M\", \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "UNC7145", "conditions": "{\"highest_tested_concentration_uM\": 20, \"phase3_verification\": \"SV02A\", \"purified_tags\": \"AviTag/NSD2(208\u2013368)/polyhistidine; biotinylated\", \"source_protocol_highest_concentration_uM\": 2}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "negative_binding_test", "evidence_location": "Results: Discovery of a potent ligand targeting NSD2-PWWP1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": "No appreciable binding up to 20 \u00b5M; this is not a measured infinite Kd.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "not_a_quantitative_affinity_record", "numeric_value_inferred_from_structure": 0, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:UNC7145", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "Primary-source concentration discrepancy: Results report no appreciable binding up to 20 uM, but SPR Methods state 2 uM as highest concentration. Neither is converted into a Kd bound; human source review required.", "replicates": null, "role": "negative_control", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": null, "source_reported_units": "\u00b5M", "source_reported_value": null, "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": "\u00b5M", "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_full_text_sections_verified" }
UNC6934PREP:UNC6934	NSD2 PWWP1 Construct not curatedHomo sapiens	Unstandardized half-response concentrationNanoBRET PPI half-responsecellular interaction response	= 1.23 ± 0.25 µM	Experimental context Replicates 3 independent replicates Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer DMEM/F12 without phenol red; 4% FBS; 100 U/mL penicillin; 100 ug/mL streptomycin Conditions {"cell_line": "U2OS", "readout": "NSD2 PWWP1–histone H3.3 interaction"} Qualification NanoLuc construct boundaries, readout temperature and SD-versus-SEM are not explicit in the inspected source. Results say EC50 while Discussion says IC50; neutral half-response label retained.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0006", "assay_type": "NanoBRET PPI half-response", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0006\", \"assay_type\": \"NanoBRET PPI half-response\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {\"cell_line\": \"U2OS\", \"readout\": \"NSD2 PWWP1\u2013histone H3.3 interaction\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results: cellular target engagement; Figure 4d\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"Main Results calls this EC50; Discussion calls it IC50. Original value retained without conflating it with Kd.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6XCG\", \"reference_id\": \"PMID:34782742\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 0.25, \"units\": \"\u00b5M\", \"value\": 1.23, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "DMEM/F12 without phenol red; 4% FBS; 100 U/mL penicillin; 100 ug/mL streptomycin", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"cell_line\": \"U2OS\", \"readout\": \"NSD2 PWWP1\u2013histone H3.3 interaction\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": "Results/Figure use EC50, Discussion uses IC50; neutral half-response retained", "evidence_class": "cellular_interaction_response", "evidence_location": "Results: cellular target engagement; Figure 4d", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": "Main Results calls this EC50; Discussion calls it IC50. Original value retained without conflating it with Kd.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "6XCG", "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "NanoLuc construct boundaries, readout temperature and SD-versus-SEM are not explicit in the inspected source. Results say EC50 while Discussion says IC50; neutral half-response label retained.", "replicates": "3 independent replicates", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": "0.25", "source_reported_units": "\u00b5M", "source_reported_value": "1.23", "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": null, "uncertainty": 0.25, "uncertainty_type": null, "uncertainty_uM": 0.25, "unit_conversion": null, "units": "\u00b5M", "value": 1.23, "value_operator": "=", "value_uM": 1.23, "verification_level": "primary_full_text_sections_verified" }
BPPPREP:BPPNegative / censored	NSD2 · PWWP instance unresolved Construct not curatedHomo sapiens	qualitativeBinding selectivity; assay method not verified in Figure S2qualitative binding	Source reports non-detectable affinity in the tested selectivity comparison.	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification No concentration limit, numeric Kd, assay method or construct boundaries assigned without original Supplementary Figure S2. Source says NSD2 PWWP but does not specify PWWP1 versus PWWP2 in the inspected passage; domain_id remains null.	PMID:39031937 · 2024Official publisher full HTML independently inspected: Methods, Results and Figures 1–4 captions; original Supplementary Figure S2 was not retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_PWWP_domain_unspecified_instance", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0032", "assay_type": "Binding selectivity; assay method not verified in Figure S2", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "compound_name": "BPP", "conditions": {}, "domain": null, "domain_id": null, "endpoint": "qualitative", "evidence_class": "qualitative_binding", "evidence_location": "Results: selectivity paragraph referring to Figure S2", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.14981", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": true, "negative_evidence_scope": "Non-detectable binding in the source experimental comparison; assay limits not recovered.", "numeric_transcription_status": "qualitative_or_not_reported", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:BPP", "protein_id": "UP:O96028", "publication_year": 2024, "qualitative_result": "Source reports non-detectable affinity in the tested selectivity comparison.", "reference_id": "PMID:39031937", "remaining_uncertainty": "No concentration limit, numeric Kd, assay method or construct boundaries assigned without original Supplementary Figure S2. Source says NSD2 PWWP but does not specify PWWP1 versus PWWP2 in the inspected passage; domain_id remains null.", "replicates": null, "retrieval_date": "2026-10-26", "role": "experimentally_studied_ligand", "source_id": "P5:S:WEI2024", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Official publisher full HTML independently inspected: Methods, Results and Figures 1\u20134 captions; original Supplementary Figure S2 was not retrieved", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8587PREP:UNC8587	NSD2 · protein-level evidence Construct not curatedHomo sapiens	qualitativeImmunoblot cellular controlqualitative control	No change in H3K36me2 in the matched control condition.	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'KMS11', 'compound_concentration_uM': 20, 'duration_days': 8, 'readout': 'H3K36me2'} Qualification This is a histone-mark cellular observation; it is not an exact NSD2-PWWP1 binding measurement.	PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1 Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0014", "assay_type": "Immunoblot cellular control", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8587", "conditions": { "cell_line": "KMS11", "compound_concentration_uM": 20, "duration_days": 8, "readout": "H3K36me2" }, "domain": null, "domain_id": null, "endpoint": "qualitative", "evidence_class": "qualitative_control", "evidence_location": "Results: UNC8153 degradation leads to loss of H3K36me2; Figure 5B", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "negative_control_scope": "Matched isopropyl analog for the UNC8153 degradation study", "numeric_transcription_status": "qualitative_or_not_reported", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8587", "protein_id": "UP:O96028", "publication_year": 2023, "qualitative_result": "No change in H3K36me2 in the matched control condition.", "reference_id": "PMID:36976643", "remaining_uncertainty": "This is a histone-mark cellular observation; it is not an exact NSD2-PWWP1 binding measurement.", "replicates": null, "retrieval_date": "2026-10-26", "role": "negative_control", "source_id": "P5:S:HANLEY2023", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8884PREP:UNC8884Negative / censored	NSD2 PWWP1 Construct not curatedHomo sapiens	qualitativeSPR qualitative no bindingqualitative control	NB (no binding); no numeric Kd assigned.	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification Table reports NB for binding and ND for degradation endpoints; ND means not determined and is not converted to zero or inactivity.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0024", "assay_type": "SPR qualitative no binding", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8884", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "qualitative", "evidence_class": "qualitative_control", "evidence_location": "Table 1; Supplementary Figure 1", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": true, "negative_control_scope": "Matched NSD2 ligand-binding negative control", "negative_evidence_scope": "Explicit SPR NB result; no numeric threshold or affinity inferred.", "numeric_transcription_status": "qualitative_or_not_reported", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8884", "protein_id": "UP:O96028", "publication_year": 2024, "qualitative_result": "NB (no binding); no numeric Kd assigned.", "reference_id": "PMID:38965384", "remaining_uncertainty": "Table reports NB for binding and ND for degradation endpoints; ND means not determined and is not converted to zero or inactivity.", "replicates": null, "retrieval_date": "2026-10-26", "role": "negative_control", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
MS9715PREP:MS9715	NSD3 · protein-level evidence Construct not curatedHomo sapiens	DC50Immunoblot degradationcellular protein degradation	= 4.9 ± 0.4 µM4.9 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions {'cell_line': 'MOLM-13', 'duration_hours': 48, 'measured_species': 'NSD3S'} Qualification Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.	PMID:34469831 · 2022NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0004", "assay_type": "Immunoblot degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:RWXCSOWWDBANDF-LPFSXYLXSA-N", "compound_name": "MS9715", "conditions": { "cell_line": "MOLM-13", "duration_hours": 48, "measured_species": "NSD3S" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Results; Figure 3E and S1E", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8882712/", "gene": "NSD3", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:MS9715", "protein_id": "UP:Q9BZ95", "publication_year": 2022, "reference_id": "PMID:34469831", "remaining_uncertainty": "Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:XU2022", "source_reported_uncertainty": "0.4", "source_reported_units": "\u00b5M", "source_reported_value": "4.9", "source_verification_scope": "NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected", "uncertainty": 0.4, "uncertainty_type": "SD", "uncertainty_uM": 0.4, "units": "\u00b5M", "value": 4.9, "value_operator": "=", "value_uM": 4.9, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
MS9715PREP:MS9715	NSD3 · protein-level evidence Construct not curatedHomo sapiens	DmaxImmunoblot degradationcellular protein degradation	> 80 percent	Experimental context Replicates 3 independent experiments Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'MOLM-13', 'duration_hours': 48, 'measured_species': 'NSD3S'} Qualification Main text reports greater than 80%; inequality preserved, no exact Dmax assigned.	PMID:34469831 · 2022NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0005", "assay_type": "Immunoblot degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:RWXCSOWWDBANDF-LPFSXYLXSA-N", "compound_name": "MS9715", "conditions": { "cell_line": "MOLM-13", "duration_hours": 48, "measured_species": "NSD3S" }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Results: MS9715 effectively degrades NSD3", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8882712/", "gene": "NSD3", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:MS9715", "protein_id": "UP:Q9BZ95", "publication_year": 2022, "reference_id": "PMID:34469831", "remaining_uncertainty": "Main text reports greater than 80%; inequality preserved, no exact Dmax assigned.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:XU2022", "source_reported_uncertainty": null, "source_reported_units": "percent", "source_reported_value": "80", "source_verification_scope": "NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "percent", "value": 80, "value_operator": "\u003e", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
BI-9321PREP:BI-9321	NSD3 PWWP1 Construct not curatedHomo sapiens	IC50NanoBRET IC50cellular interaction response	= 1.2 ± 0.06 µM1.2 µM · exact unit conversion	Experimental context Replicates 3 independent replicates (Figure 4e; also Supplementary Table 7) Uncertainty definition SD Temperature — Buffer Not curated Conditions {"cell_line": "U2OS"} Qualification The value 1.2 ± 0.06 uM, SD and n=3 were verified in the actual Supplementary Table 7. Full NanoBRET construct and conditions were not inspected. The 1.4 uM direct tracer-engagement endpoint is separate from this histone PPI assay.	PMID:31285596 · 2019Phase 3 actual publisher Supplementary Table 7 and official Figure 3/4 captions; selected indexed accepted-manuscript Methods, full PDF unavailable (403); developer identity page Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0009", "assay_type": "NanoBRET IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0009\", \"assay_type\": \"NanoBRET IC50\", \"ccd_id\": \"EJE\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"BI-9321\", \"conditions\": {\"cell_line\": \"U2OS\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"SGC probe developer report: Overview\", \"evidence_url\": \"https://www.thesgc.org/chemical-probes/bi-9321\", \"gene\": \"NSD3\", \"interpretation\": \"\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6G2O\", \"reference_id\": \"PMID:31285596\", \"role\": \"reported_chemical_probe\", \"uncertainty\": null, \"units\": \"\u00b5M\", \"value\": 1.2, \"value_operator\": \"=\", \"verification_level\": \"primary_developer_assay_report_verified\"}", "buffer": null, "ccd_id": "EJE", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:WOAOENGFAAUUGT-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "BI-9321", "conditions": "{\"cell_line\": \"U2OS\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint_label_status": null, "evidence_class": "cellular_interaction_response", "evidence_location": "SGC probe developer report: Overview", "evidence_url": "https://www.thesgc.org/chemical-probes/bi-9321", "gene": "NSD3", "interpretation": null, "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "6G2O", "preparation_id": "PREP:BI-9321", "protein_id": "UP:Q9BZ95", "qualitative_result": null, "reference_id": "PMID:31285596", "remaining_uncertainty": "The value 1.2 \u00b1 0.06 uM, SD and n=3 were verified in the actual Supplementary Table 7. Full NanoBRET construct and conditions were not inspected. The 1.4 uM direct tracer-engagement endpoint is separate from this histone PPI assay.", "replicates": "3 independent replicates (Figure 4e; also Supplementary Table 7)", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S03", "source_reported_uncertainty": "0.06", "source_reported_units": "\u00b5M", "source_reported_value": "1.2", "source_verification_scope": "Phase 3 actual publisher Supplementary Table 7 and official Figure 3/4 captions; selected indexed accepted-manuscript Methods, full PDF unavailable (403); developer identity page", "temperature_C": null, "uncertainty": 0.06, "uncertainty_type": "SD", "uncertainty_uM": 0.06, "unit_conversion": null, "units": "\u00b5M", "value": 1.2, "value_operator": "=", "value_uM": 1.2, "verification_level": "primary_developer_assay_report_verified" }
BI-9321PREP:BI-9321	NSD3 PWWP1 247–402Homo sapiens	KdITC Kddirect binding affinity	= 1.7 ± 0.04 µM1.7 µM · exact unit conversion	Experimental context Replicates 2 independent measurements Uncertainty definition SD Temperature 15 °C Buffer 50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP Conditions {'buffer': '50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP', 'compound_syringe_concentration_uM': 200, 'construct': 'NSD3 247–402 in pNICBio2, E. coli expressed', 'first_injection_removed': True, 'model': 'single binding site', 'pH': 6.8, 'protein_concentration_uM': 20, 'temperature_C': 15} Qualification Primary manuscript and Methods inspected; individual raw titration files not re-fitted. BI-9321 is a distinct study-specific ITC measurement, not the original 2019 SPR result.	PMID:34469831 · 2022NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected Original observation record { "DMSO_percent": 1, "assay_construct_end": 402, "assay_construct_start": 247, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0001", "assay_type": "ITC Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "buffer": "50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP", "ccd_id": null, "chemical_id": "INCHIKEY:WOAOENGFAAUUGT-UHFFFAOYSA-N", "compound_name": "BI-9321", "conditions": { "buffer": "50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP", "compound_syringe_concentration_uM": 200, "construct": "NSD3 247\u2013402 in pNICBio2, E. coli expressed", "first_injection_removed": true, "model": "single binding site", "pH": 6.8, "protein_concentration_uM": 20, "temperature_C": 15 }, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results and Figure 2B-D; STAR Methods: ITC", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8882712/", "gene": "NSD3", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pH": 6.8, "pdb_id": null, "preparation_id": "PREP:BI-9321", "protein_id": "UP:Q9BZ95", "publication_year": 2022, "reference_id": "PMID:34469831", "remaining_uncertainty": "Primary manuscript and Methods inspected; individual raw titration files not re-fitted. BI-9321 is a distinct study-specific ITC measurement, not the original 2019 SPR result.", "replicates": "2 independent measurements", "retrieval_date": "2026-10-26", "role": "experimentally_studied_ligand", "source_id": "P5:S:XU2022", "source_reported_uncertainty": "0.04", "source_reported_units": "\u00b5M", "source_reported_value": "1.7", "source_verification_scope": "NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected", "temperature_C": 15, "uncertainty": 0.04, "uncertainty_type": "SD", "uncertainty_uM": 0.04, "units": "\u00b5M", "value": 1.7, "value_operator": "=", "value_uM": 1.7, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
BI-9321PREP:BI-9321	NSD3 PWWP1 263–398Homo sapiens	KdSPR Kddirect binding affinity	= 166 ± 3 nM0.166 µM · exact unit conversion	Experimental context Replicates 3 independent experiments (Figure 3a); 4 (Supplementary Table 7); unresolved source discrepancy Uncertainty definition SD Temperature — Buffer 50 mM Tris; 150 mM NaCl; 1 mM TCEP; 0.005% Tween-20; 2% DMSO Conditions {} Qualification Primary replicate conflict: Figure 3a states n=3 independent experiments, while Supplementary Table 7 states SPR n=4; both report SD. Indexed primary SPR Methods supply residues 263–398 and buffer, but the full PDF was unavailable. Exact assay temperature, mutations and per-dose contact times remain not inspected.	PMID:31285596 · 2019Phase 3 actual publisher Supplementary Table 7 and official Figure 3/4 captions; selected indexed accepted-manuscript Methods, full PDF unavailable (403); developer identity page Original observation record { "DMSO_percent": 2.0, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 398, "assay_construct_start": 263, "assay_record_id": "PWWP:ASSAY:0008", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0008\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"EJE\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"BI-9321\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"SGC probe developer report: Overview\", \"evidence_url\": \"https://www.thesgc.org/chemical-probes/bi-9321\", \"gene\": \"NSD3\", \"interpretation\": \"Primary developer website; original full article assay table was not retrieved.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6G2O\", \"reference_id\": \"PMID:31285596\", \"role\": \"reported_chemical_probe\", \"uncertainty\": null, \"units\": \"nM\", \"value\": 166, \"value_operator\": \"=\", \"verification_level\": \"primary_developer_assay_report_verified\"}", "buffer": "50 mM Tris; 150 mM NaCl; 1 mM TCEP; 0.005% Tween-20; 2% DMSO", "ccd_id": "EJE", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:WOAOENGFAAUUGT-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "BI-9321", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "SGC probe developer report: Overview", "evidence_url": "https://www.thesgc.org/chemical-probes/bi-9321", "gene": "NSD3", "interpretation": "Primary developer website; original full article assay table was not retrieved.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 8.0, "pdb_id": "6G2O", "preparation_id": "PREP:BI-9321", "protein_id": "UP:Q9BZ95", "qualitative_result": null, "reference_id": "PMID:31285596", "remaining_uncertainty": "Primary replicate conflict: Figure 3a states n=3 independent experiments, while Supplementary Table 7 states SPR n=4; both report SD. Indexed primary SPR Methods supply residues 263\u2013398 and buffer, but the full PDF was unavailable. Exact assay temperature, mutations and per-dose contact times remain not inspected.", "replicates": "3 independent experiments (Figure 3a); 4 (Supplementary Table 7); unresolved source discrepancy", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S03", "source_reported_uncertainty": "3.0", "source_reported_units": "nM", "source_reported_value": "166", "source_verification_scope": "Phase 3 actual publisher Supplementary Table 7 and official Figure 3/4 captions; selected indexed accepted-manuscript Methods, full PDF unavailable (403); developer identity page", "temperature_C": null, "uncertainty": 3.0, "uncertainty_type": "SD", "uncertainty_uM": 0.003, "unit_conversion": null, "units": "nM", "value": 166.0, "value_operator": "=", "value_uM": 0.166, "verification_level": "primary_developer_assay_report_verified" }
MS9715PREP:MS9715	NSD3 PWWP1 247–402Homo sapiens	KdITC Kddirect binding affinity	= 1.3 ± 0.17 µM1.3 µM · exact unit conversion	Experimental context Replicates 2 independent measurements Uncertainty definition SD Temperature 15 °C Buffer 50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP Conditions {'buffer': '50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP', 'compound_syringe_concentration_uM': 200, 'construct': 'NSD3 247–402 in pNICBio2, E. coli expressed', 'first_injection_removed': True, 'model': 'single binding site', 'pH': 6.8, 'protein_concentration_uM': 20, 'temperature_C': 15} Qualification Primary manuscript and Methods inspected; individual raw titration files not re-fitted. BI-9321 is a distinct study-specific ITC measurement, not the original 2019 SPR result.	PMID:34469831 · 2022NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected Original observation record { "DMSO_percent": 1, "assay_construct_end": 402, "assay_construct_start": 247, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0002", "assay_type": "ITC Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "buffer": "50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP", "ccd_id": null, "chemical_id": "INCHIKEY:RWXCSOWWDBANDF-LPFSXYLXSA-N", "compound_name": "MS9715", "conditions": { "buffer": "50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP", "compound_syringe_concentration_uM": 200, "construct": "NSD3 247\u2013402 in pNICBio2, E. coli expressed", "first_injection_removed": true, "model": "single binding site", "pH": 6.8, "protein_concentration_uM": 20, "temperature_C": 15 }, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results and Figure 2B-D; STAR Methods: ITC", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8882712/", "gene": "NSD3", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pH": 6.8, "pdb_id": null, "preparation_id": "PREP:MS9715", "protein_id": "UP:Q9BZ95", "publication_year": 2022, "reference_id": "PMID:34469831", "remaining_uncertainty": "Primary manuscript and Methods inspected; individual raw titration files not re-fitted. BI-9321 is a distinct study-specific ITC measurement, not the original 2019 SPR result.", "replicates": "2 independent measurements", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:XU2022", "source_reported_uncertainty": "0.17", "source_reported_units": "\u00b5M", "source_reported_value": "1.3", "source_verification_scope": "NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected", "temperature_C": 15, "uncertainty": 0.17, "uncertainty_type": "SD", "uncertainty_uM": 0.17, "units": "\u00b5M", "value": 1.3, "value_operator": "=", "value_uM": 1.3, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
MS9715NPREP:MS9715N	NSD3 PWWP1 247–402Homo sapiens	KdITC Kddirect binding affinity	= 1.62 ± 0.33 µM1.62 µM · exact unit conversion	Experimental context Replicates 2 independent measurements Uncertainty definition SD Temperature 15 °C Buffer 50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP Conditions {'buffer': '50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP', 'compound_syringe_concentration_uM': 200, 'construct': 'NSD3 247–402 in pNICBio2, E. coli expressed', 'first_injection_removed': True, 'model': 'single binding site', 'pH': 6.8, 'protein_concentration_uM': 20, 'temperature_C': 15} Qualification Primary manuscript and Methods inspected; individual raw titration files not re-fitted. BI-9321 is a distinct study-specific ITC measurement, not the original 2019 SPR result.	PMID:34469831 · 2022NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected Original observation record { "DMSO_percent": 1, "assay_construct_end": 402, "assay_construct_start": 247, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0003", "assay_type": "ITC Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "buffer": "50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP", "ccd_id": null, "chemical_id": null, "compound_name": "MS9715N", "conditions": { "buffer": "50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP", "compound_syringe_concentration_uM": 200, "construct": "NSD3 247\u2013402 in pNICBio2, E. coli expressed", "first_injection_removed": true, "model": "single binding site", "pH": 6.8, "protein_concentration_uM": 20, "temperature_C": 15 }, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results and Figure 2B-D; STAR Methods: ITC", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8882712/", "gene": "NSD3", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pH": 6.8, "pdb_id": null, "preparation_id": "PREP:MS9715N", "protein_id": "UP:Q9BZ95", "publication_year": 2022, "reference_id": "PMID:34469831", "remaining_uncertainty": "Primary manuscript and Methods inspected; individual raw titration files not re-fitted. BI-9321 is a distinct study-specific ITC measurement, not the original 2019 SPR result.", "replicates": "2 independent measurements", "retrieval_date": "2026-10-26", "role": "negative_control", "source_id": "P5:S:XU2022", "source_reported_uncertainty": "0.33", "source_reported_units": "\u00b5M", "source_reported_value": "1.62", "source_verification_scope": "NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected", "temperature_C": 15, "uncertainty": 0.33, "uncertainty_type": "SD", "uncertainty_uM": 0.33, "units": "\u00b5M", "value": 1.62, "value_operator": "=", "value_uM": 1.62, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC6934PREP:UNC6934Negative / censored	NSD3 PWWP1 263–398Homo sapiens	KdSPR Kd counter-screencensored binding limit	> 30 µM	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition Not curated; do not assume SD or SEM Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD3 263–398", "purified_tags": "N-terminal 6His-TEV retained"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 398, "assay_construct_start": 263, "assay_record_id": "PWWP11:ASSAY:0001", "assay_type": "SPR Kd counter-screen", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "V01", "ccd_mapping_source": null, "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD3 263\u2013398\", \"purified_tags\": \"N-terminal 6His-TEV retained\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint_label_status": null, "evidence_class": "censored_binding_limit", "evidence_location": "Table 1 NSD3 (263\u2013398) column", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD3", "interpretation": "Single counter-target experiment; not evidence of proteome-wide selectivity. No-binding rows have no inferred numerical threshold.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:UNC6934", "protein_id": "UP:Q9BZ95", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "counter_target_assay", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": "\u00b5M", "value": 30.0, "value_operator": "\u003e", "value_uM": 30.0, "verification_level": null }
compound 11 (Collie 2025)PREP:COLLIE2025_11Negative / censored	NSD3 PWWP1 263–398Homo sapiens	KdSPR Kd counter-screennegative binding test	No binding	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition Not curated; do not assume SD or SEM Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD3 263–398", "purified_tags": "N-terminal 6His-TEV retained"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 398, "assay_construct_start": 263, "assay_record_id": "PWWP11:ASSAY:0005", "assay_type": "SPR Kd counter-screen", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 11 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD3 263\u2013398\", \"purified_tags\": \"N-terminal 6His-TEV retained\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint_label_status": null, "evidence_class": "negative_binding_test", "evidence_location": "Table 1 NSD3 (263\u2013398) column", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD3", "interpretation": "Single counter-target experiment; not evidence of proteome-wide selectivity. No-binding rows have no inferred numerical threshold.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_11", "protein_id": "UP:Q9BZ95", "qualitative_result": "No binding", "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "counter_target_assay", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": null }
compound 12 (Collie 2025)PREP:COLLIE2025_12Negative / censored	NSD3 PWWP1 263–398Homo sapiens	KdSPR Kd counter-screennegative binding test	No binding	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition Not curated; do not assume SD or SEM Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD3 263–398", "purified_tags": "N-terminal 6His-TEV retained"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 398, "assay_construct_start": 263, "assay_record_id": "PWWP11:ASSAY:0006", "assay_type": "SPR Kd counter-screen", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 12 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD3 263\u2013398\", \"purified_tags\": \"N-terminal 6His-TEV retained\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint_label_status": null, "evidence_class": "negative_binding_test", "evidence_location": "Table 1 NSD3 (263\u2013398) column", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD3", "interpretation": "Single counter-target experiment; not evidence of proteome-wide selectivity. No-binding rows have no inferred numerical threshold.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_12", "protein_id": "UP:Q9BZ95", "qualitative_result": "No binding", "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "counter_target_assay", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": null }
compound 13 (Collie 2025)PREP:COLLIE2025_13Negative / censored	NSD3 PWWP1 263–398Homo sapiens	KdSPR Kd counter-screennegative binding test	No binding	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition Not curated; do not assume SD or SEM Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD3 263–398", "purified_tags": "N-terminal 6His-TEV retained"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 398, "assay_construct_start": 263, "assay_record_id": "PWWP11:ASSAY:0007", "assay_type": "SPR Kd counter-screen", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 13 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD3 263\u2013398\", \"purified_tags\": \"N-terminal 6His-TEV retained\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint_label_status": null, "evidence_class": "negative_binding_test", "evidence_location": "Table 1 NSD3 (263\u2013398) column", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD3", "interpretation": "Single counter-target experiment; not evidence of proteome-wide selectivity. No-binding rows have no inferred numerical threshold.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_13", "protein_id": "UP:Q9BZ95", "qualitative_result": "No binding", "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "counter_target_assay", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": null }
compound 5 (Collie 2025)PREP:COLLIE2025_5Negative / censored	NSD3 PWWP1 263–398Homo sapiens	KdSPR Kd counter-screencensored binding limit	> 30 µM	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition Not curated; do not assume SD or SEM Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD3 263–398", "purified_tags": "N-terminal 6His-TEV retained"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 398, "assay_construct_start": 263, "assay_record_id": "PWWP11:ASSAY:0002", "assay_type": "SPR Kd counter-screen", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 5 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD3 263\u2013398\", \"purified_tags\": \"N-terminal 6His-TEV retained\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint_label_status": null, "evidence_class": "censored_binding_limit", "evidence_location": "Table 1 NSD3 (263\u2013398) column", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD3", "interpretation": "Single counter-target experiment; not evidence of proteome-wide selectivity. No-binding rows have no inferred numerical threshold.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_5", "protein_id": "UP:Q9BZ95", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "counter_target_assay", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": "\u00b5M", "value": 30.0, "value_operator": "\u003e", "value_uM": 30.0, "verification_level": null }
compound 6 (Collie 2025)PREP:COLLIE2025_6Negative / censored	NSD3 PWWP1 263–398Homo sapiens	KdSPR Kd counter-screencensored binding limit	> 30 µM	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition Not curated; do not assume SD or SEM Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD3 263–398", "purified_tags": "N-terminal 6His-TEV retained"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 398, "assay_construct_start": 263, "assay_record_id": "PWWP11:ASSAY:0003", "assay_type": "SPR Kd counter-screen", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 6 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD3 263\u2013398\", \"purified_tags\": \"N-terminal 6His-TEV retained\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint_label_status": null, "evidence_class": "censored_binding_limit", "evidence_location": "Table 1 NSD3 (263\u2013398) column", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD3", "interpretation": "Single counter-target experiment; not evidence of proteome-wide selectivity. No-binding rows have no inferred numerical threshold.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_6", "protein_id": "UP:Q9BZ95", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "counter_target_assay", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": "\u00b5M", "value": 30.0, "value_operator": "\u003e", "value_uM": 30.0, "verification_level": null }
compound 7 (Collie 2025)PREP:COLLIE2025_7Negative / censored	NSD3 PWWP1 263–398Homo sapiens	KdSPR Kd counter-screencensored binding limit	> 30 µM	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition Not curated; do not assume SD or SEM Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD3 263–398", "purified_tags": "N-terminal 6His-TEV retained"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 398, "assay_construct_start": 263, "assay_record_id": "PWWP11:ASSAY:0004", "assay_type": "SPR Kd counter-screen", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "A1IEF", "ccd_mapping_source": null, "chemical_id": "INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "compound 7 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD3 263\u2013398\", \"purified_tags\": \"N-terminal 6His-TEV retained\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint_label_status": null, "evidence_class": "censored_binding_limit", "evidence_location": "Table 1 NSD3 (263\u2013398) column", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD3", "interpretation": "Single counter-target experiment; not evidence of proteome-wide selectivity. No-binding rows have no inferred numerical threshold.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_7", "protein_id": "UP:Q9BZ95", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "counter_target_assay", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": "\u00b5M", "value": 30.0, "value_operator": "\u003e", "value_uM": 30.0, "verification_level": null }
BI-9466PREP:BI-9466	NSD3 PWWP1 Construct not curatedHomo sapiens	qualitativeOfficial SGC control activity summaryqualitative control	SGC reports approximately 200-fold lower activity than BI-9321 and designates it the matched negative control.	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification Endpoint-specific absolute numeric value and test construct not transcribed; no numerical affinity derived from the fold statement.	P5:S:SGC_BI9321Official SGC probe page; explicit BI-9466 SMILES, InChI and InChIKey corroborated against PubChem Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0008", "assay_type": "Official SGC control activity summary", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:SFZHMKDAVPIXRB-UHFFFAOYSA-N", "compound_name": "BI-9466", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:Q9BZ95:PWWP1", "endpoint": "qualitative", "evidence_class": "qualitative_control", "evidence_location": "Overview", "evidence_url": "https://www.thesgc.org/chemical-probes/bi-9321", "gene": "NSD3", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "negative_control_scope": "Weaker NSD3-PWWP1 antagonist; use matched dose response per source", "numeric_transcription_status": "qualitative_or_not_reported", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:BI-9466", "protein_id": "UP:Q9BZ95", "publication_year": null, "qualitative_result": "SGC reports approximately 200-fold lower activity than BI-9321 and designates it the matched negative control.", "reference_id": "P5:S:SGC_BI9321", "remaining_uncertainty": "Endpoint-specific absolute numeric value and test construct not transcribed; no numerical affinity derived from the fold statement.", "replicates": null, "retrieval_date": "2026-10-26", "role": "negative_control", "source_id": "P5:S:SGC_BI9321", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Official SGC probe page; explicit BI-9466 SMILES, InChI and InChIKey corroborated against PubChem", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "official_probe_resource_verified", "version": "phase5.chemical_curation.1.1.0" }
MS9715NPREP:MS9715N	NSD3 · protein-level evidence Construct not curatedHomo sapiens	qualitativeImmunoblot degradation controlqualitative control	No NSD3 depletion observed in the matched control comparison.	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'cell_lines': ['EOL-1', 'MM1.S'], 'duration_hours': 48} Qualification Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.	PMID:34469831 · 2022NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0006", "assay_type": "Immunoblot degradation control", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "MS9715N", "conditions": { "cell_lines": [ "EOL-1", "MM1.S" ], "duration_hours": 48 }, "domain": null, "domain_id": null, "endpoint": "qualitative", "evidence_class": "qualitative_control", "evidence_location": "Results; Figure 3A-B; Figure S1C-D", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8882712/", "gene": "NSD3", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "negative_control_scope": "Productive VHL recruitment and degradation; retains direct NSD3-PWWP1 binding", "numeric_transcription_status": "qualitative_or_not_reported", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:MS9715N", "protein_id": "UP:Q9BZ95", "publication_year": 2022, "qualitative_result": "No NSD3 depletion observed in the matched control comparison.", "reference_id": "PMID:34469831", "remaining_uncertainty": "Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.", "replicates": null, "retrieval_date": "2026-10-26", "role": "negative_control", "source_id": "P5:S:XU2022", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
NbH10PREP:NbH10	PSIP1 · protein-level evidence Construct not curatedVariant: wild type as reportedHomo sapiens	IC50AlphaScreen inhibition IC50full protein chromatin interaction inhibition	= 0.94 ± 0.15 µM0.94 µM · exact unit conversion	Reviewed target attribution: Primary Results explicitly identify full-length LEDGF/p75; Methods specify FLAG-LEDGF/p75 and H3K36me3 nucleosomes. Domain-specific evidence counts must use this reviewed context while preserving the frozen source record. Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {'binding_partner': 'H3K36me3-modified nucleosomes', 'buffer': '25 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM MgCl2; 1 mM DTT; 0.1% Tween 20; 0.1% BSA', 'nucleosome_concentration_nM': 5, 'preincubation': '30 minutes at 4 °C; after nucleosome addition 1 hour at 4 °C; after beads 1 hour at room temperature', 'target': 'full-length FLAG-LEDGF/p75', 'target_concentration_nM': 10} Qualification Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.	PMID:42194065 · 2026local_primary_full_text_XML; Table 1, methods and Results inspected Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0032", "assay_type": "AlphaScreen inhibition IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0032\", \"assay_type\": \"AlphaScreen inhibition IC50\", \"ccd_id\": null, \"compound_name\": \"NbH10\", \"conditions\": {\"partner\": \"H3K36me3 nucleosomes\", \"protein\": \"full-length LEDGF/p75\"}, \"domain\": \"PWWP\", \"evidence_location\": \"Section 3.6; Figure 3\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/\", \"gene\": \"PSIP1\", \"interpretation\": \"Macromolecular binder. NbH10-alone crystal coordinates do not establish a PWWP-bound complex.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:42194065\", \"role\": \"experimentally_studied_macromolecular_binder\", \"uncertainty\": 0.15, \"units\": \"\u00b5M\", \"value\": 0.94, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "named_macromolecule_no_full_sequence_identity", "component_context": null, "compound_name": "NbH10", "conditions": "{\"partner\": \"H3K36me3 nucleosomes\", \"protein\": \"full-length LEDGF/p75\"}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:O75475:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Section 3.6; Figure 3", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/", "gene": "PSIP1", "interpretation": "Macromolecular binder. NbH10-alone crystal coordinates do not establish a PWWP-bound complex.", "molecular_entity_type": "nanobody", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:NbH10", "protein_id": "UP:O75475", "qualitative_result": null, "reference_id": "PMID:42194065", "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.", "replicates": null, "role": "experimentally_studied_macromolecular_binder", "source_component_url": null, "source_id": "S06", "source_reported_uncertainty": "0.15", "source_reported_units": "\u00b5M", "source_reported_value": "0.94", "source_verification_scope": "local_primary_full_text_XML; Table 1, methods and Results inspected", "temperature_C": null, "uncertainty": 0.15, "uncertainty_type": null, "uncertainty_uM": 0.15, "unit_conversion": null, "units": "\u00b5M", "value": 0.94, "value_operator": "=", "value_uM": 0.94, "verification_level": "primary_full_text_sections_verified" }
BPPPREP:BPP	PSIP1 PWWP Construct not curatedHomo sapiens	KdITC Kddirect binding affinity	= 27 ± 2 µM27 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting_error Temperature — Buffer Not curated Conditions {} Qualification Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.	PMID:39031937 · 2024primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0012", "assay_type": "ITC Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0012\", \"assay_type\": \"ITC Kd\", \"ccd_id\": \"A1D73\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"BPP\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results; Figure 4B\", \"evidence_url\": \"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981\", \"gene\": \"PSIP1\", \"interpretation\": \"Binding is established in solution; no PSIP1\u2013BPP crystal structure is assigned here.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:39031937\", \"role\": \"experimentally_studied_fragment_ligand\", \"uncertainty\": 2, \"units\": \"\u00b5M\", \"value\": 27, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "A1D73", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "BPP", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:O75475:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 4B", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981", "gene": "PSIP1", "interpretation": "Binding is established in solution; no PSIP1\u2013BPP crystal structure is assigned here.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:BPP", "protein_id": "UP:O75475", "qualitative_result": null, "reference_id": "PMID:39031937", "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.", "replicates": null, "role": "experimentally_studied_fragment_ligand", "source_component_url": null, "source_id": "S04", "source_reported_uncertainty": "2", "source_reported_units": "\u00b5M", "source_reported_value": "27", "source_verification_scope": "primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved", "temperature_C": null, "uncertainty": 2.0, "uncertainty_type": "fitting_error", "uncertainty_uM": 2.0, "unit_conversion": null, "units": "\u00b5M", "value": 27.0, "value_operator": "=", "value_uM": 27.0, "verification_level": "primary_full_text_sections_verified" }
NbH10PREP:NbH10	PSIP1 PWWP1 1–90Homo sapiens	KdSPR apparent Kddirect binding affinity	= 94.4 ± 9.1 nM0.0944 µM · exact unit conversion	Experimental context Replicates 3 Uncertainty definition SD Temperature 25 °C Buffer 50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20 Conditions {"protein_construct": "PSIP1 1–90", "replicates": 3, "uncertainty_type": "SD"} Qualification Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody–PWWP complex.	PMID:42194065 · 2026local_primary_full_text_XML_table_and_methods_verified Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 90, "assay_construct_start": 1, "assay_record_id": "PWWP11:ASSAY:0008", "assay_type": "SPR apparent Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "named_macromolecule_no_full_sequence_identity", "component_context": null, "compound_name": "NbH10", "conditions": "{\"protein_construct\": \"PSIP1 1\u201390\", \"replicates\": 3, \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O75475:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1 NbH10 column; SPR methods", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/", "gene": "PSIP1", "interpretation": "Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody\u2013PWWP complex.", "molecular_entity_type": "nanobody", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:NbH10", "protein_id": "UP:O75475", "qualitative_result": null, "reference_id": "PMID:42194065", "remaining_uncertainty": null, "replicates": "3", "role": "macromolecular_binder", "source_component_url": null, "source_id": "S06", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified", "temperature_C": 25, "uncertainty": 9.1, "uncertainty_type": "SD", "uncertainty_uM": 0.0091, "unit_conversion": null, "units": "nM", "value": 94.4, "value_operator": "=", "value_uM": 0.0944, "verification_level": null }

PWWP Compounds& Bioactivity

13 matching new observations · 12 preparations

132 matching profiles

PWWP Compounds
& Bioactivity