HS13

Identity / evidence review pendingPreparation identity unresolved

Complete deposited identity retained; no connectivity-only or name-only merge.

1 assay / association records1 preparations0 research-ligand PDB entries

No deposited PWWP research-ligand geometry is classified for this preparation in the selected release. Deposition of a buffer/ion is not binding evidence.

Clinical / development status: Not independently curated. A chemical probe classification does not imply medicinal approval.

Identity is separate from preparation

Chemical identity

Measured target and original context

Bioactivity & experimental observations

Download assay CSV

Each row is one source-specific observation. Repeated measurements of this compound are not independent compounds. Direct binding, functional displacement and cellular outcomes retain their distinct endpoints.

Preparation	Tested target & construct	Method / endpoint	Original observation	Conditions & uncertainty	Source & verification
HS13PREP:HS13	NSD2 PWWP1 Construct not curatedHomo sapiens	Qualitative / associationQuantitative assay verification pendingabstract reported covalent association	No quantitative observation curated	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {} Qualification Primary abstract reports Kd 8.33 µM and C294 covalency; full assay method, construct, uncertainty and exact chemical identity not verified. Main value remains null.	PMID:42442058 · 2026PubMed_primary_article_abstract_only Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": "Kd", "abstract_reported_method": null, "abstract_reported_units": "\u00b5M", "abstract_reported_value": 8.33, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0035", "assay_type": "Quantitative assay verification pending", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0035\", \"assay_type\": \"Quantitative assay verification pending\", \"ccd_id\": null, \"compound_name\": \"HS13\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Abstract\", \"evidence_url\": \"https://doi.org/10.1016/j.bmc.2026.118751\", \"gene\": \"NSD2\", \"interpretation\": \"Abstract describes C294 covalency and an affinity value; full assay details were inaccessible, so numerical pharmacology is intentionally uncurated.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:42442058\", \"role\": \"reported_covalent_ligand\", \"uncertainty\": null, \"units\": null, \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"abstract_only_quantitative_verification_pending\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "HS13", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "abstract_reported_covalent_association", "evidence_location": "Abstract", "evidence_url": "https://doi.org/10.1016/j.bmc.2026.118751", "gene": "NSD2", "interpretation": "Abstract describes C294 covalency and an affinity value; full assay details were inaccessible, so numerical pharmacology is intentionally uncurated.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "not_a_quantitative_affinity_record", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:HS13", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:42442058", "remaining_uncertainty": "Primary abstract reports Kd 8.33 \u00b5M and C294 covalency; full assay method, construct, uncertainty and exact chemical identity not verified. Main value remains null.", "replicates": null, "role": "reported_covalent_ligand", "source_component_url": null, "source_id": "S08", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "PubMed_primary_article_abstract_only", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "abstract_only_quantitative_verification_pending" }

Preparation

Tested target & construct

Method / endpoint

Original observation

Conditions & uncertainty

Source & verification

HS13PREP:HS13

NSD2 PWWP1
Construct not curatedHomo sapiens

Qualitative / associationQuantitative assay verification pendingabstract reported covalent association

No quantitative observation curated

Experimental context

Replicates: Not curated
Uncertainty definition: Not curated; do not assume SD or SEM
Temperature: —
Buffer: Not curated
Conditions: {}
Qualification: Primary abstract reports Kd 8.33 µM and C294 covalency; full assay method, construct, uncertainty and exact chemical identity not verified. Main value remains null.

PMID:42442058 · 2026PubMed_primary_article_abstract_only

Original observation record

{
  "DMSO_percent": null,
  "abstract_reported_endpoint": "Kd",
  "abstract_reported_method": null,
  "abstract_reported_units": "\u00b5M",
  "abstract_reported_value": 8.33,
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_record_id": "PWWP:ASSAY:0035",
  "assay_type": "Quantitative assay verification pending",
  "assay_value_transferred_to_modeled_stereoisomers": 0,
  "audit_decision": "retain_with_explicit_endpoint_and_identity",
  "baseline_assay_record": 1,
  "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0035\", \"assay_type\": \"Quantitative assay verification pending\", \"ccd_id\": null, \"compound_name\": \"HS13\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Abstract\", \"evidence_url\": \"https://doi.org/10.1016/j.bmc.2026.118751\", \"gene\": \"NSD2\", \"interpretation\": \"Abstract describes C294 covalency and an affinity value; full assay details were inaccessible, so numerical pharmacology is intentionally uncurated.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:42442058\", \"role\": \"reported_covalent_ligand\", \"uncertainty\": null, \"units\": null, \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"abstract_only_quantitative_verification_pending\"}",
  "buffer": null,
  "ccd_id": null,
  "ccd_mapping_source": null,
  "chemical_id": null,
  "chemical_identity_status": "paper_label_exact_identity_unresolved",
  "component_context": null,
  "compound_name": "HS13",
  "conditions": "{}",
  "crystallographic_component_ids": null,
  "domain": "PWWP1",
  "domain_id": "UP:O96028:PWWP1",
  "endpoint_label_status": null,
  "evidence_class": "abstract_reported_covalent_association",
  "evidence_location": "Abstract",
  "evidence_url": "https://doi.org/10.1016/j.bmc.2026.118751",
  "gene": "NSD2",
  "interpretation": "Abstract describes C294 covalency and an affinity value; full assay details were inaccessible, so numerical pharmacology is intentionally uncurated.",
  "molecular_entity_type": "small_molecule_preparation",
  "numeric_transcription_status": "not_a_quantitative_affinity_record",
  "numeric_value_inferred_from_structure": 0,
  "pH": null,
  "pdb_id": null,
  "preparation_id": "PREP:HS13",
  "protein_id": "UP:O96028",
  "qualitative_result": null,
  "reference_id": "PMID:42442058",
  "remaining_uncertainty": "Primary abstract reports Kd 8.33 \u00b5M and C294 covalency; full assay method, construct, uncertainty and exact chemical identity not verified. Main value remains null.",
  "replicates": null,
  "role": "reported_covalent_ligand",
  "source_component_url": null,
  "source_id": "S08",
  "source_reported_uncertainty": null,
  "source_reported_units": null,
  "source_reported_value": null,
  "source_verification_scope": "PubMed_primary_article_abstract_only",
  "temperature_C": null,
  "uncertainty": null,
  "uncertainty_type": null,
  "uncertainty_uM": null,
  "unit_conversion": null,
  "units": null,
  "value": null,
  "value_operator": null,
  "value_uM": null,
  "verification_level": "abstract_only_quantitative_verification_pending"
}

Exact deposited instances

Experimental structures

No verified exact-identity bound structure

The biochemical or cellular evidence remains fully available. No artificial pose has been assigned.

Preparations, identities and controls remain separate

Experimental context

HS13

PREP:HS13

Complete deposited identity retained; no connectivity-only or name-only merge.

assay preparation

Complete preparation record

{
  "chemical_id": null,
  "entity_type": "small_molecule_preparation",
  "exact_ccd_id": null,
  "identity_note": "Complete deposited identity retained; no connectivity-only or name-only merge.",
  "identity_status": "paper_label_exact_identity_unresolved",
  "modeled_candidate_ccd_ids": "[]",
  "modeled_candidate_chemical_ids": "[]",
  "name": "HS13",
  "preparation_id": "PREP:HS13",
  "role": "assay_preparation",
  "sequence_if_defined": null
}

Provenance and reproducibility

Sources & downloads

https://doi.org/10.1016/j.bmc.2026.118751

Download complete evidence JSON Curation methods & source receipts

Snapshot 2026-10-26 · phase5-1.0.0 · original measurements, operators, constructs and uncertainty are retained in the exports.