Versioned source-reviewed overlay · Phase 6B

Experimental Bioactivity

Primary-source observations, original endpoints, explicit target/construct scope and documented gaps. Phase 5 and Phase 6A remain frozen.

All compound classifications

13additional observed outcomes

12new source preparations

2NSD2 PWWP1 competition observations

150independently triaged source candidates (not validated binders)

Compound / identifierProteinExact domain

Assay, construct, verification, source and structural status

EndpointMethodEvidence type Observed outcomeTarget scope Structure relationship Experimental constructVariantPrimary source / DOI Exact concentration minimum (µM)Exact concentration maximum (µM)Source verification status

All filter predicates must match one observation. Only an exact quantitative endpoint may be filtered by concentration. Units are normalized for filtering without altering the source result.

13 matching observations · 12 preparations

Phase 6B additions only; Phase 6A's baseline 80 remain separately accessible.

Preparation	Target and scope	Method and original endpoint	Source and verification	Structure / limitations
UNC10088published name only exact assayed identity unresolved	NSD2 PWWP1 — exact domainHis-tagged NSD2 PWWP1; exact sequence boundaries not independently extracted	TR-FRET competitive displacement of biotinylated UNC7096 IC50 =248 ± 22 nMSEM · n=3 independent experiments	10.1038/s41467-026-72235-9Figure 2h and Supplementary Figure 3Daccepted with bounded interpretation	Transformed compound in ternary complex — not unmodified parent binary Interpretation and limitations positive competitive engagement; not an equilibrium Kd The prepared chemical parent is not stereochemistry/batch resolved; IC50 not Kd. Do not count FBXO22-conditional results as independent chemical identities. Without FBXO22 FIST domain
UNC10088published name only exact assayed identity unresolved	NSD2 PWWP1 — exact domainHis-tagged NSD2 PWWP1 + FBXO22 FIST; exact sequence boundaries not independently extracted	TR-FRET competitive displacement of biotinylated UNC7096 IC50 =101 ± 8 nMSEM · n=3 independent experiments	10.1038/s41467-026-72235-9Figure 2h, Supplementary Figure 3D and Resultsaccepted with bounded interpretation	Transformed compound in ternary complex — not unmodified parent binary Interpretation and limitations positive cooperative engagement in a ternary context; not an equilibrium Kd Two different experimental contexts for one preparation; do not average or replace one with another. With wild-type FBXO22 FIST domain; source-reported cooperative factor alpha = 3.3, not 248/101
UNC10415667published name only exact assayed identity unresolved	NSD2 — cellular, whole proteinU2OS NSD2-HiBiT cells	Cellular HiBiT protein-degradation dose response DC50 =460 nMfigure caption reports SEM but numeric uncertainty was not independently extracted · n=2 independent experiments (final 2026 article)	10.1038/s41467-026-72235-9Figure 3e legend and Resultsaccepted with bounded interpretation	Transformed compound in ternary complex — not unmodified parent binary Interpretation and limitations cellular whole-protein depletion; no isolated-domain affinity Do not copy this whole-protein DC50 to NSD2 PWWP1. 75% at 1 µM for 6 hours is a separate endpoint, not the same dose-response value. U2OS NSD2 HiBiT dose response
UNC12149published name only exact assayed identity unresolved	NSD2 — cellular, whole proteinU2OS NSD2-HiBiT cells	Cellular HiBiT protein-degradation dose response DC50 =120 nMfigure caption reports SEM but numeric uncertainty was not independently extracted · n=2 independent experiments (final 2026 article)	10.1038/s41467-026-72235-9Figure 4h legend and Resultsaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations cellular whole-protein depletion; no isolated-domain affinity Do not transfer cellular DC50 to a particular PWWP-domain biochemical binding constant. U2OS NSD2 HiBiT dose response
SYC2parent graph verified assayed material unresolved	NSD3 — PWWP instance unresolvedConstruct not verified	SPR (publisher compound table header) Kd =0.07 ± 0.01 µMpublished ± statistic not independently confirmed · Replicate information not extracted	10.1021/acs.jmedchem.6c00983Publisher compound table row 28; SPR Kd (µM) column, corroborated by abstractaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations reported PWWP affinity, but PWWP1 vs PWWP2 attribution remains unverified Published SMILES and SPR column verified; original construct, statistic definition, replicate count and tested lot/salt remain unresolved. Not assignable to NSD3 PWWP1/PWWP2. Specific experimental conditions not fully extracted
SYB7parent graph verified assayed material unresolved	NSD3 — PWWP instance unresolvedConstruct not verified	SPR (publisher compound table header) Kd >20 µMcensored lower bound, no fitted uncertainty · Replicate information not extracted	10.1021/acs.jmedchem.6c00983Publisher source compound table row 20; SPR Kd (µM) columnaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations Source reports Kd >20 µM; do NOT interpret as exact Kd, positive binder or categorical inactivity Censored lower bound only; no numeric exact Kd or biological inactivity conclusion. Original article table lacks complete assay condition and tested construct details
SYB10parent graph verified assayed material unresolved	NSD3 — PWWP instance unresolvedConstruct not verified	SPR (publisher compound table header) Kd =0.11 ± 0.07 µMpublished ± statistic not independently confirmed · Replicate information not extracted	10.1021/acs.jmedchem.6c00983Publisher source compound table row 23; SPR Kd (µM) columnaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified. Original article table lacks complete assay condition and tested construct details
SYB11parent graph verified assayed material unresolved	NSD3 — PWWP instance unresolvedConstruct not verified	SPR (publisher compound table header) Kd =0.15 ± 0.03 µMpublished ± statistic not independently confirmed · Replicate information not extracted	10.1021/acs.jmedchem.6c00983Publisher source compound table row 24; SPR Kd (µM) columnaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified. Original article table lacks complete assay condition and tested construct details
SYC1parent graph verified assayed material unresolved	NSD3 — PWWP instance unresolvedConstruct not verified	SPR (publisher compound table header) Kd =0.13 ± 0.04 µMpublished ± statistic not independently confirmed · Replicate information not extracted	10.1021/acs.jmedchem.6c00983Publisher source compound table row 27; SPR Kd (µM) columnaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified. Original article table lacks complete assay condition and tested construct details
SYC3parent graph verified assayed material unresolved	NSD3 — PWWP instance unresolvedConstruct not verified	SPR (publisher compound table header) Kd =0.32 ± 0.11 µMpublished ± statistic not independently confirmed · Replicate information not extracted	10.1021/acs.jmedchem.6c00983Publisher source compound table row 29; SPR Kd (µM) columnaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified. Original article table lacks complete assay condition and tested construct details
SYC5parent graph verified assayed material unresolved	NSD3 — PWWP instance unresolvedConstruct not verified	SPR (publisher compound table header) Kd =0.18 ± 0.03 µMpublished ± statistic not independently confirmed · Replicate information not extracted	10.1021/acs.jmedchem.6c00983Publisher source compound table row 31; SPR Kd (µM) columnaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified. Original article table lacks complete assay condition and tested construct details
SYC6parent graph verified assayed material unresolved	NSD3 — PWWP instance unresolvedConstruct not verified	SPR (publisher compound table header) Kd =0.25 ± 0.06 µMpublished ± statistic not independently confirmed · Replicate information not extracted	10.1021/acs.jmedchem.6c00983Publisher source compound table row 32; SPR Kd (µM) columnaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified. Original article table lacks complete assay condition and tested construct details
SYC7parent graph verified assayed material unresolved	NSD3 — PWWP instance unresolvedConstruct not verified	SPR (publisher compound table header) Kd =7.69 ± 0.32 µMpublished ± statistic not independently confirmed · Replicate information not extracted	10.1021/acs.jmedchem.6c00983Publisher source compound table row 33; SPR Kd (µM) columnaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified. Original article table lacks complete assay condition and tested construct details

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