Primary-source preparation · Phase 6B
UNC10088
published name only exact assayed identity unresolved. The tested preparation is distinct from any structural chemical component.
2 experimental observations
Every row retains its original source conditions
| Target scope | Endpoint | Construct, method, conditions | Primary source location |
|---|---|---|---|
| domain specific UP:O96028:PWWP1 | IC50 =248 ± 22 nM SEM | TR-FRET competitive displacement of biotinylated UNC7096 His-tagged NSD2 PWWP1; exact sequence boundaries not independently extracted Without FBXO22 FIST domain n=3 independent experiments | Figure 2h and Supplementary Figure 3D The prepared chemical parent is not stereochemistry/batch resolved; IC50 not Kd. Do not count FBXO22-conditional results as independent chemical identities. |
| domain specific UP:O96028:PWWP1 | IC50 =101 ± 8 nM SEM | TR-FRET competitive displacement of biotinylated UNC7096 His-tagged NSD2 PWWP1 + FBXO22 FIST; exact sequence boundaries not independently extracted With wild-type FBXO22 FIST domain; source-reported cooperative factor alpha = 3.3, not 248/101 n=3 independent experiments | Figure 2h, Supplementary Figure 3D and Results Two different experimental contexts for one preparation; do not average or replace one with another. |
Experimental structural relationship
These are experimentally deposited transformed chemical species in ternary protein complexes, and not binary experimental structures containing the unmodified tested parent.
- PDB 29HG · CCD
A1J20· ligand F [author B] · NSD2 chain B · electron microscopy, 4.0 Å — UNC10088-C derivative; not mapped to exact unmodified administered molecule; Not a binary NSD2 PWWP1 ligand pose for the parent preparation. RCSB entry metadata and deposited component/chain listing independently checked; map density not independently inspected
Source-provenance and uncertainty
Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22 (DOI 10.1038/s41467-026-72235-9)
Access level: primary full text selected figures and methods
Unresolved: Article figure-level read is sufficient for stated outcomes; complete supplemental raw/source-data and exact test-material batches not independently verified. Figure 3e/4h uncertainty and n come from the final 2026 journal figure legends; do not substitute a preprint sample count.
- Figure 2h; TR-FRET competition Methods; Supplementary Figure 3D
- Figure 3e
- Figure 4h
- Data availability