{
  "version": "phase6b-1.0.0",
  "research_snapshot": "2026-10-26",
  "acceptance_policy": "Primary full figure/method or publisher original tabulated chemical graph, assay heading, original result and protein context; uncertainties retained. Original 80 assays and 150 candidates not bulk promoted.",
  "sources": [
    {
      "source_id": "P6B:S:AMANN2026",
      "doi": "10.1038/s41467-026-72235-9",
      "pmid": "42026065",
      "url": "https://www.nature.com/articles/s41467-026-72235-9",
      "title": "Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22",
      "year": 2026,
      "source_access": "primary_full_text_selected_figures_and_methods",
      "inspected_locations": [
        "Figure 2h; TR-FRET competition Methods; Supplementary Figure 3D",
        "Figure 3e",
        "Figure 4h",
        "Data availability"
      ],
      "limits": "Article figure-level read is sufficient for stated outcomes; complete supplemental raw/source-data and exact test-material batches not independently verified. Figure 3e/4h uncertainty and n come from the final 2026 journal figure legends; do not substitute a preprint sample count."
    },
    {
      "source_id": "P6B:S:XIU2026",
      "doi": "10.1021/acs.jmedchem.6c00983",
      "pmid": "42503659",
      "url": "https://pubs.acs.org/doi/10.1021/acs.jmedchem.6c00983",
      "title": "AI-Driven Discovery of a Hydrophobic-Tag Degrader Targeting NSD3 for Lung Squamous Carcinoma Therapy",
      "year": 2026,
      "source_access": "publisher_hosted_primary_compound_table_and_abstract",
      "inspected_locations": [
        "Publisher-hosted original compound spreadsheet, header SPR Kd (µM), rows 20, 23, 24, 27–29, 31–33",
        "Publisher scientific article abstract",
        "Supporting Information availability listing"
      ],
      "limits": "Original source table supplies compound name, SMILES, SPR Kd and reported uncertainty/censored sign. Protein construct boundaries, replicate definitions and assay preparation lots/salts remain unverified. Linked docking models are predictions rather than experimentally deposited complexes."
    },
    {
      "source_id": "P6B:S:COLLIE2025",
      "doi": "10.1021/acsmedchemlett.5c00396",
      "pmid": null,
      "url": "https://pubs.acs.org/doi/10.1021/acsmedchemlett.5c00396",
      "title": "Structural and Molecular Insight into the PWWP1 Domain of NSD2 from the Discovery of Novel Binders Via DNA-Encoded Library Screening",
      "year": 2025,
      "source_access": "primary_full_article_published_table_text",
      "inspected_locations": [
        "Table 1, NSD3 (263–398) column and footnote (published PDF page 3)",
        "Results, NSD3 counter-screen (published PDF page 4)",
        "Scheme 2 stereochemical separation"
      ],
      "limits": "Current exercise confirms table-level NSD3 SPR observation values; it does not reassign exact stereoisomers or independently redo every supplementary SPR fit."
    },
    {
      "source_id": "P6B:S:VANTIEGHEM2026",
      "doi": "10.3390/biom16050716",
      "pmid": "42194065",
      "url": "https://www.mdpi.com/2218-273X/16/5/716",
      "title": "High-Affinity Nanobody Against the LEDGF PWWP Domain Inhibits Chromatin Binding In Vitro",
      "year": 2026,
      "source_access": "primary_full_article_table_and_methods",
      "inspected_locations": [
        "Table 1, NbH10 LEDGF and HRP-2 rows",
        "SPR methods",
        "Figure 3 AlphaScreen"
      ],
      "limits": "Nanobody is a protein binder, not a newly discovered small molecule. Previously accepted rows are not duplicated."
    },
    {
      "source_id": "P6B:S:WU2011",
      "doi": "10.1371/journal.pone.0018919",
      "pmid": null,
      "url": "https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0018919",
      "title": "Structural and Histone Binding Ability Characterizations of Human PWWP Domains",
      "year": 2011,
      "source_access": "primary_full_article_table",
      "inspected_locations": [
        "Table 1 H4K20me3/HDGF2 (HDGFL2) fluorescence-polarization and footnote"
      ],
      "limits": "Very weak/unfittable is qualitative; no numeric Kd or assay limit is inferred."
    },
    {
      "source_id": "P6B:S:LY2026",
      "doi": "10.1021/acs.analchem.5c07785",
      "pmid": "41820229",
      "url": "https://pubs.acs.org/doi/full/10.1021/acs.analchem.5c07785",
      "title": "Fluorogenic Ligand Enables Identification of NSD3-Overexpressed Tumors by Targeting the PWWP1 Domain of NSD3",
      "year": 2026,
      "source_access": "primary_publisher_abstract_only",
      "inspected_locations": [
        "Publisher abstract"
      ],
      "limits": "Ligand graph, assayed preparation, exact construct and method beyond affinity reporting not independently extracted; abstract cannot establish complete assay provenance."
    },
    {
      "source_id": "P6B:S:HS132026",
      "doi": "10.1016/j.bmc.2026.118751",
      "pmid": "42442058",
      "url": "https://www.sciencedirect.com/science/article/pii/S0968089626002075",
      "title": "Discovery and biological evaluation of HS13 as a novel covalent ligand targeting the NSD2-PWWP1 domain",
      "year": 2026,
      "source_access": "publisher_abstract_and_article_preview",
      "inspected_locations": [
        "Published abstract",
        "Publisher article preview Results excerpt"
      ],
      "limits": "Original Phase 5 PWWP:ASSAY:0035 already records this source as a qualitative association. Kd 8.33 µM and IC50 21.62 µM are author-reported but not yet independent method/identity-verified. Publisher abstract says 968 library compounds, extracted Results says 943; preserve discrepancy."
    }
  ],
  "identities": [
    {
      "chemical_id": "INCHIKEY:RXBCCPFICHOEAD-UHFFFAOYSA-N",
      "name": "SYC2",
      "inchikey": "RXBCCPFICHOEAD-UHFFFAOYSA-N",
      "smiles_reported": "FC1=CC(NC2=C(N=C3C=CC(CN)=CN32)C4=CC=C(Cl)C=C4)=CN=C1",
      "identity_scope": "source_reported_parent_connectivity_only",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 28",
      "stereochemistry": "no specified stereocenters in published connectivity",
      "preparation_uncertainty": "No independent salt/solvate/assayed batch confirmation",
      "identity_provenance": "Exact published molecular graph calculated independently with RDKit; does not establish assayed composition"
    },
    {
      "chemical_id": "INCHIKEY:DOSJMSHGMVSFEH-UHFFFAOYSA-N",
      "name": "SYB7",
      "inchikey": "DOSJMSHGMVSFEH-UHFFFAOYSA-N",
      "smiles_reported": "FC1=CC(NC2=C(N=C3C=CC(C(O)=O)=CN32)C4=CC=CC=C4)=CN=C1",
      "identity_scope": "source_reported_parent_connectivity_only",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 20",
      "stereochemistry": "no specified stereocenters in published connectivity",
      "preparation_uncertainty": "Actual assayed salt/solvate/batch has not been independently confirmed",
      "identity_provenance": "Original publisher scientific article table SMILES, RDKit-parsed and independent InChIKey; tested preparation remains distinct"
    },
    {
      "chemical_id": "INCHIKEY:OLIAIHCMUGPGGD-UHFFFAOYSA-N",
      "name": "SYB10",
      "inchikey": "OLIAIHCMUGPGGD-UHFFFAOYSA-N",
      "smiles_reported": "FC1=CC(NC2=C(N=C3C=CC(CN)=CN32)C4=CC=CC=C4)=CN=C1",
      "identity_scope": "source_reported_parent_connectivity_only",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 23",
      "stereochemistry": "no specified stereocenters in published connectivity",
      "preparation_uncertainty": "Actual assayed salt/solvate/batch has not been independently confirmed",
      "identity_provenance": "Original publisher scientific article table SMILES, RDKit-parsed and independent InChIKey; tested preparation remains distinct"
    },
    {
      "chemical_id": "INCHIKEY:XZKIJQSPRFYBOI-UHFFFAOYSA-N",
      "name": "SYB11",
      "inchikey": "XZKIJQSPRFYBOI-UHFFFAOYSA-N",
      "smiles_reported": "FC1=CC(NC2=C(N=C3C=CC(CNC(OC(C)(C)C)=O)=CN32)C4=CC=CC=C4)=CN=C1",
      "identity_scope": "source_reported_parent_connectivity_only",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 24",
      "stereochemistry": "no specified stereocenters in published connectivity",
      "preparation_uncertainty": "Actual assayed salt/solvate/batch has not been independently confirmed",
      "identity_provenance": "Original publisher scientific article table SMILES, RDKit-parsed and independent InChIKey; tested preparation remains distinct"
    },
    {
      "chemical_id": "INCHIKEY:XCNXLNTZUWNKKG-UHFFFAOYSA-N",
      "name": "SYC1",
      "inchikey": "XCNXLNTZUWNKKG-UHFFFAOYSA-N",
      "smiles_reported": "FC1=CC(NC2=C(N=C3C=CC(CN)=CN32)C4=CC=C(C)C=C4)=CN=C1",
      "identity_scope": "source_reported_parent_connectivity_only",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 27",
      "stereochemistry": "no specified stereocenters in published connectivity",
      "preparation_uncertainty": "Actual assayed salt/solvate/batch has not been independently confirmed",
      "identity_provenance": "Original publisher scientific article table SMILES, RDKit-parsed and independent InChIKey; tested preparation remains distinct"
    },
    {
      "chemical_id": "INCHIKEY:HKDFKCWQKXAVDP-UHFFFAOYSA-N",
      "name": "SYC3",
      "inchikey": "HKDFKCWQKXAVDP-UHFFFAOYSA-N",
      "smiles_reported": "FC1=CC(NC2=C(N=C3C=CC(CN)=CN32)C4=CC=CC(Cl)=C4)=CN=C1",
      "identity_scope": "source_reported_parent_connectivity_only",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 29",
      "stereochemistry": "no specified stereocenters in published connectivity",
      "preparation_uncertainty": "Actual assayed salt/solvate/batch has not been independently confirmed",
      "identity_provenance": "Original publisher scientific article table SMILES, RDKit-parsed and independent InChIKey; tested preparation remains distinct"
    },
    {
      "chemical_id": "INCHIKEY:POPGPJNGJZWXMI-UHFFFAOYSA-N",
      "name": "SYC5",
      "inchikey": "POPGPJNGJZWXMI-UHFFFAOYSA-N",
      "smiles_reported": "FC1=CC(NC2=C(N=C3C=CC(CN)=CN32)C4=CC=C(F)C=C4)=CN=C1",
      "identity_scope": "source_reported_parent_connectivity_only",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 31",
      "stereochemistry": "no specified stereocenters in published connectivity",
      "preparation_uncertainty": "Actual assayed salt/solvate/batch has not been independently confirmed",
      "identity_provenance": "Original publisher scientific article table SMILES, RDKit-parsed and independent InChIKey; tested preparation remains distinct"
    },
    {
      "chemical_id": "INCHIKEY:FATGNMUUDUSRIA-UHFFFAOYSA-N",
      "name": "SYC6",
      "inchikey": "FATGNMUUDUSRIA-UHFFFAOYSA-N",
      "smiles_reported": "FC1=CC(NC2=C(N=C3C=CC(CN)=CN32)C4=CC=C(Br)C=C4)=CN=C1",
      "identity_scope": "source_reported_parent_connectivity_only",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 32",
      "stereochemistry": "no specified stereocenters in published connectivity",
      "preparation_uncertainty": "Actual assayed salt/solvate/batch has not been independently confirmed",
      "identity_provenance": "Original publisher scientific article table SMILES, RDKit-parsed and independent InChIKey; tested preparation remains distinct"
    },
    {
      "chemical_id": "INCHIKEY:MDKWQMVHPQBHHH-UHFFFAOYSA-N",
      "name": "SYC7",
      "inchikey": "MDKWQMVHPQBHHH-UHFFFAOYSA-N",
      "smiles_reported": "FC1=CC(NC2=C(N=C3C=CC(CN)=CN32)C4=CC=CC(Br)=C4)=CN=C1",
      "identity_scope": "source_reported_parent_connectivity_only",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 33",
      "stereochemistry": "no specified stereocenters in published connectivity",
      "preparation_uncertainty": "Actual assayed salt/solvate/batch has not been independently confirmed",
      "identity_provenance": "Original publisher scientific article table SMILES, RDKit-parsed and independent InChIKey; tested preparation remains distinct"
    }
  ],
  "preparations": [
    {
      "preparation_id": "P6B:PREP:UNC10088",
      "profile_id": "P6B:PREP:UNC10088",
      "name": "UNC10088",
      "chemical_id": null,
      "source_id": "P6B:S:AMANN2026",
      "identity_status": "published_name_only_exact_assayed_identity_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:UNC10415667",
      "profile_id": "P6B:PREP:UNC10415667",
      "name": "UNC10415667",
      "chemical_id": null,
      "source_id": "P6B:S:AMANN2026",
      "identity_status": "published_name_only_exact_assayed_identity_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:UNC12149",
      "profile_id": "P6B:PREP:UNC12149",
      "name": "UNC12149",
      "chemical_id": null,
      "source_id": "P6B:S:AMANN2026",
      "identity_status": "published_name_only_exact_assayed_identity_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:SYC2",
      "profile_id": "INCHIKEY:RXBCCPFICHOEAD-UHFFFAOYSA-N",
      "name": "SYC2",
      "chemical_id": "INCHIKEY:RXBCCPFICHOEAD-UHFFFAOYSA-N",
      "source_id": "P6B:S:XIU2026",
      "identity_status": "parent_graph_verified_assayed_material_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:SYB7",
      "profile_id": "INCHIKEY:DOSJMSHGMVSFEH-UHFFFAOYSA-N",
      "name": "SYB7",
      "chemical_id": "INCHIKEY:DOSJMSHGMVSFEH-UHFFFAOYSA-N",
      "source_id": "P6B:S:XIU2026",
      "identity_status": "parent_graph_verified_assayed_material_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:SYB10",
      "profile_id": "INCHIKEY:OLIAIHCMUGPGGD-UHFFFAOYSA-N",
      "name": "SYB10",
      "chemical_id": "INCHIKEY:OLIAIHCMUGPGGD-UHFFFAOYSA-N",
      "source_id": "P6B:S:XIU2026",
      "identity_status": "parent_graph_verified_assayed_material_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:SYB11",
      "profile_id": "INCHIKEY:XZKIJQSPRFYBOI-UHFFFAOYSA-N",
      "name": "SYB11",
      "chemical_id": "INCHIKEY:XZKIJQSPRFYBOI-UHFFFAOYSA-N",
      "source_id": "P6B:S:XIU2026",
      "identity_status": "parent_graph_verified_assayed_material_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:SYC1",
      "profile_id": "INCHIKEY:XCNXLNTZUWNKKG-UHFFFAOYSA-N",
      "name": "SYC1",
      "chemical_id": "INCHIKEY:XCNXLNTZUWNKKG-UHFFFAOYSA-N",
      "source_id": "P6B:S:XIU2026",
      "identity_status": "parent_graph_verified_assayed_material_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:SYC3",
      "profile_id": "INCHIKEY:HKDFKCWQKXAVDP-UHFFFAOYSA-N",
      "name": "SYC3",
      "chemical_id": "INCHIKEY:HKDFKCWQKXAVDP-UHFFFAOYSA-N",
      "source_id": "P6B:S:XIU2026",
      "identity_status": "parent_graph_verified_assayed_material_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:SYC5",
      "profile_id": "INCHIKEY:POPGPJNGJZWXMI-UHFFFAOYSA-N",
      "name": "SYC5",
      "chemical_id": "INCHIKEY:POPGPJNGJZWXMI-UHFFFAOYSA-N",
      "source_id": "P6B:S:XIU2026",
      "identity_status": "parent_graph_verified_assayed_material_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:SYC6",
      "profile_id": "INCHIKEY:FATGNMUUDUSRIA-UHFFFAOYSA-N",
      "name": "SYC6",
      "chemical_id": "INCHIKEY:FATGNMUUDUSRIA-UHFFFAOYSA-N",
      "source_id": "P6B:S:XIU2026",
      "identity_status": "parent_graph_verified_assayed_material_unresolved"
    },
    {
      "preparation_id": "P6B:PREP:SYC7",
      "profile_id": "INCHIKEY:MDKWQMVHPQBHHH-UHFFFAOYSA-N",
      "name": "SYC7",
      "chemical_id": "INCHIKEY:MDKWQMVHPQBHHH-UHFFFAOYSA-N",
      "source_id": "P6B:S:XIU2026",
      "identity_status": "parent_graph_verified_assayed_material_unresolved"
    }
  ],
  "accepted_observations": [
    {
      "observation_id": "P6B:ASSAY:0001",
      "preparation_id": "P6B:PREP:UNC10088",
      "protein_id": "UP:O96028",
      "domain_id": "UP:O96028:PWWP1",
      "target_scope": "domain_specific",
      "tested_construct": "His-tagged NSD2 PWWP1; exact sequence boundaries not independently extracted",
      "variant": "wild type not separately certified",
      "method": "TR-FRET competitive displacement of biotinylated UNC7096",
      "endpoint": "IC50",
      "relation": "=",
      "value": 248,
      "units": "nM",
      "uncertainty": 22,
      "uncertainty_type": "SEM",
      "replicates": "n=3 independent experiments",
      "evidence_type": "biochemical_competition",
      "positive_binding_interpretation": "positive competitive engagement; not an equilibrium Kd",
      "conditions": "Without FBXO22 FIST domain",
      "source_id": "P6B:S:AMANN2026",
      "source_location": "Figure 2h and Supplementary Figure 3D",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "The prepared chemical parent is not stereochemistry/batch resolved; IC50 not Kd. Do not count FBXO22-conditional results as independent chemical identities."
    },
    {
      "observation_id": "P6B:ASSAY:0002",
      "preparation_id": "P6B:PREP:UNC10088",
      "protein_id": "UP:O96028",
      "domain_id": "UP:O96028:PWWP1",
      "target_scope": "domain_specific",
      "tested_construct": "His-tagged NSD2 PWWP1 + FBXO22 FIST; exact sequence boundaries not independently extracted",
      "variant": "wild-type FBXO22 FIST; NSD2 variant not specified",
      "method": "TR-FRET competitive displacement of biotinylated UNC7096",
      "endpoint": "IC50",
      "relation": "=",
      "value": 101,
      "units": "nM",
      "uncertainty": 8,
      "uncertainty_type": "SEM",
      "replicates": "n=3 independent experiments",
      "evidence_type": "biochemical_competition",
      "positive_binding_interpretation": "positive cooperative engagement in a ternary context; not an equilibrium Kd",
      "conditions": "With wild-type FBXO22 FIST domain; source-reported cooperative factor alpha = 3.3, not 248/101",
      "source_id": "P6B:S:AMANN2026",
      "source_location": "Figure 2h, Supplementary Figure 3D and Results",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Two different experimental contexts for one preparation; do not average or replace one with another."
    },
    {
      "observation_id": "P6B:ASSAY:0003",
      "preparation_id": "P6B:PREP:UNC10415667",
      "protein_id": "UP:O96028",
      "domain_id": null,
      "target_scope": "protein_level_cellular",
      "tested_construct": "U2OS NSD2-HiBiT cells",
      "variant": "not stated",
      "method": "Cellular HiBiT protein-degradation dose response",
      "endpoint": "DC50",
      "relation": "=",
      "value": 460,
      "units": "nM",
      "uncertainty": null,
      "uncertainty_type": "figure caption reports SEM but numeric uncertainty was not independently extracted",
      "replicates": "n=2 independent experiments (final 2026 article)",
      "evidence_type": "protein_degradation",
      "positive_binding_interpretation": "cellular whole-protein depletion; no isolated-domain affinity",
      "conditions": "U2OS NSD2 HiBiT dose response",
      "source_id": "P6B:S:AMANN2026",
      "source_location": "Figure 3e legend and Results",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Do not copy this whole-protein DC50 to NSD2 PWWP1. 75% at 1 µM for 6 hours is a separate endpoint, not the same dose-response value."
    },
    {
      "observation_id": "P6B:ASSAY:0004",
      "preparation_id": "P6B:PREP:UNC12149",
      "protein_id": "UP:O96028",
      "domain_id": null,
      "target_scope": "protein_level_cellular",
      "tested_construct": "U2OS NSD2-HiBiT cells",
      "variant": "not stated",
      "method": "Cellular HiBiT protein-degradation dose response",
      "endpoint": "DC50",
      "relation": "=",
      "value": 120,
      "units": "nM",
      "uncertainty": null,
      "uncertainty_type": "figure caption reports SEM but numeric uncertainty was not independently extracted",
      "replicates": "n=2 independent experiments (final 2026 article)",
      "evidence_type": "protein_degradation",
      "positive_binding_interpretation": "cellular whole-protein depletion; no isolated-domain affinity",
      "conditions": "U2OS NSD2 HiBiT dose response",
      "source_id": "P6B:S:AMANN2026",
      "source_location": "Figure 4h legend and Results",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Do not transfer cellular DC50 to a particular PWWP-domain biochemical binding constant."
    },
    {
      "observation_id": "P6B:ASSAY:0005",
      "preparation_id": "P6B:PREP:SYC2",
      "protein_id": "UP:Q9BZ95",
      "domain_id": null,
      "target_scope": "NSD3_PWWP_instance_unresolved",
      "tested_construct": null,
      "variant": null,
      "method": "SPR (publisher compound table header)",
      "endpoint": "Kd",
      "relation": "=",
      "value": 0.07,
      "units": "µM",
      "uncertainty": 0.01,
      "uncertainty_type": "published ± statistic not independently confirmed",
      "replicates": null,
      "evidence_type": "source_reported_binding",
      "positive_binding_interpretation": "reported PWWP affinity, but PWWP1 vs PWWP2 attribution remains unverified",
      "conditions": null,
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher compound table row 28; SPR Kd (µM) column, corroborated by abstract",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Published SMILES and SPR column verified; original construct, statistic definition, replicate count and tested lot/salt remain unresolved. Not assignable to NSD3 PWWP1/PWWP2."
    },
    {
      "observation_id": "P6B:ASSAY:0006",
      "preparation_id": "P6B:PREP:SYB7",
      "protein_id": "UP:Q9BZ95",
      "domain_id": null,
      "target_scope": "NSD3_PWWP_instance_unresolved",
      "tested_construct": null,
      "variant": null,
      "method": "SPR (publisher compound table header)",
      "endpoint": "Kd",
      "relation": ">",
      "value": 20.0,
      "units": "µM",
      "uncertainty": null,
      "uncertainty_type": "censored lower bound, no fitted uncertainty",
      "replicates": null,
      "evidence_type": "source_reported_negative_binding",
      "positive_binding_interpretation": "Source reports Kd >20 µM; do NOT interpret as exact Kd, positive binder or categorical inactivity",
      "conditions": "Original article table lacks complete assay condition and tested construct details",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher source compound table row 20; SPR Kd (µM) column",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Censored lower bound only; no numeric exact Kd or biological inactivity conclusion."
    },
    {
      "observation_id": "P6B:ASSAY:0007",
      "preparation_id": "P6B:PREP:SYB10",
      "protein_id": "UP:Q9BZ95",
      "domain_id": null,
      "target_scope": "NSD3_PWWP_instance_unresolved",
      "tested_construct": null,
      "variant": null,
      "method": "SPR (publisher compound table header)",
      "endpoint": "Kd",
      "relation": "=",
      "value": 0.11,
      "units": "µM",
      "uncertainty": 0.07,
      "uncertainty_type": "published ± statistic not independently confirmed",
      "replicates": null,
      "evidence_type": "source_reported_binding",
      "positive_binding_interpretation": "Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established",
      "conditions": "Original article table lacks complete assay condition and tested construct details",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher source compound table row 23; SPR Kd (µM) column",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified."
    },
    {
      "observation_id": "P6B:ASSAY:0008",
      "preparation_id": "P6B:PREP:SYB11",
      "protein_id": "UP:Q9BZ95",
      "domain_id": null,
      "target_scope": "NSD3_PWWP_instance_unresolved",
      "tested_construct": null,
      "variant": null,
      "method": "SPR (publisher compound table header)",
      "endpoint": "Kd",
      "relation": "=",
      "value": 0.15,
      "units": "µM",
      "uncertainty": 0.03,
      "uncertainty_type": "published ± statistic not independently confirmed",
      "replicates": null,
      "evidence_type": "source_reported_binding",
      "positive_binding_interpretation": "Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established",
      "conditions": "Original article table lacks complete assay condition and tested construct details",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher source compound table row 24; SPR Kd (µM) column",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified."
    },
    {
      "observation_id": "P6B:ASSAY:0009",
      "preparation_id": "P6B:PREP:SYC1",
      "protein_id": "UP:Q9BZ95",
      "domain_id": null,
      "target_scope": "NSD3_PWWP_instance_unresolved",
      "tested_construct": null,
      "variant": null,
      "method": "SPR (publisher compound table header)",
      "endpoint": "Kd",
      "relation": "=",
      "value": 0.13,
      "units": "µM",
      "uncertainty": 0.04,
      "uncertainty_type": "published ± statistic not independently confirmed",
      "replicates": null,
      "evidence_type": "source_reported_binding",
      "positive_binding_interpretation": "Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established",
      "conditions": "Original article table lacks complete assay condition and tested construct details",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher source compound table row 27; SPR Kd (µM) column",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified."
    },
    {
      "observation_id": "P6B:ASSAY:0010",
      "preparation_id": "P6B:PREP:SYC3",
      "protein_id": "UP:Q9BZ95",
      "domain_id": null,
      "target_scope": "NSD3_PWWP_instance_unresolved",
      "tested_construct": null,
      "variant": null,
      "method": "SPR (publisher compound table header)",
      "endpoint": "Kd",
      "relation": "=",
      "value": 0.32,
      "units": "µM",
      "uncertainty": 0.11,
      "uncertainty_type": "published ± statistic not independently confirmed",
      "replicates": null,
      "evidence_type": "source_reported_binding",
      "positive_binding_interpretation": "Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established",
      "conditions": "Original article table lacks complete assay condition and tested construct details",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher source compound table row 29; SPR Kd (µM) column",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified."
    },
    {
      "observation_id": "P6B:ASSAY:0011",
      "preparation_id": "P6B:PREP:SYC5",
      "protein_id": "UP:Q9BZ95",
      "domain_id": null,
      "target_scope": "NSD3_PWWP_instance_unresolved",
      "tested_construct": null,
      "variant": null,
      "method": "SPR (publisher compound table header)",
      "endpoint": "Kd",
      "relation": "=",
      "value": 0.18,
      "units": "µM",
      "uncertainty": 0.03,
      "uncertainty_type": "published ± statistic not independently confirmed",
      "replicates": null,
      "evidence_type": "source_reported_binding",
      "positive_binding_interpretation": "Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established",
      "conditions": "Original article table lacks complete assay condition and tested construct details",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher source compound table row 31; SPR Kd (µM) column",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified."
    },
    {
      "observation_id": "P6B:ASSAY:0012",
      "preparation_id": "P6B:PREP:SYC6",
      "protein_id": "UP:Q9BZ95",
      "domain_id": null,
      "target_scope": "NSD3_PWWP_instance_unresolved",
      "tested_construct": null,
      "variant": null,
      "method": "SPR (publisher compound table header)",
      "endpoint": "Kd",
      "relation": "=",
      "value": 0.25,
      "units": "µM",
      "uncertainty": 0.06,
      "uncertainty_type": "published ± statistic not independently confirmed",
      "replicates": null,
      "evidence_type": "source_reported_binding",
      "positive_binding_interpretation": "Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established",
      "conditions": "Original article table lacks complete assay condition and tested construct details",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher source compound table row 32; SPR Kd (µM) column",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified."
    },
    {
      "observation_id": "P6B:ASSAY:0013",
      "preparation_id": "P6B:PREP:SYC7",
      "protein_id": "UP:Q9BZ95",
      "domain_id": null,
      "target_scope": "NSD3_PWWP_instance_unresolved",
      "tested_construct": null,
      "variant": null,
      "method": "SPR (publisher compound table header)",
      "endpoint": "Kd",
      "relation": "=",
      "value": 7.69,
      "units": "µM",
      "uncertainty": 0.32,
      "uncertainty_type": "published ± statistic not independently confirmed",
      "replicates": null,
      "evidence_type": "source_reported_binding",
      "positive_binding_interpretation": "Reported SPR PWWP-domain binding; PWWP1/PWWP2 instance and exact construct not independently established",
      "conditions": "Original article table lacks complete assay condition and tested construct details",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher source compound table row 33; SPR Kd (µM) column",
      "verification_status": "accepted_with_bounded_interpretation",
      "limitation": "Binding method supported by publisher table header; experimental sequence/variant, statistic and salt/lot remain uncertain. No experimentally deposited PWWP-bound complex verified."
    }
  ],
  "unpromoted_source_observations": [
    {
      "lead_id": "P6B:LEAD:HS13_KD",
      "name": "HS13",
      "source_id": "P6B:S:HS132026",
      "source_location": "Abstract",
      "target": "NSD2 PWWP1",
      "endpoint": "Kd",
      "relation": "=",
      "value": 8.33,
      "units": "µM",
      "original_observation_id": "PWWP:ASSAY:0035",
      "reason": "Existing qualitative record, exact assay method and chemical preparation remain unresolved; quantitative value kept as source-reported lead, not counted anew"
    },
    {
      "lead_id": "P6B:LEAD:HS13_IC50",
      "name": "HS13",
      "source_id": "P6B:S:HS132026",
      "source_location": "Publisher Results preview",
      "target": "NSD2 PWWP1",
      "endpoint": "IC50",
      "relation": "=",
      "value": 21.62,
      "units": "µM",
      "original_observation_id": "PWWP:ASSAY:0035",
      "reason": "Assay system, construct, and exact chemistry not independently extracted"
    },
    {
      "lead_id": "P6B:LEAD:LY06_KD",
      "name": "LY-06",
      "source_id": "P6B:S:LY2026",
      "source_location": "Publisher abstract",
      "target": "NSD3 PWWP1",
      "endpoint": "Kd",
      "relation": "=",
      "value": 1.69,
      "units": "µM",
      "uncertainty": 0.05,
      "reason": "Published source abstract supports claim; original assay/structure and full chemical identity not independently reconciled"
    },
    {
      "lead_id": "P6B:LEAD:XSY12_DC50",
      "name": "XSY12",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher abstract",
      "target": "cellular NSD3 protein",
      "endpoint": "DC50",
      "relation": "=",
      "value": 2.21,
      "units": "µM",
      "reason": "Cellular degradation only; exact assayed preparation and full method not independently extracted"
    },
    {
      "lead_id": "P6B:LEAD:XSY12_DMAX",
      "name": "XSY12",
      "source_id": "P6B:S:XIU2026",
      "source_location": "Publisher abstract",
      "target": "cellular NSD3 protein",
      "endpoint": "Dmax",
      "relation": "=",
      "value": 80.2,
      "units": "%",
      "reason": "Protein-level degradation magnitude; not an affinity"
    },
    {
      "lead_id": "P6B:LEAD:92B7_IC50",
      "name": "92-B7 (parthenolide)",
      "source_id": "P6B:S:HS132026",
      "source_location": "Publisher abstract",
      "target": "NSD2 PWWP1",
      "endpoint": "IC50",
      "relation": "=",
      "value": 332,
      "units": "µM",
      "reason": "Original assay technique and preparation not independently reconciled; no new accepted profile"
    }
  ],
  "structure_links": [
    {
      "preparation_id": "P6B:PREP:UNC10088",
      "pdb_id": "29HG",
      "structure_relationship": "chemical_transformation_product_in_FBXO22_NSD2_PWWP1_ternary_complex",
      "component": "UNC10088-C derivative; not mapped to exact unmodified administered molecule",
      "source_id": "P6B:S:AMANN2026",
      "verified_status": "source_reported_ternary_deposited",
      "interpretation": "Not a binary NSD2 PWWP1 ligand pose for the parent preparation",
      "deposited_ccd": "A1J20",
      "ligand_label_asym_id": "F",
      "ligand_auth_asym_id": "B",
      "resolution_angstrom": 4.0,
      "method": "ELECTRON MICROSCOPY",
      "target_chain": "B",
      "fbxo22_chain": "A",
      "rcsb_url": "https://www.rcsb.org/structure/29HG",
      "coordinate_evidence": "RCSB deposited ligand listing and entry metadata; author-reported ternary interface",
      "structural_verification_scope": "RCSB entry metadata and deposited component/chain listing independently checked; map density not independently inspected",
      "source_inspected_location": "RCSB PDB 29HG Experimental Data Snapshot; Small Molecules; Macromolecules"
    },
    {
      "preparation_id": "P6B:PREP:UNC10415667",
      "pdb_id": "29HI",
      "structure_relationship": "chemical_transformation_product_in_FBXO22_NSD2_PWWP1_ternary_complex",
      "component": "UNC10415667-C derivative",
      "source_id": "P6B:S:AMANN2026",
      "verified_status": "source_reported_ternary_deposited",
      "interpretation": "Not an experimentally verified binary ligand pose for the unmodified compound",
      "deposited_ccd": "A1J21",
      "ligand_label_asym_id": "F",
      "ligand_auth_asym_id": "A",
      "resolution_angstrom": 5.9,
      "method": "ELECTRON MICROSCOPY",
      "target_chain": "B",
      "fbxo22_chain": "A",
      "rcsb_url": "https://www.rcsb.org/structure/29HI",
      "coordinate_evidence": "RCSB deposited ligand listing and entry metadata; author-reported ternary interface",
      "structural_verification_scope": "RCSB entry metadata and deposited component/chain listing independently checked; map density not independently inspected",
      "source_inspected_location": "RCSB PDB 29HI Experimental Data Snapshot; Small Molecules; Macromolecules"
    }
  ]
}