Primary-source preparation · Phase 6B
UNC12149
published name only exact assayed identity unresolved. The tested preparation is distinct from any structural chemical component.
1 experimental observations
Every row retains its original source conditions
| Target scope | Endpoint | Construct, method, conditions | Primary source location |
|---|---|---|---|
| protein level cellular No exact isolated PWWP-domain instance assigned | DC50 =120 nM figure caption reports SEM but numeric uncertainty was not independently extracted | Cellular HiBiT protein-degradation dose response U2OS NSD2-HiBiT cells U2OS NSD2 HiBiT dose response n=2 independent experiments (final 2026 article) | Figure 4h legend and Results Do not transfer cellular DC50 to a particular PWWP-domain biochemical binding constant. |
Experimental structural relationship
No verified PWWP-bound experimental structure of this preparation was identified in this Phase 6B snapshot. Docked poses and AlphaFold/predicted conformers do not replace deposited experimental coordinates.
Source-provenance and uncertainty
Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22 (DOI 10.1038/s41467-026-72235-9)
Access level: primary full text selected figures and methods
Unresolved: Article figure-level read is sufficient for stated outcomes; complete supplemental raw/source-data and exact test-material batches not independently verified. Figure 3e/4h uncertainty and n come from the final 2026 journal figure legends; do not substitute a preprint sample count.
- Figure 2h; TR-FRET competition Methods; Supplementary Figure 3D
- Figure 3e
- Figure 4h
- Data availability