Primary-source preparation · Phase 6B
UNC10415667
published name only exact assayed identity unresolved. The tested preparation is distinct from any structural chemical component.
1 experimental observations
Every row retains its original source conditions
| Target scope | Endpoint | Construct, method, conditions | Primary source location |
|---|---|---|---|
| protein level cellular No exact isolated PWWP-domain instance assigned | DC50 =460 nM figure caption reports SEM but numeric uncertainty was not independently extracted | Cellular HiBiT protein-degradation dose response U2OS NSD2-HiBiT cells U2OS NSD2 HiBiT dose response n=2 independent experiments (final 2026 article) | Figure 3e legend and Results Do not copy this whole-protein DC50 to NSD2 PWWP1. 75% at 1 µM for 6 hours is a separate endpoint, not the same dose-response value. |
Experimental structural relationship
These are experimentally deposited transformed chemical species in ternary protein complexes, and not binary experimental structures containing the unmodified tested parent.
- PDB 29HI · CCD
A1J21· ligand F [author A] · NSD2 chain B · electron microscopy, 5.9 Å — UNC10415667-C derivative; Not an experimentally verified binary ligand pose for the unmodified compound. RCSB entry metadata and deposited component/chain listing independently checked; map density not independently inspected
Source-provenance and uncertainty
Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22 (DOI 10.1038/s41467-026-72235-9)
Access level: primary full text selected figures and methods
Unresolved: Article figure-level read is sufficient for stated outcomes; complete supplemental raw/source-data and exact test-material batches not independently verified. Figure 3e/4h uncertainty and n come from the final 2026 journal figure legends; do not substitute a preprint sample count.
- Figure 2h; TR-FRET competition Methods; Supplementary Figure 3D
- Figure 3e
- Figure 4h
- Data availability