| MS9715PREP:MS9715 |
NSD3 PWWP1
247–402Homo sapiens |
KdITC Kddirect binding affinity |
= 1.3 ± 0.17 µM1.3 µM · exact unit conversion |
Experimental context- Replicates
- 2 independent measurements
- Uncertainty definition
- SD
- Temperature
- 15 °C
- Buffer
- 50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP
- Conditions
- {'buffer': '50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP', 'compound_syringe_concentration_uM': 200, 'construct': 'NSD3 247–402 in pNICBio2, E. coli expressed', 'first_injection_removed': True, 'model': 'single binding site', 'pH': 6.8, 'protein_concentration_uM': 20, 'temperature_C': 15}
- Qualification
- Primary manuscript and Methods inspected; individual raw titration files not re-fitted. BI-9321 is a distinct study-specific ITC measurement, not the original 2019 SPR result.
|
PMID:34469831 · 2022NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspectedOriginal observation record{
"DMSO_percent": 1,
"assay_construct_end": 402,
"assay_construct_start": 247,
"assay_context": "purified_domain",
"assay_organism": "Homo sapiens",
"assay_record_id": "PWWP5:ASSAY:0002",
"assay_type": "ITC Kd",
"assay_value_transferred_to_modeled_stereoisomers": false,
"buffer": "50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP",
"ccd_id": null,
"chemical_id": "INCHIKEY:RWXCSOWWDBANDF-LPFSXYLXSA-N",
"compound_name": "MS9715",
"conditions": {
"buffer": "50 mM Tris-HCl; 150 mM NaCl; 1% DMSO; 3% NMP",
"compound_syringe_concentration_uM": 200,
"construct": "NSD3 247\u2013402 in pNICBio2, E. coli expressed",
"first_injection_removed": true,
"model": "single binding site",
"pH": 6.8,
"protein_concentration_uM": 20,
"temperature_C": 15
},
"domain": "PWWP1",
"domain_id": "UP:Q9BZ95:PWWP1",
"endpoint": "Kd",
"evidence_class": "direct_binding_affinity",
"evidence_location": "Results and Figure 2B-D; STAR Methods: ITC",
"evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8882712/",
"gene": "NSD3",
"inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
"is_negative": false,
"numeric_transcription_status": "source_preserved",
"numeric_value_inferred_from_structure": false,
"pH": 6.8,
"pdb_id": null,
"preparation_id": "PREP:MS9715",
"protein_id": "UP:Q9BZ95",
"publication_year": 2022,
"reference_id": "PMID:34469831",
"remaining_uncertainty": "Primary manuscript and Methods inspected; individual raw titration files not re-fitted. BI-9321 is a distinct study-specific ITC measurement, not the original 2019 SPR result.",
"replicates": "2 independent measurements",
"retrieval_date": "2026-10-26",
"role": "reported_degrader",
"source_id": "P5:S:XU2022",
"source_reported_uncertainty": "0.17",
"source_reported_units": "\u00b5M",
"source_reported_value": "1.3",
"source_verification_scope": "NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected",
"temperature_C": 15,
"uncertainty": 0.17,
"uncertainty_type": "SD",
"uncertainty_uM": 0.17,
"units": "\u00b5M",
"value": 1.3,
"value_operator": "=",
"value_uM": 1.3,
"verification_level": "primary_source_verified",
"version": "phase5.chemical_curation.1.1.0"
} |
| MS9715PREP:MS9715 |
NSD3 · protein-level evidence
Construct not curatedHomo sapiens |
DC50Immunoblot degradationcellular protein degradation |
= 4.9 ± 0.4 µM4.9 µM · exact unit conversion |
Experimental context- Replicates
- 3 independent experiments
- Uncertainty definition
- SD
- Temperature
- °C
- Buffer
- Not curated
- Conditions
- {'cell_line': 'MOLM-13', 'duration_hours': 48, 'measured_species': 'NSD3S'}
- Qualification
- Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.
|
PMID:34469831 · 2022NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspectedOriginal observation record{
"assay_construct_end": null,
"assay_construct_start": null,
"assay_context": "cellular_full_protein",
"assay_organism": "Homo sapiens",
"assay_record_id": "PWWP5:ASSAY:0004",
"assay_type": "Immunoblot degradation",
"assay_value_transferred_to_modeled_stereoisomers": false,
"ccd_id": null,
"chemical_id": "INCHIKEY:RWXCSOWWDBANDF-LPFSXYLXSA-N",
"compound_name": "MS9715",
"conditions": {
"cell_line": "MOLM-13",
"duration_hours": 48,
"measured_species": "NSD3S"
},
"domain": null,
"domain_id": null,
"endpoint": "DC50",
"evidence_class": "cellular_protein_degradation",
"evidence_location": "Results; Figure 3E and S1E",
"evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8882712/",
"gene": "NSD3",
"inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
"is_negative": false,
"numeric_transcription_status": "source_preserved",
"numeric_value_inferred_from_structure": false,
"pdb_id": null,
"preparation_id": "PREP:MS9715",
"protein_id": "UP:Q9BZ95",
"publication_year": 2022,
"reference_id": "PMID:34469831",
"remaining_uncertainty": "Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.",
"replicates": "3 independent experiments",
"retrieval_date": "2026-10-26",
"role": "reported_degrader",
"source_id": "P5:S:XU2022",
"source_reported_uncertainty": "0.4",
"source_reported_units": "\u00b5M",
"source_reported_value": "4.9",
"source_verification_scope": "NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected",
"uncertainty": 0.4,
"uncertainty_type": "SD",
"uncertainty_uM": 0.4,
"units": "\u00b5M",
"value": 4.9,
"value_operator": "=",
"value_uM": 4.9,
"verification_level": "primary_source_verified",
"version": "phase5.chemical_curation.1.1.0"
} |
| MS9715PREP:MS9715 |
NSD3 · protein-level evidence
Construct not curatedHomo sapiens |
DmaxImmunoblot degradationcellular protein degradation |
> 80 percent |
Experimental context- Replicates
- 3 independent experiments
- Uncertainty definition
- Not curated; do not assume SD or SEM
- Temperature
- °C
- Buffer
- Not curated
- Conditions
- {'cell_line': 'MOLM-13', 'duration_hours': 48, 'measured_species': 'NSD3S'}
- Qualification
- Main text reports greater than 80%; inequality preserved, no exact Dmax assigned.
|
PMID:34469831 · 2022NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspectedOriginal observation record{
"assay_construct_end": null,
"assay_construct_start": null,
"assay_context": "cellular_full_protein",
"assay_organism": "Homo sapiens",
"assay_record_id": "PWWP5:ASSAY:0005",
"assay_type": "Immunoblot degradation",
"assay_value_transferred_to_modeled_stereoisomers": false,
"ccd_id": null,
"chemical_id": "INCHIKEY:RWXCSOWWDBANDF-LPFSXYLXSA-N",
"compound_name": "MS9715",
"conditions": {
"cell_line": "MOLM-13",
"duration_hours": 48,
"measured_species": "NSD3S"
},
"domain": null,
"domain_id": null,
"endpoint": "Dmax",
"evidence_class": "cellular_protein_degradation",
"evidence_location": "Results: MS9715 effectively degrades NSD3",
"evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8882712/",
"gene": "NSD3",
"inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
"is_negative": false,
"numeric_transcription_status": "source_preserved",
"numeric_value_inferred_from_structure": false,
"pdb_id": null,
"preparation_id": "PREP:MS9715",
"protein_id": "UP:Q9BZ95",
"publication_year": 2022,
"reference_id": "PMID:34469831",
"remaining_uncertainty": "Main text reports greater than 80%; inequality preserved, no exact Dmax assigned.",
"replicates": "3 independent experiments",
"retrieval_date": "2026-10-26",
"role": "reported_degrader",
"source_id": "P5:S:XU2022",
"source_reported_uncertainty": null,
"source_reported_units": "percent",
"source_reported_value": "80",
"source_verification_scope": "NCBI BioC primary full text; Results, ITC Methods and Figure 2/3 captions inspected",
"uncertainty": null,
"uncertainty_type": null,
"uncertainty_uM": null,
"units": "percent",
"value": 80,
"value_operator": "\u003e",
"value_uM": null,
"verification_level": "primary_source_verified",
"version": "phase5.chemical_curation.1.1.0"
} |
