Experimental preparationIdentity not assigned

Measurements remain attached to the reported preparation.

Reported experimental preparation

UNC7753

Studied · no deposited PWWP research ligandPreparation identity unresolved

Named experimental preparation supported by primary publication; no exact molecular depiction assigned until identity is independently resolved.

2 assay / association records1 preparations0 research-ligand PDB entries

No deposited PWWP research-ligand geometry is classified for this preparation in the selected release. Deposition of a buffer/ion is not binding evidence.

Clinical / development status: Not independently curated. A chemical probe classification does not imply medicinal approval.

Identity is separate from preparation

Chemical identity

Measured target and original context

Bioactivity & experimental observations

Download assay CSV

Each row is one source-specific observation. Repeated measurements of this compound are not independent compounds. Direct binding, functional displacement and cellular outcomes retain their distinct endpoints.

Preparation	Tested target & construct	Method / endpoint	Original observation	Conditions & uncertainty	Source & verification
UNC7753PREP:UNC7753	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DC50In-cell western degradationcellular protein degradation	= 1.2 ± 0.3 µM1.2 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS', 'duration_hours': 24, 'readout': 'total NSD2 including major long/short isoforms'} Qualification Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.	PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1 Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0012", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC7753", "conditions": { "cell_line": "U2OS", "duration_hours": 24, "readout": "total NSD2 including major long/short isoforms" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Results; Figure 2A-D", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC7753", "protein_id": "UP:O96028", "publication_year": 2023, "reference_id": "PMID:36976643", "remaining_uncertainty": "Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:HANLEY2023", "source_reported_uncertainty": "0.3", "source_reported_units": "\u00b5M", "source_reported_value": "1.2", "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1", "uncertainty": 0.3, "uncertainty_type": "SEM", "uncertainty_uM": 0.3, "units": "\u00b5M", "value": 1.2, "value_operator": "=", "value_uM": 1.2, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC7753PREP:UNC7753	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DmaxIn-cell western degradationcellular protein degradation	= 81 percent	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS', 'duration_hours': 24} Qualification Main-text 81% transcribed; original numeric figure uncertainty not separately verified, so no uncertainty filled.	PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1 Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0013", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC7753", "conditions": { "cell_line": "U2OS", "duration_hours": 24 }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Results; Figure 2A-D", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC7753", "protein_id": "UP:O96028", "publication_year": 2023, "reference_id": "PMID:36976643", "remaining_uncertainty": "Main-text 81% transcribed; original numeric figure uncertainty not separately verified, so no uncertainty filled.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:HANLEY2023", "source_reported_uncertainty": null, "source_reported_units": "percent", "source_reported_value": "81", "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "percent", "value": 81, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }

Preparation

Tested target & construct

Method / endpoint

Original observation

Conditions & uncertainty

Source & verification

UNC7753PREP:UNC7753

NSD2 · protein-level evidence
Construct not curatedHomo sapiens

DC50In-cell western degradationcellular protein degradation

= 1.2 ± 0.3 µM1.2 µM · exact unit conversion

Experimental context

Replicates: 3 independent experiments
Uncertainty definition: SEM
Temperature: °C
Buffer: Not curated
Conditions: {'cell_line': 'U2OS', 'duration_hours': 24, 'readout': 'total NSD2 including major long/short isoforms'}
Qualification: Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.

PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1

Original observation record

{
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_context": "cellular_full_protein",
  "assay_organism": "Homo sapiens",
  "assay_record_id": "PWWP5:ASSAY:0012",
  "assay_type": "In-cell western degradation",
  "assay_value_transferred_to_modeled_stereoisomers": false,
  "ccd_id": null,
  "chemical_id": null,
  "compound_name": "UNC7753",
  "conditions": {
    "cell_line": "U2OS",
    "duration_hours": 24,
    "readout": "total NSD2 including major long/short isoforms"
  },
  "domain": null,
  "domain_id": null,
  "endpoint": "DC50",
  "evidence_class": "cellular_protein_degradation",
  "evidence_location": "Results; Figure 2A-D",
  "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/",
  "gene": "NSD2",
  "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
  "is_negative": false,
  "numeric_transcription_status": "source_preserved",
  "numeric_value_inferred_from_structure": false,
  "pdb_id": null,
  "preparation_id": "PREP:UNC7753",
  "protein_id": "UP:O96028",
  "publication_year": 2023,
  "reference_id": "PMID:36976643",
  "remaining_uncertainty": "Assay details not explicitly populated remain uncurated; no transfer between assay systems or domains.",
  "replicates": "3 independent experiments",
  "retrieval_date": "2026-10-26",
  "role": "reported_degrader",
  "source_id": "P5:S:HANLEY2023",
  "source_reported_uncertainty": "0.3",
  "source_reported_units": "\u00b5M",
  "source_reported_value": "1.2",
  "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1",
  "uncertainty": 0.3,
  "uncertainty_type": "SEM",
  "uncertainty_uM": 0.3,
  "units": "\u00b5M",
  "value": 1.2,
  "value_operator": "=",
  "value_uM": 1.2,
  "verification_level": "primary_source_verified",
  "version": "phase5.chemical_curation.1.1.0"
}

UNC7753PREP:UNC7753

NSD2 · protein-level evidence
Construct not curatedHomo sapiens

DmaxIn-cell western degradationcellular protein degradation

= 81 percent

Experimental context

Replicates: Not curated
Uncertainty definition: Not curated; do not assume SD or SEM
Temperature: °C
Buffer: Not curated
Conditions: {'cell_line': 'U2OS', 'duration_hours': 24}
Qualification: Main-text 81% transcribed; original numeric figure uncertainty not separately verified, so no uncertainty filled.

PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1

Original observation record

{
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_context": "cellular_full_protein",
  "assay_organism": "Homo sapiens",
  "assay_record_id": "PWWP5:ASSAY:0013",
  "assay_type": "In-cell western degradation",
  "assay_value_transferred_to_modeled_stereoisomers": false,
  "ccd_id": null,
  "chemical_id": null,
  "compound_name": "UNC7753",
  "conditions": {
    "cell_line": "U2OS",
    "duration_hours": 24
  },
  "domain": null,
  "domain_id": null,
  "endpoint": "Dmax",
  "evidence_class": "cellular_protein_degradation",
  "evidence_location": "Results; Figure 2A-D",
  "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/",
  "gene": "NSD2",
  "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
  "is_negative": false,
  "numeric_transcription_status": "source_preserved",
  "numeric_value_inferred_from_structure": false,
  "pdb_id": null,
  "preparation_id": "PREP:UNC7753",
  "protein_id": "UP:O96028",
  "publication_year": 2023,
  "reference_id": "PMID:36976643",
  "remaining_uncertainty": "Main-text 81% transcribed; original numeric figure uncertainty not separately verified, so no uncertainty filled.",
  "replicates": null,
  "retrieval_date": "2026-10-26",
  "role": "reported_degrader",
  "source_id": "P5:S:HANLEY2023",
  "source_reported_uncertainty": null,
  "source_reported_units": "percent",
  "source_reported_value": "81",
  "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1",
  "uncertainty": null,
  "uncertainty_type": null,
  "uncertainty_uM": null,
  "units": "percent",
  "value": 81,
  "value_operator": "=",
  "value_uM": null,
  "verification_level": "primary_source_verified",
  "version": "phase5.chemical_curation.1.1.0"
}

Exact deposited instances

Experimental structures

No verified exact-identity bound structure

The biochemical or cellular evidence remains fully available. No artificial pose has been assigned.

Preparations, identities and controls remain separate

Experimental context

UNC7753

PREP:UNC7753

Named experimental preparation supported by primary publication; no exact molecular depiction assigned until identity is independently resolved.

reported degrader

Complete preparation record

{
  "chemical_id": null,
  "clinical_status": "not independently assessed",
  "curation_version": "phase5-1.0.0",
  "entity_type": "small_molecule_preparation",
  "identity_note": "Named experimental preparation supported by primary publication; no exact molecular depiction assigned until identity is independently resolved.",
  "identity_status": "paper_label_exact_identity_unresolved",
  "molecular_entity_type": "small_molecule_preparation",
  "name": "UNC7753",
  "phase5_identity_overlay": false,
  "preparation_id": "PREP:UNC7753",
  "retrieval_date": "2026-10-26",
  "role": "reported_degrader",
  "source_id": "P5:S:HANLEY2023",
  "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/",
  "version": "phase5.chemical_curation.1.1.0"
}

Source-supported relationships

Relationship	Related identity / preparation	Scope	Source
reported cellular precursor of	UNC8153	Source reports cellular proteolysis; not an interchangeable chemical identity	Source

Provenance and reproducibility

Sources & downloads

https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/

Download complete evidence JSON Curation methods & source receipts

Snapshot 2026-10-26 · phase5-1.0.0 · original measurements, operators, constructs and uncertainty are retained in the exports.