Experimental preparationIdentity not assigned

Measurements remain attached to the reported preparation.

Reported experimental preparation

UNC8325

Studied · no deposited PWWP research ligandPreparation identity unresolved

Named experimental preparation supported by primary publication; no exact molecular depiction assigned until identity is independently resolved.

NSD2 PWWP1

3 assay / association records1 preparations0 research-ligand PDB entries

No deposited PWWP research-ligand geometry is classified for this preparation in the selected release. Deposition of a buffer/ion is not binding evidence.

Clinical / development status: Not independently curated. A chemical probe classification does not imply medicinal approval.

Identity is separate from preparation

Chemical identity

Measured target and original context

Bioactivity & experimental observations

Download assay CSV

Each row is one source-specific observation. Repeated measurements of this compound are not independent compounds. Direct binding, functional displacement and cellular outcomes retain their distinct endpoints.

Preparation	Tested target & construct	Method / endpoint	Original observation	Conditions & uncertainty	Source & verification
UNC8325PREP:UNC8325	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 41 ± 19 nM0.041 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions Not curated Qualification Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0018", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8325", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1; Supplementary Figure 1", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8325", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "19", "source_reported_units": "nM", "source_reported_value": "41", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 19, "uncertainty_type": "SD", "uncertainty_uM": 0.019, "units": "nM", "value": 41, "value_operator": "=", "value_uM": 0.041, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8325PREP:UNC8325	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DmaxIn-cell western degradationcellular protein degradation	= 71 ± 3 percent	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS'} Qualification Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0019", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8325", "conditions": { "cell_line": "U2OS" }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Table 1; Supplementary Figure 2", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8325", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "3", "source_reported_units": "percent", "source_reported_value": "71", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 3, "uncertainty_type": "SD", "uncertainty_uM": null, "units": "percent", "value": 71, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8325PREP:UNC8325	NSD2 · protein-level evidence Construct not curatedHomo sapiens	DC50In-cell western degradationcellular protein degradation	= 0.71 ± 0.25 µM0.71 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions {'cell_line': 'U2OS'} Qualification Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0020", "assay_type": "In-cell western degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8325", "conditions": { "cell_line": "U2OS" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Table 1; Supplementary Figure 2", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8325", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "0.25", "source_reported_units": "\u00b5M", "source_reported_value": "0.71", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 0.25, "uncertainty_type": "SD", "uncertainty_uM": 0.25, "units": "\u00b5M", "value": 0.71, "value_operator": "=", "value_uM": 0.71, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }

Preparation

Tested target & construct

Method / endpoint

Original observation

Conditions & uncertainty

Source & verification

UNC8325PREP:UNC8325

NSD2 PWWP1
Construct not curatedHomo sapiens

KdSPR Kddirect binding affinity

= 41 ± 19 nM0.041 µM · exact unit conversion

Experimental context

Replicates: 3 independent experiments
Uncertainty definition: SD
Temperature: °C
Buffer: Not curated
Conditions: Not curated
Qualification: Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.

PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures

Original observation record

{
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_context": "purified_domain",
  "assay_organism": "Homo sapiens",
  "assay_record_id": "PWWP5:ASSAY:0018",
  "assay_type": "SPR Kd",
  "assay_value_transferred_to_modeled_stereoisomers": false,
  "ccd_id": null,
  "chemical_id": null,
  "compound_name": "UNC8325",
  "conditions": {},
  "domain": "PWWP1",
  "domain_id": "UP:O96028:PWWP1",
  "endpoint": "Kd",
  "evidence_class": "direct_binding_affinity",
  "evidence_location": "Table 1; Supplementary Figure 1",
  "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y",
  "gene": "NSD2",
  "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
  "is_negative": false,
  "numeric_transcription_status": "source_preserved",
  "numeric_value_inferred_from_structure": false,
  "pdb_id": null,
  "preparation_id": "PREP:UNC8325",
  "protein_id": "UP:O96028",
  "publication_year": 2024,
  "reference_id": "PMID:38965384",
  "remaining_uncertainty": "Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.",
  "replicates": "3 independent experiments",
  "retrieval_date": "2026-10-26",
  "role": "reported_degrader",
  "source_id": "P5:S:NIE2024",
  "source_reported_uncertainty": "19",
  "source_reported_units": "nM",
  "source_reported_value": "41",
  "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures",
  "uncertainty": 19,
  "uncertainty_type": "SD",
  "uncertainty_uM": 0.019,
  "units": "nM",
  "value": 41,
  "value_operator": "=",
  "value_uM": 0.041,
  "verification_level": "primary_source_verified",
  "version": "phase5.chemical_curation.1.1.0"
}

UNC8325PREP:UNC8325

NSD2 · protein-level evidence
Construct not curatedHomo sapiens

DmaxIn-cell western degradationcellular protein degradation

= 71 ± 3 percent

Experimental context

Replicates: 3 independent experiments
Uncertainty definition: SD
Temperature: °C
Buffer: Not curated
Conditions: {'cell_line': 'U2OS'}
Qualification: Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.

PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures

Original observation record

{
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_context": "cellular_full_protein",
  "assay_organism": "Homo sapiens",
  "assay_record_id": "PWWP5:ASSAY:0019",
  "assay_type": "In-cell western degradation",
  "assay_value_transferred_to_modeled_stereoisomers": false,
  "ccd_id": null,
  "chemical_id": null,
  "compound_name": "UNC8325",
  "conditions": {
    "cell_line": "U2OS"
  },
  "domain": null,
  "domain_id": null,
  "endpoint": "Dmax",
  "evidence_class": "cellular_protein_degradation",
  "evidence_location": "Table 1; Supplementary Figure 2",
  "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y",
  "gene": "NSD2",
  "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
  "is_negative": false,
  "numeric_transcription_status": "source_preserved",
  "numeric_value_inferred_from_structure": false,
  "pdb_id": null,
  "preparation_id": "PREP:UNC8325",
  "protein_id": "UP:O96028",
  "publication_year": 2024,
  "reference_id": "PMID:38965384",
  "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.",
  "replicates": "3 independent experiments",
  "retrieval_date": "2026-10-26",
  "role": "reported_degrader",
  "source_id": "P5:S:NIE2024",
  "source_reported_uncertainty": "3",
  "source_reported_units": "percent",
  "source_reported_value": "71",
  "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures",
  "uncertainty": 3,
  "uncertainty_type": "SD",
  "uncertainty_uM": null,
  "units": "percent",
  "value": 71,
  "value_operator": "=",
  "value_uM": null,
  "verification_level": "primary_source_verified",
  "version": "phase5.chemical_curation.1.1.0"
}

UNC8325PREP:UNC8325

NSD2 · protein-level evidence
Construct not curatedHomo sapiens

DC50In-cell western degradationcellular protein degradation

= 0.71 ± 0.25 µM0.71 µM · exact unit conversion

Experimental context

Replicates: 3 independent experiments
Uncertainty definition: SD
Temperature: °C
Buffer: Not curated
Conditions: {'cell_line': 'U2OS'}
Qualification: Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.

PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures

Original observation record

{
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_context": "cellular_full_protein",
  "assay_organism": "Homo sapiens",
  "assay_record_id": "PWWP5:ASSAY:0020",
  "assay_type": "In-cell western degradation",
  "assay_value_transferred_to_modeled_stereoisomers": false,
  "ccd_id": null,
  "chemical_id": null,
  "compound_name": "UNC8325",
  "conditions": {
    "cell_line": "U2OS"
  },
  "domain": null,
  "domain_id": null,
  "endpoint": "DC50",
  "evidence_class": "cellular_protein_degradation",
  "evidence_location": "Table 1; Supplementary Figure 2",
  "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y",
  "gene": "NSD2",
  "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
  "is_negative": false,
  "numeric_transcription_status": "source_preserved",
  "numeric_value_inferred_from_structure": false,
  "pdb_id": null,
  "preparation_id": "PREP:UNC8325",
  "protein_id": "UP:O96028",
  "publication_year": 2024,
  "reference_id": "PMID:38965384",
  "remaining_uncertainty": "Cell line confirmed in Supplementary Figure 2; exact duration not filled from a different experiment.",
  "replicates": "3 independent experiments",
  "retrieval_date": "2026-10-26",
  "role": "reported_degrader",
  "source_id": "P5:S:NIE2024",
  "source_reported_uncertainty": "0.25",
  "source_reported_units": "\u00b5M",
  "source_reported_value": "0.71",
  "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures",
  "uncertainty": 0.25,
  "uncertainty_type": "SD",
  "uncertainty_uM": 0.25,
  "units": "\u00b5M",
  "value": 0.71,
  "value_operator": "=",
  "value_uM": 0.71,
  "verification_level": "primary_source_verified",
  "version": "phase5.chemical_curation.1.1.0"
}

Exact deposited instances

Experimental structures

No verified exact-identity bound structure

The biochemical or cellular evidence remains fully available. No artificial pose has been assigned.

Preparations, identities and controls remain separate

Experimental context

UNC8325

PREP:UNC8325

Named experimental preparation supported by primary publication; no exact molecular depiction assigned until identity is independently resolved.

reported degrader

Complete preparation record

{
  "chemical_id": null,
  "clinical_status": "not independently assessed",
  "curation_version": "phase5-1.0.0",
  "entity_type": "small_molecule_preparation",
  "identity_note": "Named experimental preparation supported by primary publication; no exact molecular depiction assigned until identity is independently resolved.",
  "identity_status": "paper_label_exact_identity_unresolved",
  "molecular_entity_type": "small_molecule_preparation",
  "name": "UNC8325",
  "phase5_identity_overlay": false,
  "preparation_id": "PREP:UNC8325",
  "retrieval_date": "2026-10-26",
  "role": "reported_degrader",
  "source_id": "P5:S:NIE2024",
  "source_url": "https://www.nature.com/articles/s41589-024-01660-y",
  "version": "phase5.chemical_curation.1.1.0"
}

Provenance and reproducibility

Sources & downloads

https://www.nature.com/articles/s41589-024-01660-y

Download complete evidence JSON Curation methods & source receipts

Snapshot 2026-10-26 · phase5-1.0.0 · original measurements, operators, constructs and uncertainty are retained in the exports.