Experimental preparationIdentity not assigned

Measurements remain attached to the reported preparation.

Reported experimental preparation

NbH10

Identity / evidence review pendingPreparation identity unresolved

Complete deposited identity retained; no connectivity-only or name-only merge.

3 assay / association records1 preparations0 research-ligand PDB entries

No deposited PWWP research-ligand geometry is classified for this preparation in the selected release. Deposition of a buffer/ion is not binding evidence.

Clinical / development status: Not independently curated. A chemical probe classification does not imply medicinal approval.

Identity is separate from preparation

Chemical identity

Measured target and original context

Bioactivity & experimental observations

Download assay CSV

Each row is one source-specific observation. Repeated measurements of this compound are not independent compounds. Direct binding, functional displacement and cellular outcomes retain their distinct endpoints.

Preparation	Tested target & construct	Method / endpoint	Original observation	Conditions & uncertainty	Source & verification
NbH10PREP:NbH10	PSIP1 · protein-level evidence Construct not curatedVariant: wild type as reportedHomo sapiens	IC50AlphaScreen inhibition IC50full protein chromatin interaction inhibition	= 0.94 ± 0.15 µM0.94 µM · exact unit conversion	Reviewed target attribution: Primary Results explicitly identify full-length LEDGF/p75; Methods specify FLAG-LEDGF/p75 and H3K36me3 nucleosomes. Domain-specific evidence counts must use this reviewed context while preserving the frozen source record. Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {'binding_partner': 'H3K36me3-modified nucleosomes', 'buffer': '25 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM MgCl2; 1 mM DTT; 0.1% Tween 20; 0.1% BSA', 'nucleosome_concentration_nM': 5, 'preincubation': '30 minutes at 4 °C; after nucleosome addition 1 hour at 4 °C; after beads 1 hour at room temperature', 'target': 'full-length FLAG-LEDGF/p75', 'target_concentration_nM': 10} Qualification Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.	PMID:42194065 · 2026local_primary_full_text_XML; Table 1, methods and Results inspected Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0032", "assay_type": "AlphaScreen inhibition IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0032\", \"assay_type\": \"AlphaScreen inhibition IC50\", \"ccd_id\": null, \"compound_name\": \"NbH10\", \"conditions\": {\"partner\": \"H3K36me3 nucleosomes\", \"protein\": \"full-length LEDGF/p75\"}, \"domain\": \"PWWP\", \"evidence_location\": \"Section 3.6; Figure 3\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/\", \"gene\": \"PSIP1\", \"interpretation\": \"Macromolecular binder. NbH10-alone crystal coordinates do not establish a PWWP-bound complex.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:42194065\", \"role\": \"experimentally_studied_macromolecular_binder\", \"uncertainty\": 0.15, \"units\": \"\u00b5M\", \"value\": 0.94, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "named_macromolecule_no_full_sequence_identity", "component_context": null, "compound_name": "NbH10", "conditions": "{\"partner\": \"H3K36me3 nucleosomes\", \"protein\": \"full-length LEDGF/p75\"}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:O75475:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Section 3.6; Figure 3", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/", "gene": "PSIP1", "interpretation": "Macromolecular binder. NbH10-alone crystal coordinates do not establish a PWWP-bound complex.", "molecular_entity_type": "nanobody", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:NbH10", "protein_id": "UP:O75475", "qualitative_result": null, "reference_id": "PMID:42194065", "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.", "replicates": null, "role": "experimentally_studied_macromolecular_binder", "source_component_url": null, "source_id": "S06", "source_reported_uncertainty": "0.15", "source_reported_units": "\u00b5M", "source_reported_value": "0.94", "source_verification_scope": "local_primary_full_text_XML; Table 1, methods and Results inspected", "temperature_C": null, "uncertainty": 0.15, "uncertainty_type": null, "uncertainty_uM": 0.15, "unit_conversion": null, "units": "\u00b5M", "value": 0.94, "value_operator": "=", "value_uM": 0.94, "verification_level": "primary_full_text_sections_verified" }
NbH10PREP:NbH10	PSIP1 PWWP1 1–90Homo sapiens	KdSPR apparent Kddirect binding affinity	= 94.4 ± 9.1 nM0.0944 µM · exact unit conversion	Experimental context Replicates 3 Uncertainty definition SD Temperature 25 °C Buffer 50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20 Conditions {"protein_construct": "PSIP1 1–90", "replicates": 3, "uncertainty_type": "SD"} Qualification Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody–PWWP complex.	PMID:42194065 · 2026local_primary_full_text_XML_table_and_methods_verified Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 90, "assay_construct_start": 1, "assay_record_id": "PWWP11:ASSAY:0008", "assay_type": "SPR apparent Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "named_macromolecule_no_full_sequence_identity", "component_context": null, "compound_name": "NbH10", "conditions": "{\"protein_construct\": \"PSIP1 1\u201390\", \"replicates\": 3, \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O75475:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1 NbH10 column; SPR methods", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/", "gene": "PSIP1", "interpretation": "Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody\u2013PWWP complex.", "molecular_entity_type": "nanobody", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:NbH10", "protein_id": "UP:O75475", "qualitative_result": null, "reference_id": "PMID:42194065", "remaining_uncertainty": null, "replicates": "3", "role": "macromolecular_binder", "source_component_url": null, "source_id": "S06", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified", "temperature_C": 25, "uncertainty": 9.1, "uncertainty_type": "SD", "uncertainty_uM": 0.0091, "unit_conversion": null, "units": "nM", "value": 94.4, "value_operator": "=", "value_uM": 0.0944, "verification_level": null }
NbH10PREP:NbH10	HDGFL2 PWWP1 1–93Homo sapiens	KdSPR apparent Kddirect binding affinity	= 81.2 ± 1.8 nM0.0812 µM · exact unit conversion	Experimental context Replicates 3 Uncertainty definition SD Temperature 25 °C Buffer 50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20 Conditions {"protein_construct": "HDGFL2 1–93", "replicates": 3, "uncertainty_type": "SD"} Qualification Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody–PWWP complex.	PMID:42194065 · 2026local_primary_full_text_XML_table_and_methods_verified Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 93, "assay_construct_start": 1, "assay_record_id": "PWWP11:ASSAY:0009", "assay_type": "SPR apparent Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "named_macromolecule_no_full_sequence_identity", "component_context": null, "compound_name": "NbH10", "conditions": "{\"protein_construct\": \"HDGFL2 1\u201393\", \"replicates\": 3, \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1 NbH10 column; SPR methods", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/", "gene": "HDGFL2", "interpretation": "Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody\u2013PWWP complex.", "molecular_entity_type": "nanobody", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:NbH10", "protein_id": "UP:Q7Z4V5", "qualitative_result": null, "reference_id": "PMID:42194065", "remaining_uncertainty": null, "replicates": "3", "role": "macromolecular_binder", "source_component_url": null, "source_id": "S06", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified", "temperature_C": 25, "uncertainty": 1.8, "uncertainty_type": "SD", "uncertainty_uM": 0.0018, "unit_conversion": null, "units": "nM", "value": 81.2, "value_operator": "=", "value_uM": 0.0812, "verification_level": null }

Preparation

Tested target & construct

Method / endpoint

Original observation

Conditions & uncertainty

Source & verification

NbH10PREP:NbH10

PSIP1 · protein-level evidence
Construct not curatedVariant: wild type as reportedHomo sapiens

IC50AlphaScreen inhibition IC50full protein chromatin interaction inhibition

= 0.94 ± 0.15 µM0.94 µM · exact unit conversion

Reviewed target attribution: Primary Results explicitly identify full-length LEDGF/p75; Methods specify FLAG-LEDGF/p75 and H3K36me3 nucleosomes. Domain-specific evidence counts must use this reviewed context while preserving the frozen source record.

Experimental context

Replicates: Not curated
Uncertainty definition: Not curated; do not assume SD or SEM
Temperature: —
Buffer: Not curated
Conditions: {'binding_partner': 'H3K36me3-modified nucleosomes', 'buffer': '25 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM MgCl2; 1 mM DTT; 0.1% Tween 20; 0.1% BSA', 'nucleosome_concentration_nM': 5, 'preincubation': '30 minutes at 4 °C; after nucleosome addition 1 hour at 4 °C; after beads 1 hour at room temperature', 'target': 'full-length FLAG-LEDGF/p75', 'target_concentration_nM': 10}
Qualification: Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.

PMID:42194065 · 2026local_primary_full_text_XML; Table 1, methods and Results inspected

Original observation record

{
  "DMSO_percent": null,
  "abstract_reported_endpoint": null,
  "abstract_reported_method": null,
  "abstract_reported_units": null,
  "abstract_reported_value": null,
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_record_id": "PWWP:ASSAY:0032",
  "assay_type": "AlphaScreen inhibition IC50",
  "assay_value_transferred_to_modeled_stereoisomers": 0,
  "audit_decision": "retain_with_explicit_endpoint_and_identity",
  "baseline_assay_record": 1,
  "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0032\", \"assay_type\": \"AlphaScreen inhibition IC50\", \"ccd_id\": null, \"compound_name\": \"NbH10\", \"conditions\": {\"partner\": \"H3K36me3 nucleosomes\", \"protein\": \"full-length LEDGF/p75\"}, \"domain\": \"PWWP\", \"evidence_location\": \"Section 3.6; Figure 3\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/\", \"gene\": \"PSIP1\", \"interpretation\": \"Macromolecular binder. NbH10-alone crystal coordinates do not establish a PWWP-bound complex.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:42194065\", \"role\": \"experimentally_studied_macromolecular_binder\", \"uncertainty\": 0.15, \"units\": \"\u00b5M\", \"value\": 0.94, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}",
  "buffer": null,
  "ccd_id": null,
  "ccd_mapping_source": null,
  "chemical_id": null,
  "chemical_identity_status": "named_macromolecule_no_full_sequence_identity",
  "component_context": null,
  "compound_name": "NbH10",
  "conditions": "{\"partner\": \"H3K36me3 nucleosomes\", \"protein\": \"full-length LEDGF/p75\"}",
  "crystallographic_component_ids": null,
  "domain": "PWWP",
  "domain_id": "UP:O75475:PWWP1",
  "endpoint_label_status": null,
  "evidence_class": "biochemical_interaction_inhibition",
  "evidence_location": "Section 3.6; Figure 3",
  "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/",
  "gene": "PSIP1",
  "interpretation": "Macromolecular binder. NbH10-alone crystal coordinates do not establish a PWWP-bound complex.",
  "molecular_entity_type": "nanobody",
  "numeric_transcription_status": "matches_primary_report",
  "numeric_value_inferred_from_structure": 0,
  "pH": null,
  "pdb_id": null,
  "preparation_id": "PREP:NbH10",
  "protein_id": "UP:O75475",
  "qualitative_result": null,
  "reference_id": "PMID:42194065",
  "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.",
  "replicates": null,
  "role": "experimentally_studied_macromolecular_binder",
  "source_component_url": null,
  "source_id": "S06",
  "source_reported_uncertainty": "0.15",
  "source_reported_units": "\u00b5M",
  "source_reported_value": "0.94",
  "source_verification_scope": "local_primary_full_text_XML; Table 1, methods and Results inspected",
  "temperature_C": null,
  "uncertainty": 0.15,
  "uncertainty_type": null,
  "uncertainty_uM": 0.15,
  "unit_conversion": null,
  "units": "\u00b5M",
  "value": 0.94,
  "value_operator": "=",
  "value_uM": 0.94,
  "verification_level": "primary_full_text_sections_verified"
}

NbH10PREP:NbH10

PSIP1 PWWP1
1–90Homo sapiens

KdSPR apparent Kddirect binding affinity

= 94.4 ± 9.1 nM0.0944 µM · exact unit conversion

Experimental context

Replicates: 3
Uncertainty definition: SD
Temperature: 25 °C
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20
Conditions: {"protein_construct": "PSIP1 1–90", "replicates": 3, "uncertainty_type": "SD"}
Qualification: Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody–PWWP complex.

PMID:42194065 · 2026local_primary_full_text_XML_table_and_methods_verified

Original observation record

{
  "DMSO_percent": null,
  "abstract_reported_endpoint": null,
  "abstract_reported_method": null,
  "abstract_reported_units": null,
  "abstract_reported_value": null,
  "assay_construct_end": 90,
  "assay_construct_start": 1,
  "assay_record_id": "PWWP11:ASSAY:0008",
  "assay_type": "SPR apparent Kd",
  "assay_value_transferred_to_modeled_stereoisomers": 0,
  "audit_decision": null,
  "baseline_assay_record": 0,
  "baseline_original": null,
  "buffer": "50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20",
  "ccd_id": null,
  "ccd_mapping_source": null,
  "chemical_id": null,
  "chemical_identity_status": "named_macromolecule_no_full_sequence_identity",
  "component_context": null,
  "compound_name": "NbH10",
  "conditions": "{\"protein_construct\": \"PSIP1 1\u201390\", \"replicates\": 3, \"uncertainty_type\": \"SD\"}",
  "crystallographic_component_ids": null,
  "domain": "PWWP1",
  "domain_id": "UP:O75475:PWWP1",
  "endpoint_label_status": null,
  "evidence_class": "direct_binding_affinity",
  "evidence_location": "Table 1 NbH10 column; SPR methods",
  "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/",
  "gene": "PSIP1",
  "interpretation": "Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody\u2013PWWP complex.",
  "molecular_entity_type": "nanobody",
  "numeric_transcription_status": "matches_primary_table",
  "numeric_value_inferred_from_structure": null,
  "pH": 7.4,
  "pdb_id": null,
  "preparation_id": "PREP:NbH10",
  "protein_id": "UP:O75475",
  "qualitative_result": null,
  "reference_id": "PMID:42194065",
  "remaining_uncertainty": null,
  "replicates": "3",
  "role": "macromolecular_binder",
  "source_component_url": null,
  "source_id": "S06",
  "source_reported_uncertainty": null,
  "source_reported_units": null,
  "source_reported_value": null,
  "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified",
  "temperature_C": 25,
  "uncertainty": 9.1,
  "uncertainty_type": "SD",
  "uncertainty_uM": 0.0091,
  "unit_conversion": null,
  "units": "nM",
  "value": 94.4,
  "value_operator": "=",
  "value_uM": 0.0944,
  "verification_level": null
}

NbH10PREP:NbH10

HDGFL2 PWWP1
1–93Homo sapiens

KdSPR apparent Kddirect binding affinity

= 81.2 ± 1.8 nM0.0812 µM · exact unit conversion

Experimental context

Replicates: 3
Uncertainty definition: SD
Temperature: 25 °C
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20
Conditions: {"protein_construct": "HDGFL2 1–93", "replicates": 3, "uncertainty_type": "SD"}
Qualification: Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody–PWWP complex.

PMID:42194065 · 2026local_primary_full_text_XML_table_and_methods_verified

Original observation record

{
  "DMSO_percent": null,
  "abstract_reported_endpoint": null,
  "abstract_reported_method": null,
  "abstract_reported_units": null,
  "abstract_reported_value": null,
  "assay_construct_end": 93,
  "assay_construct_start": 1,
  "assay_record_id": "PWWP11:ASSAY:0009",
  "assay_type": "SPR apparent Kd",
  "assay_value_transferred_to_modeled_stereoisomers": 0,
  "audit_decision": null,
  "baseline_assay_record": 0,
  "baseline_original": null,
  "buffer": "50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20",
  "ccd_id": null,
  "ccd_mapping_source": null,
  "chemical_id": null,
  "chemical_identity_status": "named_macromolecule_no_full_sequence_identity",
  "component_context": null,
  "compound_name": "NbH10",
  "conditions": "{\"protein_construct\": \"HDGFL2 1\u201393\", \"replicates\": 3, \"uncertainty_type\": \"SD\"}",
  "crystallographic_component_ids": null,
  "domain": "PWWP1",
  "domain_id": "UP:Q7Z4V5:PWWP1",
  "endpoint_label_status": null,
  "evidence_class": "direct_binding_affinity",
  "evidence_location": "Table 1 NbH10 column; SPR methods",
  "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/",
  "gene": "HDGFL2",
  "interpretation": "Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody\u2013PWWP complex.",
  "molecular_entity_type": "nanobody",
  "numeric_transcription_status": "matches_primary_table",
  "numeric_value_inferred_from_structure": null,
  "pH": 7.4,
  "pdb_id": null,
  "preparation_id": "PREP:NbH10",
  "protein_id": "UP:Q7Z4V5",
  "qualitative_result": null,
  "reference_id": "PMID:42194065",
  "remaining_uncertainty": null,
  "replicates": "3",
  "role": "macromolecular_binder",
  "source_component_url": null,
  "source_id": "S06",
  "source_reported_uncertainty": null,
  "source_reported_units": null,
  "source_reported_value": null,
  "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified",
  "temperature_C": 25,
  "uncertainty": 1.8,
  "uncertainty_type": "SD",
  "uncertainty_uM": 0.0018,
  "unit_conversion": null,
  "units": "nM",
  "value": 81.2,
  "value_operator": "=",
  "value_uM": 0.0812,
  "verification_level": null
}

Exact deposited instances

Experimental structures

No verified exact-identity bound structure

The biochemical or cellular evidence remains fully available. No artificial pose has been assigned.

Preparations, identities and controls remain separate

Experimental context

NbH10

PREP:NbH10

Complete deposited identity retained; no connectivity-only or name-only merge.

assay preparation

Complete preparation record

{
  "chemical_id": null,
  "entity_type": "nanobody",
  "exact_ccd_id": null,
  "identity_note": "Complete deposited identity retained; no connectivity-only or name-only merge.",
  "identity_status": "named_macromolecule_no_full_sequence_identity",
  "modeled_candidate_ccd_ids": "[]",
  "modeled_candidate_chemical_ids": "[]",
  "name": "NbH10",
  "preparation_id": "PREP:NbH10",
  "role": "assay_preparation",
  "sequence_if_defined": null
}

Provenance and reproducibility

Sources & downloads

https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/

Download complete evidence JSON Curation methods & source receipts

Snapshot 2026-10-26 · phase5-1.0.0 · original measurements, operators, constructs and uncertainty are retained in the exports.