Experimental preparationIdentity not assigned

Measurements remain attached to the reported preparation.

Reported experimental preparation

compound 1 (de Freitas 2021)

Studied · no deposited PWWP research ligandPreparation identity unresolved

The source describes two stereocenters, one diastereoisomer and equipotent enantiomers. The measured preparation is retained without assigning an exact enantiomeric identity or composition.

NSD2 PWWP1

2 assay / association records1 preparations0 research-ligand PDB entries

No deposited PWWP research-ligand geometry is classified for this preparation in the selected release. Deposition of a buffer/ion is not binding evidence.

Clinical / development status: Not independently curated. A chemical probe classification does not imply medicinal approval.

Identity is separate from preparation

Chemical identity

Measured target and original context

Bioactivity & experimental observations

Download assay CSV

Each row is one source-specific observation. Repeated measurements of this compound are not independent compounds. Direct binding, functional displacement and cellular outcomes retain their distinct endpoints.

Preparation	Tested target & construct	Method / endpoint	Original observation	Conditions & uncertainty	Source & verification
compound 1 (de Freitas 2021)PREP:DEFREITAS2021_1	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 41 ± 8 µM41 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification Source text directly reports preparation and Kd ± uncertainty; supplementary figure and compound-1-specific buffer/construct/statistic not independently recovered. The SPR Methods paragraph explicitly names 3f, so its conditions are not copied to compound 1.	PMID:33522809 · 2021NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0033", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "compound 1 (de Freitas 2021)", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure S1a", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:DEFREITAS2021_1", "protein_id": "UP:O96028", "publication_year": 2021, "reference_id": "PMID:33522809", "remaining_uncertainty": "Source text directly reports preparation and Kd \u00b1 uncertainty; supplementary figure and compound-1-specific buffer/construct/statistic not independently recovered. The SPR Methods paragraph explicitly names 3f, so its conditions are not copied to compound 1.", "replicates": null, "retrieval_date": "2026-10-26", "role": "fragment_hit", "source_id": "P5:S:DEFREITAS2021", "source_reported_uncertainty": "8", "source_reported_units": "\u00b5M", "source_reported_value": "41", "source_verification_scope": "NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved", "uncertainty": 8, "uncertainty_type": null, "uncertainty_uM": 8, "units": "\u00b5M", "value": 41, "value_operator": "=", "value_uM": 41, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
compound 1 (de Freitas 2021)PREP:DEFREITAS2021_1	NSD2 PWWP1 Construct not curatedHomo sapiens	KdReverse ITC Kddirect binding affinity	= 8.9 µM8.9 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'compound_cell_concentration_uM': 40, 'protein_syringe_concentration_mM': 1, 'reverse_titration_reason': 'Limited compound solubility', 'titration_direction': 'Protein into compound'} Qualification No uncertainty, replicate count or exact stereochemical assignment inferred from the primary Results value. A distinct reverse-ITC experiment is retained separately from SPR.	PMID:33522809 · 2021NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0034", "assay_type": "Reverse ITC Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "compound 1 (de Freitas 2021)", "conditions": { "compound_cell_concentration_uM": 40, "protein_syringe_concentration_mM": 1, "reverse_titration_reason": "Limited compound solubility", "titration_direction": "Protein into compound" }, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure S1b", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:DEFREITAS2021_1", "protein_id": "UP:O96028", "publication_year": 2021, "reference_id": "PMID:33522809", "remaining_uncertainty": "No uncertainty, replicate count or exact stereochemical assignment inferred from the primary Results value. A distinct reverse-ITC experiment is retained separately from SPR.", "replicates": null, "retrieval_date": "2026-10-26", "role": "fragment_hit", "source_id": "P5:S:DEFREITAS2021", "source_reported_uncertainty": null, "source_reported_units": "\u00b5M", "source_reported_value": "8.9", "source_verification_scope": "NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "\u00b5M", "value": 8.9, "value_operator": "=", "value_uM": 8.9, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }

Preparation

Tested target & construct

Method / endpoint

Original observation

Conditions & uncertainty

Source & verification

compound 1 (de Freitas 2021)PREP:DEFREITAS2021_1

NSD2 PWWP1
Construct not curatedHomo sapiens

KdSPR Kddirect binding affinity

= 41 ± 8 µM41 µM · exact unit conversion

Experimental context

Replicates: Not curated
Uncertainty definition: Not curated; do not assume SD or SEM
Temperature: °C
Buffer: Not curated
Conditions: Not curated
Qualification: Source text directly reports preparation and Kd ± uncertainty; supplementary figure and compound-1-specific buffer/construct/statistic not independently recovered. The SPR Methods paragraph explicitly names 3f, so its conditions are not copied to compound 1.

PMID:33522809 · 2021NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved

Original observation record

{
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_context": "purified_domain",
  "assay_organism": "Homo sapiens",
  "assay_record_id": "PWWP5:ASSAY:0033",
  "assay_type": "SPR Kd",
  "assay_value_transferred_to_modeled_stereoisomers": false,
  "ccd_id": null,
  "chemical_id": null,
  "compound_name": "compound 1 (de Freitas 2021)",
  "conditions": {},
  "domain": "PWWP1",
  "domain_id": "UP:O96028:PWWP1",
  "endpoint": "Kd",
  "evidence_class": "direct_binding_affinity",
  "evidence_location": "Results; Figure S1a",
  "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/",
  "gene": "NSD2",
  "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
  "is_negative": false,
  "numeric_transcription_status": "source_preserved",
  "numeric_value_inferred_from_structure": false,
  "pdb_id": null,
  "preparation_id": "PREP:DEFREITAS2021_1",
  "protein_id": "UP:O96028",
  "publication_year": 2021,
  "reference_id": "PMID:33522809",
  "remaining_uncertainty": "Source text directly reports preparation and Kd \u00b1 uncertainty; supplementary figure and compound-1-specific buffer/construct/statistic not independently recovered. The SPR Methods paragraph explicitly names 3f, so its conditions are not copied to compound 1.",
  "replicates": null,
  "retrieval_date": "2026-10-26",
  "role": "fragment_hit",
  "source_id": "P5:S:DEFREITAS2021",
  "source_reported_uncertainty": "8",
  "source_reported_units": "\u00b5M",
  "source_reported_value": "41",
  "source_verification_scope": "NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved",
  "uncertainty": 8,
  "uncertainty_type": null,
  "uncertainty_uM": 8,
  "units": "\u00b5M",
  "value": 41,
  "value_operator": "=",
  "value_uM": 41,
  "verification_level": "primary_source_verified",
  "version": "phase5.chemical_curation.1.1.0"
}

compound 1 (de Freitas 2021)PREP:DEFREITAS2021_1

NSD2 PWWP1
Construct not curatedHomo sapiens

KdReverse ITC Kddirect binding affinity

= 8.9 µM8.9 µM · exact unit conversion

Experimental context

Replicates: Not curated
Uncertainty definition: Not curated; do not assume SD or SEM
Temperature: °C
Buffer: Not curated
Conditions: {'compound_cell_concentration_uM': 40, 'protein_syringe_concentration_mM': 1, 'reverse_titration_reason': 'Limited compound solubility', 'titration_direction': 'Protein into compound'}
Qualification: No uncertainty, replicate count or exact stereochemical assignment inferred from the primary Results value. A distinct reverse-ITC experiment is retained separately from SPR.

PMID:33522809 · 2021NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved

Original observation record

{
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_context": "purified_domain",
  "assay_organism": "Homo sapiens",
  "assay_record_id": "PWWP5:ASSAY:0034",
  "assay_type": "Reverse ITC Kd",
  "assay_value_transferred_to_modeled_stereoisomers": false,
  "ccd_id": null,
  "chemical_id": null,
  "compound_name": "compound 1 (de Freitas 2021)",
  "conditions": {
    "compound_cell_concentration_uM": 40,
    "protein_syringe_concentration_mM": 1,
    "reverse_titration_reason": "Limited compound solubility",
    "titration_direction": "Protein into compound"
  },
  "domain": "PWWP1",
  "domain_id": "UP:O96028:PWWP1",
  "endpoint": "Kd",
  "evidence_class": "direct_binding_affinity",
  "evidence_location": "Results; Figure S1b",
  "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/",
  "gene": "NSD2",
  "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.",
  "is_negative": false,
  "numeric_transcription_status": "source_preserved",
  "numeric_value_inferred_from_structure": false,
  "pdb_id": null,
  "preparation_id": "PREP:DEFREITAS2021_1",
  "protein_id": "UP:O96028",
  "publication_year": 2021,
  "reference_id": "PMID:33522809",
  "remaining_uncertainty": "No uncertainty, replicate count or exact stereochemical assignment inferred from the primary Results value. A distinct reverse-ITC experiment is retained separately from SPR.",
  "replicates": null,
  "retrieval_date": "2026-10-26",
  "role": "fragment_hit",
  "source_id": "P5:S:DEFREITAS2021",
  "source_reported_uncertainty": null,
  "source_reported_units": "\u00b5M",
  "source_reported_value": "8.9",
  "source_verification_scope": "NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved",
  "uncertainty": null,
  "uncertainty_type": null,
  "uncertainty_uM": null,
  "units": "\u00b5M",
  "value": 8.9,
  "value_operator": "=",
  "value_uM": 8.9,
  "verification_level": "primary_source_verified",
  "version": "phase5.chemical_curation.1.1.0"
}

Exact deposited instances

Experimental structures

No verified exact-identity bound structure

The biochemical or cellular evidence remains fully available. No artificial pose has been assigned.

Preparations, identities and controls remain separate

Experimental context

compound 1 (de Freitas 2021)

PREP:DEFREITAS2021_1

The source describes two stereocenters, one diastereoisomer and equipotent enantiomers. The measured preparation is retained without assigning an exact enantiomeric identity or composition.

fragment hit

Complete preparation record

{
  "chemical_id": null,
  "clinical_status": "not independently assessed",
  "curation_version": "phase5-1.0.0",
  "entity_type": "small_molecule_preparation",
  "identity_note": "The source describes two stereocenters, one diastereoisomer and equipotent enantiomers. The measured preparation is retained without assigning an exact enantiomeric identity or composition.",
  "identity_status": "paper_label_exact_identity_unresolved",
  "molecular_entity_type": "small_molecule_preparation",
  "name": "compound 1 (de Freitas 2021)",
  "phase5_identity_overlay": false,
  "preparation_id": "PREP:DEFREITAS2021_1",
  "retrieval_date": "2026-10-26",
  "role": "fragment_hit",
  "source_id": "P5:S:DEFREITAS2021",
  "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/",
  "version": "phase5.chemical_curation.1.1.0"
}

Provenance and reproducibility

Sources & downloads

https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/

Download complete evidence JSON Curation methods & source receipts

Snapshot 2026-10-26 · phase5-1.0.0 · original measurements, operators, constructs and uncertainty are retained in the exports.