Scientific evidence classification · Phase 6A preserved + Phase 6B additions

PWWP Compounds
& Bioactivity

Explore experimentally studied preparations separately from deposited research ligands, buffers, ions and unresolved chemical evidence. Every role is source- and target-context specific.

Explore studied compounds Structural & Binding Landscape →

Different denominators · 26 October 2026

38studied preparation profiles · 26 baseline + 12 new

109profiles with PWWP research-ligand geometry

93source assay / association observations · 80 baseline + 13 new

Combined Phase 6A + Phase 6B denominators. These groups overlap; the 109 structurally source-tagged research ligands are unchanged. Deposited components and reagents are not automatically biochemical binders.

Search compounds & identifiersProteinEvidence view

Specific PWWP domainExact PWWP-proximal deposited siteChemical identity

Assay, source & experimental-context filters

Assay method Endpoint Reported relation Exact concentration min (µM)Exact concentration max (µM) Publication / source databasePublication yearAssay organismTested constructVerification status / scope Missing experimental detailAny associated PDB structure Inventory scopeSort

Assay filters must match the same observation. Concentration ranges require one endpoint and exact positive measurements; inequalities and qualitative findings remain searchable separately.

Phase 6B · source-reviewed additions (separate from the Phase 6A snapshot)

13 matching new observations · 12 preparations

Keep source-level IC50, Kd, and whole-protein DC50 separate; these records were not included in the original 80.

Filter all Phase 6B assay contexts

Preparation	Target scope	Original assay	Primary source
UNC10088	NSD2 PWWP1, exact domain	IC50 =248 ± 22 nM TR-FRET competitive displacement of biotinylated UNC7096	10.1038/s41467-026-72235-9 Figure 2h and Supplementary Figure 3D
UNC10088	NSD2 PWWP1, exact domain	IC50 =101 ± 8 nM TR-FRET competitive displacement of biotinylated UNC7096	10.1038/s41467-026-72235-9 Figure 2h, Supplementary Figure 3D and Results
UNC10415667	NSD2 protein, cellular only	DC50 =460 nM Cellular HiBiT protein-degradation dose response	10.1038/s41467-026-72235-9 Figure 3e legend and Results
UNC12149	NSD2 protein, cellular only	DC50 =120 nM Cellular HiBiT protein-degradation dose response	10.1038/s41467-026-72235-9 Figure 4h legend and Results
SYC2	NSD3 PWWP, domain instance unresolved	Kd =0.07 ± 0.01 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher compound table row 28; SPR Kd (µM) column, corroborated by abstract
SYB7	NSD3 PWWP, domain instance unresolved	Kd >20 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 20; SPR Kd (µM) column
SYB10	NSD3 PWWP, domain instance unresolved	Kd =0.11 ± 0.07 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 23; SPR Kd (µM) column
SYB11	NSD3 PWWP, domain instance unresolved	Kd =0.15 ± 0.03 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 24; SPR Kd (µM) column
SYC1	NSD3 PWWP, domain instance unresolved	Kd =0.13 ± 0.04 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 27; SPR Kd (µM) column
SYC3	NSD3 PWWP, domain instance unresolved	Kd =0.32 ± 0.11 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 29; SPR Kd (µM) column
SYC5	NSD3 PWWP, domain instance unresolved	Kd =0.18 ± 0.03 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 31; SPR Kd (µM) column
SYC6	NSD3 PWWP, domain instance unresolved	Kd =0.25 ± 0.06 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 32; SPR Kd (µM) column
SYC7	NSD3 PWWP, domain instance unresolved	Kd =7.69 ± 0.32 µM SPR (publisher compound table header)	10.1021/acs.jmedchem.6c00983 Publisher source compound table row 33; SPR Kd (µM) column

Evidence classification · exact source records

26 matching profiles

72 matching assay / association observations. Counts here are profiles, not unique assay events or deposited ligand instances; views overlap.

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Verified 2D molecular structure of BI-9321

Studied + deposited research ligandReported probe

BI-9321

NSD3

SPR Kd= 166 ± 3 nMOne source observation · PMID:31285596

3 matching observations1 PWWP research-ligand PDB entries

PWWP Compounds& Bioactivity

13 matching new observations · 12 preparations

26 matching profiles

PWWP Compounds
& Bioactivity