Scientific evidence classification · Phase 6A preserved + Phase 6B additions

PWWP Compounds
& Bioactivity

Explore experimentally studied preparations separately from deposited research ligands, buffers, ions and unresolved chemical evidence. Every role is source- and target-context specific.

Explore studied compounds Structural & Binding Landscape →

Different denominators · 26 October 2026

38studied preparation profiles · 26 baseline + 12 new

109profiles with PWWP research-ligand geometry

93source assay / association observations · 80 baseline + 13 new

Combined Phase 6A + Phase 6B denominators. These groups overlap; the 109 structurally source-tagged research ligands are unchanged. Deposited components and reagents are not automatically biochemical binders.

Search compounds & identifiersProteinEvidence view

Specific PWWP domainExact PWWP-proximal deposited siteChemical identity

Assay, source & experimental-context filters

Assay method Endpoint Reported relation Exact concentration min (µM)Exact concentration max (µM) Publication / source databasePublication yearAssay organismTested constructVerification status / scope Missing experimental detailAny associated PDB structure Inventory scopeSort

Assay filters must match the same observation. Concentration ranges require one endpoint and exact positive measurements; inequalities and qualitative findings remain searchable separately.

Phase 6B · source-reviewed additions (separate from the Phase 6A snapshot)

2 matching new observations · 1 preparations

Keep source-level IC50, Kd, and whole-protein DC50 separate; these records were not included in the original 80.

Filter all Phase 6B assay contexts

Preparation	Target scope	Original assay	Primary source
UNC10088	NSD2 PWWP1, exact domain	IC50 =248 ± 22 nM TR-FRET competitive displacement of biotinylated UNC7096	10.1038/s41467-026-72235-9 Figure 2h and Supplementary Figure 3D
UNC10088	NSD2 PWWP1, exact domain	IC50 =101 ± 8 nM TR-FRET competitive displacement of biotinylated UNC7096	10.1038/s41467-026-72235-9 Figure 2h, Supplementary Figure 3D and Results

Evidence classification · exact source records

31 matching profiles

34 matching assay / association observations. Counts here are profiles, not unique assay events or deposited ligand instances; views overlap.

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Preparation	Tested target & construct	Method / endpoint	Original observation	Conditions & uncertainty	Source & verification
UNC6934PREP:UNC6934	NSD2 PWWP1 208–368Homo sapiens	IC50AlphaScreen IC50biochemical interaction inhibition	= 104 ± 13 nM0.104 µM · exact unit conversion	Experimental context Replicates 3 independent experiments; each 10-point curve in duplicate Uncertainty definition Not curated; do not assume SD or SEM Temperature 23 °C Buffer 150 mM NaCl; 20 mM Tris; 0.01% BSA; 0.01% NP-40; 1 mM DTT (DTT omitted from bead buffer) Conditions {"phase3_verification": "SV02A", "protein_construct": "NSD2 208–368", "readout": "His-NSD2-PWWP1 binding to H3K36me2 designer nucleosome", "tag": "6His", "temperature_scope": "23°C compound/protein and nucleosome preincubation; bead incubation room temperature"} Qualification 23°C is the antagonist preincubation temperature, not a verified readout temperature. Final DMSO fraction and SD-versus-SEM definition for ±13 nM are not explicit in the inspected assay-specific text.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0005", "assay_type": "AlphaScreen IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0005\", \"assay_type\": \"AlphaScreen IC50\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Discussion; biochemical characterization\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"Binding-interaction assay, not a direct catalytic SET-domain inhibition measurement.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6XCG\", \"reference_id\": \"PMID:34782742\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 13, \"units\": \"nM\", \"value\": 104, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "150 mM NaCl; 20 mM Tris; 0.01% BSA; 0.01% NP-40; 1 mM DTT (DTT omitted from bead buffer)", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"phase3_verification\": \"SV02A\", \"protein_construct\": \"NSD2 208\u2013368\", \"readout\": \"His-NSD2-PWWP1 binding to H3K36me2 designer nucleosome\", \"tag\": \"6His\", \"temperature_scope\": \"23\u00b0C compound/protein and nucleosome preincubation; bead incubation room temperature\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Discussion; biochemical characterization", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": "Binding-interaction assay, not a direct catalytic SET-domain inhibition measurement.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "6XCG", "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "23\u00b0C is the antagonist preincubation temperature, not a verified readout temperature. Final DMSO fraction and SD-versus-SEM definition for \u00b113 nM are not explicit in the inspected assay-specific text.", "replicates": "3 independent experiments; each 10-point curve in duplicate", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": "13", "source_reported_units": "nM", "source_reported_value": "104", "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": 23, "uncertainty": 13.0, "uncertainty_type": null, "uncertainty_uM": 0.013, "unit_conversion": null, "units": "nM", "value": 104.0, "value_operator": "=", "value_uM": 0.104, "verification_level": "primary_full_text_sections_verified" }
compound 12 (Collie 2025)PREP:COLLIE2025_12	NSD2 PWWP1 208–368Homo sapiens	IC50HTRF displacement IC50biochemical interaction inhibition	= 2.49 ± 0.06 µM2.49 µM · exact unit conversion	Experimental context Replicates 2 Uncertainty definition SEM Temperature — Buffer Not curated Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": 2, "uncertainty_type": "SEM"} Qualification HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts. Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0030", "assay_type": "HTRF displacement IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0030\", \"assay_type\": \"HTRF displacement IC50\", \"ccd_id\": null, \"compound_name\": \"compound 12 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": 2, \"uncertainty_type\": \"SEM\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.06, \"units\": \"\u00b5M\", \"value\": 2.49, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 12 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": 2, \"uncertainty_type\": \"SEM\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_12", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.", "replicates": "2", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.06", "source_reported_units": "\u00b5M", "source_reported_value": "2.49", "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.", "temperature_C": null, "uncertainty": 0.06, "uncertainty_type": "SEM", "uncertainty_uM": 0.06, "unit_conversion": null, "units": "\u00b5M", "value": 2.49, "value_operator": "=", "value_uM": 2.49, "verification_level": "primary_full_text_sections_verified" }
compound 13 (Collie 2025)PREP:COLLIE2025_13	NSD2 PWWP1 208–368Homo sapiens	IC50HTRF displacement IC50biochemical interaction inhibition	= 1.31 ± 0.06 µM1.31 µM · exact unit conversion	Experimental context Replicates at least 3 Uncertainty definition SEM Temperature — Buffer Not curated Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SEM"} Qualification HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts. Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0031", "assay_type": "HTRF displacement IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0031\", \"assay_type\": \"HTRF displacement IC50\", \"ccd_id\": null, \"compound_name\": \"compound 13 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SEM\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.06, \"units\": \"\u00b5M\", \"value\": 1.31, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 13 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SEM\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_13", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.", "replicates": "at least 3", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.06", "source_reported_units": "\u00b5M", "source_reported_value": "1.31", "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.", "temperature_C": null, "uncertainty": 0.06, "uncertainty_type": "SEM", "uncertainty_uM": 0.06, "unit_conversion": null, "units": "\u00b5M", "value": 1.31, "value_operator": "=", "value_uM": 1.31, "verification_level": "primary_full_text_sections_verified" }
compound 6 (Collie 2025)PREP:COLLIE2025_6	NSD2 PWWP1 208–368Homo sapiens	IC50HTRF displacement IC50biochemical interaction inhibition	= 1.24 ± 0.08 µM1.24 µM · exact unit conversion	Experimental context Replicates 2 Uncertainty definition SEM Temperature — Buffer Not curated Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": 2, "uncertainty_type": "SEM"} Qualification HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts. Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0028", "assay_type": "HTRF displacement IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0028\", \"assay_type\": \"HTRF displacement IC50\", \"ccd_id\": null, \"compound_name\": \"compound 6 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": 2, \"uncertainty_type\": \"SEM\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.08, \"units\": \"\u00b5M\", \"value\": 1.24, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 6 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": 2, \"uncertainty_type\": \"SEM\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_6", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.", "replicates": "2", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.08", "source_reported_units": "\u00b5M", "source_reported_value": "1.24", "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.", "temperature_C": null, "uncertainty": 0.08, "uncertainty_type": "SEM", "uncertainty_uM": 0.08, "unit_conversion": null, "units": "\u00b5M", "value": 1.24, "value_operator": "=", "value_uM": 1.24, "verification_level": "primary_full_text_sections_verified" }
compound 7 (Collie 2025)PREP:COLLIE2025_7	NSD2 PWWP1 208–368Homo sapiens	IC50HTRF displacement IC50biochemical interaction inhibition	= 0.21 ± 0.01 µM0.21 µM · exact unit conversion	Experimental context Replicates at least 3 Uncertainty definition SEM Temperature — Buffer Not curated Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SEM"} Qualification HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts. Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0029", "assay_type": "HTRF displacement IC50", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0029\", \"assay_type\": \"HTRF displacement IC50\", \"ccd_id\": \"A1IEF\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"compound 7 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SEM\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOE\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.01, \"units\": \"\u00b5M\", \"value\": 0.21, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "A1IEF", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "compound 7 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SEM\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "biochemical_interaction_inhibition", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "9FOE", "preparation_id": "PREP:COLLIE2025_7", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.", "replicates": "at least 3", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.01", "source_reported_units": "\u00b5M", "source_reported_value": "0.21", "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.", "temperature_C": null, "uncertainty": 0.01, "uncertainty_type": "SEM", "uncertainty_uM": 0.01, "unit_conversion": null, "units": "\u00b5M", "value": 0.21, "value_operator": "=", "value_uM": 0.21, "verification_level": "primary_full_text_sections_verified" }
MR837 (3f)PREP:MR837	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 7 ± 3 µM7 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {} Qualification Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.	PMID:33522809 · 2021primary_article_selected_indexed_full_text_passages; original figures and supplement not independently opened Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0001", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0001\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"Q5D\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"MR837 (3f)\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results; Figure 2\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/\", \"gene\": \"NSD2\", \"interpretation\": \"Distinct condition from the lower-DMSO SPR measurement; do not average.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6UE6\", \"reference_id\": \"PMID:33522809\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 3, \"units\": \"\u00b5M\", \"value\": 7, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "Q5D", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:WYAGGMZTARRIDX-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "MR837 (3f)", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 2", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "interpretation": "Distinct condition from the lower-DMSO SPR measurement; do not average.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "6UE6", "preparation_id": "PREP:MR837", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:33522809", "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.", "replicates": null, "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S01", "source_reported_uncertainty": "3", "source_reported_units": "\u00b5M", "source_reported_value": "7", "source_verification_scope": "primary_article_selected_indexed_full_text_passages; original figures and supplement not independently opened", "temperature_C": null, "uncertainty": 3.0, "uncertainty_type": null, "uncertainty_uM": 3.0, "unit_conversion": null, "units": "\u00b5M", "value": 7.0, "value_operator": "=", "value_uM": 7.0, "verification_level": "primary_full_text_sections_verified" }
MR837 (3f)PREP:MR837	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 3.4 ± 0.4 µM3.4 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {"DMSO_percent": 0.5} Qualification Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.	PMID:33522809 · 2021primary_article_selected_indexed_full_text_passages; original figures and supplement not independently opened Original observation record { "DMSO_percent": 0.5, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0002", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0002\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"Q5D\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"MR837 (3f)\", \"conditions\": {\"DMSO_percent\": 0.5}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results; Figure S6\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/\", \"gene\": \"NSD2\", \"interpretation\": \"Lower-DMSO measurement; preserve assay conditions.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6UE6\", \"reference_id\": \"PMID:33522809\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.4, \"units\": \"\u00b5M\", \"value\": 3.4, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "Q5D", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:WYAGGMZTARRIDX-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "MR837 (3f)", "conditions": "{\"DMSO_percent\": 0.5}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure S6", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "interpretation": "Lower-DMSO measurement; preserve assay conditions.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "6UE6", "preparation_id": "PREP:MR837", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:33522809", "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.", "replicates": null, "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S01", "source_reported_uncertainty": "0.4", "source_reported_units": "\u00b5M", "source_reported_value": "3.4", "source_verification_scope": "primary_article_selected_indexed_full_text_passages; original figures and supplement not independently opened", "temperature_C": null, "uncertainty": 0.4, "uncertainty_type": null, "uncertainty_uM": 0.4, "unit_conversion": null, "units": "\u00b5M", "value": 3.4, "value_operator": "=", "value_uM": 3.4, "verification_level": "primary_full_text_sections_verified" }
MRT866PREP:MRT866	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 349 ± 19 nM0.349 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {} Qualification 349 ± 19 nM verified in Results; MRT866-specific SPR construct/protocol, replicate definition and uncertainty statistic remain unresolved. Crystallography 211–350 is not assigned as the binding-assay construct.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0003", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0003\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"Y6V\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"MRT866\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results: Discovery of a potent ligand targeting NSD2-PWWP1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"7MDN\", \"reference_id\": \"PMID:34782742\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 19, \"units\": \"nM\", \"value\": 349, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "Y6V", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:XPHOPAPMJBSLFN-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "MRT866", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results: Discovery of a potent ligand targeting NSD2-PWWP1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": null, "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "7MDN", "preparation_id": "PREP:MRT866", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "349 \u00b1 19 nM verified in Results; MRT866-specific SPR construct/protocol, replicate definition and uncertainty statistic remain unresolved. Crystallography 211\u2013350 is not assigned as the binding-assay construct.", "replicates": null, "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": "19", "source_reported_units": "nM", "source_reported_value": "349", "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": null, "uncertainty": 19.0, "uncertainty_type": null, "uncertainty_uM": 0.019, "unit_conversion": null, "units": "nM", "value": 349.0, "value_operator": "=", "value_uM": 0.349, "verification_level": "primary_full_text_sections_verified" }
UNC6934PREP:UNC6934	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 91 ± 8 nM0.091 µM · exact unit conversion	Experimental context Replicates 3 (triplicate experiments; independence level not specified) Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO Conditions {"phase3_verification": "SV02A", "protein_construct": "NSD2 208–368", "purified_tags": "AviTag/NSD2(208–368)/polyhistidine; biotinylated", "source_protocol_highest_concentration_uM": 2} Qualification SPR temperature and exact uncertainty definition are not specified in the inspected assay-specific source. Triplicate experiments reported. Preparation/assay construct differs from crystallization 211–350.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": 0.5, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0004", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0004\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results; Figures 1\u20132\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6XCG\", \"reference_id\": \"PMID:34782742\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 8, \"units\": \"nM\", \"value\": 91, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"phase3_verification\": \"SV02A\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"AviTag/NSD2(208\u2013368)/polyhistidine; biotinylated\", \"source_protocol_highest_concentration_uM\": 2}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figures 1\u20132", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": null, "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.4, "pdb_id": "6XCG", "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "SPR temperature and exact uncertainty definition are not specified in the inspected assay-specific source. Triplicate experiments reported. Preparation/assay construct differs from crystallization 211\u2013350.", "replicates": "3 (triplicate experiments; independence level not specified)", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": "8", "source_reported_units": "nM", "source_reported_value": "91", "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": null, "uncertainty": 8.0, "uncertainty_type": null, "uncertainty_uM": 0.008, "unit_conversion": null, "units": "nM", "value": 91.0, "value_operator": "=", "value_uM": 0.091, "verification_level": "primary_full_text_sections_verified" }
UNC6934PREP:UNC6934	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.24 ± 0.03 µM0.24 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0014", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0014\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 0.03, \"units\": \"\u00b5M\", \"value\": 0.24, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.03", "source_reported_units": "\u00b5M", "source_reported_value": "0.24", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.03, "uncertainty_type": "SD", "uncertainty_uM": 0.03, "unit_conversion": null, "units": "\u00b5M", "value": 0.24, "value_operator": "=", "value_uM": 0.24, "verification_level": "primary_full_text_sections_verified" }
UNC6934PREP:UNC6934	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 1.33 ± 0.19 µM1.33 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0021", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0021\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 0.19, \"units\": \"\u00b5M\", \"value\": 1.33, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.19", "source_reported_units": "\u00b5M", "source_reported_value": "1.33", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.19, "uncertainty_type": "SD", "uncertainty_uM": 0.19, "unit_conversion": null, "units": "\u00b5M", "value": 1.33, "value_operator": "=", "value_uM": 1.33, "verification_level": "primary_full_text_sections_verified" }
UNC7096PREP:UNC7096	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 46 nM0.046 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification UNC7096-specific construct, buffer, uncertainty and replicate definition were not independently verified; the general UNC6934/UNC7145 SPR protocol is not automatically transferred.	PMID:34782742 · 2022NCBI BioC primary full text; UNC7096 Results and UNC7145 chemical transformation; primary Methods/captions inspected Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0007", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:JXTDBIVBSAFNBJ-PLCURSASSA-N", "compound_name": "UNC7096", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results: Development of selective affinity reagents; Extended Data Figure 6", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC7096", "protein_id": "UP:O96028", "publication_year": 2022, "reference_id": "PMID:34782742", "remaining_uncertainty": "UNC7096-specific construct, buffer, uncertainty and replicate definition were not independently verified; the general UNC6934/UNC7145 SPR protocol is not automatically transferred.", "replicates": null, "retrieval_date": "2026-10-26", "role": "affinity_reagent", "source_id": "P5:S:DILWORTH2021", "source_reported_uncertainty": null, "source_reported_units": "nM", "source_reported_value": "46", "source_verification_scope": "NCBI BioC primary full text; UNC7096 Results and UNC7145 chemical transformation; primary Methods/captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "nM", "value": 46, "value_operator": "=", "value_uM": 0.046, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8153PREP:UNC8153	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 24 ± 7 nM0.024 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification Original value and uncertainty preserved from later primary-table reprint; original Figure 3 numeric image not separately opened. Original experiment replicate/statistic assignment and construct remain uncurated; this is not a second 2024 experiment.	PMID:36976643 · 2023NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1 Original observation record { "additional_source_urls": [ "https://www.nature.com/articles/s41589-024-01660-y/tables/1" ], "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0009", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:MDIKJIFCQAJLPD-UHFFFAOYSA-N", "compound_name": "UNC8153", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Figure 3A; numeric value visually verified in asterisk-marked original-data row of Nie 2024 Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC10116495/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8153", "protein_id": "UP:O96028", "publication_year": 2023, "reference_id": "PMID:36976643", "remaining_uncertainty": "Original value and uncertainty preserved from later primary-table reprint; original Figure 3 numeric image not separately opened. Original experiment replicate/statistic assignment and construct remain uncurated; this is not a second 2024 experiment.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:HANLEY2023", "source_reported_uncertainty": "7", "source_reported_units": "nM", "source_reported_value": "24", "source_verification_scope": "NCBI BioC primary full text and captions; exact UNC8153 values additionally checked in reused original-data row of Nie 2024 Table 1", "uncertainty": 7, "uncertainty_type": null, "uncertainty_uM": 0.007, "underlying_measurement_id": "HANLEY2023:UNC8153:SPR", "units": "nM", "value": 24, "value_operator": "=", "value_uM": 0.024, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8269PREP:UNC8269	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 36 ± 21 nM0.036 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions Not curated Qualification Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0015", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8269", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1; Supplementary Figure 1", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8269", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "21", "source_reported_units": "nM", "source_reported_value": "36", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 21, "uncertainty_type": "SD", "uncertainty_uM": 0.021, "units": "nM", "value": 36, "value_operator": "=", "value_uM": 0.036, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8325PREP:UNC8325	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 41 ± 19 nM0.041 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions Not curated Qualification Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0018", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8325", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1; Supplementary Figure 1", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8325", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "19", "source_reported_units": "nM", "source_reported_value": "41", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 19, "uncertainty_type": "SD", "uncertainty_uM": 0.019, "units": "nM", "value": 41, "value_operator": "=", "value_uM": 0.041, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
UNC8732PREP:UNC8732	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 76 ± 4 nM0.076 µM · exact unit conversion	Experimental context Replicates 3 independent experiments Uncertainty definition SD Temperature °C Buffer Not curated Conditions Not curated Qualification Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0021", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:CSTHTJLTORVUST-UHFFFAOYSA-N", "compound_name": "UNC8732", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1; Supplementary Figure 1", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8732", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38965384", "remaining_uncertainty": "Exact domain is stated; assay construct boundaries and full buffer were not independently recovered. Original table image and supporting SPR panel inspected.", "replicates": "3 independent experiments", "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": "4", "source_reported_units": "nM", "source_reported_value": "76", "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": 4, "uncertainty_type": "SD", "uncertainty_uM": 0.004, "units": "nM", "value": 76, "value_operator": "=", "value_uM": 0.076, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
compound 1 (de Freitas 2021)PREP:DEFREITAS2021_1	NSD2 PWWP1 Construct not curatedHomo sapiens	KdSPR Kddirect binding affinity	= 41 ± 8 µM41 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification Source text directly reports preparation and Kd ± uncertainty; supplementary figure and compound-1-specific buffer/construct/statistic not independently recovered. The SPR Methods paragraph explicitly names 3f, so its conditions are not copied to compound 1.	PMID:33522809 · 2021NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0033", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "compound 1 (de Freitas 2021)", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure S1a", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:DEFREITAS2021_1", "protein_id": "UP:O96028", "publication_year": 2021, "reference_id": "PMID:33522809", "remaining_uncertainty": "Source text directly reports preparation and Kd \u00b1 uncertainty; supplementary figure and compound-1-specific buffer/construct/statistic not independently recovered. The SPR Methods paragraph explicitly names 3f, so its conditions are not copied to compound 1.", "replicates": null, "retrieval_date": "2026-10-26", "role": "fragment_hit", "source_id": "P5:S:DEFREITAS2021", "source_reported_uncertainty": "8", "source_reported_units": "\u00b5M", "source_reported_value": "41", "source_verification_scope": "NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved", "uncertainty": 8, "uncertainty_type": null, "uncertainty_uM": 8, "units": "\u00b5M", "value": 41, "value_operator": "=", "value_uM": 41, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
compound 1 (de Freitas 2021)PREP:DEFREITAS2021_1	NSD2 PWWP1 Construct not curatedHomo sapiens	KdReverse ITC Kddirect binding affinity	= 8.9 µM8.9 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions {'compound_cell_concentration_uM': 40, 'protein_syringe_concentration_mM': 1, 'reverse_titration_reason': 'Limited compound solubility', 'titration_direction': 'Protein into compound'} Qualification No uncertainty, replicate count or exact stereochemical assignment inferred from the primary Results value. A distinct reverse-ITC experiment is retained separately from SPR.	PMID:33522809 · 2021NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0034", "assay_type": "Reverse ITC Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "compound 1 (de Freitas 2021)", "conditions": { "compound_cell_concentration_uM": 40, "protein_syringe_concentration_mM": 1, "reverse_titration_reason": "Limited compound solubility", "titration_direction": "Protein into compound" }, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure S1b", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8130708/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:DEFREITAS2021_1", "protein_id": "UP:O96028", "publication_year": 2021, "reference_id": "PMID:33522809", "remaining_uncertainty": "No uncertainty, replicate count or exact stereochemical assignment inferred from the primary Results value. A distinct reverse-ITC experiment is retained separately from SPR.", "replicates": null, "retrieval_date": "2026-10-26", "role": "fragment_hit", "source_id": "P5:S:DEFREITAS2021", "source_reported_uncertainty": null, "source_reported_units": "\u00b5M", "source_reported_value": "8.9", "source_verification_scope": "NCBI BioC primary full text; Results, SPR Methods and figure captions; supplementary numerical figure images not independently retrieved", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "\u00b5M", "value": 8.9, "value_operator": "=", "value_uM": 8.9, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
compound 11 (Collie 2025)PREP:COLLIE2025_11	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 1.02 ± 0.19 µM1.02 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0018", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0018\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 11 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"crystallographic_component_ids\": [\"A1IFS\", \"A1IEG\"], \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded. Compound 11 is the assayed preparation; 9FOC models two stereoisomer CCDs. Do not assign the aggregate affinity separately to each pure stereoisomer.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOC\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.19, \"units\": \"\u00b5M\", \"value\": 1.02, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 11 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": "[\"A1IFS\", \"A1IEG\"]", "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded. Compound 11 is the assayed preparation; 9FOC models two stereoisomer CCDs. Do not assign the aggregate affinity separately to each pure stereoisomer.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "9FOC", "preparation_id": "PREP:COLLIE2025_11", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.19", "source_reported_units": "\u00b5M", "source_reported_value": "1.02", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.19, "uncertainty_type": "SD", "uncertainty_uM": 0.19, "unit_conversion": null, "units": "\u00b5M", "value": 1.02, "value_operator": "=", "value_uM": 1.02, "verification_level": "primary_full_text_sections_verified" }
compound 11 (Collie 2025)PREP:COLLIE2025_11	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 2.3 ± 0.31 µM2.3 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0025", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0025\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 11 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"crystallographic_component_ids\": [\"A1IFS\", \"A1IEG\"], \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded. Compound 11 is the assayed preparation; 9FOC models two stereoisomer CCDs. Do not assign the aggregate affinity separately to each pure stereoisomer.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOC\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.31, \"units\": \"\u00b5M\", \"value\": 2.3, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 11 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": "[\"A1IFS\", \"A1IEG\"]", "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded. Compound 11 is the assayed preparation; 9FOC models two stereoisomer CCDs. Do not assign the aggregate affinity separately to each pure stereoisomer.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "9FOC", "preparation_id": "PREP:COLLIE2025_11", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.31", "source_reported_units": "\u00b5M", "source_reported_value": "2.3", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.31, "uncertainty_type": "SD", "uncertainty_uM": 0.31, "unit_conversion": null, "units": "\u00b5M", "value": 2.3, "value_operator": "=", "value_uM": 2.3, "verification_level": "primary_full_text_sections_verified" }
compound 12 (Collie 2025)PREP:COLLIE2025_12	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 1.47 ± 0.13 µM1.47 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0019", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0019\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 12 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.13, \"units\": \"\u00b5M\", \"value\": 1.47, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 12 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_12", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.13", "source_reported_units": "\u00b5M", "source_reported_value": "1.47", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.13, "uncertainty_type": "SD", "uncertainty_uM": 0.13, "unit_conversion": null, "units": "\u00b5M", "value": 1.47, "value_operator": "=", "value_uM": 1.47, "verification_level": "primary_full_text_sections_verified" }
compound 12 (Collie 2025)PREP:COLLIE2025_12	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 2.8 ± 0.31 µM2.8 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0026", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0026\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 12 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.31, \"units\": \"\u00b5M\", \"value\": 2.8, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 12 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_12", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.31", "source_reported_units": "\u00b5M", "source_reported_value": "2.8", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.31, "uncertainty_type": "SD", "uncertainty_uM": 0.31, "unit_conversion": null, "units": "\u00b5M", "value": 2.8, "value_operator": "=", "value_uM": 2.8, "verification_level": "primary_full_text_sections_verified" }
compound 13 (Collie 2025)PREP:COLLIE2025_13	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.75 ± 0.04 µM0.75 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0020", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0020\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 13 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.04, \"units\": \"\u00b5M\", \"value\": 0.75, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 13 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_13", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.04", "source_reported_units": "\u00b5M", "source_reported_value": "0.75", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.04, "uncertainty_type": "SD", "uncertainty_uM": 0.04, "unit_conversion": null, "units": "\u00b5M", "value": 0.75, "value_operator": "=", "value_uM": 0.75, "verification_level": "primary_full_text_sections_verified" }
compound 13 (Collie 2025)PREP:COLLIE2025_13	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 1.52 ± 0.05 µM1.52 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0027", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0027\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 13 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.05, \"units\": \"\u00b5M\", \"value\": 1.52, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 13 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_13", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.05", "source_reported_units": "\u00b5M", "source_reported_value": "1.52", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.05, "uncertainty_type": "SD", "uncertainty_uM": 0.05, "unit_conversion": null, "units": "\u00b5M", "value": 1.52, "value_operator": "=", "value_uM": 1.52, "verification_level": "primary_full_text_sections_verified" }
compound 5 (Collie 2025)PREP:COLLIE2025_5	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.16 ± 0.03 µM0.16 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0015", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0015\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 5 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.03, \"units\": \"\u00b5M\", \"value\": 0.16, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 5 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_5", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.03", "source_reported_units": "\u00b5M", "source_reported_value": "0.16", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.03, "uncertainty_type": "SD", "uncertainty_uM": 0.03, "unit_conversion": null, "units": "\u00b5M", "value": 0.16, "value_operator": "=", "value_uM": 0.16, "verification_level": "primary_full_text_sections_verified" }
compound 5 (Collie 2025)PREP:COLLIE2025_5	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 0.92 ± 0.03 µM0.92 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0022", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0022\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 5 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.03, \"units\": \"\u00b5M\", \"value\": 0.92, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity", "component_context": null, "compound_name": "compound 5 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_5", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.03", "source_reported_units": "\u00b5M", "source_reported_value": "0.92", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.03, "uncertainty_type": "SD", "uncertainty_uM": 0.03, "unit_conversion": null, "units": "\u00b5M", "value": 0.92, "value_operator": "=", "value_uM": 0.92, "verification_level": "primary_full_text_sections_verified" }
compound 6 (Collie 2025)PREP:COLLIE2025_6	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.71 ± 0.09 µM0.71 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0016", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0016\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 6 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.09, \"units\": \"\u00b5M\", \"value\": 0.71, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 6 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_6", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.09", "source_reported_units": "\u00b5M", "source_reported_value": "0.71", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.09, "uncertainty_type": "SD", "uncertainty_uM": 0.09, "unit_conversion": null, "units": "\u00b5M", "value": 0.71, "value_operator": "=", "value_uM": 0.71, "verification_level": "primary_full_text_sections_verified" }
compound 6 (Collie 2025)PREP:COLLIE2025_6	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 5.83 ± 0.64 µM5.83 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0023", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0023\", \"assay_type\": \"SPR Kd\", \"ccd_id\": null, \"compound_name\": \"compound 6 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.64, \"units\": \"\u00b5M\", \"value\": 5.83, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "compound 6 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": null, "preparation_id": "PREP:COLLIE2025_6", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.64", "source_reported_units": "\u00b5M", "source_reported_value": "5.83", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.64, "uncertainty_type": "SD", "uncertainty_uM": 0.64, "unit_conversion": null, "units": "\u00b5M", "value": 5.83, "value_operator": "=", "value_uM": 5.83, "verification_level": "primary_full_text_sections_verified" }
compound 7 (Collie 2025)PREP:COLLIE2025_7	NSD2 PWWP1 208–368Homo sapiens	KdSPR Kddirect binding affinity	= 0.15 ± 0.02 µM0.15 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 208–368", "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0017", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0017\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"A1IEF\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"compound 7 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 208\u2013368\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOE\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.02, \"units\": \"\u00b5M\", \"value\": 0.15, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "A1IEF", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "compound 7 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "9FOE", "preparation_id": "PREP:COLLIE2025_7", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.02", "source_reported_units": "\u00b5M", "source_reported_value": "0.15", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.02, "uncertainty_type": "SD", "uncertainty_uM": 0.02, "unit_conversion": null, "units": "\u00b5M", "value": 0.15, "value_operator": "=", "value_uM": 0.15, "verification_level": "primary_full_text_sections_verified" }
compound 7 (Collie 2025)PREP:COLLIE2025_7	NSD2 PWWP1 211–350Homo sapiens	KdSPR Kddirect binding affinity	= 0.76 ± 0.01 µM0.76 µM · exact unit conversion	Experimental context Replicates at least 3 independent replicates (Table 1 general SPR definition) Uncertainty definition SD Temperature 25 °C Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO Conditions {"assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate", "phase3_verification": "SV05A/SV05B", "protein_construct": "NSD2 211–350", "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal", "replicates": "at least 3", "uncertainty_type": "SD"} Qualification Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.	PMID:40959233 · 2025Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected Original observation record { "DMSO_percent": 0.3, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 350, "assay_construct_start": 211, "assay_record_id": "PWWP:ASSAY:0024", "assay_type": "SPR Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0024\", \"assay_type\": \"SPR Kd\", \"ccd_id\": \"A1IEF\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"compound 7 (Collie 2025)\", \"conditions\": {\"protein_construct\": \"NSD2 211\u2013350\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Table 1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/\", \"gene\": \"NSD2\", \"interpretation\": \"Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"9FOE\", \"reference_id\": \"PMID:40959233\", \"role\": \"experimentally_studied_ligand\", \"uncertainty\": 0.01, \"units\": \"\u00b5M\", \"value\": 0.76, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO", "ccd_id": "A1IEF", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "compound 7 (Collie 2025)", "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 211\u2013350\", \"purified_tags\": \"N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal\", \"replicates\": \"at least 3\", \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/", "gene": "NSD2", "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.5, "pdb_id": "9FOE", "preparation_id": "PREP:COLLIE2025_7", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:40959233", "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.", "replicates": "at least 3 independent replicates (Table 1 general SPR definition)", "role": "experimentally_studied_ligand", "source_component_url": null, "source_id": "S05", "source_reported_uncertainty": "0.01", "source_reported_units": "\u00b5M", "source_reported_value": "0.76", "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected", "temperature_C": 25, "uncertainty": 0.01, "uncertainty_type": "SD", "uncertainty_uM": 0.01, "unit_conversion": null, "units": "\u00b5M", "value": 0.76, "value_operator": "=", "value_uM": 0.76, "verification_level": "primary_full_text_sections_verified" }
HS13PREP:HS13	NSD2 PWWP1 Construct not curatedHomo sapiens	Qualitative / associationQuantitative assay verification pendingabstract reported covalent association	No quantitative observation curated	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {} Qualification Primary abstract reports Kd 8.33 µM and C294 covalency; full assay method, construct, uncertainty and exact chemical identity not verified. Main value remains null.	PMID:42442058 · 2026PubMed_primary_article_abstract_only Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": "Kd", "abstract_reported_method": null, "abstract_reported_units": "\u00b5M", "abstract_reported_value": 8.33, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0035", "assay_type": "Quantitative assay verification pending", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0035\", \"assay_type\": \"Quantitative assay verification pending\", \"ccd_id\": null, \"compound_name\": \"HS13\", \"conditions\": {}, \"domain\": \"PWWP1\", \"evidence_location\": \"Abstract\", \"evidence_url\": \"https://doi.org/10.1016/j.bmc.2026.118751\", \"gene\": \"NSD2\", \"interpretation\": \"Abstract describes C294 covalency and an affinity value; full assay details were inaccessible, so numerical pharmacology is intentionally uncurated.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:42442058\", \"role\": \"reported_covalent_ligand\", \"uncertainty\": null, \"units\": null, \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"abstract_only_quantitative_verification_pending\"}", "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "HS13", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "abstract_reported_covalent_association", "evidence_location": "Abstract", "evidence_url": "https://doi.org/10.1016/j.bmc.2026.118751", "gene": "NSD2", "interpretation": "Abstract describes C294 covalency and an affinity value; full assay details were inaccessible, so numerical pharmacology is intentionally uncurated.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "not_a_quantitative_affinity_record", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": null, "preparation_id": "PREP:HS13", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:42442058", "remaining_uncertainty": "Primary abstract reports Kd 8.33 \u00b5M and C294 covalency; full assay method, construct, uncertainty and exact chemical identity not verified. Main value remains null.", "replicates": null, "role": "reported_covalent_ligand", "source_component_url": null, "source_id": "S08", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "PubMed_primary_article_abstract_only", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "abstract_only_quantitative_verification_pending" }
UNC7145PREP:UNC7145Negative / censored	NSD2 PWWP1 208–368Homo sapiens	Qualitative / associationSPR no appreciable bindingnegative binding test	No quantitative observation curated	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO Conditions {"highest_tested_concentration_uM": 20, "phase3_verification": "SV02A", "purified_tags": "AviTag/NSD2(208–368)/polyhistidine; biotinylated", "source_protocol_highest_concentration_uM": 2} Qualification Primary-source concentration discrepancy: Results report no appreciable binding up to 20 uM, but SPR Methods state 2 uM as highest concentration. Neither is converted into a Kd bound; human source review required.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": 0.5, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 368, "assay_construct_start": 208, "assay_record_id": "PWWP:ASSAY:0007", "assay_type": "SPR no appreciable binding", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0007\", \"assay_type\": \"SPR no appreciable binding\", \"ccd_id\": null, \"compound_name\": \"UNC7145\", \"conditions\": {\"highest_tested_concentration_uM\": 20}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results: Discovery of a potent ligand targeting NSD2-PWWP1\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"No appreciable binding up to 20 \u00b5M; this is not a measured infinite Kd.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:34782742\", \"role\": \"negative_control\", \"uncertainty\": null, \"units\": \"\u00b5M\", \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "paper_label_exact_identity_unresolved", "component_context": null, "compound_name": "UNC7145", "conditions": "{\"highest_tested_concentration_uM\": 20, \"phase3_verification\": \"SV02A\", \"purified_tags\": \"AviTag/NSD2(208\u2013368)/polyhistidine; biotinylated\", \"source_protocol_highest_concentration_uM\": 2}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": null, "evidence_class": "negative_binding_test", "evidence_location": "Results: Discovery of a potent ligand targeting NSD2-PWWP1", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": "No appreciable binding up to 20 \u00b5M; this is not a measured infinite Kd.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "not_a_quantitative_affinity_record", "numeric_value_inferred_from_structure": 0, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:UNC7145", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "Primary-source concentration discrepancy: Results report no appreciable binding up to 20 uM, but SPR Methods state 2 uM as highest concentration. Neither is converted into a Kd bound; human source review required.", "replicates": null, "role": "negative_control", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": null, "source_reported_units": "\u00b5M", "source_reported_value": null, "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": "\u00b5M", "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_full_text_sections_verified" }
UNC6934PREP:UNC6934	NSD2 PWWP1 Construct not curatedHomo sapiens	Unstandardized half-response concentrationNanoBRET PPI half-responsecellular interaction response	= 1.23 ± 0.25 µM	Experimental context Replicates 3 independent replicates Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer DMEM/F12 without phenol red; 4% FBS; 100 U/mL penicillin; 100 ug/mL streptomycin Conditions {"cell_line": "U2OS", "readout": "NSD2 PWWP1–histone H3.3 interaction"} Qualification NanoLuc construct boundaries, readout temperature and SD-versus-SEM are not explicit in the inspected source. Results say EC50 while Discussion says IC50; neutral half-response label retained.	PMID:34782742 · 2022Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0006", "assay_type": "NanoBRET PPI half-response", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0006\", \"assay_type\": \"NanoBRET PPI half-response\", \"ccd_id\": \"V01\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"UNC6934\", \"conditions\": {\"cell_line\": \"U2OS\", \"readout\": \"NSD2 PWWP1\u2013histone H3.3 interaction\"}, \"domain\": \"PWWP1\", \"evidence_location\": \"Results: cellular target engagement; Figure 4d\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/\", \"gene\": \"NSD2\", \"interpretation\": \"Main Results calls this EC50; Discussion calls it IC50. Original value retained without conflating it with Kd.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"6XCG\", \"reference_id\": \"PMID:34782742\", \"role\": \"reported_chemical_probe\", \"uncertainty\": 0.25, \"units\": \"\u00b5M\", \"value\": 1.23, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "DMEM/F12 without phenol red; 4% FBS; 100 U/mL penicillin; 100 ug/mL streptomycin", "ccd_id": "V01", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:KOZGEDUWAQFVAV-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "UNC6934", "conditions": "{\"cell_line\": \"U2OS\", \"readout\": \"NSD2 PWWP1\u2013histone H3.3 interaction\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint_label_status": "Results/Figure use EC50, Discussion uses IC50; neutral half-response retained", "evidence_class": "cellular_interaction_response", "evidence_location": "Results: cellular target engagement; Figure 4d", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/", "gene": "NSD2", "interpretation": "Main Results calls this EC50; Discussion calls it IC50. Original value retained without conflating it with Kd.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "6XCG", "preparation_id": "PREP:UNC6934", "protein_id": "UP:O96028", "qualitative_result": null, "reference_id": "PMID:34782742", "remaining_uncertainty": "NanoLuc construct boundaries, readout temperature and SD-versus-SEM are not explicit in the inspected source. Results say EC50 while Discussion says IC50; neutral half-response label retained.", "replicates": "3 independent replicates", "role": "reported_chemical_probe", "source_component_url": null, "source_id": "S02", "source_reported_uncertainty": "0.25", "source_reported_units": "\u00b5M", "source_reported_value": "1.23", "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained", "temperature_C": null, "uncertainty": 0.25, "uncertainty_type": null, "uncertainty_uM": 0.25, "unit_conversion": null, "units": "\u00b5M", "value": 1.23, "value_operator": "=", "value_uM": 1.23, "verification_level": "primary_full_text_sections_verified" }
UNC8884PREP:UNC8884Negative / censored	NSD2 PWWP1 Construct not curatedHomo sapiens	qualitativeSPR qualitative no bindingqualitative control	NB (no binding); no numeric Kd assigned.	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature °C Buffer Not curated Conditions Not curated Qualification Table reports NB for binding and ND for degradation endpoints; ND means not determined and is not converted to zero or inactivity.	PMID:38965384 · 2024Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures Original observation record { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "purified_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0024", "assay_type": "SPR qualitative no binding", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": null, "compound_name": "UNC8884", "conditions": {}, "domain": "PWWP1", "domain_id": "UP:O96028:PWWP1", "endpoint": "qualitative", "evidence_class": "qualitative_control", "evidence_location": "Table 1; Supplementary Figure 1", "evidence_url": "https://www.nature.com/articles/s41589-024-01660-y", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": true, "negative_control_scope": "Matched NSD2 ligand-binding negative control", "negative_evidence_scope": "Explicit SPR NB result; no numeric threshold or affinity inferred.", "numeric_transcription_status": "qualitative_or_not_reported", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:UNC8884", "protein_id": "UP:O96028", "publication_year": 2024, "qualitative_result": "NB (no binding); no numeric Kd assigned.", "reference_id": "PMID:38965384", "remaining_uncertainty": "Table reports NB for binding and ND for degradation endpoints; ND means not determined and is not converted to zero or inactivity.", "replicates": null, "retrieval_date": "2026-10-26", "role": "negative_control", "source_id": "P5:S:NIE2024", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "Official publisher Table 1 image visually inspected; table footnote and Supplementary Figures 1-2 inspected; identities compared to primary structures", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }

PWWP Compounds& Bioactivity

2 matching new observations · 1 preparations

31 matching profiles

PWWP Compounds
& Bioactivity