Versioned source-reviewed overlay · Phase 6B

Experimental Bioactivity

Primary-source observations, original endpoints, explicit target/construct scope and documented gaps. Phase 5 and Phase 6A remain frozen.

All compound classifications

13additional observed outcomes

12new source preparations

2NSD2 PWWP1 competition observations

150independently triaged source candidates (not validated binders)

Compound / identifierProteinExact domain

Assay, construct, verification, source and structural status

EndpointMethodEvidence type Observed outcomeTarget scope Structure relationship Experimental constructVariantPrimary source / DOI Exact concentration minimum (µM)Exact concentration maximum (µM)Source verification status

All filter predicates must match one observation. Only an exact quantitative endpoint may be filtered by concentration. Units are normalized for filtering without altering the source result.

4 matching observations · 3 preparations

Phase 6B additions only; Phase 6A's baseline 80 remain separately accessible.

Preparation	Target and scope	Method and original endpoint	Source and verification	Structure / limitations
UNC10088published name only exact assayed identity unresolved	NSD2 PWWP1 — exact domainHis-tagged NSD2 PWWP1; exact sequence boundaries not independently extracted	TR-FRET competitive displacement of biotinylated UNC7096 IC50 =248 ± 22 nMSEM · n=3 independent experiments	10.1038/s41467-026-72235-9Figure 2h and Supplementary Figure 3Daccepted with bounded interpretation	Transformed compound in ternary complex — not unmodified parent binary Interpretation and limitations positive competitive engagement; not an equilibrium Kd The prepared chemical parent is not stereochemistry/batch resolved; IC50 not Kd. Do not count FBXO22-conditional results as independent chemical identities. Without FBXO22 FIST domain
UNC10088published name only exact assayed identity unresolved	NSD2 PWWP1 — exact domainHis-tagged NSD2 PWWP1 + FBXO22 FIST; exact sequence boundaries not independently extracted	TR-FRET competitive displacement of biotinylated UNC7096 IC50 =101 ± 8 nMSEM · n=3 independent experiments	10.1038/s41467-026-72235-9Figure 2h, Supplementary Figure 3D and Resultsaccepted with bounded interpretation	Transformed compound in ternary complex — not unmodified parent binary Interpretation and limitations positive cooperative engagement in a ternary context; not an equilibrium Kd Two different experimental contexts for one preparation; do not average or replace one with another. With wild-type FBXO22 FIST domain; source-reported cooperative factor alpha = 3.3, not 248/101
UNC10415667published name only exact assayed identity unresolved	NSD2 — cellular, whole proteinU2OS NSD2-HiBiT cells	Cellular HiBiT protein-degradation dose response DC50 =460 nMfigure caption reports SEM but numeric uncertainty was not independently extracted · n=2 independent experiments (final 2026 article)	10.1038/s41467-026-72235-9Figure 3e legend and Resultsaccepted with bounded interpretation	Transformed compound in ternary complex — not unmodified parent binary Interpretation and limitations cellular whole-protein depletion; no isolated-domain affinity Do not copy this whole-protein DC50 to NSD2 PWWP1. 75% at 1 µM for 6 hours is a separate endpoint, not the same dose-response value. U2OS NSD2 HiBiT dose response
UNC12149published name only exact assayed identity unresolved	NSD2 — cellular, whole proteinU2OS NSD2-HiBiT cells	Cellular HiBiT protein-degradation dose response DC50 =120 nMfigure caption reports SEM but numeric uncertainty was not independently extracted · n=2 independent experiments (final 2026 article)	10.1038/s41467-026-72235-9Figure 4h legend and Resultsaccepted with bounded interpretation	No verified PWWP-bound experimental binary structure Interpretation and limitations cellular whole-protein depletion; no isolated-domain affinity Do not transfer cellular DC50 to a particular PWWP-domain biochemical binding constant. U2OS NSD2 HiBiT dose response

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