Evidence explorer

Structure and assay evidence

Follow HDGFL2 from a deposited contact to its preparation, assay and primary source.

Complete query JSON

9exact contact–assay rows

3assay rows without an exact contact link

4preparation records

HDGFL2 · Structure-linked observations

Exact identity links to deposited contacts

9 rows

9 rows join a geometric contact to an assay on the same native target/domain. Multiple rows may describe the same ligand, structure or measurement. Chain-contact rank is geometric and is not a biological-site assignment.

PDB and chain	Domain / component	Contact evidence	Preparation and measurement	Assay construct and source
8Z6C Chain A	PWWP1 · A1D73 INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N	native annotation core contact Core 2.49 Å Broader 2.49 Å Distance rank 1; multiple chains contact this component	BPP NMR chemical-shift perturbation Kd = 7.0 µM Preparation identity	1–93 Primary evidence Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted. Link interpretation same exact chemical identity and native domain; construct equivalence not assumed
8Z6C Chain C	PWWP1 · A1D73 INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N	native annotation core contact Core 4.81 Å Broader 2.98 Å Distance rank 2; multiple chains contact this component	BPP NMR chemical-shift perturbation Kd = 7.0 µM Preparation identity	1–93 Primary evidence Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted. Link interpretation same exact chemical identity and native domain; construct equivalence not assumed
8Z6C Chain B	PWWP1 · A1D73 INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N	native annotation core contact Core 2.49 Å Broader 2.49 Å Distance rank 1	BPP NMR chemical-shift perturbation Kd = 7.0 µM Preparation identity	1–93 Primary evidence Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted. Link interpretation same exact chemical identity and native domain; construct equivalence not assumed
8Z6C Chain C	PWWP1 · A1D73 INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N	native annotation core contact Core 2.56 Å Broader 2.56 Å Distance rank 1	BPP NMR chemical-shift perturbation Kd = 7.0 µM Preparation identity	1–93 Primary evidence Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted. Link interpretation same exact chemical identity and native domain; construct equivalence not assumed
8Z6O Chain B	PWWP1 · QMR INCHIKEY:JQSHBVHOMNKWFT-DTORHVGOSA-N	native annotation core contact Core 2.81 Å Broader 2.81 Å Distance rank 1	varenicline NMR chemical-shift perturbation Kd = 250.0 µM Preparation identity	1–93 Primary evidence Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent. Link interpretation same exact chemical identity and native domain; construct equivalence not assumed
9G94 Chain A	PWWP1 · A1D73 INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N	native annotation core contact Core 2.26 Å Broader 2.26 Å Distance rank 1	BPP NMR chemical-shift perturbation Kd = 7.0 µM Preparation identity	1–93 Primary evidence Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted. Link interpretation same exact chemical identity and native domain; construct equivalence not assumed
9G94 Chain A	PWWP1 · A1D73 INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N	native annotation core contact Core 4.92 Å Broader 4.92 Å Distance rank 2; multiple chains contact this component	BPP NMR chemical-shift perturbation Kd = 7.0 µM Preparation identity	1–93 Primary evidence Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted. Link interpretation same exact chemical identity and native domain; construct equivalence not assumed
9G94 Chain B	PWWP1 · A1D73 INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N	native annotation core contact Core 2.49 Å Broader 2.49 Å Distance rank 1; multiple chains contact this component	BPP NMR chemical-shift perturbation Kd = 7.0 µM Preparation identity	1–93 Primary evidence Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted. Link interpretation same exact chemical identity and native domain; construct equivalence not assumed
9G94 Chain C	PWWP1 · A1D73 INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N	native annotation core contact Core 2.54 Å Broader 2.54 Å Distance rank 1	BPP NMR chemical-shift perturbation Kd = 7.0 µM Preparation identity	1–93 Primary evidence Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted. Link interpretation same exact chemical identity and native domain; construct equivalence not assumed

HDGFL2 · Additional published evidence

Assays without an exact contact link

3 records

These observations remain usable at their stated preparation and assay level. A negative result, missing co-crystal, unresolved preparation or mixed stereochemistry can prevent an exact contact link.

Preparation	Result	Identity status	Source
H4K20me3 peptide / trimethyllysine monomer	Fluorescence polarization binding Very weak; too weak to fit reliably	defined modified peptide sequence not monomer identity	PMID:21720545 Additional uncertainty not recorded in this snapshot.
H4K20me3 peptide / trimethyllysine monomer	X-ray structural association; no pharmacological activity assigned No source-checked numeric value	defined modified peptide sequence not monomer identity	PMID:21720545 M3L has a polymer residue identity. 3QBY structural occupancy coexists with very weak unfittable solution affinity in Table 1.
NbH10	SPR apparent Kd = 81.2 nM ± 1.8	named macromolecule no full sequence identity	PMID:42194065 Additional uncertainty not recorded in this snapshot.

HDGFL2 · Identity scope

Preparation identities and modeled candidates

4 records

Preparation	Identity status	Exact identity	Modeled candidates
BPP	exact single CCD identity	INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N	[] Complete deposited identity retained; no connectivity-only or name-only merge.
H4K20me3 peptide / trimethyllysine monomer	defined modified peptide sequence not monomer identity	Unresolved at exact-identity level	[] M3L is one covalently linked residue, not the assayed peptide chemical identity.
NbH10	named macromolecule no full sequence identity	Unresolved at exact-identity level	[] Complete deposited identity retained; no connectivity-only or name-only merge.
varenicline	exact single CCD identity	INCHIKEY:JQSHBVHOMNKWFT-DTORHVGOSA-N	[] Complete deposited identity retained; no connectivity-only or name-only merge.