Published assay observation

BPP and HDGFL2

PWWP:ASSAY:0011

direct binding affinity

Reported observation

NMR chemical-shift perturbation Kd

= 7.0 µM ± 5.0

Reported uncertainty: fitting_error

Target domain: UP:Q7Z4V5:PWWP1
Assay construct: 1–93
Identity scope: exact single CCD identity

Evidence provenance

Primary source

PMID:39031937

Results; Figure 1E

Verification scope: primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved

Assay and verification JSON

Field-level provenance

Assay conditions and source verification

Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.

buffer: HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection
pH: 7.4
DMSO percent: Uncurated; field-level source review not performed for this record
temperature C: Uncurated; field-level source review not performed for this record
replicates: Uncurated; field-level source review not performed for this record
uncertainty type: fitting_error

Structure → Component → Assay