{"assay":{"DMSO_percent":null,"abstract_reported_endpoint":null,"abstract_reported_method":null,"abstract_reported_units":null,"abstract_reported_value":null,"assay_construct_end":93,"assay_construct_start":1,"assay_record_id":"PWWP:ASSAY:0011","assay_type":"NMR chemical-shift perturbation Kd","assay_value_transferred_to_modeled_stereoisomers":0,"audit_decision":"retain_with_explicit_endpoint_and_identity","baseline_assay_record":1,"buffer":"HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection","ccd_id":"A1D73","ccd_mapping_source":"Provenance/Literature/ligand_ccd_associations.json","chemical_id":"INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N","chemical_identity_status":"exact_single_CCD_identity","component_context":null,"compound_name":"BPP","conditions":"{\"fit_model\": \"1:1 binding; global fit with shared Kd\", \"ligand_to_protein_molar_ratio_range\": [0, 6], \"protein_concentration_uM\": 100, \"protein_construct\": \"HDGFL2 1\u201393\"}","crystallographic_component_ids":null,"domain":"PWWP","domain_id":"UP:Q7Z4V5:PWWP1","endpoint_label_status":null,"evidence_class":"direct_binding_affinity","evidence_location":"Results; Figure 1E","evidence_url":"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981","gene":"HDGFL2","interpretation":"Authors report intermediate-exchange limitations and additional ITC validation. This record preserves the NMR value and fitting error.","molecular_entity_type":"small_molecule_preparation","numeric_transcription_status":"matches_primary_report","numeric_value_inferred_from_structure":0,"pH":7.4,"pdb_id":"8Z6C","preparation_id":"PREP:BPP","protein_id":"UP:Q7Z4V5","qualitative_result":null,"reference_id":"PMID:39031937","remaining_uncertainty":"Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted.","replicates":null,"role":"experimentally_studied_fragment_ligand","source_component_url":null,"source_id":"S04","source_reported_uncertainty":"5","source_reported_units":"\u00b5M","source_reported_value":"7","source_verification_scope":"primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved","temperature_C":null,"uncertainty":5.0,"uncertainty_type":"fitting_error","uncertainty_uM":5.0,"unit_conversion":null,"units":"\u00b5M","value":7.0,"value_operator":"=","value_uM":7.0,"verification_level":"primary_full_text_sections_verified"},"source_verification":null}