Scientific evidence classification · Phase 6A preserved + Phase 6B additions

PWWP Compounds
& Bioactivity

Explore experimentally studied preparations separately from deposited research ligands, buffers, ions and unresolved chemical evidence. Every role is source- and target-context specific.

Explore studied compounds Structural & Binding Landscape →

Different denominators · 26 October 2026

38studied preparation profiles · 26 baseline + 12 new

109profiles with PWWP research-ligand geometry

93source assay / association observations · 80 baseline + 13 new

Combined Phase 6A + Phase 6B denominators. These groups overlap; the 109 structurally source-tagged research ligands are unchanged. Deposited components and reagents are not automatically biochemical binders.

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Specific PWWP domainExact PWWP-proximal deposited siteChemical identity

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Assay method Endpoint Reported relation Exact concentration min (µM)Exact concentration max (µM) Publication / source databasePublication yearAssay organismTested constructVerification status / scope Missing experimental detailAny associated PDB structure Inventory scopeSort

Assay filters must match the same observation. Concentration ranges require one endpoint and exact positive measurements; inequalities and qualitative findings remain searchable separately.

Phase 6B · source-reviewed additions (separate from the Phase 6A snapshot)

0 matching new observations · 0 preparations

Keep source-level IC50, Kd, and whole-protein DC50 separate; these records were not included in the original 80.

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Preparation	Target scope	Original assay	Primary source
No Phase 6B additions match the selected observation-level filters. This does not erase existing Phase 6A observations.

Evidence classification · exact source records

75 matching profiles

7 matching assay / association observations. Counts here are profiles, not unique assay events or deposited ligand instances; views overlap.

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Preparation	Tested target & construct	Method / endpoint	Original observation	Conditions & uncertainty	Source & verification
BPPPREP:BPP	HDGFL2 PWWP1 1–93Variant: Y18SHomo sapiens	KdNMR chemical-shift perturbation Kddirect binding affinity	= 0.29 ± 0.02 mM290 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting error Temperature °C Buffer Not curated Conditions {'binding_model': '1:1 global fit of perturbed residues', 'buffer': 'HEPES-buffered saline; CSP subsection does not separately give HEPES/NaCl concentrations', 'ligand_protein_molar_ratio_range': [0, 6], 'pH': 7.4, 'protein_concentration_mM': 0.1, 'target_variant': 'Y18S'} Qualification Y18S mutant measurement; must not be pooled with wild-type affinity. Construct is the source HDGFRP2 PWWP expression fragment (1–93) carrying the stated mutation. Replicate number and titration-specific temperature are not independently specified.	PMID:39031937 · 2024Official publisher full HTML independently inspected: Methods, Results and Figures 1–4 captions; original Supplementary Figure S2 was not retrieved Original observation record { "assay_construct_end": 93, "assay_construct_start": 1, "assay_context": "purified_mutant_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0028", "assay_type": "NMR chemical-shift perturbation Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "compound_name": "BPP", "conditions": { "binding_model": "1:1 global fit of perturbed residues", "buffer": "HEPES-buffered saline; CSP subsection does not separately give HEPES/NaCl concentrations", "ligand_protein_molar_ratio_range": [ 0, 6 ], "pH": 7.4, "protein_concentration_mM": 0.1, "target_variant": "Y18S" }, "domain": "PWWP1", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 3D", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.14981", "gene": "HDGFL2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:BPP", "protein_id": "UP:Q7Z4V5", "publication_year": 2024, "reference_id": "PMID:39031937", "remaining_uncertainty": "Y18S mutant measurement; must not be pooled with wild-type affinity. Construct is the source HDGFRP2 PWWP expression fragment (1\u201393) carrying the stated mutation. Replicate number and titration-specific temperature are not independently specified.", "replicates": null, "retrieval_date": "2026-10-26", "role": "experimentally_studied_ligand", "source_id": "P5:S:WEI2024", "source_reported_uncertainty": "0.02", "source_reported_units": "mM", "source_reported_value": "0.29", "source_verification_scope": "Official publisher full HTML independently inspected: Methods, Results and Figures 1\u20134 captions; original Supplementary Figure S2 was not retrieved", "target_variant": "Y18S", "uncertainty": 0.02, "uncertainty_type": "fitting error", "uncertainty_uM": 20.0, "unit_conversion": "mM \u00d7 1000 = \u00b5M", "units": "mM", "value": 0.29, "value_operator": "=", "value_uM": 290.0, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
BPPPREP:BPP	HDGFL2 PWWP 1–93Homo sapiens	KdNMR chemical-shift perturbation Kddirect binding affinity	= 7 ± 5 µM7 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting_error Temperature — Buffer HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection Conditions {"fit_model": "1:1 binding; global fit with shared Kd", "ligand_to_protein_molar_ratio_range": [0, 6], "protein_concentration_uM": 100, "protein_construct": "HDGFL2 1–93"} Qualification Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted.	PMID:39031937 · 2024primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 93, "assay_construct_start": 1, "assay_record_id": "PWWP:ASSAY:0011", "assay_type": "NMR chemical-shift perturbation Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0011\", \"assay_type\": \"NMR chemical-shift perturbation Kd\", \"ccd_id\": \"A1D73\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"BPP\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results; Figure 1E\", \"evidence_url\": \"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981\", \"gene\": \"HDGFL2\", \"interpretation\": \"Authors report intermediate-exchange limitations and additional ITC validation. This record preserves the NMR value and fitting error.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"8Z6C\", \"reference_id\": \"PMID:39031937\", \"role\": \"experimentally_studied_fragment_ligand\", \"uncertainty\": 5, \"units\": \"\u00b5M\", \"value\": 7, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection", "ccd_id": "A1D73", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "BPP", "conditions": "{\"fit_model\": \"1:1 binding; global fit with shared Kd\", \"ligand_to_protein_molar_ratio_range\": [0, 6], \"protein_concentration_uM\": 100, \"protein_construct\": \"HDGFL2 1\u201393\"}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 1E", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981", "gene": "HDGFL2", "interpretation": "Authors report intermediate-exchange limitations and additional ITC validation. This record preserves the NMR value and fitting error.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.4, "pdb_id": "8Z6C", "preparation_id": "PREP:BPP", "protein_id": "UP:Q7Z4V5", "qualitative_result": null, "reference_id": "PMID:39031937", "remaining_uncertainty": "Intermediate-exchange NMR fit has large fitting error; ITC cross-validation reported, exact separate ITC number not extracted.", "replicates": null, "role": "experimentally_studied_fragment_ligand", "source_component_url": null, "source_id": "S04", "source_reported_uncertainty": "5", "source_reported_units": "\u00b5M", "source_reported_value": "7", "source_verification_scope": "primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved", "temperature_C": null, "uncertainty": 5.0, "uncertainty_type": "fitting_error", "uncertainty_uM": 5.0, "unit_conversion": null, "units": "\u00b5M", "value": 7.0, "value_operator": "=", "value_uM": 7.0, "verification_level": "primary_full_text_sections_verified" }
NbH10PREP:NbH10	HDGFL2 PWWP1 1–93Homo sapiens	KdSPR apparent Kddirect binding affinity	= 81.2 ± 1.8 nM0.0812 µM · exact unit conversion	Experimental context Replicates 3 Uncertainty definition SD Temperature 25 °C Buffer 50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20 Conditions {"protein_construct": "HDGFL2 1–93", "replicates": 3, "uncertainty_type": "SD"} Qualification Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody–PWWP complex.	PMID:42194065 · 2026local_primary_full_text_XML_table_and_methods_verified Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 93, "assay_construct_start": 1, "assay_record_id": "PWWP11:ASSAY:0009", "assay_type": "SPR apparent Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": "50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20", "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "named_macromolecule_no_full_sequence_identity", "component_context": null, "compound_name": "NbH10", "conditions": "{\"protein_construct\": \"HDGFL2 1\u201393\", \"replicates\": 3, \"uncertainty_type\": \"SD\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Table 1 NbH10 column; SPR methods", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/", "gene": "HDGFL2", "interpretation": "Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody\u2013PWWP complex.", "molecular_entity_type": "nanobody", "numeric_transcription_status": "matches_primary_table", "numeric_value_inferred_from_structure": null, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:NbH10", "protein_id": "UP:Q7Z4V5", "qualitative_result": null, "reference_id": "PMID:42194065", "remaining_uncertainty": null, "replicates": "3", "role": "macromolecular_binder", "source_component_url": null, "source_id": "S06", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified", "temperature_C": 25, "uncertainty": 1.8, "uncertainty_type": "SD", "uncertainty_uM": 0.0018, "unit_conversion": null, "units": "nM", "value": 81.2, "value_operator": "=", "value_uM": 0.0812, "verification_level": null }
vareniclinePREP:VARENICLINE	HDGFL2 PWWP1 1–93Variant: Y18SHomo sapiens	KdNMR chemical-shift perturbation Kddirect binding affinity	= 0.78 ± 0.05 mM780 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting error Temperature °C Buffer Not curated Conditions {'binding_model': '1:1 global fit of perturbed residues', 'buffer': 'HEPES-buffered saline; CSP subsection does not separately give HEPES/NaCl concentrations', 'ligand_protein_molar_ratio_range': [0, 6], 'pH': 7.4, 'protein_concentration_mM': 0.1, 'target_variant': 'Y18S'} Qualification Y18S mutant measurement; must not be pooled with wild-type affinity. Construct is the source HDGFRP2 PWWP expression fragment (1–93) carrying the stated mutation. Replicate number and titration-specific temperature are not independently specified.	PMID:39031937 · 2024Official publisher full HTML independently inspected: Methods, Results and Figures 1–4 captions; original Supplementary Figure S2 was not retrieved Original observation record { "assay_construct_end": 93, "assay_construct_start": 1, "assay_context": "purified_mutant_domain", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0027", "assay_type": "NMR chemical-shift perturbation Kd", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:JQSHBVHOMNKWFT-DTORHVGOSA-N", "compound_name": "varenicline", "conditions": { "binding_model": "1:1 global fit of perturbed residues", "buffer": "HEPES-buffered saline; CSP subsection does not separately give HEPES/NaCl concentrations", "ligand_protein_molar_ratio_range": [ 0, 6 ], "pH": 7.4, "protein_concentration_mM": 0.1, "target_variant": "Y18S" }, "domain": "PWWP1", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint": "Kd", "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 2E", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.14981", "gene": "HDGFL2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pH": 7.4, "pdb_id": null, "preparation_id": "PREP:VARENICLINE", "protein_id": "UP:Q7Z4V5", "publication_year": 2024, "reference_id": "PMID:39031937", "remaining_uncertainty": "Y18S mutant measurement; must not be pooled with wild-type affinity. Construct is the source HDGFRP2 PWWP expression fragment (1\u201393) carrying the stated mutation. Replicate number and titration-specific temperature are not independently specified.", "replicates": null, "retrieval_date": "2026-10-26", "role": "experimentally_studied_ligand", "source_id": "P5:S:WEI2024", "source_reported_uncertainty": "0.05", "source_reported_units": "mM", "source_reported_value": "0.78", "source_verification_scope": "Official publisher full HTML independently inspected: Methods, Results and Figures 1\u20134 captions; original Supplementary Figure S2 was not retrieved", "target_variant": "Y18S", "uncertainty": 0.05, "uncertainty_type": "fitting error", "uncertainty_uM": 50.0, "unit_conversion": "mM \u00d7 1000 = \u00b5M", "units": "mM", "value": 0.78, "value_operator": "=", "value_uM": 780.0, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }
vareniclinePREP:VARENICLINE	HDGFL2 PWWP 1–93Homo sapiens	KdNMR chemical-shift perturbation Kddirect binding affinity	= 250 ± 40 µM250 µM · exact unit conversion	Experimental context Replicates Not curated Uncertainty definition fitting_error Temperature — Buffer HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection Conditions {"fit_model": "1:1 binding; global fit with shared Kd", "ligand_to_protein_molar_ratio_range": [0, 6], "protein_concentration_uM": 100, "protein_construct": "HDGFL2 1–93"} Qualification Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.	PMID:39031937 · 2024primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": 93, "assay_construct_start": 1, "assay_record_id": "PWWP:ASSAY:0010", "assay_type": "NMR chemical-shift perturbation Kd", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "retain_with_explicit_endpoint_and_identity", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0010\", \"assay_type\": \"NMR chemical-shift perturbation Kd\", \"ccd_id\": \"QMR\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"varenicline\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results; Figure 1E\", \"evidence_url\": \"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981\", \"gene\": \"HDGFL2\", \"interpretation\": \"Converted from 0.25 \u00b1 0.04 mM. Receptor pharmacology in other systems is not evidence of PWWP potency.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"8Z6O\", \"reference_id\": \"PMID:39031937\", \"role\": \"experimentally_studied_fragment_ligand\", \"uncertainty\": 40, \"units\": \"\u00b5M\", \"value\": 250, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": "HEPES-buffered saline; exact buffer/salt concentrations not restated in CSP subsection", "ccd_id": "QMR", "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json", "chemical_id": "INCHIKEY:JQSHBVHOMNKWFT-DTORHVGOSA-N", "chemical_identity_status": "exact_single_CCD_identity", "component_context": null, "compound_name": "varenicline", "conditions": "{\"fit_model\": \"1:1 binding; global fit with shared Kd\", \"ligand_to_protein_molar_ratio_range\": [0, 6], \"protein_concentration_uM\": 100, \"protein_construct\": \"HDGFL2 1\u201393\"}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "direct_binding_affinity", "evidence_location": "Results; Figure 1E", "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981", "gene": "HDGFL2", "interpretation": "Converted from 0.25 \u00b1 0.04 mM. Receptor pharmacology in other systems is not evidence of PWWP potency.", "molecular_entity_type": "small_molecule_preparation", "numeric_transcription_status": "matches_primary_report", "numeric_value_inferred_from_structure": 0, "pH": 7.4, "pdb_id": "8Z6O", "preparation_id": "PREP:VARENICLINE", "protein_id": "UP:Q7Z4V5", "qualitative_result": null, "reference_id": "PMID:39031937", "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.", "replicates": null, "role": "experimentally_studied_fragment_ligand", "source_component_url": null, "source_id": "S04", "source_reported_uncertainty": "0.04", "source_reported_units": "mM", "source_reported_value": "0.25", "source_verification_scope": "primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved", "temperature_C": null, "uncertainty": 40.0, "uncertainty_type": "fitting_error", "uncertainty_uM": 40.0, "unit_conversion": "0.25 mM \u00d7 1000 = 250 \u00b5M; 0.04 mM \u00d7 1000 = 40 \u00b5M", "units": "\u00b5M", "value": 250.0, "value_operator": "=", "value_uM": 250.0, "verification_level": "primary_full_text_sections_verified" }
H4K20me3 peptide / trimethyllysine monomerPREP:H4K20me3_11mer	HDGFL2 PWWP1 Construct not curatedHomo sapiens	Qualitative / associationFluorescence polarization bindingqualitative weak binding	Very weak; too weak to fit reliably	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {"sequence": "AKRHRK(me3)VLRDN"} Qualification Solution binding cannot be quantified from this record; structural occupancy separately linked to 3QBY.	PMID:21720545 · 2011local_primary_full_text_XML_table_verified Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP11:ASSAY:0010", "assay_type": "Fluorescence polarization binding", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": null, "baseline_assay_record": 0, "baseline_original": null, "buffer": null, "ccd_id": null, "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "defined_modified_peptide_sequence_not_monomer_identity", "component_context": null, "compound_name": "H4K20me3 peptide / trimethyllysine monomer", "conditions": "{\"sequence\": \"AKRHRK(me3)VLRDN\"}", "crystallographic_component_ids": null, "domain": "PWWP1", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "qualitative_weak_binding", "evidence_location": "Table 1 H4K20me3 / HDGF2 cell and footnote", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/", "gene": "HDGFL2", "interpretation": "Solution binding cannot be quantified from this record; structural occupancy separately linked to 3QBY.", "molecular_entity_type": "modified_histone_peptide", "numeric_transcription_status": "null_preserved_for_unfittable_binding", "numeric_value_inferred_from_structure": null, "pH": null, "pdb_id": "3QBY", "preparation_id": "PREP:H4K20me3_11mer", "protein_id": "UP:Q7Z4V5", "qualitative_result": "Very weak; too weak to fit reliably", "reference_id": "PMID:21720545", "remaining_uncertainty": null, "replicates": null, "role": "histone_peptide_assay", "source_component_url": null, "source_id": "S07", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML_table_verified", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": null }
H4K20me3 peptide / trimethyllysine monomerPREP:H4K20me3_11mer	HDGFL2 PWWP Construct not curatedHomo sapiens	Qualitative / associationX-ray structural association; no pharmacological activity assignedstructural occupancy association	No quantitative observation curated	Experimental context Replicates Not curated Uncertainty definition Not curated; do not assume SD or SEM Temperature — Buffer Not curated Conditions {} Qualification M3L has a polymer residue identity. 3QBY structural occupancy coexists with very weak unfittable solution affinity in Table 1.	PMID:21720545 · 2011local_primary_full_text_XML; Tables 1-2, crystallization methods and ligand Results inspected Original observation record { "DMSO_percent": null, "abstract_reported_endpoint": null, "abstract_reported_method": null, "abstract_reported_units": null, "abstract_reported_value": null, "assay_construct_end": null, "assay_construct_start": null, "assay_record_id": "PWWP:ASSAY:0036", "assay_type": "X-ray structural association; no pharmacological activity assigned", "assay_value_transferred_to_modeled_stereoisomers": 0, "audit_decision": "separate_whole_peptide_preparation_from_M3L_monomer", "baseline_assay_record": 1, "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0036\", \"assay_type\": \"X-ray structural association; no pharmacological activity assigned\", \"ccd_id\": \"M3L\", \"component_context\": \"nonstandard_polymer_monomer\", \"compound_name\": \"H4K20me3 peptide / trimethyllysine monomer\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Table 2; RCSB polymer entity 2\", \"evidence_url\": \"https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/\", \"gene\": \"HDGFL2\", \"interpretation\": \"Chain H is an 11-residue H4K20me3 peptide AKRHR(M3L)VLRDN. M3L is a modified polymer monomer, not a free drug. The modeled subset must be distinguished from the full deposited peptide.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": \"3QBY\", \"reference_id\": \"PMID:21720545\", \"role\": \"histone methyllysine surrogate/partially resolved peptide\", \"source_component_url\": \"https://data.rcsb.org/rest/v1/core/polymer_entity/3QBY/2\", \"uncertainty\": null, \"units\": null, \"value\": null, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}", "buffer": null, "ccd_id": "M3L", "ccd_mapping_source": null, "chemical_id": null, "chemical_identity_status": "defined_modified_peptide_sequence_not_monomer_identity", "component_context": "nonstandard_polymer_monomer", "compound_name": "H4K20me3 peptide / trimethyllysine monomer", "conditions": "{}", "crystallographic_component_ids": null, "domain": "PWWP", "domain_id": "UP:Q7Z4V5:PWWP1", "endpoint_label_status": null, "evidence_class": "structural_occupancy_association", "evidence_location": "Table 2; RCSB polymer entity 2", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/", "gene": "HDGFL2", "interpretation": "Chain H is an 11-residue H4K20me3 peptide AKRHR(M3L)VLRDN. M3L is a modified polymer monomer, not a free drug. The modeled subset must be distinguished from the full deposited peptide.", "molecular_entity_type": "modified_histone_peptide", "numeric_transcription_status": "not_a_quantitative_affinity_record", "numeric_value_inferred_from_structure": 0, "pH": null, "pdb_id": "3QBY", "preparation_id": "PREP:H4K20me3_11mer", "protein_id": "UP:Q7Z4V5", "qualitative_result": null, "reference_id": "PMID:21720545", "remaining_uncertainty": "M3L has a polymer residue identity. 3QBY structural occupancy coexists with very weak unfittable solution affinity in Table 1.", "replicates": null, "role": "histone methyllysine surrogate/partially resolved peptide", "source_component_url": "https://data.rcsb.org/rest/v1/core/polymer_entity/3QBY/2", "source_id": "S07", "source_reported_uncertainty": null, "source_reported_units": null, "source_reported_value": null, "source_verification_scope": "local_primary_full_text_XML; Tables 1-2, crystallization methods and ligand Results inspected", "temperature_C": null, "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "unit_conversion": null, "units": null, "value": null, "value_operator": null, "value_uM": null, "verification_level": "primary_full_text_sections_verified" }

PWWP Compounds& Bioactivity

0 matching new observations · 0 preparations

75 matching profiles

PWWP Compounds
& Bioactivity