PWWPAtlas
STRUCTURAL BIOLOGY & CHEMICAL EVIDENCEDataset v1.2 · Phase 6D · Comparative experimental recognition environments

Scientific evidence classification · Phase 6A preserved + Phase 6B additions

PWWP Compounds
& Bioactivity

Explore experimentally studied preparations separately from deposited research ligands, buffers, ions and unresolved chemical evidence. Every role is source- and target-context specific.

Explore studied compoundsStructural & Binding Landscape →
Different denominators · 26 October 2026
38studied preparation profiles · 26 baseline + 12 new
109profiles with PWWP research-ligand geometry
93source assay / association observations · 80 baseline + 13 new

Combined Phase 6A + Phase 6B denominators. These groups overlap; the 109 structurally source-tagged research ligands are unchanged. Deposited components and reagents are not automatically biochemical binders.

Assay, source & experimental-context filters

Assay filters must match the same observation. Concentration ranges require one endpoint and exact positive measurements; inequalities and qualitative findings remain searchable separately.

Reset filtersProtein-level observations are never copied to individual PWWP domains.

Phase 6B · source-reviewed additions (separate from the Phase 6A snapshot)

0 matching new observations · 0 preparations

Keep source-level IC50, Kd, and whole-protein DC50 separate; these records were not included in the original 80.

Filter all Phase 6B assay contexts
PreparationTarget scopeOriginal assayPrimary source
No Phase 6B additions match the selected observation-level filters. This does not erase existing Phase 6A observations.

Evidence classification · exact source records

2 matching profiles

2 matching assay / association observations. Counts here are profiles, not unique assay events or deposited ligand instances; views overlap.

Export assay CSVExport evidence JSON
PreparationTested target & constructMethod / endpointOriginal observationConditions & uncertaintySource & verification
BPPPREP:BPP PSIP1 PWWP
Construct not curatedHomo sapiens		 KdITC Kddirect binding affinity = 27 ± 2 µM27 µM · exact unit conversion
Experimental context
Replicates
Not curated
Uncertainty definition
fitting_error
Temperature
—
Buffer
Not curated
Conditions
{}
Qualification
Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.
 PMID:39031937 · 2024primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved
Original observation record
{
  "DMSO_percent": null,
  "abstract_reported_endpoint": null,
  "abstract_reported_method": null,
  "abstract_reported_units": null,
  "abstract_reported_value": null,
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_record_id": "PWWP:ASSAY:0012",
  "assay_type": "ITC Kd",
  "assay_value_transferred_to_modeled_stereoisomers": 0,
  "audit_decision": "retain_with_explicit_endpoint_and_identity",
  "baseline_assay_record": 1,
  "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0012\", \"assay_type\": \"ITC Kd\", \"ccd_id\": \"A1D73\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"BPP\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results; Figure 4B\", \"evidence_url\": \"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981\", \"gene\": \"PSIP1\", \"interpretation\": \"Binding is established in solution; no PSIP1\u2013BPP crystal structure is assigned here.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:39031937\", \"role\": \"experimentally_studied_fragment_ligand\", \"uncertainty\": 2, \"units\": \"\u00b5M\", \"value\": 27, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}",
  "buffer": null,
  "ccd_id": "A1D73",
  "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json",
  "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N",
  "chemical_identity_status": "exact_single_CCD_identity",
  "component_context": null,
  "compound_name": "BPP",
  "conditions": "{}",
  "crystallographic_component_ids": null,
  "domain": "PWWP",
  "domain_id": "UP:O75475:PWWP1",
  "endpoint_label_status": null,
  "evidence_class": "direct_binding_affinity",
  "evidence_location": "Results; Figure 4B",
  "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981",
  "gene": "PSIP1",
  "interpretation": "Binding is established in solution; no PSIP1\u2013BPP crystal structure is assigned here.",
  "molecular_entity_type": "small_molecule_preparation",
  "numeric_transcription_status": "matches_primary_report",
  "numeric_value_inferred_from_structure": 0,
  "pH": null,
  "pdb_id": null,
  "preparation_id": "PREP:BPP",
  "protein_id": "UP:O75475",
  "qualitative_result": null,
  "reference_id": "PMID:39031937",
  "remaining_uncertainty": "Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.",
  "replicates": null,
  "role": "experimentally_studied_fragment_ligand",
  "source_component_url": null,
  "source_id": "S04",
  "source_reported_uncertainty": "2",
  "source_reported_units": "\u00b5M",
  "source_reported_value": "27",
  "source_verification_scope": "primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved",
  "temperature_C": null,
  "uncertainty": 2.0,
  "uncertainty_type": "fitting_error",
  "uncertainty_uM": 2.0,
  "unit_conversion": null,
  "units": "\u00b5M",
  "value": 27.0,
  "value_operator": "=",
  "value_uM": 27.0,
  "verification_level": "primary_full_text_sections_verified"
}
NbH10PREP:NbH10 PSIP1 PWWP1
1–90Homo sapiens		 KdSPR apparent Kddirect binding affinity = 94.4 ± 9.1 nM0.0944 µM · exact unit conversion
Experimental context
Replicates
3
Uncertainty definition
SD
Temperature
25 °C
Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20
Conditions
{"protein_construct": "PSIP1 1–90", "replicates": 3, "uncertainty_type": "SD"}
Qualification
Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody–PWWP complex.
 PMID:42194065 · 2026local_primary_full_text_XML_table_and_methods_verified
Original observation record
{
  "DMSO_percent": null,
  "abstract_reported_endpoint": null,
  "abstract_reported_method": null,
  "abstract_reported_units": null,
  "abstract_reported_value": null,
  "assay_construct_end": 90,
  "assay_construct_start": 1,
  "assay_record_id": "PWWP11:ASSAY:0008",
  "assay_type": "SPR apparent Kd",
  "assay_value_transferred_to_modeled_stereoisomers": 0,
  "audit_decision": null,
  "baseline_assay_record": 0,
  "baseline_original": null,
  "buffer": "50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20",
  "ccd_id": null,
  "ccd_mapping_source": null,
  "chemical_id": null,
  "chemical_identity_status": "named_macromolecule_no_full_sequence_identity",
  "component_context": null,
  "compound_name": "NbH10",
  "conditions": "{\"protein_construct\": \"PSIP1 1\u201390\", \"replicates\": 3, \"uncertainty_type\": \"SD\"}",
  "crystallographic_component_ids": null,
  "domain": "PWWP1",
  "domain_id": "UP:O75475:PWWP1",
  "endpoint_label_status": null,
  "evidence_class": "direct_binding_affinity",
  "evidence_location": "Table 1 NbH10 column; SPR methods",
  "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/",
  "gene": "PSIP1",
  "interpretation": "Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody\u2013PWWP complex.",
  "molecular_entity_type": "nanobody",
  "numeric_transcription_status": "matches_primary_table",
  "numeric_value_inferred_from_structure": null,
  "pH": 7.4,
  "pdb_id": null,
  "preparation_id": "PREP:NbH10",
  "protein_id": "UP:O75475",
  "qualitative_result": null,
  "reference_id": "PMID:42194065",
  "remaining_uncertainty": null,
  "replicates": "3",
  "role": "macromolecular_binder",
  "source_component_url": null,
  "source_id": "S06",
  "source_reported_uncertainty": null,
  "source_reported_units": null,
  "source_reported_value": null,
  "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified",
  "temperature_C": 25,
  "uncertainty": 9.1,
  "uncertainty_type": "SD",
  "uncertainty_uM": 0.0091,
  "unit_conversion": null,
  "units": "nM",
  "value": 94.4,
  "value_operator": "=",
  "value_uM": 0.0944,
  "verification_level": null
}