PWWPAtlas
STRUCTURAL BIOLOGY & CHEMICAL EVIDENCEDataset v1.2 · Phase 6D · Comparative experimental recognition environments

Scientific evidence classification · Phase 6A preserved + Phase 6B additions

PWWP Compounds
& Bioactivity

Explore experimentally studied preparations separately from deposited research ligands, buffers, ions and unresolved chemical evidence. Every role is source- and target-context specific.

Explore studied compoundsStructural & Binding Landscape →
Different denominators · 26 October 2026
38studied preparation profiles · 26 baseline + 12 new
109profiles with PWWP research-ligand geometry
93source assay / association observations · 80 baseline + 13 new

Combined Phase 6A + Phase 6B denominators. These groups overlap; the 109 structurally source-tagged research ligands are unchanged. Deposited components and reagents are not automatically biochemical binders.

Assay, source & experimental-context filters

Assay filters must match the same observation. Concentration ranges require one endpoint and exact positive measurements; inequalities and qualitative findings remain searchable separately.

Reset filtersProtein-level observations are never copied to individual PWWP domains.

Phase 6B · source-reviewed additions (separate from the Phase 6A snapshot)

0 matching new observations · 0 preparations

Keep source-level IC50, Kd, and whole-protein DC50 separate; these records were not included in the original 80.

Filter all Phase 6B assay contexts
PreparationTarget scopeOriginal assayPrimary source
No Phase 6B additions match the selected observation-level filters. This does not erase existing Phase 6A observations.

Evidence classification · exact source records

1 matching profiles

1 matching assay / association observations. Counts here are profiles, not unique assay events or deposited ligand instances; views overlap.

Export assay CSVExport evidence JSON
PreparationTested target & constructMethod / endpointOriginal observationConditions & uncertaintySource & verification
BPPPREP:BPP HDGFL3 PWWP
Construct not curatedHomo sapiens		 KdBinding Kd; specific assay in Figure S2 not independently inspecteddirect binding affinity = 14 ± 2 µM14 µM · exact unit conversion
Experimental context
Replicates
Not curated
Uncertainty definition
fitting_error
Temperature
—
Buffer
Not curated
Conditions
{}
Qualification
14 ± 2 µM confirmed in primary Results; specific Figure S2 assay method remains unresolved.
 PMID:39031937 · 2024primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved
Original observation record
{
  "DMSO_percent": null,
  "abstract_reported_endpoint": null,
  "abstract_reported_method": null,
  "abstract_reported_units": null,
  "abstract_reported_value": null,
  "assay_construct_end": null,
  "assay_construct_start": null,
  "assay_record_id": "PWWP:ASSAY:0013",
  "assay_type": "Binding Kd; specific assay in Figure S2 not independently inspected",
  "assay_value_transferred_to_modeled_stereoisomers": 0,
  "audit_decision": "retain_with_explicit_endpoint_and_identity",
  "baseline_assay_record": 1,
  "baseline_original": "{\"assay_record_id\": \"PWWP:ASSAY:0013\", \"assay_type\": \"Binding Kd; specific assay in Figure S2 not independently inspected\", \"ccd_id\": \"A1D73\", \"ccd_mapping_source\": \"Provenance/Literature/ligand_ccd_associations.json\", \"compound_name\": \"BPP\", \"conditions\": {}, \"domain\": \"PWWP\", \"evidence_location\": \"Results: selectivity paragraph\", \"evidence_url\": \"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981\", \"gene\": \"HDGFL3\", \"interpretation\": \"Numerical result verified in main text; precise supporting-assay details remain to be checked.\", \"numeric_value_inferred_from_structure\": false, \"pdb_id\": null, \"reference_id\": \"PMID:39031937\", \"role\": \"experimentally_studied_fragment_ligand\", \"uncertainty\": 2, \"units\": \"\u00b5M\", \"value\": 14, \"value_operator\": \"=\", \"verification_level\": \"primary_full_text_sections_verified\"}",
  "buffer": null,
  "ccd_id": "A1D73",
  "ccd_mapping_source": "Provenance/Literature/ligand_ccd_associations.json",
  "chemical_id": "INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N",
  "chemical_identity_status": "exact_single_CCD_identity",
  "component_context": null,
  "compound_name": "BPP",
  "conditions": "{}",
  "crystallographic_component_ids": null,
  "domain": "PWWP",
  "domain_id": "UP:Q9Y3E1:PWWP1",
  "endpoint_label_status": null,
  "evidence_class": "direct_binding_affinity",
  "evidence_location": "Results: selectivity paragraph",
  "evidence_url": "https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981",
  "gene": "HDGFL3",
  "interpretation": "Numerical result verified in main text; precise supporting-assay details remain to be checked.",
  "molecular_entity_type": "small_molecule_preparation",
  "numeric_transcription_status": "matches_primary_report",
  "numeric_value_inferred_from_structure": 0,
  "pH": null,
  "pdb_id": null,
  "preparation_id": "PREP:BPP",
  "protein_id": "UP:Q9Y3E1",
  "qualitative_result": null,
  "reference_id": "PMID:39031937",
  "remaining_uncertainty": "14 \u00b1 2 \u00b5M confirmed in primary Results; specific Figure S2 assay method remains unresolved.",
  "replicates": null,
  "role": "experimentally_studied_fragment_ligand",
  "source_component_url": null,
  "source_id": "S04",
  "source_reported_uncertainty": "2",
  "source_reported_units": "\u00b5M",
  "source_reported_value": "14",
  "source_verification_scope": "primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved",
  "temperature_C": null,
  "uncertainty": 2.0,
  "uncertainty_type": "fitting_error",
  "uncertainty_uM": 2.0,
  "unit_conversion": null,
  "units": "\u00b5M",
  "value": 14.0,
  "value_operator": "=",
  "value_uM": 14.0,
  "verification_level": "primary_full_text_sections_verified"
}