9FOE A1IEF
native annotation core contact
same exact chemical identity and native domain; construct equivalence not assumed
Published assay observation
compound 7 (Collie 2025) and NSD2
PWWP:ASSAY:0029
Reported observation
HTRF displacement IC50
= 0.21 µM ± 0.01
Reported uncertainty: SEM
- Target domain
- UP:O96028:PWWP1
- Assay construct
- 208–368
- Identity scope
- exact single CCD identity
Evidence provenance
Primary source
PMID:40959233Table 1
- Verification scope
- Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.
Field-level provenance
Assay conditions and source verification
Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.
| Field | Value and source state | Source and interpretation |
|---|---|---|
| construct bounds | {"end": 368, "numbering": "canonical UniProt inclusive", "start": 208} verified | SV05B · Table S2, PDF page 7Inspected source scopeActual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked |
| tags | N-terminal Avi; C-terminal 6His; biotinylated verified | SV05B · Table S2, PDF page 7Inspected source scopeActual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked |
| engineered mutations | not reported | SV05B · Table S2, PDF page 7 No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit. Inspected source scopeActual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked |
| assay type | HTRF displacement IC50 verified | SV05A · Table 1Inspected source scopeActual primary XML; full Results, Table 1 and footnotes inspected |
| temperature C | not inspected | SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scopeBibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D. |
| buffer | not inspected | SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scopeBibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D. |
| pH | not inspected | SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scopeBibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D. |
| DMSO percent | not inspected | SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scopeBibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D. |
| units | µM verified | SV05A · Table 1Inspected source scopeActual primary XML; full Results, Table 1 and footnotes inspected |
| measurement uncertainty | 0.01 verified | SV05A · Table 1 Reported plus/minus magnitude, in the units of the assay result. Inspected source scopeActual primary XML; full Results, Table 1 and footnotes inspected |
| uncertainty type | SEM verified | SV05A · Table 1 footnote bInspected source scopeActual primary XML; full Results, Table 1 and footnotes inspected |
| replicate definition | {"definition": "replicates; independence/biological level not specified for HTRF in Table 1", "n": "at least 3"} verified | SV05A · Table 1 footnote bInspected source scopeActual primary XML; full Results, Table 1 and footnotes inspected |
| measurement entity | PREP:COLLIE2025_7 verified | SV05A · Table 1 The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity. Inspected source scopeActual primary XML; full Results, Table 1 and footnotes inspected |
| concentration range | not inspected | SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scopeBibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D. |
| incubation and flow | not inspected | SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scopeBibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D. |
| fitting | not inspected | SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scopeBibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D. |
| negative observation | not applicable | SV05A · Table 1Inspected source scopeActual primary XML; full Results, Table 1 and footnotes inspected |
| identity assignment | exact_single_CCD_identity verified | SV05A · Synthesis Results and Table 1 Existing curated exact identity, if present, is retained; this phase does not change chemical nodes. Inspected source scopeActual primary XML; full Results, Table 1 and footnotes inspected |
Structure → Component → Assay