6XCG V01
native annotation core contact
same exact chemical identity and native domain; construct equivalence not assumed
Published assay observation
UNC6934 and NSD2
PWWP:ASSAY:0005
Reported observation
AlphaScreen IC50
= 104.0 nM ± 13.0
Reported uncertainty; definition uncurated
- Target domain
- UP:O96028:PWWP1
- Assay construct
- 208–368
- Identity scope
- exact single CCD identity
Evidence provenance
Primary source
PMID:34782742Discussion; biochemical characterization
- Verification scope
- Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained
Field-level provenance
Assay conditions and source verification
Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.
| Field | Value and source state | Source and interpretation |
|---|---|---|
| construct bounds | {"end": 368, "numbering": "canonical inclusive", "start": 208} verified | SV02A · Methods dCypher nucleosome binding assaysInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| tags | 6His-tagged NSD2-PWWP1 verified | SV02A · Results and dCypher methodsInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| engineered mutations | not reported | SV02A · dCypher methods No substituted construct specified for this assay. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| assay type | AlphaScreen IC50 verified | SV02A · Results and assay-specific MethodsInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| temperature C | 23 verified | SV02A · Methods antagonist testing 23°C applies to 15 min compound/protein and 30 min nucleosome preincubations; bead incubation is described as room temperature. Readout temperature is not separately stated. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| buffer | 150 mM NaCl; 20 mM Tris; 0.01% BSA; 0.01% NP-40; 1 mM DTT (DTT omitted from bead buffer) verified | SV02A · Methods dCypher nucleosome binding assaysInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| pH | 7.5 verified | SV02A · Methods dCypher nucleosome binding assaysInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| DMSO percent | not reported | SV02A · Methods antagonist testing Working compound dilutions are in 2% DMSO but this is not identified as the final full-well concentration. No final percentage is inferred. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| units | nM verified | SV02A · Results and assay-specific MethodsInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| measurement uncertainty | 13.0 verified | SV02A · Results and assay-specific Methods Reported plus/minus magnitude, in the units of the assay result. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| uncertainty type | not reported | SV02A · Results; Figure 2c; statistical analysis The ±13 magnitude is stated, but the inspected assay-specific legend does not explicitly label SD versus SEM. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| replicate definition | Three independent experiments (Figure 2c); each 10-point dose-response in duplicate (Methods) verified | SV02A · Figure 2c and dCypher MethodsInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| measurement entity | PREP:UNC6934 verified | SV02A · Results and assay-specific Methods The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| concentration range | {"endpoint_uM": null, "points": 10, "reported_working_compound_uM": 400} verified | SV02A · Methods antagonist testing 400 uM is a working solution concentration, not a silently assigned final assay concentration. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| incubation and flow | {"bead_incubation_min": 60, "bead_temperature": "room temperature", "compound_protein_preincubation_min": 15, "nucleosome_incubation_min": 30} verified | SV02A · Methods antagonist testingInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| fitting | Apparent IC50 from a 10-point curve spanning plateaus; reported for compounds reducing signal at least 50% verified | SV02A · Methods antagonist testingInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| negative observation | not applicable | SV02A · Results and assay-specific MethodsInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| identity assignment | exact_single_CCD_identity verified | SV02A · Chemical series Results and existing CCD mapping No change to existing identity node or preparation ID. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
Structure → Component → Assay