6XCG V01
native annotation core contact
same exact chemical identity and native domain; construct equivalence not assumed
Published assay observation
UNC6934 and NSD2
PWWP:ASSAY:0004
Reported observation
SPR Kd
= 91.0 nM ± 8.0
Reported uncertainty; definition uncurated
- Target domain
- UP:O96028:PWWP1
- Assay construct
- 208–368
- Identity scope
- exact single CCD identity
Evidence provenance
Primary source
PMID:34782742Results; Figures 1–2
- Verification scope
- Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained
Field-level provenance
Assay conditions and source verification
Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.
| Field | Value and source state | Source and interpretation |
|---|---|---|
| construct bounds | {"end": 368, "numbering": "canonical inclusive", "start": 208} verified | SV02A · Methods Construct and expression; Surface plasmon resonanceInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| tags | N-terminal AviTag; C-terminal polyhistidine; biotinylated verified | SV02A · Methods Construct and expressionInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| engineered mutations | not reported | SV02A · Methods Construct and expression Wild-type probe assay; F266A is a separately described control, not assigned to this row. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| assay type | SPR Kd verified | SV02A · Results and assay-specific MethodsInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| temperature C | not reported | SV02A · Methods Surface plasmon resonance No assay temperature is stated in the inspected paper-specific SPR paragraph or inspected supplement. The cited earlier protocol was not inspected; temperatures from expression, crystallization or other assays are not borrowed. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| buffer | 10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO verified | SV02A · Methods Surface plasmon resonanceInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| pH | 7.4 verified | SV02A · Methods Surface plasmon resonanceInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| DMSO percent | 0.5 verified | SV02A · Methods Surface plasmon resonanceInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| units | nM verified | SV02A · Results and assay-specific MethodsInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| measurement uncertainty | 8.0 verified | SV02A · Results and assay-specific Methods Reported plus/minus magnitude, in the units of the assay result. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| uncertainty type | not reported | SV02A · Results; Figure 2a; statistical analysis The reported 91 ± 8 nM is verified, but the inspected assay-specific legend does not explicitly label the ±8 statistic; the general statistics section permits either SD or SEM. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| replicate definition | Triplicate experiments; independence level not explicitly defined in the SPR figure legend verified | SV02A · Figure 2a; Extended Data Figure 1Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| measurement entity | PREP:UNC6934 verified | SV02A · Results and assay-specific Methods The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| concentration range | {"dilution_factor": 0.33, "highest_reported_method_uM": 2, "points": 5} verified | SV02A · Methods Surface plasmon resonanceInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| incubation and flow | {"chip": "SA", "contact_s": 60, "cycles": "single-cycle", "dissociation_s": 120, "flow_uL_per_min": 75, "immobilization_RU": "approximately 5000"} verified | SV02A · Methods Surface plasmon resonanceInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| fitting | Kinetic and steady-state 1:1 fitting shown for UNC6934 verified | SV02A · Figure 2a and Extended Data Figure 1Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| negative observation | not applicable | SV02A · Results and assay-specific MethodsInspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
| identity assignment | exact_single_CCD_identity verified | SV02A · Chemical series Results and existing CCD mapping No change to existing identity node or preparation ID. Inspected source scopePrimary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected |
Structure → Component → Assay