Published assay observation

MRT866 and NSD2

PWWP:ASSAY:0003

direct binding affinity

Reported observation

SPR Kd

= 349.0 nM ± 19.0

Reported uncertainty; definition uncurated

Target domain: UP:O96028:PWWP1
Assay construct: Uncurated–Uncurated
Identity scope: exact single CCD identity

Evidence provenance

Primary source

PMID:34782742

Results: Discovery of a potent ligand targeting NSD2-PWWP1

Verification scope: Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained

Assay and verification JSON

Field-level provenance

Assay conditions and source verification

Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.

Field	Value and source state	Source and interpretation
construct bounds	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
tags	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
engineered mutations	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
assay type	SPR Kd verified	SV02A · Results and assay-specific Methods Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
temperature C	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
buffer	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
pH	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
DMSO percent	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
units	nM verified	SV02A · Results and assay-specific Methods Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
measurement uncertainty	19.0 verified	SV02A · Results and assay-specific Methods Reported plus/minus magnitude, in the units of the assay result. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
uncertainty type	not reported	SV02A · Results MRT866 Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
replicate definition	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
measurement entity	PREP:MRT866 verified	SV02A · Results and assay-specific Methods The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
concentration range	not inspected	Outside the source scope inspected for this field; no absence claim.
incubation and flow	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
fitting	not reported	SV02A · Results MRT866 and general SPR Methods MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
negative observation	not applicable	SV02A · Results and assay-specific Methods Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
identity assignment	exact_single_CCD_identity verified	SV02A · Chemical series Results and existing CCD mapping No change to existing identity node or preparation ID. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected

Structure → Component → Assay