Published assay observation

compound 6 (Collie 2025) and NSD2

PWWP:ASSAY:0028

biochemical interaction inhibition

Reported observation

HTRF displacement IC50

= 1.24 µM ± 0.08

Reported uncertainty: SEM

Target domain: UP:O96028:PWWP1
Assay construct: 208–368
Identity scope: paper label exact identity unresolved

Evidence provenance

Primary source

PMID:40959233

Table 1

Verification scope: Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.

Assay and verification JSON

Field-level provenance

Assay conditions and source verification

Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.

Field	Value and source state	Source and interpretation
construct bounds	{"end": 368, "numbering": "canonical UniProt inclusive", "start": 208} verified	SV05B · Table S2, PDF page 7 Inspected source scope Actual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked
tags	N-terminal Avi; C-terminal 6His; biotinylated verified	SV05B · Table S2, PDF page 7 Inspected source scope Actual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked
engineered mutations	not reported	SV05B · Table S2, PDF page 7 No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit. Inspected source scope Actual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked
assay type	HTRF displacement IC50 verified	SV05A · Table 1 Inspected source scope Actual primary XML; full Results, Table 1 and footnotes inspected
temperature C	not inspected	SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scope Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.
buffer	not inspected	SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scope Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.
pH	not inspected	SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scope Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.
DMSO percent	not inspected	SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scope Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.
units	µM verified	SV05A · Table 1 Inspected source scope Actual primary XML; full Results, Table 1 and footnotes inspected
measurement uncertainty	0.08 verified	SV05A · Table 1 Reported plus/minus magnitude, in the units of the assay result. Inspected source scope Actual primary XML; full Results, Table 1 and footnotes inspected
uncertainty type	SEM verified	SV05A · Table 1 footnote b Inspected source scope Actual primary XML; full Results, Table 1 and footnotes inspected
replicate definition	{"definition": "replicates; independence/biological level not specified for HTRF in Table 1", "n": 2} verified	SV05A · Table 1 footnote b Inspected source scope Actual primary XML; full Results, Table 1 and footnotes inspected
measurement entity	PREP:COLLIE2025_6 verified	SV05A · Table 1 The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity. Inspected source scope Actual primary XML; full Results, Table 1 and footnotes inspected
concentration range	not inspected	SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scope Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.
incubation and flow	not inspected	SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scope Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.
fitting	not inspected	SV05C · Cited full protocol, Carlino et al 2024; Collie SI PDF page 9 Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred. Inspected source scope Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.
negative observation	not applicable	SV05A · Table 1 Inspected source scope Actual primary XML; full Results, Table 1 and footnotes inspected
identity assignment	paper_label_exact_identity_unresolved verified	SV05A · Synthesis Results and Table 1 Existing curated exact identity, if present, is retained; this phase does not change chemical nodes. Inspected source scope Actual primary XML; full Results, Table 1 and footnotes inspected

Structure → Component → Assay

Exact identity contact links

0 contact links

No exact-identity structural contact is linked to this observation. The preparation-level observation remains available.