Published assay observation

BI-9321 and NSD3

PWWP:ASSAY:0009

cellular interaction response

Reported observation

NanoBRET IC50

= 1.2 µM ± 0.06

Reported uncertainty: SD

Target domain: UP:Q9BZ95:PWWP1
Assay construct: Uncurated–Uncurated
Identity scope: exact single CCD identity

Evidence provenance

Primary source

PMID:31285596

SGC probe developer report: Overview

Verification scope: Phase 3 actual publisher Supplementary Table 7 and official Figure 3/4 captions; selected indexed accepted-manuscript Methods, full PDF unavailable (403); developer identity page

Assay and verification JSON

Field-level provenance

Assay conditions and source verification

Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.

Field	Value and source state	Source and interpretation
construct bounds	not inspected	SV03B · NanoBRET main Methods Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions. Inspected source scope Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review
tags	C-terminal NanoLuc on NSD3-PWWP1; C-terminal HaloTag on histone H3 verified	SV03E · Figure 4e caption Residue bounds remain unspecified at the inspected caption scope. Inspected source scope Actual official publisher figure page retrieved; Figure 4e caption inspected
engineered mutations	not inspected	SV03B · NanoBRET main Methods Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions. Inspected source scope Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review
assay type	NanoBRET IC50 verified	SV03A · Supplementary Table 7, PDF page 11 Inspected source scope Actual publisher supplement PDF; Table 7 visually inspected on PDF page 11
temperature C	not inspected	SV03B · NanoBRET main Methods Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions. Inspected source scope Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review
buffer	not inspected	SV03B · NanoBRET main Methods Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions. Inspected source scope Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review
pH	not inspected	SV03B · NanoBRET main Methods Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions. Inspected source scope Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review
DMSO percent	not inspected	SV03B · NanoBRET main Methods Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions. Inspected source scope Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review
units	µM verified	SV03A · Supplementary Table 7, PDF page 11 Inspected source scope Actual publisher supplement PDF; Table 7 visually inspected on PDF page 11
measurement uncertainty	0.06 verified	SV03A · Supplementary Table 7, PDF page 11 Reported mean ± SD; central value unchanged. Inspected source scope Actual publisher supplement PDF; Table 7 visually inspected on PDF page 11
uncertainty type	SD verified	SV03A · Supplementary Table 7, PDF page 11 Inspected source scope Actual publisher supplement PDF; Table 7 visually inspected on PDF page 11
replicate definition	3 independent replicates; mean ± SD verified	SV03E · Figure 4e caption and SV03A Supplementary Table 7 Inspected source scope Actual official publisher figure page retrieved; Figure 4e caption inspected
measurement entity	PREP:BI-9321 verified	SV03A · Supplementary Table 7, PDF page 11 The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity. Inspected source scope Actual publisher supplement PDF; Table 7 visually inspected on PDF page 11
concentration range	not inspected	SV03B · NanoBRET main Methods Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions. Inspected source scope Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review
incubation and flow	not inspected	SV03B · NanoBRET main Methods Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions. Inspected source scope Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review
fitting	not inspected	SV03B · NanoBRET main Methods Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions. Inspected source scope Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review
negative observation	not applicable	SV03A · Supplementary Table 7, PDF page 11 Inspected source scope Actual publisher supplement PDF; Table 7 visually inspected on PDF page 11
identity assignment	{"ccd_id": "EJE", "chemical_id": "INCHIKEY:WOAOENGFAAUUGT-UHFFFAOYSA-N"} verified	SV03C · Properties and existing CCD mapping Inspected source scope Primary developers confirm exact chemical identity, SPR value and U2OS NanoBRET endpoint

Structure → Component → Assay

Exact identity contact links

1 contact links

6G2O EJE

native annotation core contact

same exact chemical identity and native domain; construct equivalence not assumed