6G2O EJE
native annotation core contact
same exact chemical identity and native domain; construct equivalence not assumed
Published assay observation
BI-9321 and NSD3
PWWP:ASSAY:0008
Reported observation
SPR Kd
= 166.0 nM ± 3.0
Reported uncertainty: SD
- Target domain
- UP:Q9BZ95:PWWP1
- Assay construct
- 263–398
- Identity scope
- exact single CCD identity
Evidence provenance
Primary source
PMID:31285596SGC probe developer report: Overview
- Verification scope
- Phase 3 actual publisher Supplementary Table 7 and official Figure 3/4 captions; selected indexed accepted-manuscript Methods, full PDF unavailable (403); developer identity page
Field-level provenance
Assay conditions and source verification
Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.
| Field | Value and source state | Source and interpretation |
|---|---|---|
| construct bounds | {"end": 398, "numbering": "canonical inclusive", "start": 263} verified | SV03B · NSD3-PWWP1 SPR Kd assay indexed primary passageInspected source scopeSelected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review |
| tags | GST fusion retained for assays according to the indexed protein purification paragraph verified | SV03B · Protein production and purification, indexed primary passage Tag status is supported at the general assay preparation level; no full PDF review. Do not use the 247–398 NMR construct. Inspected source scopeSelected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review |
| engineered mutations | not inspected | Outside the source scope inspected for this field; no absence claim. |
| assay type | SPR Kd verified | SV03A · Supplementary Table 7, PDF page 11Inspected source scopeActual publisher supplement PDF; Table 7 visually inspected on PDF page 11 |
| temperature C | not inspected | SV03B · Selected SPR Kd Methods passage Whole primary Methods were unavailable. The temperature 25°C appears in a separate single-point screen immobilization paragraph and is not transferred to thisKd assay. Inspected source scopeSelected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review |
| buffer | 50 mM Tris; 150 mM NaCl; 1 mM TCEP; 0.005% Tween-20; 2% DMSO verified | SV03B · NSD3-PWWP1 SPR Kd assay indexed primary passageInspected source scopeSelected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review |
| pH | 8.0 verified | SV03B · NSD3-PWWP1 SPR Kd assay indexed primary passageInspected source scopeSelected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review |
| DMSO percent | 2.0 verified | SV03B · NSD3-PWWP1 SPR Kd assay indexed primary passageInspected source scopeSelected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review |
| units | nM verified | SV03A · Supplementary Table 7, PDF page 11Inspected source scopeActual publisher supplement PDF; Table 7 visually inspected on PDF page 11 |
| measurement uncertainty | 3.0 verified | SV03A · Supplementary Table 7, PDF page 11 Reported mean ± SD; central value unchanged. Inspected source scopeActual publisher supplement PDF; Table 7 visually inspected on PDF page 11 |
| uncertainty type | SD verified | SV03A · Supplementary Table 7, PDF page 11Inspected source scopeActual publisher supplement PDF; Table 7 visually inspected on PDF page 11 |
| replicate definition | {"figure_definition": "independent experiments", "publisher_figure_3a_n": 3, "summary": "mean \u00b1 SD", "supplementary_table_7_n": 4} source conflict | SV03D · Figure 3a caption; compare SV03A Supplementary Table 7, PDF page 11 Both are actual primary publisher sources. The figure states three independent experiments while the supplementary table states SPR n=4. Neither count is silently preferred. Inspected source scopeActual official publisher figure page retrieved; Figure 3a caption inspected |
| measurement entity | PREP:BI-9321 verified | SV03A · Supplementary Table 7, PDF page 11 The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity. Inspected source scopeActual publisher supplement PDF; Table 7 visually inspected on PDF page 11 |
| concentration range | not inspected | Outside the source scope inspected for this field; no absence claim. |
| incubation and flow | {"immobilized_RU": "3000\u20134000", "instrument": "Biacore T200", "pre_equilibration": "several hours", "references": "carbonic anhydrase II and blank", "sensor": "CM5"} verified | SV03B · SPR Kd assay, indexed primary passageInspected source scopeSelected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review |
| fitting | Global fit of double-referenced association/dissociation or 1:1 steady-state responses; two flow-cell estimates averaged verified | SV03B · SPR Kd assay and continuation, indexed primary passagesInspected source scopeSelected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review |
| negative observation | not applicable | SV03A · Supplementary Table 7, PDF page 11Inspected source scopeActual publisher supplement PDF; Table 7 visually inspected on PDF page 11 |
| identity assignment | {"ccd_id": "EJE", "chemical_id": "INCHIKEY:WOAOENGFAAUUGT-UHFFFAOYSA-N"} verified | SV03C · Properties and existing CCD mappingInspected source scopePrimary developers confirm exact chemical identity, SPR value and U2OS NanoBRET endpoint |
Structure → Component → Assay