Published assay observation

UNC7145 and NSD2

PWWP:ASSAY:0007

negative binding test

Reported observation

SPR no appreciable binding

No verified numeric result

Target domain: UP:O96028:PWWP1
Assay construct: 208–368
Identity scope: paper label exact identity unresolved

Evidence provenance

Primary source

PMID:34782742

Results: Discovery of a potent ligand targeting NSD2-PWWP1

Verification scope: Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained

Assay and verification JSON

Field-level provenance

Assay conditions and source verification

Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.

Field	Value and source state	Source and interpretation
construct bounds	{"end": 368, "numbering": "canonical inclusive", "start": 208} verified	SV02A · Methods Construct and expression; Surface plasmon resonance Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
tags	N-terminal AviTag; C-terminal polyhistidine; biotinylated verified	SV02A · Methods Construct and expression Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
engineered mutations	not reported	SV02A · Methods Construct and expression Wild-type probe assay; F266A is a separately described control, not assigned to this row. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
assay type	SPR no appreciable binding verified	SV02A · Results and assay-specific Methods Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
temperature C	not reported	SV02A · Methods Surface plasmon resonance No assay temperature is stated in the inspected paper-specific SPR paragraph or inspected supplement. The cited earlier protocol was not inspected; temperatures from expression, crystallization or other assays are not borrowed. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
buffer	10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO verified	SV02A · Methods Surface plasmon resonance Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
pH	7.4 verified	SV02A · Methods Surface plasmon resonance Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
DMSO percent	0.5 verified	SV02A · Methods Surface plasmon resonance Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
units	µM verified	SV02A · Results and assay-specific Methods Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
measurement uncertainty	not applicable	SV02A · Results and assay-specific Methods No quantitative fitted estimate. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
uncertainty type	not applicable	SV02A · Results and assay-specific Methods No quantitative fitted estimate. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
replicate definition	not reported	SV02A · Results and SPR Methods No count specifically assigned to this negative observation. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
measurement entity	PREP:UNC7145 verified	SV02A · Results and assay-specific Methods The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
concentration range	{"methods_maximum_uM": 2, "results_maximum_uM": 20} source conflict	SV02A · Results versus Methods Surface plasmon resonance Results report no appreciable binding up to 20 uM; Methods describe 2 uM as highest concentration for UNC6934/UNC7145. Preserve both; no quantitative Kd bound is inferred. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
incubation and flow	{"chip": "SA", "contact_s": 60, "cycles": "single-cycle", "dissociation_s": 120, "flow_uL_per_min": 75, "immobilization_RU": "approximately 5000"} verified	SV02A · Methods Surface plasmon resonance Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
fitting	Kinetic and steady-state 1:1 fitting shown for UNC6934 verified	SV02A · Figure 2a and Extended Data Figure 1 Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
negative observation	No appreciable binding up to 20 uM (Results); Methods describe a 2 uM maximum source conflict	SV02A · Results and SPR Methods Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected
identity assignment	paper_label_exact_identity_unresolved verified	SV02A · Chemical series Results and existing CCD mapping No change to existing identity node or preparation ID. Inspected source scope Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected

Structure → Component → Assay

Exact identity contact links

0 contact links

No exact-identity structural contact is linked to this observation. The preparation-level observation remains available.