STRUCTURAL BIOLOGY & CHEMICAL EVIDENCEDataset v1.2 · Phase 6D · Comparative experimental recognition environments

Published assay observation

NbH10 and PSIP1

PWWP11:ASSAY:0008

direct binding affinity

Reported observation

SPR apparent Kd

= 94.4 nM ± 9.1

Reported uncertainty: SD

Target domain
UP:O75475:PWWP1
Assay construct
1–90
Identity scope
named macromolecule no full sequence identity

Evidence provenance

Primary source

PMID:42194065

Table 1 NbH10 column; SPR methods

Verification scope
local_primary_full_text_XML_table_and_methods_verified
Assay and verification JSON

Field-level provenance

Assay conditions and source verification

Missing information is not proof of absence. The v1.2 source overlay separates not reported in inspected material, not inspected, not transcribed and not applicable.

buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20
pH
7.4
DMSO percent
Uncurated; field-level source review not performed for this record
temperature C
25
replicates
3
uncertainty type
SD

Structure → Component → Assay

Exact identity contact links

0 contact links
No exact-identity structural contact is linked to this observation. The preparation-level observation remains available.