{
  "version": "1.2",
  "review_date": "2026-10-25",
  "generated_at_utc": "2026-10-26T03:33:36.046840+00:00",
  "scope": "Targeted verification of 32 headline assay records; this is not universal protocol validation, a new experiment, or human scientific approval.",
  "provenance": {
    "baseline_assays_sha256": "16f19b416b0dff929481ab53b0391acbf74237ba94b891d85c5b229207046667",
    "baseline_v1_1_zip_sha256": "4beb6e5f8c43088e2823896bfd176c3f20ba387c5a09bb85a7a860560124bf4a",
    "central_values_changed": false,
    "original_sources_included": false
  },
  "status_vocabulary": {
    "verified": "Positive value supported at the specified source scope.",
    "not_reported": "No value stated within the explicitly inspected relevant source section; does not assert absence from all publications.",
    "not_inspected": "Relevant source or section has not been inspected, including inaccessible or cited inherited protocols.",
    "not_transcribed": "Relevant source content inspected but this particular field not extracted; not used to conceal inaccessible sources.",
    "not_applicable": "Field does not apply to this observation, for example uncertainty on a qualitative result.",
    "source_conflict": "Relevant source statements disagree; both preserved without adjudication."
  },
  "counts": {
    "all_assay_association_records": 46,
    "targeted_records": 32,
    "targeted_original_numeric_records": 24,
    "untargeted_records": 14,
    "original_numeric_records": 31,
    "original_numeric_with_construct_bounds": 23,
    "original_numeric_with_temperature": 15,
    "original_numeric_assay_temperature_verified": 14,
    "original_numeric_preincubation_temperature_verified": 1,
    "temperature_scope_note": "14 Collie SPR records at 25°C plus one UNC6934 AlphaScreen preincubation field at 23°C; the latter is not a verified readout temperature.",
    "targeted_temperature_verified": 22,
    "field_status_counts": {
      "not_reported": 48,
      "verified": 437,
      "not_inspected": 44,
      "not_applicable": 43,
      "source_conflict": 4
    }
  },
  "sources": [
    {
      "source_id": "SV02A",
      "doi": "10.1038/s41589-021-00898-0",
      "title": "A chemical probe targeting the PWWP domain alters NSD2 nucleolar localization",
      "url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/",
      "retrieval_url": "https://www.ncbi.nlm.nih.gov/research/bionlp/RESTful/pmcoa.cgi/BioC_xml/PMC9189931/unicode",
      "scope": "Primary author manuscript full text retrieved; relevant Methods, Results and figure legends inspected",
      "locators": "Construct and expression; Surface plasmon resonance; dCypher nucleosome binding assays; Plasmids; Cell culture; NanoBRET PPI assay; Figures 2 and 4",
      "access": "retrieved_and_inspected",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": "35a17e132f92ea501dd387a3e440f9f107209e85eb1e3aea499cb9f21af516aa"
    },
    {
      "source_id": "SV02B",
      "doi": "10.1038/s41589-021-00898-0",
      "title": "Dilworth et al Supplementary Information",
      "url": "https://media.springernature.com/original/springer-static/esm/art%3A10.1038%2Fs41589-021-00898-0/MediaObjects/41589_2021_898_MOESM27_ESM.pdf",
      "scope": "Supplement PDF retrieved; table/section inventory inspected; chemistry and crystallography identified; no assay-specific SPR temperature found",
      "locators": "Supplementary Tables 1-4 and Supplementary Note",
      "access": "retrieved_relevant_sections_inspected",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": "b6e3409f7d0ec6d67f3078276366233b60c47f82a714616087c0c30ba11dcfa7"
    },
    {
      "source_id": "SV03A",
      "doi": "10.1038/s41589-019-0310-x",
      "title": "BI-9321 Supplementary Information",
      "url": "https://media.springernature.com/original/springer-static/esm/art%3A10.1038%2Fs41589-019-0310-x/MediaObjects/41589_2019_310_MOESM79_ESM.pdf",
      "scope": "Actual publisher supplement PDF; Table 7 visually inspected on PDF page 11",
      "locators": "Supplementary Table 7, PDF page 11",
      "access": "retrieved_and_visually_inspected",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": "08dae1a5ccb72307763072229d859e3fd15667ac0bd335f700d3df84c59c3764"
    },
    {
      "source_id": "SV03B",
      "doi": "10.1038/s41589-019-0310-x",
      "title": "Bottcher et al accepted manuscript at Oxford University Research Archive",
      "url": "https://ora.ox.ac.uk/objects/uuid%3Ac14df0f1-cdc0-4dff-831f-38b218b8317a/files/ma19a7c2144b6df601d4e0bc36bba6caa",
      "scope": "Selected indexed primary Methods passages inspected; direct PDF and repository page retrieval returned HTTP 403; not a complete full-text review",
      "locators": "NSD3-PWWP1 SPR Kd assay; protein production and purification; continuation describing at least three independent experiments",
      "access": "indexed_primary_passages_only_full_pdf_unavailable",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": null
    },
    {
      "source_id": "SV03C",
      "doi": "10.1038/s41589-019-0310-x",
      "title": "BI-9321 primary developer report",
      "url": "https://www.thesgc.org/chemical-probes/bi-9321",
      "scope": "Primary developers confirm exact chemical identity, SPR value and U2OS NanoBRET endpoint",
      "locators": "Overview and Properties",
      "access": "primary_developer_page_inspected",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": null
    },
    {
      "source_id": "SV05A",
      "doi": "10.1021/acsmedchemlett.5c00396",
      "title": "Structural and Molecular Insight into the PWWP1 Domain of NSD2 from the Discovery of Novel Binders Via DNA-Encoded Library Screening",
      "url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/",
      "retrieval_url": "https://www.ebi.ac.uk/europepmc/webservices/rest/PMC12434523/fullTextXML",
      "scope": "Actual primary XML; full Results, Table 1 and footnotes inspected",
      "locators": "Table 1 and notes a/b; synthesis and crystallography Results",
      "access": "retrieved_and_inspected",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": "38df8219c7afc25766ae4da86eecfcb1b5a1128ac90f7f61df0c246ca550ed59"
    },
    {
      "source_id": "SV05B",
      "doi": "10.1021/acsmedchemlett.5c00396",
      "title": "Collie et al Supporting Information",
      "url": "https://pubs.acs.org/doi/suppl/10.1021/acsmedchemlett.5c00396/suppl_file/ml5c00396_si_001.pdf",
      "retrieval_url": "https://www.ebi.ac.uk/europepmc/webservices/rest/PMC12434523/supplementaryFiles",
      "scope": "Actual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked",
      "locators": "PDF page 7 Table S2; PDF page 9 SPR and biochemical assay; PDF page 14 compounds 11-13; PDF page 6 Table S1",
      "access": "retrieved_and_visually_inspected",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": "afd8aec03f1201d7c9932cfdba1d423dff82d57f1eb89485123cee2c834f97a9"
    },
    {
      "source_id": "SV05C",
      "doi": "10.1021/acs.jmedchem.4c00215",
      "title": "Identification of Novel Potent NSD2-PWWP1 Ligands Using Structure-Based Design and Computational Approaches",
      "url": "https://doi.org/10.1021/acs.jmedchem.4c00215",
      "scope": "Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.",
      "locators": "Collie SI reference 6; Carlino main article Experimental details unavailable; Europe PMC core record for PMID 38748070 and NCBI PMC identifier conversion",
      "access": "metadata_inspected_main_protocol_unavailable",
      "review_date": "2026-10-26",
      "full_text_redistributed": false,
      "source_sha256": null,
      "repository_access_outcome": {
        "Europe_PMC": "inPMC=N; inEPMC=N; hasPDF=N; hasSuppl=N; subscription required",
        "NCBI_PMC_ID_converter": "Identifier not found in PMC",
        "ACS_main_article": "HTTP 403",
        "ACS_direct_supporting_PDF": "HTTP 403; actual PDF subsequently retrieved from ACS Figshare"
      },
      "access_receipt": "carlino_protocol_access_receipt.json"
    },
    {
      "source_id": "SVHDGF01",
      "doi": "10.1016/j.jmb.2007.01.010",
      "title": "PWWP module of human hepatoma-derived growth factor forms a domain-swapped dimer with much higher affinity for heparin",
      "url": "https://pubmed.ncbi.nlm.nih.gov/17270212/",
      "scope": "Primary publication abstract and official linked 2NLU deposition title inspected; no new heparin assay curation",
      "locators": "Abstract and PDB 2NLU deposition",
      "access": "primary_abstract_and_deposition",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": null
    },
    {
      "source_id": "SVHDGF02",
      "doi": "10.1038/s41598-017-18510-8",
      "title": "Domain swapping and SMYD1 interactions with the PWWP domain of human hepatoma-derived growth factor",
      "url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC5762634/",
      "retrieval_url": "https://www.ncbi.nlm.nih.gov/research/bionlp/RESTful/pmcoa.cgi/BioC_xml/PMC5762634/unicode",
      "scope": "Actual primary full text; construct, DNA, structure, swapping and source identity descriptions inspected",
      "locators": "Abstract; Table 1; Results and Discussion of swapping; Methods Cloning and plasmid construction; Data availability",
      "access": "retrieved_and_inspected",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": "cfaa4a96a6fa2f17fdd20fd066516249bb35a1ecf76f07b0e08889f4a3989537"
    },
    {
      "source_id": "SV03D",
      "doi": "10.1038/s41589-019-0310-x",
      "title": "BI-9321 publisher Figure 3 caption",
      "url": "https://www.nature.com/articles/s41589-019-0310-x/figures/3",
      "scope": "Actual official publisher figure page retrieved; Figure 3a caption inspected",
      "locators": "Figure 3a caption",
      "access": "retrieved_and_inspected",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": "19e80a9920d40e13592faee7c479b1a64595f2c591d07f6b33ffd20a69ac7eb8"
    },
    {
      "source_id": "SV03E",
      "doi": "10.1038/s41589-019-0310-x",
      "title": "BI-9321 publisher Figure 4 caption",
      "url": "https://www.nature.com/articles/s41589-019-0310-x/figures/4",
      "scope": "Actual official publisher figure page retrieved; Figure 4e caption inspected",
      "locators": "Figure 4e caption",
      "access": "retrieved_and_inspected",
      "review_date": "2026-10-25",
      "full_text_redistributed": false,
      "source_sha256": "04a2d9067b62920f93f7162b07ccaa4a13d9c4c21717082a96e2fb272f5cd8c3"
    },
    {
      "source_id": "SV05D",
      "doi": "10.1021/acs.jmedchem.4c00215.s001",
      "parent_doi": "10.1021/acs.jmedchem.4c00215",
      "title": "Carlino et al Supporting Information",
      "url": "https://acs.figshare.com/articles/journal_contribution/Identification_of_Novel_Potent_NSD2-PWWP1_Ligands_Using_Structure-Based_Design_and_Computational_Approaches/25828829",
      "retrieval_url": "https://ndownloader.figshare.com/files/46350962",
      "scope": "Actual 15-page publisher supporting PDF retrieved from ACS Figshare; page 1 contents visually inspected and complete extracted text searched. The supplement contains NMR, crystallography, FEP/ML, analytical chemistry, NanoBRET tracer data and molecular formula strings, but no HTRF protocol was found. This does not establish absence from the unavailable main article.",
      "locators": "PDF page 1 table of contents; PDF pages 13-14 NanoBRET tracer section; complete text search for HTRF, assay, buffer, incubation and temperature",
      "access": "retrieved_contents_visually_inspected_relevant_protocol_not_found_in_supplement",
      "review_date": "2026-10-26",
      "full_text_redistributed": false,
      "source_sha256": "ad92b87ecd95905f0292de48b77e8e4450f7b536b5c46bfa28125bbf2190343a",
      "publisher_file_name": "jm4c00215_si_001.pdf"
    }
  ],
  "records": [
    {
      "assay_record_id": "PWWP:ASSAY:0003",
      "preparation_id": "PREP:MRT866",
      "compound_name": "MRT866",
      "gene": "NSD2",
      "baseline_source_id": "S02",
      "fields": {
        "construct_bounds": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "tags": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "buffer": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "pH": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "DMSO_percent": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "units": {
          "value": "nM",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 19.0,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866",
          "note": ""
        },
        "replicate_definition": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "measurement_entity": {
          "value": "PREP:MRT866",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": null,
          "status": "not_inspected",
          "source_id": null,
          "locator": null,
          "note": "Outside the source scope inspected for this field; no absence claim."
        },
        "incubation_and_flow": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "fitting": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results MRT866 and general SPR Methods",
          "note": "MRT866 349 ± 19 nM is explicitly reported; assay-specific mapping of this measurement to a construct/protocol is not explicit in the inspected paragraphs. The method names UNC6934 and UNC7145; their conditions are not automatically transferred."
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Chemical series Results and existing CCD mapping",
          "note": "No change to existing identity node or preparation ID."
        }
      },
      "verified_updates": {
        "remaining_uncertainty": "349 ± 19 nM verified in Results; MRT866-specific SPR construct/protocol, replicate definition and uncertainty statistic remain unresolved. Crystallography 211–350 is not assigned as the binding-assay construct.",
        "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0004",
      "preparation_id": "PREP:UNC6934",
      "compound_name": "UNC6934",
      "gene": "NSD2",
      "baseline_source_id": "S02",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical inclusive"
          },
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Construct and expression; Surface plasmon resonance",
          "note": ""
        },
        "tags": {
          "value": "N-terminal AviTag; C-terminal polyhistidine; biotinylated",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Construct and expression",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Methods Construct and expression",
          "note": "Wild-type probe assay; F266A is a separately described control, not assigned to this row."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": "No assay temperature is stated in the inspected paper-specific SPR paragraph or inspected supplement. The cited earlier protocol was not inspected; temperatures from expression, crystallization or other assays are not borrowed."
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": ""
        },
        "pH": {
          "value": 7.4,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.5,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": ""
        },
        "units": {
          "value": "nM",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 8.0,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results; Figure 2a; statistical analysis",
          "note": "The reported 91 ± 8 nM is verified, but the inspected assay-specific legend does not explicitly label the ±8 statistic; the general statistics section permits either SD or SEM."
        },
        "replicate_definition": {
          "value": "Triplicate experiments; independence level not explicitly defined in the SPR figure legend",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Figure 2a; Extended Data Figure 1",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:UNC6934",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "highest_reported_method_uM": 2,
            "points": 5,
            "dilution_factor": 0.33
          },
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": ""
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 75,
            "cycles": "single-cycle",
            "immobilization_RU": "approximately 5000",
            "chip": "SA"
          },
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": ""
        },
        "fitting": {
          "value": "Kinetic and steady-state 1:1 fitting shown for UNC6934",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Figure 2a and Extended Data Figure 1",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Chemical series Results and existing CCD mapping",
          "note": "No change to existing identity node or preparation ID."
        }
      },
      "verified_updates": {
        "assay_construct_start": 208,
        "assay_construct_end": 368,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO",
        "pH": 7.4,
        "DMSO_percent": 0.5,
        "replicates": "3 (triplicate experiments; independence level not specified)",
        "remaining_uncertainty": "SPR temperature and exact uncertainty definition are not specified in the inspected assay-specific source. Triplicate experiments reported. Preparation/assay construct differs from crystallization 211–350.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "phase3_verification": "SV02A",
          "purified_tags": "AviTag/NSD2(208–368)/polyhistidine; biotinylated",
          "source_protocol_highest_concentration_uM": 2
        },
        "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0005",
      "preparation_id": "PREP:UNC6934",
      "compound_name": "UNC6934",
      "gene": "NSD2",
      "baseline_source_id": "S02",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical inclusive"
          },
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods dCypher nucleosome binding assays",
          "note": ""
        },
        "tags": {
          "value": "6His-tagged NSD2-PWWP1",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and dCypher methods",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "dCypher methods",
          "note": "No substituted construct specified for this assay."
        },
        "assay_type": {
          "value": "AlphaScreen IC50",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "temperature_C": {
          "value": 23,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods antagonist testing",
          "note": "23°C applies to 15 min compound/protein and 30 min nucleosome preincubations; bead incubation is described as room temperature. Readout temperature is not separately stated."
        },
        "buffer": {
          "value": "150 mM NaCl; 20 mM Tris; 0.01% BSA; 0.01% NP-40; 1 mM DTT (DTT omitted from bead buffer)",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods dCypher nucleosome binding assays",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods dCypher nucleosome binding assays",
          "note": ""
        },
        "DMSO_percent": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Methods antagonist testing",
          "note": "Working compound dilutions are in 2% DMSO but this is not identified as the final full-well concentration. No final percentage is inferred."
        },
        "units": {
          "value": "nM",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 13.0,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results; Figure 2c; statistical analysis",
          "note": "The ±13 magnitude is stated, but the inspected assay-specific legend does not explicitly label SD versus SEM."
        },
        "replicate_definition": {
          "value": "Three independent experiments (Figure 2c); each 10-point dose-response in duplicate (Methods)",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Figure 2c and dCypher Methods",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:UNC6934",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 10,
            "endpoint_uM": null,
            "reported_working_compound_uM": 400
          },
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods antagonist testing",
          "note": "400 uM is a working solution concentration, not a silently assigned final assay concentration."
        },
        "incubation_and_flow": {
          "value": {
            "compound_protein_preincubation_min": 15,
            "nucleosome_incubation_min": 30,
            "bead_incubation_min": 60,
            "bead_temperature": "room temperature"
          },
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods antagonist testing",
          "note": ""
        },
        "fitting": {
          "value": "Apparent IC50 from a 10-point curve spanning plateaus; reported for compounds reducing signal at least 50%",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods antagonist testing",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Chemical series Results and existing CCD mapping",
          "note": "No change to existing identity node or preparation ID."
        }
      },
      "verified_updates": {
        "assay_construct_start": 208,
        "assay_construct_end": 368,
        "buffer": "150 mM NaCl; 20 mM Tris; 0.01% BSA; 0.01% NP-40; 1 mM DTT (DTT omitted from bead buffer)",
        "pH": 7.5,
        "temperature_C": 23,
        "replicates": "3 independent experiments; each 10-point curve in duplicate",
        "remaining_uncertainty": "23°C is the antagonist preincubation temperature, not a verified readout temperature. Final DMSO fraction and SD-versus-SEM definition for ±13 nM are not explicit in the inspected assay-specific text.",
        "conditions": {
          "phase3_verification": "SV02A",
          "protein_construct": "NSD2 208–368",
          "tag": "6His",
          "temperature_scope": "23°C compound/protein and nucleosome preincubation; bead incubation room temperature",
          "readout": "His-NSD2-PWWP1 binding to H3K36me2 designer nucleosome"
        },
        "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0006",
      "preparation_id": "PREP:UNC6934",
      "compound_name": "UNC6934",
      "gene": "NSD2",
      "baseline_source_id": "S02",
      "fields": {
        "construct_bounds": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Plasmids and NanoBRET PPI Methods",
          "note": "Domain named but residue boundaries are not explicitly specified for the NanoLuc construct in inspected text; biochemical 208–368 is not copied onto a cellular construct."
        },
        "tags": {
          "value": "C-terminal NanoLuc on NSD2-PWWP1; C-terminal HaloTag on histone H3.3",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "NanoBRET PPI Methods",
          "note": ""
        },
        "engineered_mutations": {
          "value": "WT assay; F266A tested separately",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Figure 4d/e and NanoBRET PPI Methods",
          "note": ""
        },
        "assay_type": {
          "value": "NanoBRET PPI half-response",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Cell culture and NanoBRET PPI Methods",
          "note": "Cell culture is at 37°C and 5% CO2; the 20 h assay and plate-reader temperatures are not explicitly restated. Keep culture conditions separate from a scalar assay temperature."
        },
        "buffer": {
          "value": "DMEM/F12 without phenol red; 4% FBS; 100 U/mL penicillin; 100 ug/mL streptomycin",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "NanoBRET PPI Methods",
          "note": ""
        },
        "pH": {
          "value": null,
          "status": "not_inspected",
          "source_id": null,
          "locator": null,
          "note": "Outside the source scope inspected for this field; no absence claim."
        },
        "DMSO_percent": {
          "value": null,
          "status": "not_inspected",
          "source_id": null,
          "locator": null,
          "note": "Outside the source scope inspected for this field; no absence claim."
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.25,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Figure 4d; statistical analysis",
          "note": "±0.25 uM preserved; the inspected specific figure legend does not label its summary statistic."
        },
        "replicate_definition": {
          "value": "3 independent replicates",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Figure 4d",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:UNC6934",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": null,
          "status": "not_inspected",
          "source_id": null,
          "locator": null,
          "note": "Outside the source scope inspected for this field; no absence claim."
        },
        "incubation_and_flow": {
          "value": {
            "compound_treatment_h": 20,
            "readout_after_substrate_min": "within 10",
            "cell_culture_C": 37,
            "CO2_percent": 5
          },
          "status": "verified",
          "source_id": "SV02A",
          "locator": "NanoBRET PPI Methods and Cell culture",
          "note": ""
        },
        "fitting": {
          "value": "Half-response inferred from NanoBRET signal; Results/Figure 4d call EC50, Discussion calls IC50",
          "status": "source_conflict",
          "source_id": "SV02A",
          "locator": "Results/Figure 4d and Discussion",
          "note": "Retain neutral cellular half-response endpoint; do not equate this to direct-binding Kd."
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Chemical series Results and existing CCD mapping",
          "note": "No change to existing identity node or preparation ID."
        }
      },
      "verified_updates": {
        "buffer": "DMEM/F12 without phenol red; 4% FBS; 100 U/mL penicillin; 100 ug/mL streptomycin",
        "replicates": "3 independent replicates",
        "remaining_uncertainty": "NanoLuc construct boundaries, readout temperature and SD-versus-SEM are not explicit in the inspected source. Results say EC50 while Discussion says IC50; neutral half-response label retained.",
        "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0007",
      "preparation_id": "PREP:UNC7145",
      "compound_name": "UNC7145",
      "gene": "NSD2",
      "baseline_source_id": "S02",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical inclusive"
          },
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Construct and expression; Surface plasmon resonance",
          "note": ""
        },
        "tags": {
          "value": "N-terminal AviTag; C-terminal polyhistidine; biotinylated",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Construct and expression",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Methods Construct and expression",
          "note": "Wild-type probe assay; F266A is a separately described control, not assigned to this row."
        },
        "assay_type": {
          "value": "SPR no appreciable binding",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": "No assay temperature is stated in the inspected paper-specific SPR paragraph or inspected supplement. The cited earlier protocol was not inspected; temperatures from expression, crystallization or other assays are not borrowed."
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": ""
        },
        "pH": {
          "value": 7.4,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.5,
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "No quantitative fitted estimate."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "No quantitative fitted estimate."
        },
        "replicate_definition": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV02A",
          "locator": "Results and SPR Methods",
          "note": "No count specifically assigned to this negative observation."
        },
        "measurement_entity": {
          "value": "PREP:UNC7145",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Results and assay-specific Methods",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "results_maximum_uM": 20,
            "methods_maximum_uM": 2
          },
          "status": "source_conflict",
          "source_id": "SV02A",
          "locator": "Results versus Methods Surface plasmon resonance",
          "note": "Results report no appreciable binding up to 20 uM; Methods describe 2 uM as highest concentration for UNC6934/UNC7145. Preserve both; no quantitative Kd bound is inferred."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 75,
            "cycles": "single-cycle",
            "immobilization_RU": "approximately 5000",
            "chip": "SA"
          },
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Methods Surface plasmon resonance",
          "note": ""
        },
        "fitting": {
          "value": "Kinetic and steady-state 1:1 fitting shown for UNC6934",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Figure 2a and Extended Data Figure 1",
          "note": ""
        },
        "negative_observation": {
          "value": "No appreciable binding up to 20 uM (Results); Methods describe a 2 uM maximum",
          "status": "source_conflict",
          "source_id": "SV02A",
          "locator": "Results and SPR Methods",
          "note": ""
        },
        "identity_assignment": {
          "value": "paper_label_exact_identity_unresolved",
          "status": "verified",
          "source_id": "SV02A",
          "locator": "Chemical series Results and existing CCD mapping",
          "note": "No change to existing identity node or preparation ID."
        }
      },
      "verified_updates": {
        "assay_construct_start": 208,
        "assay_construct_end": 368,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.05% Tween-20; 0.5% DMSO",
        "pH": 7.4,
        "DMSO_percent": 0.5,
        "remaining_uncertainty": "Primary-source concentration discrepancy: Results report no appreciable binding up to 20 uM, but SPR Methods state 2 uM as highest concentration. Neither is converted into a Kd bound; human source review required.",
        "conditions": {
          "highest_tested_concentration_uM": 20,
          "phase3_verification": "SV02A",
          "purified_tags": "AviTag/NSD2(208–368)/polyhistidine; biotinylated",
          "source_protocol_highest_concentration_uM": 2
        },
        "source_verification_scope": "Phase 3 primary full-text Methods, Results and figure legends through official NCBI BioC; actual publisher supplement; assay-specific scope retained"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0008",
      "preparation_id": "PREP:BI-9321",
      "compound_name": "BI-9321",
      "gene": "NSD3",
      "baseline_source_id": "S03",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 263,
            "end": 398,
            "numbering": "canonical inclusive"
          },
          "status": "verified",
          "source_id": "SV03B",
          "locator": "NSD3-PWWP1 SPR Kd assay indexed primary passage",
          "note": ""
        },
        "tags": {
          "value": "GST fusion retained for assays according to the indexed protein purification paragraph",
          "status": "verified",
          "source_id": "SV03B",
          "locator": "Protein production and purification, indexed primary passage",
          "note": "Tag status is supported at the general assay preparation level; no full PDF review. Do not use the 247–398 NMR construct."
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_inspected",
          "source_id": null,
          "locator": null,
          "note": "Outside the source scope inspected for this field; no absence claim."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "Selected SPR Kd Methods passage",
          "note": "Whole primary Methods were unavailable. The temperature 25°C appears in a separate single-point screen immobilization paragraph and is not transferred to thisKd assay."
        },
        "buffer": {
          "value": "50 mM Tris; 150 mM NaCl; 1 mM TCEP; 0.005% Tween-20; 2% DMSO",
          "status": "verified",
          "source_id": "SV03B",
          "locator": "NSD3-PWWP1 SPR Kd assay indexed primary passage",
          "note": ""
        },
        "pH": {
          "value": 8.0,
          "status": "verified",
          "source_id": "SV03B",
          "locator": "NSD3-PWWP1 SPR Kd assay indexed primary passage",
          "note": ""
        },
        "DMSO_percent": {
          "value": 2.0,
          "status": "verified",
          "source_id": "SV03B",
          "locator": "NSD3-PWWP1 SPR Kd assay indexed primary passage",
          "note": ""
        },
        "units": {
          "value": "nM",
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 3.0,
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": "Reported mean ± SD; central value unchanged."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": ""
        },
        "replicate_definition": {
          "value": {
            "publisher_figure_3a_n": 3,
            "supplementary_table_7_n": 4,
            "figure_definition": "independent experiments",
            "summary": "mean ± SD"
          },
          "status": "source_conflict",
          "source_id": "SV03D",
          "locator": "Figure 3a caption; compare SV03A Supplementary Table 7, PDF page 11",
          "note": "Both are actual primary publisher sources. The figure states three independent experiments while the supplementary table states SPR n=4. Neither count is silently preferred."
        },
        "measurement_entity": {
          "value": "PREP:BI-9321",
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": null,
          "status": "not_inspected",
          "source_id": null,
          "locator": null,
          "note": "Outside the source scope inspected for this field; no absence claim."
        },
        "incubation_and_flow": {
          "value": {
            "instrument": "Biacore T200",
            "sensor": "CM5",
            "immobilized_RU": "3000–4000",
            "references": "carbonic anhydrase II and blank",
            "pre_equilibration": "several hours"
          },
          "status": "verified",
          "source_id": "SV03B",
          "locator": "SPR Kd assay, indexed primary passage",
          "note": ""
        },
        "fitting": {
          "value": "Global fit of double-referenced association/dissociation or 1:1 steady-state responses; two flow-cell estimates averaged",
          "status": "verified",
          "source_id": "SV03B",
          "locator": "SPR Kd assay and continuation, indexed primary passages",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "chemical_id": "INCHIKEY:WOAOENGFAAUUGT-UHFFFAOYSA-N",
            "ccd_id": "EJE"
          },
          "status": "verified",
          "source_id": "SV03C",
          "locator": "Properties and existing CCD mapping",
          "note": ""
        }
      },
      "verified_updates": {
        "uncertainty": 3.0,
        "source_reported_uncertainty": 3.0,
        "uncertainty_uM": 0.003,
        "uncertainty_type": "SD",
        "replicates": "3 independent experiments (Figure 3a); 4 (Supplementary Table 7); unresolved source discrepancy",
        "assay_construct_start": 263,
        "assay_construct_end": 398,
        "buffer": "50 mM Tris; 150 mM NaCl; 1 mM TCEP; 0.005% Tween-20; 2% DMSO",
        "pH": 8.0,
        "DMSO_percent": 2.0,
        "remaining_uncertainty": "Primary replicate conflict: Figure 3a states n=3 independent experiments, while Supplementary Table 7 states SPR n=4; both report SD. Indexed primary SPR Methods supply residues 263–398 and buffer, but the full PDF was unavailable. Exact assay temperature, mutations and per-dose contact times remain not inspected.",
        "source_verification_scope": "Phase 3 actual publisher Supplementary Table 7 and official Figure 3/4 captions; selected indexed accepted-manuscript Methods, full PDF unavailable (403); developer identity page"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0009",
      "preparation_id": "PREP:BI-9321",
      "compound_name": "BI-9321",
      "gene": "NSD3",
      "baseline_source_id": "S03",
      "fields": {
        "construct_bounds": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "NanoBRET main Methods",
          "note": "Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions."
        },
        "tags": {
          "value": "C-terminal NanoLuc on NSD3-PWWP1; C-terminal HaloTag on histone H3",
          "status": "verified",
          "source_id": "SV03E",
          "locator": "Figure 4e caption",
          "note": "Residue bounds remain unspecified at the inspected caption scope."
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "NanoBRET main Methods",
          "note": "Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions."
        },
        "assay_type": {
          "value": "NanoBRET IC50",
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "NanoBRET main Methods",
          "note": "Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions."
        },
        "buffer": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "NanoBRET main Methods",
          "note": "Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions."
        },
        "pH": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "NanoBRET main Methods",
          "note": "Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions."
        },
        "DMSO_percent": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "NanoBRET main Methods",
          "note": "Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions."
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.06,
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": "Reported mean ± SD; central value unchanged."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": ""
        },
        "replicate_definition": {
          "value": "3 independent replicates; mean ± SD",
          "status": "verified",
          "source_id": "SV03E",
          "locator": "Figure 4e caption and SV03A Supplementary Table 7",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:BI-9321",
          "status": "verified",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "NanoBRET main Methods",
          "note": "Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions."
        },
        "incubation_and_flow": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "NanoBRET main Methods",
          "note": "Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions."
        },
        "fitting": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV03B",
          "locator": "NanoBRET main Methods",
          "note": "Full primary NanoBRET protocol was not retrieved. Supplementary Table 7 validates the endpoint, value, error and n, but does not provide complete conditions."
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV03A",
          "locator": "Supplementary Table 7, PDF page 11",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "chemical_id": "INCHIKEY:WOAOENGFAAUUGT-UHFFFAOYSA-N",
            "ccd_id": "EJE"
          },
          "status": "verified",
          "source_id": "SV03C",
          "locator": "Properties and existing CCD mapping",
          "note": ""
        }
      },
      "verified_updates": {
        "uncertainty": 0.06,
        "source_reported_uncertainty": 0.06,
        "uncertainty_uM": 0.06,
        "uncertainty_type": "SD",
        "replicates": "3 independent replicates (Figure 4e; also Supplementary Table 7)",
        "remaining_uncertainty": "The value 1.2 ± 0.06 uM, SD and n=3 were verified in the actual Supplementary Table 7. Full NanoBRET construct and conditions were not inspected. The 1.4 uM direct tracer-engagement endpoint is separate from this histone PPI assay.",
        "source_verification_scope": "Phase 3 actual publisher Supplementary Table 7 and official Figure 3/4 captions; selected indexed accepted-manuscript Methods, full PDF unavailable (403); developer identity page"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0014",
      "preparation_id": "PREP:UNC6934",
      "compound_name": "UNC6934",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.03,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:UNC6934",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0015",
      "preparation_id": "PREP:COLLIE2025_5",
      "compound_name": "compound 5 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.03,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_5",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "assayed_stereoisomeric_mixture_not_single_chemical_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0016",
      "preparation_id": "PREP:COLLIE2025_6",
      "compound_name": "compound 6 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.09,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_6",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "paper_label_exact_identity_unresolved",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0017",
      "preparation_id": "PREP:COLLIE2025_7",
      "compound_name": "compound 7 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.02,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_7",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0018",
      "preparation_id": "PREP:COLLIE2025_11",
      "compound_name": "compound 11 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.19,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_11",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "level": "preparation",
            "fixed_center": "pyrrolidine 2S",
            "unresolved_center": "racemic sulfolane center",
            "candidate_ccd_ids": [
              "A1IEG",
              "A1IFS"
            ],
            "affinity_transfer_allowed": false
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compound 11 and compounds 12/13 chemistry, PDF page 14",
          "note": "The fixed 2S center plus variable sulfolane center defines a diastereomeric preparation. No bulk mixture ratio or assignment of separated fractions to either deposited CCD is established."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0019",
      "preparation_id": "PREP:COLLIE2025_12",
      "compound_name": "compound 12 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.13,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_12",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "level": "source-labelled separated fraction",
            "isomer_label": 1,
            "retention_time_min": 7.1,
            "reported_diastereomeric_ratio": "98.84:1.16",
            "absolute_sulfolane_configuration": null,
            "exact_ccd_id": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compounds 12/13, PDF page 14",
          "note": "Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0020",
      "preparation_id": "PREP:COLLIE2025_13",
      "compound_name": "compound 13 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.04,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_13",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "level": "source-labelled separated fraction",
            "isomer_label": 2,
            "retention_time_min": 8.2,
            "reported_diastereomeric_ratio": "97.10:2.90",
            "absolute_sulfolane_configuration": null,
            "exact_ccd_id": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compounds 12/13, PDF page 14",
          "note": "Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0021",
      "preparation_id": "PREP:UNC6934",
      "compound_name": "UNC6934",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 211,
            "end": 350,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.19,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:UNC6934",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 211–350",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0022",
      "preparation_id": "PREP:COLLIE2025_5",
      "compound_name": "compound 5 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 211,
            "end": 350,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.03,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_5",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "assayed_stereoisomeric_mixture_not_single_chemical_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 211–350",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0023",
      "preparation_id": "PREP:COLLIE2025_6",
      "compound_name": "compound 6 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 211,
            "end": 350,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.64,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_6",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "paper_label_exact_identity_unresolved",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 211–350",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0024",
      "preparation_id": "PREP:COLLIE2025_7",
      "compound_name": "compound 7 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 211,
            "end": 350,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.01,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_7",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 211–350",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0025",
      "preparation_id": "PREP:COLLIE2025_11",
      "compound_name": "compound 11 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 211,
            "end": 350,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.31,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_11",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "level": "preparation",
            "fixed_center": "pyrrolidine 2S",
            "unresolved_center": "racemic sulfolane center",
            "candidate_ccd_ids": [
              "A1IEG",
              "A1IFS"
            ],
            "affinity_transfer_allowed": false
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compound 11 and compounds 12/13 chemistry, PDF page 14",
          "note": "The fixed 2S center plus variable sulfolane center defines a diastereomeric preparation. No bulk mixture ratio or assignment of separated fractions to either deposited CCD is established."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 211–350",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0026",
      "preparation_id": "PREP:COLLIE2025_12",
      "compound_name": "compound 12 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 211,
            "end": 350,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.31,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_12",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "level": "source-labelled separated fraction",
            "isomer_label": 1,
            "retention_time_min": 7.1,
            "reported_diastereomeric_ratio": "98.84:1.16",
            "absolute_sulfolane_configuration": null,
            "exact_ccd_id": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compounds 12/13, PDF page 14",
          "note": "Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 211–350",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0027",
      "preparation_id": "PREP:COLLIE2025_13",
      "compound_name": "compound 13 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 211,
            "end": 350,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.05,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SD",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_13",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "level": "source-labelled separated fraction",
            "isomer_label": 2,
            "retention_time_min": 8.2,
            "reported_diastereomeric_ratio": "97.10:2.90",
            "absolute_sulfolane_configuration": null,
            "exact_ccd_id": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compounds 12/13, PDF page 14",
          "note": "Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "uncertainty_type": "SD",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD2 211–350",
          "replicates": "at least 3",
          "uncertainty_type": "SD",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal GS remnant; C-terminal Avi; biotinylated after GST/6His removal",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0028",
      "preparation_id": "PREP:COLLIE2025_6",
      "compound_name": "compound 6 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "HTRF displacement IC50",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "buffer": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "pH": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "DMSO_percent": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.08,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SEM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote b",
          "note": ""
        },
        "replicate_definition": {
          "value": {
            "n": 2,
            "definition": "replicates; independence/biological level not specified for HTRF in Table 1"
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote b",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_6",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "incubation_and_flow": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "fitting": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "paper_label_exact_identity_unresolved",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": 2,
          "uncertainty_type": "SEM",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts."
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0029",
      "preparation_id": "PREP:COLLIE2025_7",
      "compound_name": "compound 7 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "HTRF displacement IC50",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "buffer": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "pH": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "DMSO_percent": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.01,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SEM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote b",
          "note": ""
        },
        "replicate_definition": {
          "value": {
            "n": "at least 3",
            "definition": "replicates; independence/biological level not specified for HTRF in Table 1"
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote b",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_7",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "incubation_and_flow": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "fitting": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": "at least 3",
          "uncertainty_type": "SEM",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts."
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0030",
      "preparation_id": "PREP:COLLIE2025_12",
      "compound_name": "compound 12 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "HTRF displacement IC50",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "buffer": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "pH": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "DMSO_percent": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.06,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SEM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote b",
          "note": ""
        },
        "replicate_definition": {
          "value": {
            "n": 2,
            "definition": "replicates; independence/biological level not specified for HTRF in Table 1"
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote b",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_12",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "incubation_and_flow": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "fitting": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "level": "source-labelled separated fraction",
            "isomer_label": 1,
            "retention_time_min": 7.1,
            "reported_diastereomeric_ratio": "98.84:1.16",
            "absolute_sulfolane_configuration": null,
            "exact_ccd_id": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compounds 12/13, PDF page 14",
          "note": "Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment."
        }
      },
      "verified_updates": {
        "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": 2,
          "uncertainty_type": "SEM",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts."
      }
    },
    {
      "assay_record_id": "PWWP:ASSAY:0031",
      "preparation_id": "PREP:COLLIE2025_13",
      "compound_name": "compound 13 (Collie 2025)",
      "gene": "NSD2",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 208,
            "end": 368,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal Avi; C-terminal 6His; biotinylated",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "HTRF displacement IC50",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "buffer": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "pH": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "DMSO_percent": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": 0.06,
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Reported plus/minus magnitude, in the units of the assay result."
        },
        "uncertainty_type": {
          "value": "SEM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote b",
          "note": ""
        },
        "replicate_definition": {
          "value": {
            "n": "at least 3",
            "definition": "replicates; independence/biological level not specified for HTRF in Table 1"
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote b",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_13",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "incubation_and_flow": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "fitting": {
          "value": null,
          "status": "not_inspected",
          "source_id": "SV05C",
          "locator": "Cited full protocol, Carlino et al 2024; Collie SI PDF page 9",
          "note": "Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred."
        },
        "negative_observation": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "identity_assignment": {
          "value": {
            "level": "source-labelled separated fraction",
            "isomer_label": 2,
            "retention_time_min": 8.2,
            "reported_diastereomeric_ratio": "97.10:2.90",
            "absolute_sulfolane_configuration": null,
            "exact_ccd_id": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compounds 12/13, PDF page 14",
          "note": "Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment."
        }
      },
      "verified_updates": {
        "remaining_uncertainty": "HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.",
        "conditions": {
          "protein_construct": "NSD2 208–368",
          "replicates": "at least 3",
          "uncertainty_type": "SEM",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal Avi; C-terminal 6His; biotinylated",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts."
      }
    },
    {
      "assay_record_id": "PWWP11:ASSAY:0001",
      "preparation_id": "PREP:UNC6934",
      "compound_name": "UNC6934",
      "gene": "NSD3",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 263,
            "end": 398,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal 6His-TEV retained",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd counter-screen",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Censored threshold is not a point estimate with an error bar."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:UNC6934",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": {
            "operator": ">",
            "value": 30,
            "units": "uM",
            "endpoint": "Kd"
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": "Explicit censored affinity bound; preserve operator and do not treat as a point Kd."
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD3 263–398",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal 6His-TEV retained",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP11:ASSAY:0002",
      "preparation_id": "PREP:COLLIE2025_5",
      "compound_name": "compound 5 (Collie 2025)",
      "gene": "NSD3",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 263,
            "end": 398,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal 6His-TEV retained",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd counter-screen",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Censored threshold is not a point estimate with an error bar."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_5",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": {
            "operator": ">",
            "value": 30,
            "units": "uM",
            "endpoint": "Kd"
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": "Explicit censored affinity bound; preserve operator and do not treat as a point Kd."
        },
        "identity_assignment": {
          "value": "assayed_stereoisomeric_mixture_not_single_chemical_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD3 263–398",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal 6His-TEV retained",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP11:ASSAY:0003",
      "preparation_id": "PREP:COLLIE2025_6",
      "compound_name": "compound 6 (Collie 2025)",
      "gene": "NSD3",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 263,
            "end": 398,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal 6His-TEV retained",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd counter-screen",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Censored threshold is not a point estimate with an error bar."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_6",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": {
            "operator": ">",
            "value": 30,
            "units": "uM",
            "endpoint": "Kd"
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": "Explicit censored affinity bound; preserve operator and do not treat as a point Kd."
        },
        "identity_assignment": {
          "value": "paper_label_exact_identity_unresolved",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD3 263–398",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal 6His-TEV retained",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP11:ASSAY:0004",
      "preparation_id": "PREP:COLLIE2025_7",
      "compound_name": "compound 7 (Collie 2025)",
      "gene": "NSD3",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 263,
            "end": 398,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal 6His-TEV retained",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd counter-screen",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": "µM",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "measurement_uncertainty": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Censored threshold is not a point estimate with an error bar."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_7",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": {
            "operator": ">",
            "value": 30,
            "units": "uM",
            "endpoint": "Kd"
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": "Explicit censored affinity bound; preserve operator and do not treat as a point Kd."
        },
        "identity_assignment": {
          "value": "exact_single_CCD_identity",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Synthesis Results and Table 1",
          "note": "Existing curated exact identity, if present, is retained; this phase does not change chemical nodes."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD3 263–398",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal 6His-TEV retained",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP11:ASSAY:0005",
      "preparation_id": "PREP:COLLIE2025_11",
      "compound_name": "compound 11 (Collie 2025)",
      "gene": "NSD3",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 263,
            "end": 398,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal 6His-TEV retained",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd counter-screen",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Qualitative no-binding observations do not carry numerical units."
        },
        "measurement_uncertainty": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "No quantitative fitted estimate."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_11",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": {
            "label": "No binding",
            "numeric_threshold": null
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": "Qualitative source wording. No numerical threshold is inferred."
        },
        "identity_assignment": {
          "value": {
            "level": "preparation",
            "fixed_center": "pyrrolidine 2S",
            "unresolved_center": "racemic sulfolane center",
            "candidate_ccd_ids": [
              "A1IEG",
              "A1IFS"
            ],
            "affinity_transfer_allowed": false
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compound 11 and compounds 12/13 chemistry, PDF page 14",
          "note": "The fixed 2S center plus variable sulfolane center defines a diastereomeric preparation. No bulk mixture ratio or assignment of separated fractions to either deposited CCD is established."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD3 263–398",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal 6His-TEV retained",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP11:ASSAY:0006",
      "preparation_id": "PREP:COLLIE2025_12",
      "compound_name": "compound 12 (Collie 2025)",
      "gene": "NSD3",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 263,
            "end": 398,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal 6His-TEV retained",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd counter-screen",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Qualitative no-binding observations do not carry numerical units."
        },
        "measurement_uncertainty": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "No quantitative fitted estimate."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_12",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": {
            "label": "No binding",
            "numeric_threshold": null
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": "Qualitative source wording. No numerical threshold is inferred."
        },
        "identity_assignment": {
          "value": {
            "level": "source-labelled separated fraction",
            "isomer_label": 1,
            "retention_time_min": 7.1,
            "reported_diastereomeric_ratio": "98.84:1.16",
            "absolute_sulfolane_configuration": null,
            "exact_ccd_id": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compounds 12/13, PDF page 14",
          "note": "Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD3 263–398",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal 6His-TEV retained",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    },
    {
      "assay_record_id": "PWWP11:ASSAY:0007",
      "preparation_id": "PREP:COLLIE2025_13",
      "compound_name": "compound 13 (Collie 2025)",
      "gene": "NSD3",
      "baseline_source_id": "S05",
      "fields": {
        "construct_bounds": {
          "value": {
            "start": 263,
            "end": 398,
            "numbering": "canonical UniProt inclusive"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "tags": {
          "value": "N-terminal 6His-TEV retained",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": ""
        },
        "engineered_mutations": {
          "value": null,
          "status": "not_reported",
          "source_id": "SV05B",
          "locator": "Table S2, PDF page 7",
          "note": "No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit."
        },
        "assay_type": {
          "value": "SPR Kd counter-screen",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": ""
        },
        "temperature_C": {
          "value": 25,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "buffer": {
          "value": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "pH": {
          "value": 7.5,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "DMSO_percent": {
          "value": 0.3,
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "units": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "Qualitative no-binding observations do not carry numerical units."
        },
        "measurement_uncertainty": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "No quantitative fitted estimate."
        },
        "uncertainty_type": {
          "value": null,
          "status": "not_applicable",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": ""
        },
        "replicate_definition": {
          "value": "At least 3 independent replicates; numeric Kd summaries are mean and SD. The footnote supplies no separate exact n for individual negative/censored cells.",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 footnote a",
          "note": ""
        },
        "measurement_entity": {
          "value": "PREP:COLLIE2025_13",
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1",
          "note": "The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity."
        },
        "concentration_range": {
          "value": {
            "points": 12,
            "dilution_factor": 0.5,
            "maximum_uM": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": "Twofold series; endpoint not stated in inspected protocol. A >30 uM reported Kd bound is not reinterpreted as the assay maximum."
        },
        "incubation_and_flow": {
          "value": {
            "contact_s": 60,
            "dissociation_s": 120,
            "flow_uL_per_min": 30,
            "cycles": "multi-cycle"
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "fitting": {
          "value": "Double reference and solvent correction; 1:1 kinetic fitting or steady-state Langmuir fitting; Insight Evaluation Software 4.0. Exact fit choice is not separately assigned to each table row.",
          "status": "verified",
          "source_id": "SV05B",
          "locator": "SPR method, PDF page 9",
          "note": ""
        },
        "negative_observation": {
          "value": {
            "label": "No binding",
            "numeric_threshold": null
          },
          "status": "verified",
          "source_id": "SV05A",
          "locator": "Table 1 NSD3 column",
          "note": "Qualitative source wording. No numerical threshold is inferred."
        },
        "identity_assignment": {
          "value": {
            "level": "source-labelled separated fraction",
            "isomer_label": 2,
            "retention_time_min": 8.2,
            "reported_diastereomeric_ratio": "97.10:2.90",
            "absolute_sulfolane_configuration": null,
            "exact_ccd_id": null
          },
          "status": "verified",
          "source_id": "SV05B",
          "locator": "Compounds 12/13, PDF page 14",
          "note": "Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment."
        }
      },
      "verified_updates": {
        "temperature_C": 25,
        "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
        "pH": 7.5,
        "DMSO_percent": 0.3,
        "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
        "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
        "conditions": {
          "protein_construct": "NSD3 263–398",
          "phase3_verification": "SV05A/SV05B",
          "purified_tags": "N-terminal 6His-TEV retained",
          "assay_substitutions": "not listed for assay constructs; 9FOE crystallographic mutants kept separate"
        },
        "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected"
      }
    }
  ],
  "preparations": [
    {
      "preparation_id": "PREP:COLLIE2025_11",
      "source_id": "SV05B",
      "locator": "PDF page 14",
      "verified_updates": {
        "identity_note": "Assayed diastereomeric preparation: pyrrolidine 2S fixed and sulfolane center racemic in the SI name. A1IEG/A1IFS are modeled candidates in 9FOC; mixture affinity is not assigned to either pure stereoisomer. Composition ratio and fraction-to-CCD assignment remain unresolved."
      },
      "affinity_transfer_allowed": false
    },
    {
      "preparation_id": "PREP:COLLIE2025_12",
      "source_id": "SV05B",
      "locator": "PDF page 14",
      "verified_updates": {
        "identity_note": "Separated compound 11 fraction labelled isomer 1; retention time 7.1 min, reported dr 98.84:1.16. Absolute sulfolane assignment and exact CCD mapping remain unresolved; no affinity transfer from compound 11."
      },
      "affinity_transfer_allowed": false
    },
    {
      "preparation_id": "PREP:COLLIE2025_13",
      "source_id": "SV05B",
      "locator": "PDF page 14",
      "verified_updates": {
        "identity_note": "Separated compound 11 fraction labelled isomer 2; retention time 8.2 min, reported dr 97.10:2.90. Absolute sulfolane assignment and exact CCD mapping remain unresolved; no affinity transfer from compound 11."
      },
      "affinity_transfer_allowed": false
    }
  ],
  "untargeted_assay_record_ids": [
    "PWWP:ASSAY:0001",
    "PWWP:ASSAY:0002",
    "PWWP:ASSAY:0010",
    "PWWP:ASSAY:0011",
    "PWWP:ASSAY:0012",
    "PWWP:ASSAY:0013",
    "PWWP:ASSAY:0032",
    "PWWP:ASSAY:0033",
    "PWWP:ASSAY:0034",
    "PWWP:ASSAY:0035",
    "PWWP:ASSAY:0036",
    "PWWP11:ASSAY:0008",
    "PWWP11:ASSAY:0009",
    "PWWP11:ASSAY:0010"
  ],
  "human_scientific_approval": false,
  "last_source_access_update_date": "2026-10-26",
  "public_projection_note": "Ephemeral retrieval-tool reference IDs and internal approval item lists omitted; original source URLs, scopes, source hashes, field values and status definitions preserved.",
  "source_sidecar_sha256": "5eed241482293cffb5f7f34363fb1752475c48e25d2d89486920e067018f028b"
}