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        ],
        "cellular_activity": false,
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        "entity_kind": "experimental_preparation",
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        "measured_observation_count": 0,
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        "other_pdb_component": false,
        "other_site_count": 0,
        "positive_direct_binder": false,
        "primary_view": "unresolved",
        "protein_degradation": false,
        "reported_control": false,
        "reported_probe": false,
        "rule": "Independent source observations + per-site coordinate role, not universal pharmacology",
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          "PWWP11:ASSAY:0009",
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        ],
        "source_site_ids": [],
        "structural_research_ligand": false,
        "structural_research_site_count": 0,
        "studied_compound": false,
        "total_observation_count": 3,
        "uncertain_site_count": 0,
        "uncertainty": "Classification is evidence- and experiment-scoped; missing assays do not prove nonbinding.",
        "verification_queue": true,
        "verification_status": "source_scoped_mixed"
      },
      "science_site_roles": {},
      "science_assay_roles": {
        "PWWP:ASSAY:0032": {
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          "rule": "Phase5 source-curated evidence_class, material, target_scope, source role; no inferred affinities",
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        },
        "PWWP11:ASSAY:0009": {
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          "compound_id": "PREP:NbH10",
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          "source_role": "macromolecular_binder",
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          "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified",
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          "value": 81.2,
          "verification_status": "source_scope_unresolved"
        }
      },
      "example": {
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        "domain_id": "UP:O75475:PWWP1",
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        "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/",
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        "is_measured": true,
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        "normalized_exact_uM": 0.09440000000000001,
        "numeric_transcription_status": "matches_primary_table",
        "numeric_value_inferred_from_structure": null,
        "organism": "Homo sapiens",
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        "pdb_id": null,
        "preparation_id": "PREP:NbH10",
        "protein_id": "UP:O75475",
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        "role": "macromolecular_binder",
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        "source_id": "S06",
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          "domain": "PWWP1",
          "domain_id": "UP:O75475:PWWP1",
          "endpoint_label_status": null,
          "evidence_class": "direct_binding_affinity",
          "evidence_location": "Table 1 NbH10 column; SPR methods",
          "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/",
          "gene": "PSIP1",
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          "molecular_entity_type": "nanobody",
          "numeric_transcription_status": "matches_primary_table",
          "numeric_value_inferred_from_structure": null,
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          "preparation_id": "PREP:NbH10",
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          "role": "macromolecular_binder",
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          "source_verification_scope": "local_primary_full_text_XML_table_and_methods_verified",
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          "uncertainty": 9.1,
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          "value_operator": "=",
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          "verification_level": null
        },
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        "source_reported_units": null,
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        "target_scope": "domain_specific",
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        {
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          "audit_decision": "retain_with_explicit_endpoint_and_identity",
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          "compound_name": "NbH10",
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            "buffer": "25 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM MgCl2; 1 mM DTT; 0.1% Tween 20; 0.1% BSA",
            "nucleosome_concentration_nM": 5,
            "preincubation": "30 minutes at 4 °C; after nucleosome addition 1 hour at 4 °C; after beads 1 hour at room temperature",
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          },
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                "buffer": "25 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM MgCl2; 1 mM DTT; 0.1% Tween 20; 0.1% BSA",
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                "preincubation": "30 minutes at 4 °C; after nucleosome addition 1 hour at 4 °C; after beads 1 hour at room temperature",
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              },
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            },
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            "source_id": "P5:S:VANTIEGHEM2026",
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            "domain_id": "UP:O75475:PWWP1",
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          "curation_version": "frozen-v1.2",
          "domain": "PWWP1",
          "domain_id": "UP:O75475:PWWP1",
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          "evidence_class": "direct_binding_affinity",
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          "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/",
          "gene": "PSIP1",
          "interpretation": "Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody–PWWP complex.",
          "is_measured": true,
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          "method": "SPR apparent",
          "molecular_entity_type": "nanobody",
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          "numeric_transcription_status": "matches_primary_table",
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          "preparation_id": "PREP:NbH10",
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          "role": "macromolecular_binder",
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            "chemical_identity_status": "named_macromolecule_no_full_sequence_identity",
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            "domain": "PWWP1",
            "domain_id": "UP:O75475:PWWP1",
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            "evidence_class": "direct_binding_affinity",
            "evidence_location": "Table 1 NbH10 column; SPR methods",
            "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/",
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            "molecular_entity_type": "nanobody",
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}