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      "context_pdb_count": 0,
      "context_negative_count": 1,
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      "context_bioactivity_only": true,
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        "other_component": false,
        "component_site_count": 0,
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            "assay_construct_start": 211,
            "assay_record_id": "PWWP:ASSAY:0022",
            "assay_type": "SPR Kd",
            "assay_value_transferred_to_modeled_stereoisomers": 0,
            "audit_decision": "retain_with_explicit_endpoint_and_identity",
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            "buffer": "10 mM HEPES; 150 mM NaCl; 3 mM EDTA; 0.1 mM TCEP; 0.05% (w/v) Tween-20; 0.3% (v/v) DMSO",
            "ccd_id": null,
            "ccd_mapping_source": null,
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            "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity",
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            "domain": "PWWP1",
            "domain_id": "UP:O96028:PWWP1",
            "endpoint_label_status": null,
            "evidence_class": "direct_binding_affinity",
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            "interpretation": "Affinity pertains to assay construct; crystal mutations and boundaries are separately recorded.",
            "molecular_entity_type": "small_molecule_preparation",
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            "protein_id": "UP:O96028",
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            "reference_id": "PMID:40959233",
            "remaining_uncertainty": "Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.",
            "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
            "role": "experimentally_studied_ligand",
            "source_component_url": null,
            "source_id": "S05",
            "source_reported_uncertainty": "0.03",
            "source_reported_units": "µM",
            "source_reported_value": "0.92",
            "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected",
            "temperature_C": 25,
            "uncertainty": 0.03,
            "uncertainty_type": "SD",
            "uncertainty_uM": 0.03,
            "unit_conversion": null,
            "units": "µM",
            "value": 0.92,
            "value_operator": "=",
            "value_uM": 0.92,
            "verification_level": "primary_full_text_sections_verified"
          },
          "source_reported_uncertainty": "0.03",
          "source_reported_units": "µM",
          "source_reported_value": "0.92",
          "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected",
          "target_scope": "domain_specific",
          "temperature_C": 25,
          "uncertainty": 0.03,
          "uncertainty_type": "SD",
          "uncertainty_uM": 0.03,
          "unit_conversion": null,
          "units": "µM",
          "value": 0.92,
          "value_operator": "=",
          "value_uM": 0.92,
          "verification_level": "primary_full_text_sections_verified"
        },
        {
          "DMSO_percent": 0.3,
          "abstract_reported_endpoint": null,
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          "assay_construct_start": 263,
          "assay_record_id": "PWWP11:ASSAY:0002",
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          "ccd_id": null,
          "ccd_mapping_source": null,
          "chemical_id": null,
          "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity",
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          "compound_name": "compound 5 (Collie 2025)",
          "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD3 263–398\", \"purified_tags\": \"N-terminal 6His-TEV retained\"}",
          "crystallographic_component_ids": null,
          "curation_version": "frozen-v1.2",
          "domain": "PWWP1",
          "domain_id": "UP:Q9BZ95:PWWP1",
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          "endpoint_label_status": null,
          "evidence_class": "censored_binding_limit",
          "evidence_location": "Table 1 NSD3 (263–398) column",
          "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/",
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          "interpretation": "Single counter-target experiment; not evidence of proteome-wide selectivity. No-binding rows have no inferred numerical threshold.",
          "is_measured": true,
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          "method": "SPR",
          "molecular_entity_type": "small_molecule_preparation",
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          "numeric_transcription_status": "matches_primary_table",
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          "organism": "Homo sapiens",
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          "pdb_id": null,
          "preparation_id": "PREP:COLLIE2025_5",
          "protein_id": "UP:Q9BZ95",
          "publication_year": 2025,
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          "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
          "role": "counter_target_assay",
          "source_component_url": null,
          "source_database": "Primary literature (frozen Atlas)",
          "source_id": "S05",
          "source_measurement_key": "5ae7180872e308784d9f0405d019dfa7b802c7cbaa846b6204ee6a8787a1437c",
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            "audit_decision": null,
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            "ccd_id": null,
            "ccd_mapping_source": null,
            "chemical_id": null,
            "chemical_identity_status": "assayed_stereoisomeric_mixture_not_single_chemical_identity",
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            "conditions": "{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD3 263–398\", \"purified_tags\": \"N-terminal 6His-TEV retained\"}",
            "crystallographic_component_ids": null,
            "domain": "PWWP1",
            "domain_id": "UP:Q9BZ95:PWWP1",
            "endpoint_label_status": null,
            "evidence_class": "censored_binding_limit",
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            "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/",
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            "molecular_entity_type": "small_molecule_preparation",
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            "replicates": "at least 3 independent replicates (Table 1 general SPR definition)",
            "role": "counter_target_assay",
            "source_component_url": null,
            "source_id": "S05",
            "source_reported_uncertainty": null,
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            "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected",
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            "uncertainty_uM": null,
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            "verification_level": null
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          "source_verification_scope": "Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected",
          "target_scope": "domain_specific",
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          "verification_level": null
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      "matched_assay_count": 3
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  ]
}