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No PWWP-domain affinity inferred; replicate/uncertainty/duration not populated without assay-specific verification.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:LIU2024", "source_reported_uncertainty": null, "source_reported_units": "percent", "source_reported_value": "96", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "percent", "value": 96, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }, "source_reported_uncertainty": null, "source_reported_units": "percent", "source_reported_value": "96", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "target_scope": "protein_level", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "percent", "value": 96, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" } ], "context_structures": [], "context_assay_count": 2, "context_measured_count": 2, "context_bound_count": 0, "context_pdb_count": 0, "context_negative_count": 0, "context_is_probe": false, "context_bioactivity_only": true, "context_kind": "Bioactivity only", "science": { "studied_compound": true, "measured_count": 2, "structural_research_ligand": false, "research_pdb_count": 0, "other_component": false, "component_site_count": 0, "verification_queue": false, "positive_direct_binder": false, "reported_probe": false, "reported_control": false, "cellular_activity": false, "protein_degradation": true, "bioactivity_only": true, "research_site_count": 0, "unknown_site_count": 0, "annotation_roles": [ "studied" ] }, "science_label": "Studied ยท no deposited PWWP research ligand", "science_profile": { "annotation_roles": [ "studied" ], "cellular_activity": false, "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "entity_kind": "chemical_identity", "identity_resolved": true, "measured_observation_count": 2, "name": "LLC0424", "other_pdb_component": false, "other_site_count": 0, "positive_direct_binder": false, "primary_view": "studied", "protein_degradation": true, "reported_control": false, "reported_probe": false, "rule": "Independent source observations + per-site coordinate role, not universal pharmacology", "source_observation_ids": [ "PWWP5:ASSAY:0025", "PWWP5:ASSAY:0026" ], "source_site_ids": [], "structural_research_ligand": false, "structural_research_site_count": 0, "studied_compound": true, "total_observation_count": 2, "uncertain_site_count": 0, "uncertainty": "Classification is evidence- and experiment-scoped; missing assays do not prove nonbinding.", "verification_queue": false, "verification_status": "source_scoped_mixed" }, "science_site_roles": {}, "science_assay_roles": { "PWWP5:ASSAY:0025": { "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "domain_id": null, "endpoint": "DC50", "evidence_type": "protein_degradation", "explicitly_negative": false, "measured": true, "method": "Immunoblot degradation", "molecular_entity_type": "small_molecule_preparation", "observation_id": "PWWP5:ASSAY:0025", "operator": "=", "positive_direct_binder": false, "preparation_id": "PREP:LLC0424", "protein_id": "UP:O96028", "remaining_uncertainty": "Cellular whole-protein measurement. PWWP1-directed design is supported by the source, but no direct affinity is inferred and this result is not assigned to a PWWP domain.", "reported_cellular": true, "reported_control": false, "reported_probe": false, "rule": "Phase5 source-curated evidence_class, material, target_scope, source role; no inferred affinities", "source_id": "P5:S:LIU2024", "source_location": "Abstract and Results", "source_role": "reported_degrader", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11094793/", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "studied_compound": true, "target_scope": "protein_level", "target_variant": null, "units": "nM", "value": 20, "verification_status": "primary_source_verified" }, "PWWP5:ASSAY:0026": { "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "domain_id": null, "endpoint": "Dmax", "evidence_type": "protein_degradation", "explicitly_negative": false, "measured": true, "method": "Immunoblot degradation", "molecular_entity_type": "small_molecule_preparation", "observation_id": "PWWP5:ASSAY:0026", "operator": "=", "positive_direct_binder": false, "preparation_id": "PREP:LLC0424", "protein_id": "UP:O96028", "remaining_uncertainty": "Cellular whole-protein measurement. No PWWP-domain affinity inferred; replicate/uncertainty/duration not populated without assay-specific verification.", "reported_cellular": true, "reported_control": false, "reported_probe": false, "rule": "Phase5 source-curated evidence_class, material, target_scope, source role; no inferred affinities", "source_id": "P5:S:LIU2024", "source_location": "Abstract and Results", "source_role": "reported_degrader", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11094793/", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "studied_compound": true, "target_scope": "protein_level", "target_variant": null, "units": "percent", "value": 96, "verification_status": "primary_source_verified" } }, "example": { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0025", "assay_type": "Immunoblot degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_name": "LLC0424", "conditions": { "cell_line": "RPMI-8402", "readout": "NSD2 protein" }, "curation_version": "phase5-1.0.0", "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Abstract and Results", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11094793/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_measured": true, "is_negative": false, "method": "Immunoblot degradation", "normalized_exact_uM": 0.02, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "organism": "Homo sapiens", "pdb_id": null, "preparation_id": "PREP:LLC0424", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38687638", "related_domain_id": "UP:O96028:PWWP1", "remaining_uncertainty": "Cellular whole-protein measurement. PWWP1-directed design is supported by the source, but no direct affinity is inferred and this result is not assigned to a PWWP domain.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_database": "Primary literature (Phase 5)", "source_id": "P5:S:LIU2024", "source_measurement_key": "3b5972b8e01515d09aef80be0d5b06ca8957d7c4f4b8fc0bcceae04bade7911a", "source_original": { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0025", "assay_type": "Immunoblot degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_name": "LLC0424", "conditions": { "cell_line": "RPMI-8402", "readout": "NSD2 protein" }, "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Abstract and Results", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11094793/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:LLC0424", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38687638", "related_domain_id": "UP:O96028:PWWP1", "remaining_uncertainty": "Cellular whole-protein measurement. PWWP1-directed design is supported by the source, but no direct affinity is inferred and this result is not assigned to a PWWP domain.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:LIU2024", "source_reported_uncertainty": null, "source_reported_units": "nM", "source_reported_value": "20", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "nM", "value": 20, "value_operator": "=", "value_uM": 0.02, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }, "source_reported_uncertainty": null, "source_reported_units": "nM", "source_reported_value": "20", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "target_scope": "protein_level", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "nM", "value": 20, "value_operator": "=", "value_uM": 0.02, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }, "matched_observations": [ { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0025", "assay_type": "Immunoblot degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_name": "LLC0424", "conditions": { "cell_line": "RPMI-8402", "readout": "NSD2 protein" }, "curation_version": "phase5-1.0.0", "domain": null, "domain_id": null, "endpoint": "DC50", "evidence_class": "cellular_protein_degradation", "evidence_location": "Abstract and Results", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11094793/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_measured": true, "is_negative": false, "method": "Immunoblot degradation", "normalized_exact_uM": 0.02, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "organism": "Homo sapiens", "pdb_id": null, "preparation_id": "PREP:LLC0424", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38687638", "related_domain_id": "UP:O96028:PWWP1", "remaining_uncertainty": "Cellular whole-protein measurement. 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PWWP1-directed design is supported by the source, but no direct affinity is inferred and this result is not assigned to a PWWP domain.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:LIU2024", "source_reported_uncertainty": null, "source_reported_units": "nM", "source_reported_value": "20", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "nM", "value": 20, "value_operator": "=", "value_uM": 0.02, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }, "source_reported_uncertainty": null, "source_reported_units": "nM", "source_reported_value": "20", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "target_scope": "protein_level", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "nM", "value": 20, "value_operator": "=", "value_uM": 0.02, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }, { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0026", "assay_type": "Immunoblot degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_name": "LLC0424", "conditions": { "cell_line": "RPMI-8402", "readout": "NSD2 protein" }, "curation_version": "phase5-1.0.0", "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Abstract and Results", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11094793/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_measured": true, "is_negative": false, "method": "Immunoblot degradation", "normalized_exact_uM": null, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "organism": "Homo sapiens", "pdb_id": null, "preparation_id": "PREP:LLC0424", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38687638", "related_domain_id": "UP:O96028:PWWP1", "remaining_uncertainty": "Cellular whole-protein measurement. No PWWP-domain affinity inferred; replicate/uncertainty/duration not populated without assay-specific verification.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_database": "Primary literature (Phase 5)", "source_id": "P5:S:LIU2024", "source_measurement_key": "957f196e31ed8ef11b42859ddd36da15402952187b7ad34679776f8f3335e69e", "source_original": { "assay_construct_end": null, "assay_construct_start": null, "assay_context": "cellular_full_protein", "assay_organism": "Homo sapiens", "assay_record_id": "PWWP5:ASSAY:0026", "assay_type": "Immunoblot degradation", "assay_value_transferred_to_modeled_stereoisomers": false, "ccd_id": null, "chemical_id": "INCHIKEY:OHRABUZLZFHHEJ-UHFFFAOYSA-N", "compound_name": "LLC0424", "conditions": { "cell_line": "RPMI-8402", "readout": "NSD2 protein" }, "domain": null, "domain_id": null, "endpoint": "Dmax", "evidence_class": "cellular_protein_degradation", "evidence_location": "Abstract and Results", "evidence_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11094793/", "gene": "NSD2", "inclusion_rationale": "Primary source explicitly identifies this preparation, target context and observation.", "is_negative": false, "numeric_transcription_status": "source_preserved", "numeric_value_inferred_from_structure": false, "pdb_id": null, "preparation_id": "PREP:LLC0424", "protein_id": "UP:O96028", "publication_year": 2024, "reference_id": "PMID:38687638", "related_domain_id": "UP:O96028:PWWP1", "remaining_uncertainty": "Cellular whole-protein measurement. No PWWP-domain affinity inferred; replicate/uncertainty/duration not populated without assay-specific verification.", "replicates": null, "retrieval_date": "2026-10-26", "role": "reported_degrader", "source_id": "P5:S:LIU2024", "source_reported_uncertainty": null, "source_reported_units": "percent", "source_reported_value": "96", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "percent", "value": 96, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" }, "source_reported_uncertainty": null, "source_reported_units": "percent", "source_reported_value": "96", "source_verification_scope": "NCBI BioC full text; abstract, Results, chemical synthesis names and figure captions inspected", "target_scope": "protein_level", "uncertainty": null, "uncertainty_type": null, "uncertainty_uM": null, "units": "percent", "value": 96, "value_operator": "=", "value_uM": null, "verification_level": "primary_source_verified", "version": "phase5.chemical_curation.1.1.0" } ], "matched_assay_count": 2 } ] }