{"count":4,"filters":{"construct":"","domain":"","endpoint":"","evidence_type":"","max_um":"","method":"","min_um":"","missing":"","protein":"UP:O96028","q":"","result":"","scope":"","source":"","structure":"","variant":"","verification":"","year":""},"observations":[{"chemical_identity":null,"compound_name":"UNC10088","conditions":"Without FBXO22 FIST domain","domain_id":"UP:O96028:PWWP1","endpoint":"IC50","evidence_type":"biochemical_competition","has_unmodified_binary_pwwp_structure":false,"identity_status":"published_name_only_exact_assayed_identity_unresolved","limitation":"The prepared chemical parent is not stereochemistry/batch resolved; IC50 not Kd. Do not count FBXO22-conditional results as independent chemical identities.","method":"TR-FRET competitive displacement of biotinylated UNC7096","observation_id":"P6B:ASSAY:0001","positive_binding_interpretation":"positive competitive engagement; not an equilibrium Kd","preparation_id":"P6B:PREP:UNC10088","protein_id":"UP:O96028","relation":"=","replicates":"n=3 independent experiments","source_doi":"10.1038/s41467-026-72235-9","source_id":"P6B:S:AMANN2026","source_location":"Figure 2h and Supplementary Figure 3D","source_title":"Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22","source_url":"https://www.nature.com/articles/s41467-026-72235-9","structure_links":[{"component":"UNC10088-C derivative; not mapped to exact unmodified administered molecule","coordinate_evidence":"RCSB deposited ligand listing and entry metadata; author-reported ternary interface","deposited_ccd":"A1J20","fbxo22_chain":"A","interpretation":"Not a binary NSD2 PWWP1 ligand pose for the parent preparation","ligand_auth_asym_id":"B","ligand_label_asym_id":"F","method":"ELECTRON MICROSCOPY","pdb_id":"29HG","preparation_id":"P6B:PREP:UNC10088","rcsb_url":"https://www.rcsb.org/structure/29HG","resolution_angstrom":4.0,"source_id":"P6B:S:AMANN2026","source_inspected_location":"RCSB PDB 29HG Experimental Data Snapshot; Small Molecules; Macromolecules","structural_verification_scope":"RCSB entry metadata and deposited component/chain listing independently checked; map density not independently inspected","structure_relationship":"chemical_transformation_product_in_FBXO22_NSD2_PWWP1_ternary_complex","target_chain":"B","verified_status":"source_reported_ternary_deposited"}],"target_scope":"domain_specific","tested_construct":"His-tagged NSD2 PWWP1; exact sequence boundaries not independently extracted","uncertainty":22,"uncertainty_type":"SEM","units":"nM","value":248,"value_text":"=248 \u00b1 22 nM","variant":"wild type not separately certified","verification_status":"accepted_with_bounded_interpretation"},{"chemical_identity":null,"compound_name":"UNC10088","conditions":"With wild-type FBXO22 FIST domain; source-reported cooperative factor alpha = 3.3, not 248/101","domain_id":"UP:O96028:PWWP1","endpoint":"IC50","evidence_type":"biochemical_competition","has_unmodified_binary_pwwp_structure":false,"identity_status":"published_name_only_exact_assayed_identity_unresolved","limitation":"Two different experimental contexts for one preparation; do not average or replace one with another.","method":"TR-FRET competitive displacement of biotinylated UNC7096","observation_id":"P6B:ASSAY:0002","positive_binding_interpretation":"positive cooperative engagement in a ternary context; not an equilibrium Kd","preparation_id":"P6B:PREP:UNC10088","protein_id":"UP:O96028","relation":"=","replicates":"n=3 independent experiments","source_doi":"10.1038/s41467-026-72235-9","source_id":"P6B:S:AMANN2026","source_location":"Figure 2h, Supplementary Figure 3D and Results","source_title":"Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22","source_url":"https://www.nature.com/articles/s41467-026-72235-9","structure_links":[{"component":"UNC10088-C derivative; not mapped to exact unmodified administered molecule","coordinate_evidence":"RCSB deposited ligand listing and entry metadata; author-reported ternary interface","deposited_ccd":"A1J20","fbxo22_chain":"A","interpretation":"Not a binary NSD2 PWWP1 ligand pose for the parent preparation","ligand_auth_asym_id":"B","ligand_label_asym_id":"F","method":"ELECTRON MICROSCOPY","pdb_id":"29HG","preparation_id":"P6B:PREP:UNC10088","rcsb_url":"https://www.rcsb.org/structure/29HG","resolution_angstrom":4.0,"source_id":"P6B:S:AMANN2026","source_inspected_location":"RCSB PDB 29HG Experimental Data Snapshot; Small Molecules; Macromolecules","structural_verification_scope":"RCSB entry metadata and deposited component/chain listing independently checked; map density not independently inspected","structure_relationship":"chemical_transformation_product_in_FBXO22_NSD2_PWWP1_ternary_complex","target_chain":"B","verified_status":"source_reported_ternary_deposited"}],"target_scope":"domain_specific","tested_construct":"His-tagged NSD2 PWWP1 + FBXO22 FIST; exact sequence boundaries not independently extracted","uncertainty":8,"uncertainty_type":"SEM","units":"nM","value":101,"value_text":"=101 \u00b1 8 nM","variant":"wild-type FBXO22 FIST; NSD2 variant not specified","verification_status":"accepted_with_bounded_interpretation"},{"chemical_identity":null,"compound_name":"UNC10415667","conditions":"U2OS NSD2 HiBiT dose response","domain_id":null,"endpoint":"DC50","evidence_type":"protein_degradation","has_unmodified_binary_pwwp_structure":false,"identity_status":"published_name_only_exact_assayed_identity_unresolved","limitation":"Do not copy this whole-protein DC50 to NSD2 PWWP1. 75% at 1 \u00b5M for 6 hours is a separate endpoint, not the same dose-response value.","method":"Cellular HiBiT protein-degradation dose response","observation_id":"P6B:ASSAY:0003","positive_binding_interpretation":"cellular whole-protein depletion; no isolated-domain affinity","preparation_id":"P6B:PREP:UNC10415667","protein_id":"UP:O96028","relation":"=","replicates":"n=2 independent experiments (final 2026 article)","source_doi":"10.1038/s41467-026-72235-9","source_id":"P6B:S:AMANN2026","source_location":"Figure 3e legend and Results","source_title":"Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22","source_url":"https://www.nature.com/articles/s41467-026-72235-9","structure_links":[{"component":"UNC10415667-C derivative","coordinate_evidence":"RCSB deposited ligand listing and entry metadata; author-reported ternary interface","deposited_ccd":"A1J21","fbxo22_chain":"A","interpretation":"Not an experimentally verified binary ligand pose for the unmodified compound","ligand_auth_asym_id":"A","ligand_label_asym_id":"F","method":"ELECTRON MICROSCOPY","pdb_id":"29HI","preparation_id":"P6B:PREP:UNC10415667","rcsb_url":"https://www.rcsb.org/structure/29HI","resolution_angstrom":5.9,"source_id":"P6B:S:AMANN2026","source_inspected_location":"RCSB PDB 29HI Experimental Data Snapshot; Small Molecules; Macromolecules","structural_verification_scope":"RCSB entry metadata and deposited component/chain listing independently checked; map density not independently inspected","structure_relationship":"chemical_transformation_product_in_FBXO22_NSD2_PWWP1_ternary_complex","target_chain":"B","verified_status":"source_reported_ternary_deposited"}],"target_scope":"protein_level_cellular","tested_construct":"U2OS NSD2-HiBiT cells","uncertainty":null,"uncertainty_type":"figure caption reports SEM but numeric uncertainty was not independently extracted","units":"nM","value":460,"value_text":"=460 nM","variant":"not stated","verification_status":"accepted_with_bounded_interpretation"},{"chemical_identity":null,"compound_name":"UNC12149","conditions":"U2OS NSD2 HiBiT dose response","domain_id":null,"endpoint":"DC50","evidence_type":"protein_degradation","has_unmodified_binary_pwwp_structure":false,"identity_status":"published_name_only_exact_assayed_identity_unresolved","limitation":"Do not transfer cellular DC50 to a particular PWWP-domain biochemical binding constant.","method":"Cellular HiBiT protein-degradation dose response","observation_id":"P6B:ASSAY:0004","positive_binding_interpretation":"cellular whole-protein depletion; no isolated-domain affinity","preparation_id":"P6B:PREP:UNC12149","protein_id":"UP:O96028","relation":"=","replicates":"n=2 independent experiments (final 2026 article)","source_doi":"10.1038/s41467-026-72235-9","source_id":"P6B:S:AMANN2026","source_location":"Figure 4h legend and Results","source_title":"Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22","source_url":"https://www.nature.com/articles/s41467-026-72235-9","structure_links":[],"target_scope":"protein_level_cellular","tested_construct":"U2OS NSD2-HiBiT cells","uncertainty":null,"uncertainty_type":"figure caption reports SEM but numeric uncertainty was not independently extracted","units":"nM","value":120,"value_text":"=120 nM","variant":"not stated","verification_status":"accepted_with_bounded_interpretation"}],"preparation_count":3,"version":"phase6b-1.0.0"}