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No-binding rows have no inferred numerical threshold.","molecular_entity_type":"small_molecule_preparation","numeric_transcription_status":"matches_primary_table","numeric_value_inferred_from_structure":null,"pH":7.5,"pdb_id":null,"preparation_id":"PREP:COLLIE2025_7","protein_id":"UP:Q9BZ95","qualitative_result":null,"reference_id":"PMID:40959233","remaining_uncertainty":"Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.","replicates":"at least 3 independent replicates (Table 1 general SPR definition)","role":"counter_target_assay","source_component_url":null,"source_id":"S05","source_reported_uncertainty":null,"source_reported_units":null,"source_reported_value":null,"source_verification_scope":"Phase 3 primary Table 1 and actual supporting PDF pages 7, 9 and 14; the inherited HTRF method remains explicitly not inspected","temperature_C":25,"uncertainty":null,"uncertainty_type":null,"uncertainty_uM":null,"unit_conversion":null,"units":"\u00b5M","value":30.0,"value_operator":">","value_uM":30.0,"verification_level":null}],"interpretation":"Exact links require chemical identity and the same native target/domain. An assay value remains attached to its measured construct and preparation. Unresolved and negative observations remain available without an exact-contact link.","protein":{"aliases":"[\"Nuclear SET domain-containing protein 3\",\"Protein whistle\",\"WHSC1-like 1 isoform 9 with methyltransferase activity to lysine\",\"WHSC1-like protein 1\",\"WHSC1L1\",\"Wolf-Hirschhorn syndrome candidate 1-like protein 1\"]","attribution":"UniProt, CC BY 4.0","gene":"NSD3","length":1437,"membership_tier":"annotation_supported","name":"Histone-lysine N-methyltransferase NSD3","organism":"Homo sapiens","protein_existence_caution":null,"protein_id":"UP:Q9BZ95","sequence":"MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGASEISDSCKPLKKRSRASTDVEMTSSAYRDTSDSDSRGLSDLQVGFGKQVDSPSATADADVSDVQSMDSSLSRRGTGMSKKDTVCQICESSGDSLIPCEGECCKHFHLECLGLASLPDSKFICMECKTGQHPCFSCKVSGKDVKRCSVGACGKFYHEACVRKFPTAIFESKGFRCPQHCCSACSMEKDIHKASKGRMMRCLRCPVAYHSGDACIAAGSMLVSSYILICSNHSKRSSNSSAVNVGFCFVCARGLIVQDHSDPMFSSYAYKSHYLLNESNRAELMKLPMIPSSSASKKKCEKGGRLLCCESCPASFHPECLSIEMPEGCWNCNDCKAGKKLHYKQIVWVKLGNYRWWPAEICNPRSVPLNIQGLKHDLGDFPVFFFGSHDYYWVHQGRVFPYVEGDKSFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKVIGKVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCPAGDRCQNQCFTKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIPAGMELTFNYNLDCLGNGRTECHCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRKIKTEPKQMHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPYGKWECPWHQCDECSSAAVSFCEFCPHSFCKDHEKGALVPSALEGRLCCSEHDPMAPVSPEYWSKIKCKWESQDHGEEVKE","sequence_sha256":"1740a00df4b91dc186680dfedb4b8b1eaed2f508c951cb44f0a8a5995c52ba63","source_url":"https://www.uniprot.org/uniprotkb/Q9BZ95","taxonomy_id":"9606","uniprot":"Q9BZ95"},"structure_contact_assay_links":[{"assay_construct_end":398,"assay_construct_start":263,"assay_evidence_class":"direct_binding_affinity","assay_record_id":"PWWP:ASSAY:0008","assay_type":"SPR Kd","ccd_id":"EJE","chain_id":"PDB:6G2O:CHAIN:A:UP:Q9BZ95","chemical_id":"INCHIKEY:WOAOENGFAAUUGT-UHFFFAOYSA-N","compound_name":"BI-9321","contact_class":"native_annotation_core_contact","contact_distance_rank":1,"domain_id":"UP:Q9BZ95:PWWP1","evidence_url":"https://www.thesgc.org/chemical-probes/bi-9321","ligand_instance_id":"PDB:6G2O:LIG:B:EJE:401:.","link_semantics":"same exact chemical identity and native domain; construct equivalence not assumed","minimum_Pfam_distance_angstrom":2.8197,"minimum_core_distance_angstrom":2.8197,"multiple_contacting_mapped_chains":0,"pdb_id":"6G2O","potency_transferred_to_crystal_construct":0,"preparation_id":"PREP:BI-9321","protein_id":"UP:Q9BZ95","protein_label_asym_id":"A","remaining_uncertainty":"Primary replicate conflict: Figure 3a states n=3 independent experiments, while Supplementary Table 7 states SPR n=4; both report SD. Indexed primary SPR Methods supply residues 263\u2013398 and buffer, but the full PDF was unavailable. Exact assay temperature, mutations and per-dose contact times remain not inspected.","site_id":"PDB:6G2O:LIG:B:EJE:401:.:SITE:UP:Q9BZ95:PWWP1:A","source_verification_scope":"primary_probe_developer_data_page; original journal full text not inspected","units":"nM","value":166.0,"value_operator":"="},{"assay_construct_end":null,"assay_construct_start":null,"assay_evidence_class":"cellular_interaction_response","assay_record_id":"PWWP:ASSAY:0009","assay_type":"NanoBRET IC50","ccd_id":"EJE","chain_id":"PDB:6G2O:CHAIN:A:UP:Q9BZ95","chemical_id":"INCHIKEY:WOAOENGFAAUUGT-UHFFFAOYSA-N","compound_name":"BI-9321","contact_class":"native_annotation_core_contact","contact_distance_rank":1,"domain_id":"UP:Q9BZ95:PWWP1","evidence_url":"https://www.thesgc.org/chemical-probes/bi-9321","ligand_instance_id":"PDB:6G2O:LIG:B:EJE:401:.","link_semantics":"same exact chemical identity and native domain; construct equivalence not assumed","minimum_Pfam_distance_angstrom":2.8197,"minimum_core_distance_angstrom":2.8197,"multiple_contacting_mapped_chains":0,"pdb_id":"6G2O","potency_transferred_to_crystal_construct":0,"preparation_id":"PREP:BI-9321","protein_id":"UP:Q9BZ95","protein_label_asym_id":"A","remaining_uncertainty":"The value 1.2 \u00b1 0.06 uM, SD and n=3 were verified in the actual Supplementary Table 7. Full NanoBRET construct and conditions were not inspected. The 1.4 uM direct tracer-engagement endpoint is separate from this histone PPI assay.","site_id":"PDB:6G2O:LIG:B:EJE:401:.:SITE:UP:Q9BZ95:PWWP1:A","source_verification_scope":"primary_probe_developer_data_page; original journal full text not inspected","units":"\u00b5M","value":1.2,"value_operator":"="}]}