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Crystallography 211\u2013350 is not assigned as the binding-assay construct.","site_id":"PDB:7MDN:LIG:R:Y6V:401:.:SITE:UP:O96028:PWWP1:G","source_verification_scope":"primary_article_selected_indexed_full_text_passages; original supplement not independently opened","units":"nM","value":349.0,"value_operator":"="},{"assay_construct_end":null,"assay_construct_start":null,"assay_evidence_class":"direct_binding_affinity","assay_record_id":"PWWP:ASSAY:0003","assay_type":"SPR Kd","ccd_id":"Y6V","chain_id":"PDB:7MDN:CHAIN:H:UP:O96028","chemical_id":"INCHIKEY:XPHOPAPMJBSLFN-UHFFFAOYSA-N","compound_name":"MRT866","contact_class":"native_annotation_core_contact","contact_distance_rank":1,"domain_id":"UP:O96028:PWWP1","evidence_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9189931/","ligand_instance_id":"PDB:7MDN:LIG:R:Y6V:401:.","link_semantics":"same exact chemical identity and native domain; construct equivalence not assumed","minimum_Pfam_distance_angstrom":2.8979,"minimum_core_distance_angstrom":2.8979,"multiple_contacting_mapped_chains":1,"pdb_id":"7MDN","potency_transferred_to_crystal_construct":0,"preparation_id":"PREP:MRT866","protein_id":"UP:O96028","protein_label_asym_id":"H","remaining_uncertainty":"349 \u00b1 19 nM verified in Results; MRT866-specific SPR construct/protocol, replicate definition and uncertainty statistic remain unresolved. Crystallography 211\u2013350 is not assigned as the binding-assay construct.","site_id":"PDB:7MDN:LIG:R:Y6V:401:.:SITE:UP:O96028:PWWP1:H","source_verification_scope":"primary_article_selected_indexed_full_text_passages; original supplement not independently opened","units":"nM","value":349.0,"value_operator":"="},{"assay_construct_end":368,"assay_construct_start":208,"assay_evidence_class":"direct_binding_affinity","assay_record_id":"PWWP:ASSAY:0017","assay_type":"SPR Kd","ccd_id":"A1IEF","chain_id":"PDB:9FOE:CHAIN:A:UP:O96028","chemical_id":"INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N","compound_name":"compound 7 (Collie 2025)","contact_class":"native_annotation_core_contact","contact_distance_rank":1,"domain_id":"UP:O96028:PWWP1","evidence_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","ligand_instance_id":"PDB:9FOE:LIG:B:A1IEF:401:.","link_semantics":"same exact chemical identity and native domain; construct equivalence not assumed","minimum_Pfam_distance_angstrom":3.1245,"minimum_core_distance_angstrom":3.1245,"multiple_contacting_mapped_chains":0,"pdb_id":"9FOE","potency_transferred_to_crystal_construct":0,"preparation_id":"PREP:COLLIE2025_7","protein_id":"UP:O96028","protein_label_asym_id":"A","remaining_uncertainty":"Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.","site_id":"PDB:9FOE:LIG:B:A1IEF:401:.:SITE:UP:O96028:PWWP1:A","source_verification_scope":"local_primary_full_text_XML; Table 1 and relevant Results inspected; supplement not inspected","units":"\u00b5M","value":0.15,"value_operator":"="},{"assay_construct_end":350,"assay_construct_start":211,"assay_evidence_class":"direct_binding_affinity","assay_record_id":"PWWP:ASSAY:0024","assay_type":"SPR Kd","ccd_id":"A1IEF","chain_id":"PDB:9FOE:CHAIN:A:UP:O96028","chemical_id":"INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N","compound_name":"compound 7 (Collie 2025)","contact_class":"native_annotation_core_contact","contact_distance_rank":1,"domain_id":"UP:O96028:PWWP1","evidence_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","ligand_instance_id":"PDB:9FOE:LIG:B:A1IEF:401:.","link_semantics":"same exact chemical identity and native domain; construct equivalence not assumed","minimum_Pfam_distance_angstrom":3.1245,"minimum_core_distance_angstrom":3.1245,"multiple_contacting_mapped_chains":0,"pdb_id":"9FOE","potency_transferred_to_crystal_construct":0,"preparation_id":"PREP:COLLIE2025_7","protein_id":"UP:O96028","protein_label_asym_id":"A","remaining_uncertainty":"Assay-specific buffer and temperature verified from SI page 9; tag/construct from Table S2. Exact per-row kinetic-versus-steady-state fit choice and exact n beyond table footnote not specified. Chemical preparation identity remains as curated.","site_id":"PDB:9FOE:LIG:B:A1IEF:401:.:SITE:UP:O96028:PWWP1:A","source_verification_scope":"local_primary_full_text_XML; Table 1 and relevant Results inspected; supplement not inspected","units":"\u00b5M","value":0.76,"value_operator":"="},{"assay_construct_end":368,"assay_construct_start":208,"assay_evidence_class":"biochemical_interaction_inhibition","assay_record_id":"PWWP:ASSAY:0029","assay_type":"HTRF displacement IC50","ccd_id":"A1IEF","chain_id":"PDB:9FOE:CHAIN:A:UP:O96028","chemical_id":"INCHIKEY:HHACEXLLNHJLFT-INIZCTEOSA-N","compound_name":"compound 7 (Collie 2025)","contact_class":"native_annotation_core_contact","contact_distance_rank":1,"domain_id":"UP:O96028:PWWP1","evidence_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","ligand_instance_id":"PDB:9FOE:LIG:B:A1IEF:401:.","link_semantics":"same exact chemical identity and native domain; construct equivalence not assumed","minimum_Pfam_distance_angstrom":3.1245,"minimum_core_distance_angstrom":3.1245,"multiple_contacting_mapped_chains":0,"pdb_id":"9FOE","potency_transferred_to_crystal_construct":0,"preparation_id":"PREP:COLLIE2025_7","protein_id":"UP:O96028","protein_label_asym_id":"A","remaining_uncertainty":"HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.","site_id":"PDB:9FOE:LIG:B:A1IEF:401:.:SITE:UP:O96028:PWWP1:A","source_verification_scope":"local_primary_full_text_XML; Table 1 and relevant Results inspected; supplement not inspected","units":"\u00b5M","value":0.21,"value_operator":"="}]}