{"assay_preparations":[{"chemical_id":"INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N","entity_type":"small_molecule_preparation","exact_ccd_id":"A1D73","identity_note":"Complete deposited identity retained; no connectivity-only or name-only merge.","identity_status":"exact_single_CCD_identity","modeled_candidate_ccd_ids":"[]","modeled_candidate_chemical_ids":"[]","name":"BPP","preparation_id":"PREP:BPP","role":"assay_preparation","sequence_if_defined":null},{"chemical_id":null,"entity_type":"nanobody","exact_ccd_id":null,"identity_note":"Complete deposited identity retained; no connectivity-only or name-only merge.","identity_status":"named_macromolecule_no_full_sequence_identity","modeled_candidate_ccd_ids":"[]","modeled_candidate_chemical_ids":"[]","name":"NbH10","preparation_id":"PREP:NbH10","role":"assay_preparation","sequence_if_defined":null}],"assays_without_exact_contact_link":[{"DMSO_percent":null,"abstract_reported_endpoint":null,"abstract_reported_method":null,"abstract_reported_units":null,"abstract_reported_value":null,"assay_construct_end":null,"assay_construct_start":null,"assay_record_id":"PWWP:ASSAY:0012","assay_type":"ITC Kd","assay_value_transferred_to_modeled_stereoisomers":0,"audit_decision":"retain_with_explicit_endpoint_and_identity","baseline_assay_record":1,"buffer":null,"ccd_id":"A1D73","ccd_mapping_source":"Provenance/Literature/ligand_ccd_associations.json","chemical_id":"INCHIKEY:LKKNQJJSEMQTHQ-UHFFFAOYSA-N","chemical_identity_status":"exact_single_CCD_identity","component_context":null,"compound_name":"BPP","conditions":"{}","crystallographic_component_ids":null,"domain":"PWWP","domain_id":"UP:O75475:PWWP1","endpoint_label_status":null,"evidence_class":"direct_binding_affinity","evidence_location":"Results; Figure 4B","evidence_url":"https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14981","gene":"PSIP1","interpretation":"Binding is established in solution; no PSIP1\u2013BPP crystal structure is assigned here.","molecular_entity_type":"small_molecule_preparation","numeric_transcription_status":"matches_primary_report","numeric_value_inferred_from_structure":0,"pH":null,"pdb_id":null,"preparation_id":"PREP:BPP","protein_id":"UP:O75475","qualitative_result":null,"reference_id":"PMID:39031937","remaining_uncertainty":"Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.","replicates":null,"role":"experimentally_studied_fragment_ligand","source_component_url":null,"source_id":"S04","source_reported_uncertainty":"2","source_reported_units":"\u00b5M","source_reported_value":"27","source_verification_scope":"primary_publisher_selected_indexed_Results_and_Methods_passages; Figure S2 not independently retrieved","temperature_C":null,"uncertainty":2.0,"uncertainty_type":"fitting_error","uncertainty_uM":2.0,"unit_conversion":null,"units":"\u00b5M","value":27.0,"value_operator":"=","value_uM":27.0,"verification_level":"primary_full_text_sections_verified"},{"DMSO_percent":null,"abstract_reported_endpoint":null,"abstract_reported_method":null,"abstract_reported_units":null,"abstract_reported_value":null,"assay_construct_end":90,"assay_construct_start":1,"assay_record_id":"PWWP11:ASSAY:0008","assay_type":"SPR apparent Kd","assay_value_transferred_to_modeled_stereoisomers":0,"audit_decision":null,"baseline_assay_record":0,"buffer":"50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20","ccd_id":null,"ccd_mapping_source":null,"chemical_id":null,"chemical_identity_status":"named_macromolecule_no_full_sequence_identity","component_context":null,"compound_name":"NbH10","conditions":"{\"protein_construct\": \"PSIP1 1\u201390\", \"replicates\": 3, \"uncertainty_type\": \"SD\"}","crystallographic_component_ids":null,"domain":"PWWP1","domain_id":"UP:O75475:PWWP1","endpoint_label_status":null,"evidence_class":"direct_binding_affinity","evidence_location":"Table 1 NbH10 column; SPR methods","evidence_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/","gene":"PSIP1","interpretation":"Apparent SPR affinity of a nanobody; not a small molecule or a solved nanobody\u2013PWWP complex.","molecular_entity_type":"nanobody","numeric_transcription_status":"matches_primary_table","numeric_value_inferred_from_structure":null,"pH":7.4,"pdb_id":null,"preparation_id":"PREP:NbH10","protein_id":"UP:O75475","qualitative_result":null,"reference_id":"PMID:42194065","remaining_uncertainty":null,"replicates":"3","role":"macromolecular_binder","source_component_url":null,"source_id":"S06","source_reported_uncertainty":null,"source_reported_units":null,"source_reported_value":null,"source_verification_scope":"local_primary_full_text_XML_table_and_methods_verified","temperature_C":25,"uncertainty":9.1,"uncertainty_type":"SD","uncertainty_uM":0.0091,"unit_conversion":null,"units":"nM","value":94.4,"value_operator":"=","value_uM":0.0944,"verification_level":null},{"DMSO_percent":null,"abstract_reported_endpoint":null,"abstract_reported_method":null,"abstract_reported_units":null,"abstract_reported_value":null,"assay_construct_end":null,"assay_construct_start":null,"assay_record_id":"PWWP:ASSAY:0032","assay_type":"AlphaScreen inhibition IC50","assay_value_transferred_to_modeled_stereoisomers":0,"audit_decision":"retain_with_explicit_endpoint_and_identity","baseline_assay_record":1,"buffer":null,"ccd_id":null,"ccd_mapping_source":null,"chemical_id":null,"chemical_identity_status":"named_macromolecule_no_full_sequence_identity","component_context":null,"compound_name":"NbH10","conditions":"{\"partner\": \"H3K36me3 nucleosomes\", \"protein\": \"full-length LEDGF/p75\"}","crystallographic_component_ids":null,"domain":"PWWP","domain_id":"UP:O75475:PWWP1","endpoint_label_status":null,"evidence_class":"biochemical_interaction_inhibition","evidence_location":"Section 3.6; Figure 3","evidence_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13204740/","gene":"PSIP1","interpretation":"Macromolecular binder. NbH10-alone crystal coordinates do not establish a PWWP-bound complex.","molecular_entity_type":"nanobody","numeric_transcription_status":"matches_primary_report","numeric_value_inferred_from_structure":0,"pH":null,"pdb_id":null,"preparation_id":"PREP:NbH10","protein_id":"UP:O75475","qualitative_result":null,"reference_id":"PMID:42194065","remaining_uncertainty":"Full assay buffer, temperature and incubation metadata not transcribed; null means uncurated, not absent.","replicates":null,"role":"experimentally_studied_macromolecular_binder","source_component_url":null,"source_id":"S06","source_reported_uncertainty":"0.15","source_reported_units":"\u00b5M","source_reported_value":"0.94","source_verification_scope":"local_primary_full_text_XML; Table 1, methods and Results inspected","temperature_C":null,"uncertainty":0.15,"uncertainty_type":null,"uncertainty_uM":0.15,"unit_conversion":null,"units":"\u00b5M","value":0.94,"value_operator":"=","value_uM":0.94,"verification_level":"primary_full_text_sections_verified"}],"interpretation":"Exact links require chemical identity and the same native target/domain. An assay value remains attached to its measured construct and preparation. Unresolved and negative observations remain available without an exact-contact link.","protein":{"aliases":"[\"CLL-associated antigen KW-7\",\"DFS 70\",\"DFS70\",\"Dense fine speckles 70 kDa protein\",\"LEDGF\",\"Lens epithelium-derived growth factor\",\"PSIP2\",\"Transcriptional coactivator p75/p52\"]","attribution":"UniProt, CC BY 4.0","gene":"PSIP1","length":530,"membership_tier":"annotation_supported","name":"PC4 and SFRS1-interacting protein","organism":"Homo sapiens","protein_existence_caution":null,"protein_id":"UP:O75475","sequence":"MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEQQNKDEGKKPEVKKVEKKRETSMDSRLQRIHAEIKNSLKIDNLDVNRCIEALDELASLQVTMQQAQKHTEMITTLKKIRRFKVSQVIMEKSTMLYNKFKNMFLVGEGDSVITQVLNKSLAEQRQHEEANKTKDQGKKGPNKKLEKEQTGSKTLNGGSDAQDGNQPQHNGESNEDSKDNHEASTKKKPSSEERETEISLKDSTLDN","sequence_sha256":"a0d8504fc2ce28870415fc343e3f9d05f8ab9b574fa9e28f0f89d5b6cbc2a563","source_url":"https://www.uniprot.org/uniprotkb/O75475","taxonomy_id":"9606","uniprot":"O75475"},"structure_contact_assay_links":[]}