{"assay":{"DMSO_percent":null,"abstract_reported_endpoint":null,"abstract_reported_method":null,"abstract_reported_units":null,"abstract_reported_value":null,"assay_construct_end":null,"assay_construct_start":null,"assay_record_id":"PWWP:ASSAY:0034","assay_type":"No pharmacological activity assigned","assay_value_transferred_to_modeled_stereoisomers":0,"audit_decision":"resolve_missing_CCD_to_BTB; retain_buffer_role","baseline_assay_record":1,"buffer":"Crystallization reservoir contains 0.1 M Bis-Tris; not an affinity assay","ccd_id":"BTB","ccd_mapping_source":null,"chemical_id":"INCHIKEY:OWMVSZAMULFTJU-UHFFFAOYSA-N","chemical_identity_status":"exact_single_CCD_identity","component_context":null,"compound_name":"Bis-Tris","conditions":"{}","crystallographic_component_ids":null,"domain":"PWWP","domain_id":"UP:Q9UBC3:PWWP1","endpoint_label_status":null,"evidence_class":"structural_occupancy_association","evidence_location":"Results: Trimethylated lysine histone recognition; crystallization methods","evidence_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3119473/","gene":"DNMT3B","interpretation":"Observed aromatic-cage occupancy by crystallization buffer. Do not classify as a validated pharmacological ligand.","molecular_entity_type":"buffer_additive","numeric_transcription_status":"not_a_quantitative_affinity_record","numeric_value_inferred_from_structure":0,"pH":6.5,"pdb_id":"3QKJ","preparation_id":"PREP:BIS-TRIS","protein_id":"UP:Q9UBC3","qualitative_result":null,"reference_id":"PMID:21720545","remaining_uncertainty":"Pocket occupancy in high-concentration crystallization buffer is not evidence of potency.","replicates":null,"role":"crystallization_buffer","source_component_url":null,"source_id":"S07","source_reported_uncertainty":null,"source_reported_units":null,"source_reported_value":null,"source_verification_scope":"local_primary_full_text_XML; Tables 1-2, crystallization methods and ligand Results inspected","temperature_C":null,"uncertainty":null,"uncertainty_type":null,"uncertainty_uM":null,"unit_conversion":null,"units":null,"value":null,"value_operator":null,"value_uM":null,"verification_level":"primary_full_text_sections_verified"},"source_verification":null}