{"assay":{"DMSO_percent":null,"abstract_reported_endpoint":null,"abstract_reported_method":null,"abstract_reported_units":null,"abstract_reported_value":null,"assay_construct_end":368,"assay_construct_start":208,"assay_record_id":"PWWP:ASSAY:0030","assay_type":"HTRF displacement IC50","assay_value_transferred_to_modeled_stereoisomers":0,"audit_decision":"retain_with_explicit_endpoint_and_identity","baseline_assay_record":1,"buffer":null,"ccd_id":null,"ccd_mapping_source":null,"chemical_id":null,"chemical_identity_status":"paper_label_exact_identity_unresolved","component_context":null,"compound_name":"compound 12 (Collie 2025)","conditions":"{\"assay_substitutions\": \"not listed for assay constructs; 9FOE crystallographic mutants kept separate\", \"phase3_verification\": \"SV05A/SV05B\", \"protein_construct\": \"NSD2 208\u2013368\", \"purified_tags\": \"N-terminal Avi; C-terminal 6His; biotinylated\", \"replicates\": 2, \"uncertainty_type\": \"SEM\"}","crystallographic_component_ids":null,"domain":"PWWP1","domain_id":"UP:O96028:PWWP1","endpoint_label_status":null,"evidence_class":"biochemical_interaction_inhibition","evidence_location":"Table 1","evidence_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","gene":"NSD2","interpretation":"Probe displacement from the H3K36me2 binding site; not catalytic SET-domain inhibition.","molecular_entity_type":"small_molecule_preparation","numeric_transcription_status":"matches_primary_report","numeric_value_inferred_from_structure":0,"pH":null,"pdb_id":null,"preparation_id":"PREP:COLLIE2025_12","protein_id":"UP:O96028","qualitative_result":null,"reference_id":"PMID:40959233","remaining_uncertainty":"HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.","replicates":"2","role":"experimentally_studied_ligand","source_component_url":null,"source_id":"S05","source_reported_uncertainty":"0.06","source_reported_units":"\u00b5M","source_reported_value":"2.49","source_verification_scope":"Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts.","temperature_C":null,"uncertainty":0.06,"uncertainty_type":"SEM","uncertainty_uM":0.06,"unit_conversion":null,"units":"\u00b5M","value":2.49,"value_operator":"=","value_uM":2.49,"verification_level":"primary_full_text_sections_verified"},"source_verification":{"assay_record_id":"PWWP:ASSAY:0030","baseline_source_id":"S05","compound_name":"compound 12 (Collie 2025)","field_rows":[{"field":"construct_bounds","locator":"Table S2, PDF page 7","note":"","source_id":"SV05B","source_scope":"Actual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked","source_url":"https://pubs.acs.org/doi/suppl/10.1021/acsmedchemlett.5c00396/suppl_file/ml5c00396_si_001.pdf","status":"verified","value":{"end":368,"numbering":"canonical UniProt inclusive","start":208}},{"field":"tags","locator":"Table S2, PDF page 7","note":"","source_id":"SV05B","source_scope":"Actual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked","source_url":"https://pubs.acs.org/doi/suppl/10.1021/acsmedchemlett.5c00396/suppl_file/ml5c00396_si_001.pdf","status":"verified","value":"N-terminal Avi; C-terminal 6His; biotinylated"},{"field":"engineered_mutations","locator":"Table S2, PDF page 7","note":"No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit.","source_id":"SV05B","source_scope":"Actual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked","source_url":"https://pubs.acs.org/doi/suppl/10.1021/acsmedchemlett.5c00396/suppl_file/ml5c00396_si_001.pdf","status":"not_reported","value":null},{"field":"assay_type","locator":"Table 1","note":"","source_id":"SV05A","source_scope":"Actual primary XML; full Results, Table 1 and footnotes inspected","source_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","status":"verified","value":"HTRF displacement IC50"},{"field":"temperature_C","locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","source_scope":"Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.","source_url":"https://doi.org/10.1021/acs.jmedchem.4c00215","status":"not_inspected","value":null},{"field":"buffer","locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","source_scope":"Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.","source_url":"https://doi.org/10.1021/acs.jmedchem.4c00215","status":"not_inspected","value":null},{"field":"pH","locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","source_scope":"Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.","source_url":"https://doi.org/10.1021/acs.jmedchem.4c00215","status":"not_inspected","value":null},{"field":"DMSO_percent","locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","source_scope":"Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.","source_url":"https://doi.org/10.1021/acs.jmedchem.4c00215","status":"not_inspected","value":null},{"field":"units","locator":"Table 1","note":"","source_id":"SV05A","source_scope":"Actual primary XML; full Results, Table 1 and footnotes inspected","source_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","status":"verified","value":"\u00b5M"},{"field":"measurement_uncertainty","locator":"Table 1","note":"Reported plus/minus magnitude, in the units of the assay result.","source_id":"SV05A","source_scope":"Actual primary XML; full Results, Table 1 and footnotes inspected","source_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","status":"verified","value":0.06},{"field":"uncertainty_type","locator":"Table 1 footnote b","note":"","source_id":"SV05A","source_scope":"Actual primary XML; full Results, Table 1 and footnotes inspected","source_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","status":"verified","value":"SEM"},{"field":"replicate_definition","locator":"Table 1 footnote b","note":"","source_id":"SV05A","source_scope":"Actual primary XML; full Results, Table 1 and footnotes inspected","source_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","status":"verified","value":{"definition":"replicates; independence/biological level not specified for HTRF in Table 1","n":2}},{"field":"measurement_entity","locator":"Table 1","note":"The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity.","source_id":"SV05A","source_scope":"Actual primary XML; full Results, Table 1 and footnotes inspected","source_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","status":"verified","value":"PREP:COLLIE2025_12"},{"field":"concentration_range","locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","source_scope":"Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.","source_url":"https://doi.org/10.1021/acs.jmedchem.4c00215","status":"not_inspected","value":null},{"field":"incubation_and_flow","locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","source_scope":"Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.","source_url":"https://doi.org/10.1021/acs.jmedchem.4c00215","status":"not_inspected","value":null},{"field":"fitting","locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","source_scope":"Bibliography, abstract and supporting-information description inspected. Final official repository checks found no PMC or Europe PMC full text. Direct ACS article retrieval returned HTTP 403; the full HTRF protocol remains uninspected. The actual supporting PDF was separately retrieved and inventoried as SV05D.","source_url":"https://doi.org/10.1021/acs.jmedchem.4c00215","status":"not_inspected","value":null},{"field":"negative_observation","locator":"Table 1","note":"","source_id":"SV05A","source_scope":"Actual primary XML; full Results, Table 1 and footnotes inspected","source_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12434523/","status":"not_applicable","value":null},{"field":"identity_assignment","locator":"Compounds 12/13, PDF page 14","note":"Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment.","source_id":"SV05B","source_scope":"Actual primary PDF obtained from official Europe PMC supplementary archive after direct ACS retrieval returned 403; Table S2, SPR, biochemical method and compound 11-13 chemistry visually checked","source_url":"https://pubs.acs.org/doi/suppl/10.1021/acsmedchemlett.5c00396/suppl_file/ml5c00396_si_001.pdf","status":"verified","value":{"absolute_sulfolane_configuration":null,"exact_ccd_id":null,"isomer_label":1,"level":"source-labelled separated fraction","reported_diastereomeric_ratio":"98.84:1.16","retention_time_min":7.1}}],"fields":{"DMSO_percent":{"locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","status":"not_inspected","value":null},"assay_type":{"locator":"Table 1","note":"","source_id":"SV05A","status":"verified","value":"HTRF displacement IC50"},"buffer":{"locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","status":"not_inspected","value":null},"concentration_range":{"locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","status":"not_inspected","value":null},"construct_bounds":{"locator":"Table S2, PDF page 7","note":"","source_id":"SV05B","status":"verified","value":{"end":368,"numbering":"canonical UniProt inclusive","start":208}},"engineered_mutations":{"locator":"Table S2, PDF page 7","note":"No substitutions are listed for these assay constructs. The separate crystallography construct for 9FOE lists seven mutations; those are not applied to assay records. This is not a sequence audit.","source_id":"SV05B","status":"not_reported","value":null},"fitting":{"locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","status":"not_inspected","value":null},"identity_assignment":{"locator":"Compounds 12/13, PDF page 14","note":"Purified fractions are not assumed enantiopure. Retention time is not an absolute stereochemical assignment.","source_id":"SV05B","status":"verified","value":{"absolute_sulfolane_configuration":null,"exact_ccd_id":null,"isomer_label":1,"level":"source-labelled separated fraction","reported_diastereomeric_ratio":"98.84:1.16","retention_time_min":7.1}},"incubation_and_flow":{"locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","status":"not_inspected","value":null},"measurement_entity":{"locator":"Table 1","note":"The reported result belongs to this experimental preparation and construct; structural CCD membership alone cannot transfer affinity.","source_id":"SV05A","status":"verified","value":"PREP:COLLIE2025_12"},"measurement_uncertainty":{"locator":"Table 1","note":"Reported plus/minus magnitude, in the units of the assay result.","source_id":"SV05A","status":"verified","value":0.06},"negative_observation":{"locator":"Table 1","note":"","source_id":"SV05A","status":"not_applicable","value":null},"pH":{"locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","status":"not_inspected","value":null},"replicate_definition":{"locator":"Table 1 footnote b","note":"","source_id":"SV05A","status":"verified","value":{"definition":"replicates; independence/biological level not specified for HTRF in Table 1","n":2}},"tags":{"locator":"Table S2, PDF page 7","note":"","source_id":"SV05B","status":"verified","value":"N-terminal Avi; C-terminal 6His; biotinylated"},"temperature_C":{"locator":"Cited full protocol, Carlino et al 2024; Collie SI PDF page 9","note":"Collie SI gives a short HTRF description and cites Carlino 2024. The main article returned HTTP 403 and is absent from PMC/Europe PMC. The actual Carlino supplement was retrieved and inventoried (SV05D), but no HTRF protocol was found there. The cited main protocol remains not inspected; SPR conditions are not transferred.","source_id":"SV05C","status":"not_inspected","value":null},"uncertainty_type":{"locator":"Table 1 footnote b","note":"","source_id":"SV05A","status":"verified","value":"SEM"},"units":{"locator":"Table 1","note":"","source_id":"SV05A","status":"verified","value":"\u00b5M"}},"gene":"NSD2","preparation_id":"PREP:COLLIE2025_12","verified_updates":{"conditions":{"assay_substitutions":"not listed for assay constructs; 9FOE crystallographic mutants kept separate","phase3_verification":"SV05A/SV05B","protein_construct":"NSD2 208\u2013368","purified_tags":"N-terminal Avi; C-terminal 6His; biotinylated","replicates":2,"uncertainty_type":"SEM"},"remaining_uncertainty":"HTRF full conditions are referenced to Carlino et al. 2024 DOI 10.1021/acs.jmedchem.4c00215. Its main protocol remains inaccessible after official repository checks. The actual supplement was inspected but contains no HTRF protocol. Table 1 reports SEM; independence level of HTRF replicates is not specified.","source_verification_scope":"Phase 3 primary Table 1 and actual Collie supporting PDF pages 7, 9 and 14; Carlino supporting PDF contents inspected, but the inherited main-article HTRF method remains not inspected after documented access attempts."}}}